A reversed phase compatible thin layer chromatography

A reversed phase compatible thin layer chromatography

A reversed phase compatible thin layer chromatography-autography for the detection of
acetylcholinesterase inhibitors
I. Ayelen Ramallo,‡ Paula García‡ & Ricardo L. E. Furlan*
Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario (IIDEFAR/CONICETUNR), Universidad Nacional de Rosario, Ocampo y Esmeralda 2000 Rosario, Argentina.
‡These authors contributed equally to this work.
*Correspondence to: Ricardo L. E. Furlan. Instituto de Investigaciones para el Descubrimiento de
Fármacos de Rosario (IIDEFAR/CONICET-UNR), Ocampo y Esmeralda 2000 Rosario, Argentina.
e-mail: [email protected]
telephone: ++54 341 4237868
Table S1 Plackett–Burman design built for factor selection
Table S2 Central composite design used for the optimisation of the evaluated responses.
Table S3. Summary predicted values and experimental values.
Figure S1 Response surface plots for NSVIS: number of detected spots under visible light; NSUV:
number of detected spots under UV365nm light; IODVIS: integrated optical intensity (IOD) for 10 µg of
for physostigmine under visible light; IODUV: integrated optical intensity (IOD) for 10 µg of for
physostigmine under UV365nm light, as indicated.
Figure S2 Autography for detection of AChE inhibitors on Silica gel -TLC. From left to right, (1) 250
μg of Sonchus oleraceus butanol extract , (2) 250 μg of 0.01 % physostigmine skiped Sonchus
oleraceus butanol extract , and (3) physostigmine 0.025 μg(a) UV254nm light detection; (b) UV365nm
light detection; (c) AChE autography visualized under UV365nm light; (d) AChE autography visualized
under visible light. Elution was carried out with dichloromethane:methanol (60:40).