fapronatura 2009 - Sociedad Cubana de Farmacología

Transcripción

Sociedad Cubana de Farmacología /
Cuban Society of Pharmacology
Segundo Simposio Internacional sobre Farmacología de
Productos Naturales /
Second International Symposium on Pharmacology of
Natural Products
FAPRONATURA 2009
RESÚMENES /
ABSTRACTS
Hotel OASIS Varadero 1920, Cuba
June 3-7, 2009
“La Naturaleza al Servicio de la Salud” /
“The Nature to the Service of the Health”
WEB site
http://www.scf.sld.cu
FAPRONATURA 2009
Second International Symposia on Pharmacology
of Natural Products
Varadero, Cuba. June 3-7, 2009
Revista Cubana de Plantas Medicinales vol. 14 (Suplemento Especial):ii, 2009
Edición, diseño y realización:
Dr. René Delgado Hernández
MSc. Ricardo Castro Armas
Consejo Científico Editorial:
Dr. René Delgado Hernández.
MSc. Ricardo Castro Armas
MSc. Marbel Guilarte Legrá
© Sociedad Cubana de Farmacología.
© Revista Cubana de Plantas Medicinales
© Sobre la presente edición: Editorial Ciencias Médicas, mayo,
2009.
RNPS 1827
ISSN 1028-4796
Impresión:
Taller de imprenta del Buró de Eventos del Ministerio de Salud Pública,
Calle 2, No. 352 entre 15 y 17, Vedado, Ciudad de La Habana, Cuba.
Teléfono: 830-5252 extensión 231.
N.E.: Todos los derechos reservados. Esta revista (o cualesquiera de sus partes) no puede ser copiada,
reproducida, almacenada, grabada o trasmitida por ningún medio electrónico, mecánico u otros, sin
permiso previo escrito de los patrocinadores.
La información en todas las partes del libro ha sido reproducida tal y como fue aportada por sus autores
respectivos.
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Revista Cubana de Plantas Medicinales vol. 14 (Suplemento Especial):iii, 2009
Presidente de Honor / Honor President
Dr. José R. Balaguer Cabrera
Dra. Concepción Campa Huergo
Comité Organizador / Organizer Committee
Dr. René Delgado Hernández, CENCEC
Dra. Idania Rodeiro Guerra, CEBIMAR
Dra. Diadelis Remírez Figueredo, CECMED
MSc Maria Cristina Lara Bastanzuri, CDF
MSc. Miguel D. Fernández Pérez, CEBIMAR
MSc. Mario Landys Chovel Cuervo, Inst Finlay
Dr Elisa Aznar, BIOCEN
Dra. Martha Pérez Viña, CENAMENT
MSc. Rodolfo López, IFAL
MSc. Yahelin Ferrer, IFAL
Comité Científico / Scientific Committee
Dra. Idania Rodeiro Guerra, CEBIMAR
Dr. Roberto Menendez Soto del Valle, CEBIMAR
Dra. Diadelis Remírez Figueredo, CECMED
Dra. Mariela M. Guevara García, CECMED
Dra. María A. Marrero Migaraya, CENCEC
Dra. Barbara Beatriz Garrido Suarez, CENCEC
Dra. Giset Jiménez López, CDF
Prof. Evangelina Marrero Faz, CENSA
MSc. Ricardo Castro Armas, CIDEM
Comité Asesor Científico Internacional / Internacional Scientific Advisory Committee
Elizabeth M. Williamson, Reading University, UK
Michael Heinrich, London University, UK
José M. Prieto, London University, UK
Fabio Firenzuoli, Natural Medicine Center, Empoli, Italy
Adrea Zangara, Flordis Company, Australia
Guy Haegeman, University of Gent, Belgium
Bertha Prieto, Facultad de Medicina, UNAM, México
Comité de Aseguramiento / Assurance Committee
Amarilis Noa Mederos, CDF
Marbel Guilarte Legra, ELAM
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Patrocinadores / Sponsors
Ministerio de Salud Pública (MINSAP)
Ministerio de la Industria Básica (MINBAS)
Consejo Nacional de Sociedades Científicas de la Salud (CNSCS)
Sociedad Cubana de Farmacología (SCF)
