First Name: María-Eugenia

Transcripción

Curriculum vitae Gas López Mª Eugenia
April 2014
Contact Information:
Last Name: GAS LÓPEZ
First Name: María-Eugenia
Date of birth: October 25th, 1975
Place of birth: Alicante, Spain
Sex: Woman
Nationality: Spanish
e-mail address: [email protected]
Education:
04/12/2007 Doctorate Degree at the University of Valencia. Thesis directors: Dr. Ricardo
Flores Pedauyé, Dr. Carmen Hernández Fort and Dr. José-Antonio Daròs Arnau
(PhD developed at The Institute for Molecular and Cellular Plant Biology, CSIC,
Valencia, Spain. PhD. awarded with the highest possible grade).
2000-2001
Master of Research in Molecular Genetics and Evolution (DEA) at the
University of Valencia (Spain) (Final degree obtained: 9/10).
1998-1999 Teaching Aptitude Course, University of Valencia (Spain).
1993-1998 B. Sc. Degree in Biochemistry at the University of Valencia (Spain), 1993-1998.
(Final degree obtained: 8.24/10)
Research experience:
2009-2013 Postdoctoral researcher at the Institut de Génétique et de Biologie
Moléculaire et Cellulaire (IGBMC), from September to November. Illkirch.
Supervisor: Dr. Bertrand Séraphin. Research topic: mRNA decay, protein
interaction networks, and translation.
2008-2009 Postdoctoral researcher at the Centre de Génétique Moléculaire (CGM), from
September to August. Gif-sur-Yvette. Supervisor: Dr. Bertrand Séraphin.
Research topic: mRNA decay, protein interaction networks.
2007-2008 Senior Research Technician at the Valencian Institute for Agricultural Research
(IVIA), from September to August. Valencia (Spain). Supervisor: Dr. Luis
Navarro Lucas and Dr. Gema Ancillo Antón. Research topic: Flowering in
citrus.
2000-2007 PhD student at the Institute for Molecular and Cellular Plant Biology. Valencia
(Spain). This work period was interrupted during nine months (2002-2003) for
health reasons. Supervisor: Dr. Ricardo Flores Pedauyé, Dr.Carmen Hernández
Fort and Dr. José-Antonio Daròs Arnau. Research topic: Viroid replication.
1998-2000 Research graduate at the Department of Genetics of the University of Valencia
from October to May. Supervisor: Dr. Rosa de Frutos Illán and Dr. Luis Pascual
Calaforra. Research topic: Transposable elements.
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1995-1998 Research student at the Department of Genetics of the University of Valencia.
From February to September. Supervisor: Dr. Rosa de Frutos Illán and Dr. Luis
Pascual Calaforra. Research topic: Transposable elements.
PhD Fellowships
01/03/2005-28/02/2006
Spanish National Research Council and Bancaja Foundation
Program
15/06/2000-31/01/2005
Spanish Ministry of Science and Technology
Research Grants
01/06/2013-30/11/2013
Postdoctoral Research at Institut de Génétique et de Biologie
Moléculaire et Cellulaire (Illkirch, France). Funded by Centre
Européen de Recherche en Biologie et en Médecine GIE.
01/06/2011-31/05/2013
Moléculaire et Cellulaire (Illkirch, France). Funded by the County
Council Department of Education
01/04/2011-31/05/2011
Moléculaire et Cellulaire (Illkirch, France). Funded by Centre
Européen de Recherche en Biologie et en Médecine GIE.
01/09/2009-31/03/2010
Moléculaire et Cellulaire (Illkirch, France). Funded by Spanish
Ministry of Science and Innovation.
01/04/2009-31/08/2009 Postdoctoral Research at Centre de Génétique Moléculaire (Gif sur
Yvette, France). Funded by Spanish Ministry of Science and
Innovation.
01/09/2008-31/03/2009 Postdoctoral Research at Centre de Génétique Moléculaire (Gif sur
Yvette, France). Funded by National Center for Scientific Research
(CNRS).
15/01/2008-29/08/2008 Senior Research Technician at the Valencian Institute for
Agricultural Research (IVIA), Valencia (Spain). Funded by Dept of
Plant Protection and Biotechnology.
