Conclusiones La Ghrelina promueve la proliferación celular en

El Agro Veracruzano 2015
Conclusiones
La Ghrelina promueve la proliferación celular en Células madre de Cordón umbilical,
no se observó proliferación celular con el uso de un activador (Dibutiril AMPc) o un
bloqueador (KT5720) de la vía de la PKA, la Ghrelina activa la vía de la PKC y
ocupa el calcio extracelular para la activación de la vía CamK-II.
Literatura Citada
Beyer
N, Da Silva L. 2006. Mesenchymal Stem Cells: Isolation in vitro expansión and
characterization. Handb Exp Pharmacol ; 174: 249-282.
Bradford, M.M. 1976. Rapid and sensitive method for the quantitation of microgram quantities
of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
Cay Y. 2011. Cyclic nucleotide phosphodiesterase 1 regulates lysosome-dependent type I
collagen protein degradation in vascular smooth muscle cells: Arterioscler. Thromb.
Vasc. Biol. 31: 616-617
Chung H, Li E, Kim Y, Kim S, Park S. 2013. Multiple signaling pathways mediate ghrelininduced proliferation of hippocampal neural stem cells. J Endocrinol. 218(1):49-59.
Dornonville de la Cour C, Bjorkqvist M, Sandvik AK. 2001. A-like cells in the rat stomach
contain ghrelin and do not operate under gastrin control. Regul Pept. 99:141–150
Ezoe S. Matsumura I, Satoh Y, Tanaka H, Kanakura Y. 2004. Cell Cycle Regulation in
Hematopoietic Stem/Progenitor Cells. Cell Cycle.3: 314-318.
Freshney, I.R. 2010. Culture of Animal Cells: A Manual of Basic Technique and Specialized
Applications 6th ed. Editorial Wiley-Blackwell. 732.
Fujiwara, K. 1995. Effects of insulin, insulin-like growth factor-I, and phorbol esters on neutral
cholesteryl esterase activity in cultured rat vascular smooth muscle cellsLab. Clin.
Med. 26: 240.
Gualillo O, Caminos J, Blanco M. 2001 Ghrelin, a novel placental-derived hormone.
Endocrinology 142:788–794
Hoffman R, Benz E.J, Shattil S.J, Furie B, Cohen H.J, Silberstein L.E. 2000. Stem cell model of
hematopoiesis. En: Hematology: Basic principles and practice. Philadelphia, EE.UU.
Churchill Livingstone. 5 (1): 26 – 138.
Ivanova N, Dimos J.T, Schaniel C, Hackney J.A, Moore K.A, Lemischka I.R. 2002. A stem cell
molecular signature. Science.298: 601-604.
Jelkmann W.E. 2010. Regulation of erythropoietin production. J Physiol. 589:1251-8
Kagotani Y, Sakata I, Yamazaki M, Nakamura K, Hayashi Y, Kangawa K. 2001. Localization
of ghrelin-immunopositive cells in the rat hypothalamus and intestinal tract.
Proceedings of the 83rd Annual Meeting of The Endocrine Society. 337.
Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K.1999.Ghrelin is a growthhormone-releasing acylated peptide from stomach. Nature. 402: 656–660.
Kojima, M., Hosoda, H., Matsuo, H.,Kangawa, K. 2001.Ghrelin: discovery of the natural
endogenous ligand for the growth hormone secretagogue receptor. Trends in
Endocrinology and Metabolism.12: 118–122.
265