CV Miguel Lopez

Dr. Miguel Lopez Gomez
Plant Physiology Department
University of Granada
Fuentenueva s/n
18071-Granada
Spain
Tel: +34 958243382
Fax: +34 958248995
[email protected]
Professional Experience
Job category
Period
Institution
Topic
Post-doctoral
Research Associate
Jan-Jul.
2008
Plant Physiology Department,
University of Granada, Spain
Symbiosis rhizobium-legume
under salt stress
Post-doctoral
Training Fellow
Aug 2008/
Mar 2009
Molecular Microbiology
Department, John Innes Centre,
Norwich, England
Energisation of nitrogen fixation
in the rhizobium-legume
symbiosis
Post-doctoral
Fellow
Apr 2009/
now
Botanical Institute, University of
Basel, Switzerland
Discrimination between
symbiotic and pathogenic
microorganism in legumes
Education/Training
Studies of Biology. 2001. University of Granada, Spain
Pre-graduate fellowship. 2000/2001. Biochemistry Department, University of Granada, Spain.
Diploma (MSc). 2005. Biología Agraria y Acuicultura. University of Granada, Spain.
PhD Thesis. 2007. University of Granada, Spain.
Supervised by Dra. Carmen Lluch Plá and Dr. José A. Herrera-Cervera.
Nodular carbon metabolism in the symbiosis Medicago truncatula-Sinorhizobium meliloti, Lotus japonicusMesorhizobium loti under salt stress: role of trehalose.
Visits to other Institutions
Institute: Institute of Forest Genetics, Universidad Veracruzana
City: Xalapa, Mexico
Work performed: Mycropropagation of Pinus species
Date: 3-9-2001/29-10-2001
Institute: Ohio Agricultural and Development Center (OARDC), Ohio State University
City: Wooster (Ohio), USA
Work performed: Trehalose metabolism in Sinorhizobium meliloti and Mesorhizobium loti under salt stress
conditions
Date: 15-9-2005/15-12-2005
Institute: Ohio Agricultural and Development Center (OARDC), Ohio State University
City: Wooster (Ohio), USA
Work performed: Trehalose metabolism in Bradyrhizobium japonicum and soybean nodules under salt
stress conditions
Date: 15-6-2006/15-9-2006
1
Summary of Past and Present Research
Pre doctoral research
My research trajectory has been focused in plant-microbe interactions and specifically in the symbiotic
interaction of legumes with soil bacteria known as rhizobia. In this interaction, the bacteria reduce
atmospheric dinitrogen to ammonia which is supplied to the plant that provide the appropriate conditions
allowing this process to takes place. My Master Thesis and PhD Thesis projects consisted in the study of
nitrogen fixation under salt stress conditions in the model legumes Lotus japonicus and Medicago truncatula.
Those studies were approached from two different perspectives: a) the effects of salinity on the cellular
osmotic potential by determining the biochemical and physiological mechanisms involved in salt adaptation
such as accumulation of compatible osmolytes, b) the nutrient balance by the analysis of the effect of high
salinity in the nutrient distribution in the plant. The negative effect of salt stress was also determined in other
plant major processes such as cell respiration, photosynthetic activity and nitrogen and carbon metabolism.
Post doctoral research
Since April of 2009 I’m working in a two years project funded by the Spanish Education Ministry and the
Swiss National Foundation in the Botanical Institute of the University of Basel (Switzerland). The aim of this
project is to determine the capacity of legumes to discriminate between symbiotic and pathogenic
microorganisms since it is known the availability of plants to recognize potentially pathogenic
microorganisms and mount an efficient defence response. During the last months I have been working in the
immune responses of model legume Lotus japonicus upon treatment with different elicitors, and how they
affect the establishment of nitrogen fixation symbiosis in order to determine a possible cross-talk between
the signalling pathways involved in the symbiosis and innate immunity.