03/09/2007-28/12/2007 Senior Research Technician at the Valencian Institute for
Agricultural Research (IVIA), Valencia (Spain). Funded by Dept of
Plant Protection and Biotechnology.
List of publications:
Publications:
Peer-Reviewing Journals
- Francis SM, Gas ME, Daugeron MC, Bravo J, Seraphin B (2012) Structure of the Rbg1Tma46 complex reveals new functional domains and their role in polysome recuitment. Febs
Journal 279: 445-445
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- Francis SM, Gas ME, Daugeron MC, Bravo J, and Seraphin B (2012) Rbg1-Tma46 dimer
structure reveals new functional domains and their role in polysome recruitment. Nucleic
Acids Res, 40, 11100-11114.
- Gas ME and Seraphin B (2010) Twins take the job. EMBO J, 29, 2260-2261.
- Flores R, Gas ME, Molina-Serrano D, Nohales MA, Carbonell A, Gago S, De la Peña M,
and Daros JA (2009) Viroid replication: rolling-circles, enzymes and ribozymes. Viruses, 1,
317-334.
- van den Elzen AMG*, Henri J*, Lazar N, Gas ME, Durand D, Lacroute François, Nicaise
M, van Tilbeurgh H, Séraphin B, and Graille M (2010) Dissection of Dom34-Hbs1 reveals
independent functions in two RNA quality control pathways. Nat Struct Mol Biol, 17, 14461452.* Both authors contributed equally.
- Gas ME, Molina-Serrano D, Hernandez C, Flores R, and Daros JA (2008) Monomeric
linear RNA of citrus exocortis viroid resulting from processing in vivo has 5'phosphomonoester and 3'-hydroxyl termini: implications for the RNase and RNA ligase
involved in replication. J Virol, 82, 10321-10325.
- Gas ME, Hernández C, Flores R, and Daròs JA (2007) Processing of nuclear viroids in vivo:
an interplay between RNA conformations. PLoS Pathog, 3, 1813-1826.
- Flores R, Delgado S, Gas ME, Carbonell A, Molina D, Gago S, and De la Peña M. (2004)
Viroids: the minimal non-coding RNAs with autonomous replication. FEBS Lett, 567, 42-48.
- Martínez-Sebastián MJ, Hernández M, Mejias B, Gas ME, Pérez A, Pascual L, and de
Frutos R. (2002) Evolutionary patterns of the gypsy and bilbo retrotransposon families in the
Drosophila species of the obscura group. Mol Phylogenet Evol, 25, 254-266
Book chapters:
- Chlebowski A, Tomecki R, Gas ME, Séraphin B, and Dziembowski A (2010). Catalytic
properties of the eukaryotic exosome. (RNA Exosome) In Jensen TH (Ed.), Landes
Bioscience 702, 63-78.
- Flores R, Gas ME, Molina D, Hernández C, and Daròs JA (2008). Analysis of viroid
replication (Plant virology Protocols: From Viral Sequence to Protein Function). In Foster G,
Hong Y, Johansen E, and Nagy P (Eds.). Humana Press, Totowa (USA), pp. 167-183.
Participations in Meeting and Congresses:
- Poster presentation on Yeast DRG proteins: new proteins required for efficient translation
elongation in eukaryotes at 9th SifrARN Meeting. Annual Société Française de Biochimie et
Biologie Moléculaire Conference, (Bischenberg), 2013. Gas ME, Francis SM, Bravo J, and
Séraphin B. Prize for the best poster.
- Attendance to the EMBO conference “Eukaryotic RNA Turnover: From Structural Insights
to Diseases” (Strasbourg), 2013.
- Poster presentation on Rbg1-Tma46 dimer structure reveals new functional domain and their
role in polysome recruitment at 17th Annual Meeting of the RNA Society, Ann Arbor
(Michigan), 2012. Francis SM, Gas ME, Daugeron MC, Bravo J, and Séraphin B.
- Oral presentation on Mapping protein interaction networks; application to the RES complex
at the ESF RNA Quality workshop. A workshop for Phd students and post-docs working in
labs that participate in the European Science Foundation-RNA Quality Programme
(Strasbourg), 2010. Gas ME and Séraphin B.