Centro para el Desarrollo de la Farmacoepidemiología (CDF)
Centro Nacional para el Control de la Calidad de los Medicamentos (CECMED)
Centro Nacional Coordinador de Ensayos Clínicos (CENCEC)
Centro Nacional de Sanidad Agropecuaria (CENSA)
Centro de Investigación y Desarrollo de Medicamentos (CIDEM)
Centro de Bioproductos Marinos (CEBIMAR)
Centro Nacional de Información en Ciencias Médicas (Infomed)
Centro Nacional de Medicina Natural y Tradicional (CENAMENT)
Universidad de La Habana, Instituto de Farmacia y Alimentos
Instituto de Vacunas Finlay
Empresa QUIMEFA
Laboratorios LABIOFAM
Centro de Biopreparados (BIOCEN)
Ministry of Public Health
Ministry of Basic Industry
National Council of Scientific Society f Health
Cuban Society of Pharmacology
Center for the Development of the Pharmacoepidemiology (CDF)
Center for the Control & Quality of Drugs (CECMED)
National Coordinating Center for Clinical Trials (CENCEC)
National Center for Animal and Plant Health (CENSA)
Drug Research and Development Center (CIDEM)
Center of Marine BioProducts (CEBIMAR)
National Center of Information in Medical Sciences (INFOMED)
National Center of Traditional and Natural Medicine (CENAMENT)
Havana University, Institute of Pharmacy and Food (IFAL-UH)
Finlay Vaccines Institute
QUIMEFA
Pharmaceutical Laboratories LABIOFAM,
Center of Bioproducts BIOCEN
Laboratorios Farmacéuticos y Empresas Extranjeras
Pharmaceutical Laboratories and Foreingner Companies
AMPELOS, BAYER CONSUMER CARE LTD, NOVARTIS,
BAYER, GAUTIER-BAGÓ, ROCHE, ASTRA–ZENECA
SEPPIM-INNOTECH, MEDICARIBE, SANOFI AVENTIS, MEDAVAN,
MERCK, SERVIER, FLORDIS NATURAL MEDICINES,
GINSANA, LUMEN MARKETING COMPANY,
LOS PORTALES S.A., HABANOS S.A
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PRÓLOGO
La Sociedad Cubana de Farmacología convoca a sus asociados y profesionales relacionados con el quehacer
de las ciencias farmacológicas en todo el país y a nivel internacional a la celebración del Segundo Simposio
Internacional sobre Farmacología de los Productos Naturales.
Este segunda edición del Simposio, después de tres anos en que se desarrollo el primero, constituye una
oportunidad excepcional para profundizar en el conocimiento, fortalecer el intercambio científico y la
colaboración entre profesionales que desarrollan su actividad diaria en el campo de las ciencias farmacológicas
y otras disciplinas relacionadas, en función de los productos naturales. Este es un ejemplo de cuánto se puede
lograr en este campo de las ciencias cuando se trabaja con voluntad y máxima dedicación a una obra científica
de importancia para el desarrollo de nuestros pueblos y sus culturas.
Alrededor de 100 investigadores cubanos, provenientes de universidades, facultades de ciencias médicas,
institutos de investigación, centros asistenciales, hospitales y farmacias comunitarias participan en este evento.
Esto representa un salto cualitativo para la Sociedad Cubana de Farmacología, al tratarse de un foro
enmarcado en una temática tan específica, la farmacología de los productos naturales. Todo ello pone de
manifiesto la importancia de la realización de estas actividades científicas como parte de las estrategias que se
desarrollan en el país para llevar a cabo la integración, colaboración y actualización científica de los
profesionales que trabajan en este importante campo de las investigaciones farmacológicas.