2
Publications
Articles:
1
N.A. Tejera, C. Iribarne, M. López, J. Herrera-Cervera y C. Lluch
Physiological implications of trehalase from Phaseolus vulgaris root nodules: partial purification and
characterization
Plant Physiology and Biochemistry 43, 355-361, 2005
2
J.G. Streeter, M. L. Gómez
Three enzymes for trehalose synthesis in Bradyrhizobium cultured bacteria and bacteroids from soybean
nodules
Applied and Environmental Microbiology 72, 4250-4255, 2006
3
M. López, J.A. Herrera-Cervera, C. Lluch and N.A. Tejera
Trehalose metabolism in root nodules of the model legume Lotus japonicus in response to salt stress
Physiologia Plantarum 128 (4), 701–709, 2006
4
Miguel López, Jose A. Herrera-Cervera, Carmen Iribarne, Noel A. Tejera, Carmen Lluch
Growth and nitrogen fixation in Lotus japonicus and Medicago truncatula under NaCl: Nodule carbon
metabolism
Journal of Plant Physiology 165, 641-650, 2008
5
Miguel López, Carmen Lluch
Nitrogen fixation is synchronized with carbon metabolism in Lotus japonicus and Medicago truncatula
nodules under salt stress
Journal of Plant Interactions 3 (3), 137-144, 2008
6
Miguel López, Noel A. Tejera and Carmen Lluch
Differential strategies of the Model Legumes Lotus japonicus and Medicago truncatula in the Adaptation
to Salt Stress: Photosynthetic and Nutritional Responses
American Journal of Plant Physiology 3 (3), 121-130, 2008
7
Miguel López, Noel A. Tejera, Carmen Iribarne, Carmen Lluch and José A. Herrera-Cervera
Trehalose and trehalase in root nodules of Medicago truncatula and Phaseolus vulgaris in response to
salt stress
Physiologia Plantarum 134, 575-582, 2008
8
Miguel López, Noel A. Tejera, Carmen Lluch
Validamycin A improves the response to salt stress of Medicago truncatula by inducing trehalose
accumulation in the root nodules
Journal of Plant Physiology 166, 1218-1222, 2009
9
Jurgen Prell, Alexandre Bourdès, Ramakrishnan Karunakaran, Miguel Lopez-Gomez, Philip Poole
Pathway of γ-aminobutyrate (GABA) metabolism in Rhizobium leguminosarum 3841 and its role in
symbiosis
Journal of Bacteriology 191 (7), 2177-2186, 2009
10 Geraldine Mulley, Miguel Lopez-Gomez, Ye Zhang, Jason Terpolilli, Jurgen Prell, Turlough Finan and
Philip Poole
Pyruvate is formed by two pathways in pea bacteroids with different efficiencies for nitrogen fixation
Journal of Bacteriology. 192 (19), 4944-4953, 2010
11 Miguel Lopez-Gomez, Noel A. Tejera, Carmen Iribarne, Jose A. Herrera-Cervera and Carmen Lluch
Different strategies for salt tolerance in determined and Indeterminate nodules of Lotus japonicus and
Medicago truncatula
Archives of Agronomy and Soil Science. In press
3
Book chapters:
1
M. Olivera, M. López, C. Iribarne, A. Ocaña y N.A. Tejera
"Metabolismo del amonio en nódulos de Phaseolus vulgaris: efecto del fósforo"
Book title: Avances en el Metabolismo del Nitrógeno: de los Microorganismos a las Plantas.
Editors: J.M. Vega Piqueres, A.J. Marquez Cabeza, A.J. Vigara, C. Vilchez Lobato, I. Garbayo Nores.
Edited by Academia de Ciencias, Artes y Letras de Huelva y Centro Asociado de la UNED en Huelva,
pg 347-356, 2005
ISBN 84-96-373-55-X.
2
Noel A. Tejera, Francisco Palma, Miguel López, José A. Herrera-Cervera, Carmen Lluch
“Respuestas en la simbiosis Rhizobium-leguminosa a factores abióticos y nutricionales”
Book title: Nutrición mineral. Aspectos fisiológicos, agronómicos y ambientales
Editors: C. Lamsfus Arrien, P.M. Aparicio Tejo, C. Arrese-Igor, I. Irigoyen Iriarte, J.F. Morán Juez.