- Poster presentation on Functional Study of transcription factors potentially involved in the
juvenile to adult phase transition in citrus at the XVIII Congress of the Federation of
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European Societies of Plant Biology, Valencia (Spain), 2010. Castillo MC, Gas ME, Navarro
L, Ancillo G.
- Oral presentation on Mapping protein interaction networks: application to the RES splicing
factor at TERM09 Meeting (Domaine de Seillac), 2009. Gas ME and Séraphin B.
- Poster presentation on RNA Processing during viroid replication at Workshops on Biology
(Replication and Recombination of RNA Virus Genomes), Cantoblanco (Spain), 2009.
Martínez F, Marqués J, Nohales MA, Gas ME, Molina-Serrano D, Flores R and Daròs JA.
- Poster presentation on Functional study of genes potentially involved in juvenile-to-adult
transition in citrus plants identified by gene expression profiling at 11th International Citrus
Congress, Wuhan (China), 2008. Ancillo G, Gas ME, Gadea J, Forment J, Juárez J, Marqués
MC, Pina JA and Navarro L.
- Oral presentation on Viroid RNA processing during replication at Viroids and viroid-like
RNAs 2008, Berlin (Germany), 2008. Gas ME, Molina-Serrano D, Martínez F, Marqués J,
Hernández C, Flores R and Daròs JA.
- Oral presentation on Processing of viroid RNAs during their replication at IX Congress of
Virology, Zaragoza (Spain), 2007. Gas ME, Molina-Serrano D, Marques J, Hernández C,
Flores R. and Daròs JA.
- Oral presentation on Termini of linear monomeric RNA, the resulting products of the
processing of dimeric transcripts of a nuclear viroid in transgenic plants: Inference about the
enzymes involved in the processing, at IX Spanish Congress of Virology, Zaragoza (Spain),
2007. Gas ME, Hernández C, Flores R and Daròs JA.
- Poster presentation on Processing of the viroid RNAs of the family Pospiviroidae in
transgenics plants, at the VIII Congress of Plant Molecular Biology, Pamplona (Spain), 2006.
Gas ME, Hernández C, Flores R and Daròs JA.
- Poster presentation on Viroid RNA processing during replication, at the II Meeting of
Ribored, Barcelona (Spain), 2006. Gas ME, Molina D, Hernández C, Flores R and Daròs JA.
- Poster presentation on Mapping the processing site of Citrus exocortis viroid oligomeric (+)
strands with an in vivo system at the XIII International Congress of Virology, International
Union of Microbiological Societies. San Francisco (California-USA), July 2005. Gas ME,
Hernández C, Flores R and Daròs JA.
- Oral presentation on Viroid replication, at the II European Congress of Virology, Madrid
(Spain), 2004. Flores R, de la Peña M, Gago S, Delgado S, Gas ME, Carbonell A, Martínez
de Alba E, Hernández C and Daròs JA.
- Attendance at the VII Spanish Congress of Virology, Valencia (Spain), 2001.
- Attendance at the X Congress of the Spanish Society of Phytopathology, Valencia (Spain),
2000.
- Poster presentation on Sequence homologous to the hobo in E strains of Drosophila
melanogaster at the II Congress of the Spanish Society of Genetic, A Coruña (Spain), 1999.
Gas ME, Sánchez MD, Galindo MI and Pascual L.
- Poster presentation on Phylogenetic analysis of Drosophila class I and II transposable
elements, at VII Congress of the European Society for Evolutionary Biology, Barcelona
(Spain), 1999. Mejias-Nogales B, Gas ME, Pérez-Muñoz A, Vázquez R, Gandia M,
Martínez-Sebastián MJ and de Frutos R.
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- Attendance at the I Congress of the Spanish Society of Genetic, Valencia (Spain), 1997.
Participation in funded research projects:
- Structural and functional analysis of Rbg1/Tma46 heterodimer. County Council Department
of Education (Programa VALi+d), from 2010 to 2012. PI: Bertrand Séraphin.