Es necesario destacar la participación de más de 50 personalidades científicas de Argentina, Bélgica, Bolivia,
Brasil, Canadá, Ecuador, España, Eslovaquia, Eslovenia, Estados Unidos, India, Italia, México, Pakistán, Perú,
Polonia, Reino Unido, República de Sudáfrica, Suiza, Turquía y Venezuela, quienes con su presencia en
nuestro país, la calidad de los trabajos enviados y sus continuos mensajes de apoyo y solidaridad hacia
nuestros científicos, nuestro pueblo y en particular a la labor organizativa de este Foro, ya dejan una huella
imborrable para la historia de estos eventos científicos.
Los más de 200 trabajos entre conferencias, comunicaciones orales, simposios, y presentaciones en carteles;
así como el debate, el análisis e intercambio recíproco darán relieve a esta actividad. También, ellos permitirán
trasladar a las instituciones de los participantes en más de 20 países, los conocimientos adquiridos, la
necesidad de continuar en la búsqueda de nuevas soluciones y el necesario incremento del papel de los
especialistas en farmacología y ciencias afines en el desarrollo de nuevos fármacos a partir de los productos
naturales.
Bienvenidos todos al inigualable escenario de este Simposio, la playa más hermosa de Cuba, Varadero.
Estamos convencidos que este encuentro permitirá crear nuevas perspectivas para el trabajo futuro y
consolidará aun más la actividad científica de todos los participantes aquí reunidos.
Muchas gracias a todos por su participación.
Presidente del Comité Organizador
FAPRONATURA 2009
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PREFACE
The Cuban Society of Pharmacology calls its associates and professionals related with the pharmacological
sciences’ work at international level to the celebration of the First International Symposia of Pharmacology of the
Natural Products.
These second Symposium constitute an exceptional opportunity to deepen in the knowledge, to strengthen the
scientific exchange and the collaboration among professionals that develop their daily activity in the field of the
pharmacological sciences and other related disciplines, in function of the natural products. This is an example of
how much it can achieve in this field of the sciences with maximal dedication to an important scientific work for
the development of our peoples and their cultures.
Around 100 Cuban researchers, coming from universities, faculty of medical sciences, investigation institutes,
assistance centers, hospitals and community pharmacies participate in this event. This represents a qualitative
jump for the Cuban Society of Pharmacology, when being a forum framed in a so specific thematic, the
pharmacology of natural products. All of this shows the importance to the realization of these scientific activities
as part of the strategies that are developed in the country to carry out the integration, collaboration and the
professionals' scientific update those that work on this important field of the pharmacological investigations.
It is necessary to highlight the participation of more than 50 scientific personalities from Argentina, Belgium,
Bolivia, Brazil, Canada, India, Italy, Mexico, Pakistan, Perú, Poland, Republic of South Africa, Slovenia, Spain,
Switzerland, Turkey, United States, United Kingdom and Venezuela, who with their presence in our country, the
quality of the sent works and their continuous support messages and solidarity toward our scientists, our people
and, in particular, to the organizational work of this Forum, already leave an indelible print for the history of these
scientific events.
More than 200 works among conferences, oral communications, symposia, and presentations in posters; as well
as the debate, the analysis and reciprocal exchange will give relief to this activity. They also will allow
transferring to the participant’s institutions in more than 30 countries the acquired knowledge, the need to
continue in the search of new solutions and the necessary increment of the specialist’s paper in pharmacology
and other sciences in the development of new medicines starting from the natural products.
Welcome to everybody to the wonderful scenario of these Symposia, the most beautiful beach in Cuba,
Varadero. We are convinced that this meeting will allow creating new perspectives for the future work and it will
consolidate even more the scientific activity of all the participants here congregated.
Thank you to all for your participation.
President of Organizing Committee
FAPRONATURA 2009
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INDICE
PROGRAMA GENERAL / GENERAL PROGRAM
RESÚMENES / ABSTRACTS
CONFERENCIAS DE APERTURA O CONFERENCIAS /
OPENING LECTURES OR LECTURES
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JUEVES 4 de Junio / THURSDAY, June 4
OL-01
OL-02
OL-03
OL-04
Natural and Traditional Medicine in Cuba. Antecedents, Current Situation and Future
Perspectives.