Edited by Universidad pública de Navarra, pg 53-58, 2006
ISBN 84-9769-165-2
3
Noel A. Tejera, José A. Herrera-Cervera, Carmen Iribarne, Miguel López, Antonio Ocaña
“Nutricional alterations of the chickpea-M. ciceri symbiosis in response to salt stress”
Book title: Nutrición mineral. Aspectos fisiológicos, agronómicos y ambientales
Editors: C. Lamsfus Arrien, P.M. Aparicio Tejo, C. Arrese-Igor Sánchez, I. Irigoyen Iriarte, J.F. Morán
Juez.
Edited by Universidad pública de Navarra, pg 83-90, 2006
ISBN 84-9769-165-2
4
Miguel López and Carmen Lluch
“Trehalose in plant-microorganism symbiotic interactions”
Book title: Biochemical responses of plants to invaders
Editors: M.E. Legaz, C.V. Córdoba
Edited by Research Signpost, Kerala, India. 2008
ISBN 978-81-308-0306-7
5
Miguel López y Carmen Lluch
“Solutos compatibles nitrogenados vs. carbonados en la adaptación al estrés salino de Lotus japonicus
en simbiosis con Mesorhizobium loti”
Book title: Avances en el metabolismo del nitrógeno. De la genómica y la proteómica a las aplicaciones
agronómicas, industriales y medioambientales
Editors: Maria José Bonete, Rosa Mª Martinez Espinosa
Edited by Editorial Club Universitario, pg 311-318, 2009
ISBN 978-84-8454-8065
6
M López, NA Tejera, FJ Palma, C Lluch
“Mecanismos de Adaptación al Estrés Salino en dos Leguminosas Modelo: Respuestas Nutricionales”
Book title: Presente y Futuro de la Nutrición Mineral de las Plantas
Editors: Luis Romero Monreal (Editor-Coordinador), Juan Manuel Ruiz, Begoña Blasco, María del Mar
Rubio Wilhelmi, Eva Sánchez Rodríguez, Juan JoséRíos, Luis Miguel Cervilla
ISBN 978-84-89780-10-7
7
FJ Palma, C Iribarne, M López, NA Tejera, C Lluch
“Estudio de la Simbiosis Sinorizobium meliloti-Medicago sativa: Fijación de Nitrógeno y Metabolismo
Carbonado en Nódulos”
Book title: Presente y Futuro de la Nutrición Mineral de las Plantas
Editors: Luis Romero Monreal (Editor-Coordinador), Juan Manuel Ruiz, Begoña Blasco, María del Mar
Rubio Wilhelmi, Eva Sánchez Rodríguez, Juan JoséRíos, Luis Miguel Cervilla
ISBN 978-84-89780-10-7
4
Participation in Conferences and Meetings
International
1
López-Gomez Miguel and Thomas Boller
Poster
Innate immunity versus symbiosis in the interaction between Lotus japonicus and Mesorhizobium loti
Model Legume Congress 2011-05-12
Sainte Maxime, France. 15-19 May 2011
2
López-Gomez Miguel and Thomas Boller
Poster
Innate immunity and symbiotic interaction in Lotus japonicus: effect of flg22 on the symbiotic
establishment
9th European Nitrogen Fixation Conference
Geneva, Switzerland. 6-10 September 2010
3
López M, Iribarne C, Palma F, Herrera-Cervera JÁ, Lluch C
Poster
Implicación de la trehalosa y trehalasa en la adaptación al estrés salino en nódulos de leguminosas
I Congreso Luso-Español de Fijación de Nitrógeno y XII Reunión Nacional de Fijación de Nitrógeno
Estoril, Portugal. 1-4 Junio 2008
4
Tejera NA, Palma F, López M y Lluch C
Poster
Respuestas antioxidantes inducidas por ácido salicílico y NaCl en la simbiosis Phaseolus vulgarisRhizobium tropici
I Congreso Luso-Español de Fijación de Nitrógeno y XII Reunión Nacional de Fijación de Nitrógeno
Estoril, Portugal. 1-4 Junio 2008
5
Carmen Lluch, Miguel López, José R Barranco Gresa, Noel Tejera, José A Herrera-Cervera
Talk
Factores abióticos que limitan la fijación de nitrógeno en leguminosas: contribución de los organismos
rizoféricos