- Mechanism of exosome activation during cytoplasmic mRNA decay. CNRS, Spanish
Ministry of Science and Innovation, Centre Européen de Recherche en Biologie et en
Médecine GIE, and County Council Department of Education (Programa VALi+d), from
2009 to 2013. PI: Bertrand Séraphin.
- Functional study of genes potentially involved on citrus flowering. Institute for Agricultural
Research of Valencia, from 2007 to 2008. PI: Dr. Luis Navarro and Dr. Gema Ancillo.
- DNA microarray platform to study juvenile to adult transition in Citrus. Conselleria de
Empresa, Universidad y Ciencia de la Generalidad Valenciana, from 2005 to 2007. PI: Dr.
Gema Ancillo.
- Molecular and cellular viroids and plant viruses biology. Valencian Government, from 2003
to 2005. PI: Dr. Ricardo Flores.
- Viroids: enzymes, ribozymes and auxiliary factors involved in their replication, and in the
control of this pathogens through specific ribozymes. The Ministry of Science and
Technology of Spain, from 2002 to 2005. PI: Dr. Ricardo Flores.
- Cis-sequence and cis-factors involved in the replication cycle of viroids. The Ministry of
Education and Science of Spain, from 1999 to 2001. PI: Dr. Ricardo Flores.
Major fields of training
Yeast Saccharomyces cerevisiae, Plant Molecular Biology, Drosophila melanogaster, DNA
recombination techniques, RNA isolation, in vitro transcription assays, in vitro translation
assays, protein purification methods (TAP- Purification), protein interaction methods, in vitro
cross-linking experiments, protein purification methods (recombinant proteins, TAPPurification), polysome analysis and RNA and protein purification from sucrose gradients,
reporter gene assay, yeast two hybrid, Northern, and Western Blot techniques, tryptic
digestion of in-gel or in-solution proteins for mass spectrometry analysis, PCR, qPCR, and
RT-PCR, RNA ligase-mediated rapid amplification of cDNA end, site-directed and random
mutagenesis techniques, primer extension and DNA sequencing, SILAC, radioisotopes
manipulation, and data computer analysis.
Skills
Teamwork
I have successfully undertaken various team projects.
Communication
Oral communication skills expressed in presentations and writing
skills developed with grants application and grant reports, posters,
articles and chapter books.
Research collaboration
During my postdoctoral stay I collaborated with the group of Dr.
Bravo (Valencia, Spain).
Managing
Experience in managing research projects with well-defined goals,
resources and timescales.
Leaderships
Postdoctoral workers at the CGM and IGBMC are encouraged to
be independent and to take full scientific responsibility for their
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own projects, there being a special emphasis on preparing them to
become group-leading scientists.
Self-motivated
I am a very dynamic person.
Courses:
- Teaching Aptitude Course, University of Valencia (Spain), 1998-1999.
- VI International meeting about Human Genome: clonation and transplantation in the 21th
century”, International Menéndez Pelayo University, Valencia (Spain), 1999.
- Undergraduate practice in Clinical Biochemical Laboratory at Villajoyosa Hospital, Alicante
(Spain), 1997.
- Undergraduate practice in Clinical Biochemical laboratory at Dr. Pesset Aleixandre
Hospital, Valencia (Spain), 1997-1998.
- “Advanced course for molecular diagnostic techniques” Prince Felipe Research Centre,
Valencia (Spain) 1997.
- “Cellular damage and inflammatory response” Course of the Complutense University of
Madrid (Spain), 1996.
Languages:
- Mother tongue: Spanish.
- English: Advanced
- French: Intermediate
Other credits
- Member of Spanish Society of Biochemistry.
- Referee of the Doctoral thesis entitled “Molecular characterization and development of new
diagnostic methods for the genus Fabavirus. BTH evaluation as a control method” carried out
by the graduate student Ms. Rosa Mar Ferrer Gual under the guidance of Dr. José Guerri
Sirera, Dr. Pedro Moreno Gómez and Dr. Luis Rubio Miguélez (Valencian Institute for
Agricultural Research, Valencia, Spain).
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First Name: María-Eugenia

Transcripción

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