Dra. Martha Pérez Viñas
Modulation of Inflammatory Gene Expression by Natural Compounds. /
Prof. Guy Haegeman
Pharmacogenetics of Herbal Medicine: Potential Relevance for Vulnerability to Depression
and Antidepressant Drug Effect. / Prof. Adrian Llerena
A Clinical Center of Natural Medicine in a Public Hospital: 12 Years of Experience. / Prof.
Fabio Firenzuoli
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30
31
VIERNES, 5 de junio / FRIDAY, June 5
L-05
L-06
L-07
L-08
L-09
L-10
L-11
Cognition-Enhancing Effects of Ginkgo and Ginseng. / Prof. Andrew Scholey
Human Clinical Trials with the Indian Super Medicine Bacopa monniera (BRAHMI). /
Prof. Con Stough.
Memory Enhancing Effect of Bacopa monniera. / Prof. Hemant Singh
Phytotherapeuticals for Behavioural and Psychological Symptoms of Dementia. /
Dr. Ed Okello.
Panax Ginseng (G115) improves aspects of working memory performance, mood in
healthy young adults. / Dr. Jonathon Lee Reay
Presentación del Libro: Los retos de la terapia antioxidante. Colectivo de Autores. /
Alberto Núñez-Sellés, René Delgado, Mariela Guevara, Idania Rodeiro, Dagmar García, Gilberto
Pardo, Beatriz Garrido y otros.)
Presentación del Libro: PLANTAS MEDICINALES. Guía para su uso en la Atención Primaria de
Salud. / MsC. Dr. Nelson Melgarejo López, MsC.Dra. Guadalupe Álvarez Bustamante, Ing. Antonio
Alonso Abad.
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SABADO 6 de junio / SATURDAY, June 6
L-12
L-13
L-14
L-15
L-16
L-17
L-18
L-19
Modulator Effects of Vimang, Mangiferin and Gallic Acid on Proliferation, Genes Expression
and Transduction Signal Pathways in MDA-MB 231 Cancer Cell Line. /
Dra. Dagmar Garcia-Rivera
Catechol Containing Polyphenols, Mitotoxics or Mitoprotectors? The Mangiferin Tale. / Dr.
Gilberto L Pardo-Andreu
C-Phycocyanin Derived from Spirulina Platensis Potentiates Alpha-IFN Induction of
Regulatory
T Cells in Multiple Sclerosis. / Dra. Giselle Pentón Rol
Natural Products: Challenges to Access the Chemical Diversity and New Therapeutic
Targets. / Dra. Evangelina Marrero Faz
Regulatory Framework for the Development of Natural Products. / MSc. Julián Rodríguez
Update in preclinical and clinical regulatory requirements in Cuba. / Dra. Diadelis Remirez
Figueredo
Quality Management System for Clinical Trials with Natural Products in Cuba. /
MSc. Sandra Álvarez Guerra
Flordis Natural Medicines / Dr. Andrea Zangara
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41
DOMINGO, 7 Junio / SUNDAY, June 7
L-20
L-21
Results on pharmacological research to validate traditional medicinal plant uses in TRAMIL
program: 1988-2008. / Dr. Francisco Morón
Natural Products, Since Pre To Postmarketing. / Dra. Giset Jiménez
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L-22
L-23
Development of Clinical Trials in Cuba. Experiences with Natural Products. /
Dra. María Amparo Pascual López
Development of Natural Products in Cuba. Experience of the Drug Research and
Development Center. / MSc. Marlene Porto Verdecia
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COMUNICACIONES ORALES (CO) / ORAL COMMUNICATIONS
OC-01
OC-02
OC-03
OC-04
OC-05
OC-06
OC-07
OC-08
OC-09
OC-10
OC-11
OC-12
OC-13
OC-14
Foods and Drugs Interactions in Pharmaceuticals Products of Natural Origin. /
Dra. Eliza Aznar
Absorption and Metabolism of Flavonoids and Their Metabolites. / MSc. Rodolfo López
Pharmacological Interactions of Traditional Medicine Products Used with Conventional
Drugs. / Dra. Idania Rodeiro-Guerra
Safety of Curcuma longa L in patients assuming warfarin: A serie of 12 patients. /
Dr. Luigi Gori
Physiologically Based Pharmacokinetic Modeling of Theophylline/Coffee Interaction
in Human. / Prof. Francis C. P. Law
Herb-Drug Interactions?. Drug Company Hysteria or Community Concern? /
Prof. Con Stough
Molecular Modeling of Carvacrol (Constituent of Aromatic Plants) by Means of Molecular
Dynamics Simulation / Dra. Maria Cristina Donnamaria
Computational Discovery of New Anthelmintic Compounds from Agave Brittoniana Trel. Spp.