XXIII Reunión Latinoamericana de Rizobiologia
Los Cocos-Córdoba, Argentina. 25-29 Marzo 2007
6
Carmen Lluch, Miguel López, José R Barranco Gresa, Noel Tejera, José A Herrera-Cervera
Talk
Factores abióticos que limitan la fijación de nitrógeno en leguminosas: contribución de los organismos
rizoféricos
Workshop Inoculantes en Iberoamérica Necesidades y Oportunidades
Córdoba, Argentina. 29 Marzo 2007
7
López, Miguel, Tejera, Noel A, Pieckenstain, Fernando, Lluch, Carmen, Herrera-Cervera, Jose A
Poster
Trehalose metabolism in nodules of Medicago truncatula under salt stress
Join Annual Meeting of the American Society of Plant Biologists and the Canadian Society of Plant
Physiologist
Boston, Massachusetts, USA. 5-9 Agosto 2006
8
Miguel López, Noel A Tejera, José Roman Barranco-Gresa, Carmen Lluch, José A. Herrera-Cervera
Poster
Trehalose metabolism in nodules of Lotus japonicus under salt stress: effect of Validamycin A
th
7 European Nitrogen Fixation Conference
University of Aarhus, Dinamarca. 22-26 Julio 2006
5
9
Pieckenstein FL, López M, Tejera NA, Herrera-Cervera JA, Lluch C, Ruiz O
Poster
Cambios inducidos por la salinidad en los niveles de poliaminas de plantas de Lotus japonicus en
simbiosis con Mesorhizobium loti
IX Congreso Luso-Español de Fisiología Vegetal
Évora, Portugal. 18-21 Septiembre 2005
10 López M., Iribarne C, Barranco JR, Pieckenstein FL, Herrera-Cervera JA
Poster
Aspectos bioquímicos y moleculares del metabolismo de la trehalosa en nódulos de Medicago
truncatula: efecto del tratamiento salino
IX Congreso Luso-Español de Fisiología Vegetal
Évora, Portugal. 18-21 Septiembre 2005
11 José A Herrera-Cervera, Miguel López, Noel Tejera, Carmen Lluch
Poster
Partial cloning and expression analysis under salt stress of MTTRE1, a Medicago truncatula gene coding
for trehalase
Annual Main Meeting of the Society for Experimental Biology
Universitat Autonoma de Barcelona. 11-15 Julio 2005
12 Jose A Herrera-Cervera, Miguel López, Noel Tejera, Juán Sanjuán, Carmen Lluch
Poster
Trehalase metabolism and insolation of parcial c DNA of tehalase gene in Medicago truncatula
6th European Nitrogen Fixation Conference
Toulouse, France. 24- 27 Julio 2004
National
1
Miguel López, John G. Streeter, Carmen Iribarne, Francisco Palma, Noel A Tejera y Carmen Lluch
Poster
Metabolismo de la trehalosa en la adaptación al estrés salino de la simbiosis Glycine maxBradyrhizobium japonicum
XVIII Reunión de la Sociedad Española de Fisiología Vegetal (SEFV)
Zaragoza, Spain. 8-11 Septiembre 2009
2
Miguel López, Noel A Tejera y Carmen Lluch
Poster
Respuesta nutricional en Lotus japonicus y Medicago truncatula en la adaptación al estrés salino
XII Simposio Ibérico sobre Nutrición Mineral de las Plantas
Granada, Spain. 22-24 Octubre 2008
3
Miguel López y Carmen Lluch
Poster
Solutos compatibles nitrogenados vs. carbonados en la adaptación al estrés salino de Lotus japonicus
en simbiosis con Mesorhizobium loti
IX Reunión Nacional del Metabolismo del Nitrógeno
Alicante, Spain. 23-25 Abril 2008
4
M López Gómez, C Iribarme, J Román Barranco, JA Herrera-Cervera, C Lluch Plá
Poster
Papel de la trehalosa en los mecanismos de adaptación del metabolismo carbonado nodular al estrés
salino en nódulos de M. truncatula y L. japonicus
XVII Reunión de la Sociedad Española de Fisiología Vegetal-X Congreso Hispano-Luso de Fisiología
Vegetal
Alcalá de Henares, Spain. 18-21 Septiembre 2007
6
5