Brachypus. / MSc. Yeniel González-Castañeda
Discovery of Novel Tyrosinase Inhibitors as Promissory Depigment Drugs From Natural
Sources Integrating Theoretical and Experimental Methods. / Dr. Huong Le
Thi Thu
Pharmaceutical Information Center: Its Incidence as a Provider of Specialized Information
Resources. / MSc. Ricardo Castro Armas
Scorpion venom as a Natural medicine. It’s use in medicine. / Dr. José Ramón Rodríguez
Alonso
Pre-Clinical Studies in Animals and in Vitro to Show the Activity of the Rhopalurus junceus
Scorpion Venom in Some Cell Lines of Cancer. Ethnomedical Studies About the Use of
Escozul. / Dr. Sinoel LLorentes Sánchez
Tityus serrulatus Scorpion Venom: Toxins, Mechanisms of Action and Possible medicine
applications. / Dr. Claudio Maurício Vieira de Souza
El Escorpin® CNH, Un Complemento Natural Beneficioso en el tratamiento del cáncer de
próstata. / Dr. Ana Maria Armendáriz Enrique
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45
46
46
47
48
48
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49
OC-15
OC-16
OC-17
OC-18
OC-19
OC-20
OC-21
OC-22
OC-23
OC-24
OC-25
The Use of Natural Products in Chronic Liver Disease and Herbal Hepatotoxicity. /
Dr. Zeki Yesilova
Natural Products as Alternative Treatments for Metabolic Bone Disorders. /
Dr. Cagatay Oktenli
Antithrombotic Potential of Medicinal Plants. / Dra. Milagros García Mesa
Developing Anti-Inflammatory Pharmaceutical Grade Botanical Drugs: Experience
with Organic Sourced PUR 0110. / Dr. André P. Boulet
A Randomized Controlled Trial of VIUSID in Combination to Diet and Exercise in Patients
with Non Alcoholic Steatohepatitis. / Dr. Eduardo Vilar Gómez
Influence of the Nutritional Supplement Diamel on Indexes HOMA - B and HOMA-IR
in Patients with Type 2 Diabetes Mellitus. / Dr. José A. Hernández-Yero
Utilization of Marine Bacteria for Production of Natural Products. / Dr. Roberto Núñez
Pharmacological Characterization of Bioactive Compounds from the Sea Anemone Epicystis
crucifer. / Dra. Anoland Garateix
Antioxidant and Citoprotective Effects of BM-21, an Aqueous Ethanol Extract from Thalassia
testudinum in Various Experimental Models. / Dr. Roberto Menéndez Soto del Valle
New Compounds Obtained from Sponges of Cuban Coasts with Pharmacological Activity. /
Dr. Abilio Laguna
An Investigation of Potential Uses for Amphitoxin, A Pyridinium Alkaloid from the Jamaican
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OC-26
OC-27
OC-28
OC-29
OC-30
OC-31
OC-32
Sponge, Amphimedon Compressa. / Dr. Winklet A. Gallimore
Useful Antitumor Marine Compounds. / Dr. Sudeep Bhardwaj
Impact on Cuba neonatal mortality of use of a natural Surfactant “Surfacen”. Future new
indications. / Dr. Octavio Fernández-Limia
Iron Bioavailability in the Product Line of Natural Origin Trofin: Use of the Radioisotópica
Double Technique. / Dr. Raúl González Hernández
Antiviral Activity of Phyllanthus orbicularis HBK Against Enteroviruses. / Lic. Maikel Acosta
Zaldívar
New Flavonol with Anti-Inflammatory Activity from Boldoa purpurascens, CAV. /
Dra. Dulce María González Mosquera
New Approach of Rhizophora mangle L. Efficacy in Experimental Peptic Ulcers. /
DrC. Luz María Sánchez Perera
Cuban Extract From Stem Bark Of Mangifera Indica L (Vimang): From Preclinical
Studies To The Best Clinical Investigation Strategy: The Permanent Challenge For
The Pharmacology Of Natural Products.. / Dr. René Delgado-Hernández
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58
58
59
60
OC-33
OC-34
OC-35
OC-36
OC-37
OC-38
OC-39
OC-40
OC-41
OC-42
OC-43
OC-44
OC-45
OC-46
OC-47
Regulatory T Cells Induction as a Mechanism of Action for Natural Products in Autoimmune
and Chronic Inflammatory Diseases. / Dra. Majel Cervantes
PKC Activation by Phorbol Esters Isolated from Jatropha curcas / Dra. Chryssa Dimaxi
Role of bioactive flavonoids against oxidative stress in experimental reflux oesophagitis. /
Prof. Ch.V. Rao
Interference of selected flavonoids with the growth of filamentous fungus R. nigricans. / Dr.
Helena Lenasi
Antimicrobial Activity of Tamarindus indica L. Leaves Extracts and their Correlation
with Polyphenol and Flavonoid Composition. / Dr. Julio César Escalona Arranz
Analgesic profile of water extract of stem bark of Mangifera indica L (Vimang®) in patients
with chronic pain. / Dra. Bárbara Beatriz Garrido Suárez
Lipid-Lowering Therapy comparison with Atorvastatina and policosanol on the redox status
in dyslipidemics with peripheral vascular diseases. / Dr. Samuel Fleitas Estévez
Efficacy of Migraprecol in the Prevention of Migraine. Results of a Fase III Clinical Trial. / Lic.
Yisel Avila Albuerne
VIUSID, a Nutritional Supplement, in Patients with Chronic Hepatitis C and Cirrhosis. A
Randomized and Double Blind- Controlled Study. / Dr. Eduardo Vilar Gómez
Clinical Studies Considerations of all Botanical Products. / Dra. Maria Acelia Marrero Miragaya
Magic Bullets and Poison Arrows: The Role of Natural Medicines in Treating Neurocognitive
Decline and Neurodegeneration. / Prof. Andrew Scholey
Linaloe Essential Oil: Its Therapeutic Reincorporation. / Lic. Eva Obregón Domínguez
Aphrodisiac Activity Of Curculigo Orchiodes Gaertn. / Dr. Nagendra Singh Chauhan
The Preclinical and Safety Evaluation of a Traditional Medicinal Product, Phela, A Potential
Immune Modulator. / Dr. Motlalepula G. Matsabisa
The Role of Scientific Evidence on the Attitudes of Herbal Medicine Consumers of Diadema
(São Paulo, Southeastern Brazil). / Dr. Juliana Lanini Mariano
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CARTELES (P) / POSTERS (P)
JUEVES 4 de Junio / THURSDAY, June 4 - VIERNES, 5 de junio / FRIDAY, June 5
POSTERS PRECLINICAL PHARMACOLOGY (PPP-01 – PPP-39)
PPP-01
PPP-02
PPP-03
CYTOTOXIC ACTIVITIES OF PEROXIDASES AND HYPOHALOUS ACIDS. PROTECTION BY
POLYPHENOLS AND OTHER NATURAL COMPOUNDS. / Ronca F., Tagliazucchi D., Maltinti S.,
Gondres Amaro Y., Ronca G.