Carmen Lluch, Noel Tejera, José A Herrera-Cervera, Miguel López, José R Barranco-Gresa, Francisco
J. Palma, Mónica Gozálvez, Carmen Iribarne, Emilio Moreno, Antonio Ocaña
Talk
Tolerancia al estrés salino en leguminosas
Workshop: Lotus como leguminosa modelo y alternativa sustentable para la recuperación de áreas
marginales
Valencia, Spain. 6-7 Septiembre 2007
6
Noel Tejera, José A. Herrera-Cervera, Carmen Iribarne, Miguel López y Antonio Ocaña
Poster
Effect of salinity in nutricional responses of five chickpea cultivars
XI Simposio Ibérico de Nutrición Mineral de las Plantas-NUTRIPLANT 2006
Pamplona, Spain. 19-21 Julio 2006
7
Noel Tejera, Francisco Palma, Miguel López, José A. Herrera-Cervera y Carmen Lluch
Poster
Avances en la simbiosis Rhizobium–leguminosa: receptores, transportadores y factores abióticos que
limitan la fijación de nitrógeno
XI Simposio Ibérico de Nutrición Mineral de las Plantas-NUTRIPLANT 2006
Pamplona, Spain. 19-21 Julio 2006
8
López M, Herrera-Cervera JA, Sanjuán J, Lluch C y Streeter JG
Talk
Papel de la trehalosa en los mecanismos de adaptación al estrés salino en Sinorhizobium meliloti y
Mesorhizobium loti en vida libre
XI Reunión Nacional de Fijación de Nitrógeno
El Escorial, Spain. 5-8 Junio 2006
9
López M, Herrera-Cervera JA, Iribarne C, Ocaña A, Lluch C
Talk
Actividad trehalasa y metabolismo carbonado en nódulos de Medicago truncatula bajo condiciones de
estrés salino
X Reunión Nacional de Fijación de Nitrógeno
Granada, Spain. 15-18 Junio 2004
10 Herrera-Cervera JA, López M, Tejera NA, Lluch C
Talk
Clonación y expresión del gen que codifica para trehalasa en Medicago truncatula.
X Reunión Nacional de Fijación de Nitrógeno
Granada, Spain. 15-18 Junio 2004
11 Tejera N, Iribarne C, López M, Herrera-Cervera JA, Lluch, C
Poster
Purificación y caracterización de la enzima trehalasa nodular de Phaseolus vulgaris
X Reunión Nacional de Fijación de Nitrógeno
Granada, Spain. 15-18 Junio 2004
1
12 Marleni Olivera, Miguel López, Carmen Iribarne, Antonio Ocaña, Noel Tejera
Talk
Metabolismo del amonio en nódulos de Phaseolus vulgaris: efecto del fósforo
Congreso: VII Reunión Nacional del Metabolismo del Nitrógeno
Almonte (Huelva), Spain. 12-14 Febrero 2004
7
Teaching Activity
Position
Pre-doctoral fellow
Pre-doctoral fellow
Substitute teaching staff
Substitute teaching staff
Pos-doctoral research associate
Pos-doctoral research
Subject
Plant Physiology
6 credits. Practical Courses
Plant Physiology
6 credits. Practical Courses
Plant Ecophysiology
2.5 credits. Lectures
Plant Biotechnology
1.5 credits. Practical Courses
Plant Physiology
6 credits. Practical Courses
Intensiv Kurs in Symbiosen
2 credits. Practical Courses
Languages
Spanish: Native language
English: Excellent at writing and speaking
German: Basic level
Referees
Dra. Carmen Lluch Plá
University of Granada
Facultad de Ciencias
Fuentenueva s/n
18071 Granada, Spain
Tf +34 958243382
[email protected]
Dr. Philip Poole
John Innes Centre
Norwich Research Park
Colney, Norwich NR4 7UH, UK
Tf +44 (0)1603 450750
[email protected]
Dr. Thomas Boller
Botanical Institute
University of Basel
Hebelstrasse 1
4056 Basel, Switzerland
Tf +41 61 267 23 20
[email protected]
8
Institution
Year
University of Granada
2005/2006
University of Granada
2006/2007
University of Granada
2007/2008
University of Granada
2007/2008
University of Granada
2007/2008
University of Basel
2009/2010