BIOLOGICAL ACTIVITY OF MELANOIDINS FROM TRADITIONAL BALSAMIC VINEGAR OF
MODENA AND REGGIO EMILIA. / Tagliazucchi D., Verzelloni E., Gondres Amaro Y., Conte A.
INFLUENCE OF GRAPE VARIETY ON NEUROPROTECTIVE AND VASODILATORY EFFECT OF
RED WINES. / Martín S., Gómez-Serranillos P., Ortega T., Palomino O., Carretero E., Prodanov M.,
Vacas V., Cabellos M., Estrella I., Hernández T.
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PPP-04
PPP-05
PPP-06
PPP-07
PPP-08
PPP-09
PPP-10
PPP-11
PPP-12
PPP-13
PPP-14
PPP-15
PPP-16
PPP-17
PPP-18
PPP-19
PPP-20
PPP-21
PPP-22
PPP-23
PPP-24
PPP-25
PPP-26
PPP-27
ESTUDIO DEL POTENCIAL CITOTÓXICO DEL GLICOLIPIDO TETRASACARIDO STANCINA IV,
DE Ipomea stans, SOBRE LINEAS CELULARES. / Elizalde, J., León, I y González-Christen, J
CONTENIDO DE POLIFENOLES TOTALES Y CAPACIDAD ANTIOXIDANTE EN EXTRACTOS
ACUOSOS Y ETANÓLICOS DE HOJAS Y CORTEZA DE Ochroma pyramidale (Cav. ex. Lam). /
Tello W, Guerrero T, Sandoval M
ANTIAGING ACTION OF MELATONIN / Prieto-Gómez B., Santibáñez-Olvera C., Moreno-López Y.
L., Reyes-Prieto N. M. y Velázquez-Paniagua M.
MELATONIN REGULATES FERTILITY AND SEXUAL BEHAVIOR OF MALE RATS. / VelázquezPaniagua M., Parra-Gámez L., Gutiérrez-Ruiz J., García-Hidalgo A., Balderas-López Eduardo J.,
and Prieto-Gómez B.
METABOLIC STUDIES ON THE ANTISPERMATOGENIC EFFECT OF CARICA PAPAYA LINN.
SEED EXTRACTION. / DrC. CHANGAMMA
PROTECTIVE EFECT OF Bidens pilosa L EXTRACT IN HEPATOTOXICITY INDUCED BY
PARACETAMOL. / Rayza Méndez Triana MSc, María BoffIill Cárdenas PhD, Emilio E. Monteagudo
Jiménez MSc, Maykel Pérez Machín MSc, Omaida López Bernal MD.
PRECLINICAL EVALUATION OF THE ANTI-INFLAMMATORY ACTIVITY OF WHITE BIDENS
(ROMERILLO). / Pajarín F L, Álvarez G RM.
ANTI-INFLAMMATORY, ANTIOXIDANT AND ANALGESIC EVALUATION ON Murraya paniculata. /
Casado C., Gutiérrez Y., García G., López R., Miranda M.
ANTI-INFLAMMATORY ACTIVITY OF TWO FLAVONOLIC EXTRACTS OF THE PETIVERIA
ALLIACEA L. IN RATS. / Gross Fernández MC.
ANTI-INFLAMMATORY AND CYTOTOXIC ACTION OF THE THYME (THYMUS VULGARIS). /
Ferrándiz D., Iglesias E., Valls AR., Pérez PL.
EVALUATION IN VIVO OF A FLAVONOID OF PIPER AURITUM IN A MODEL OF RETARDED
HYPERSENSITIVITY. / Pérez PL, Ferrándiz D, Iglesias E, Valls AR.
PROTECTIVE EFFECT OF MANGIFERA INDICA L. EXTRACT ON DEXTRAN SULFATE
SODIUM- INDUCED COLITIS IN RATS. / Márquez L, Garrido G, Gómez B, Garate I, Muñoz JL,
Menchén L, Lesa Cerro JC
EFFECT OF POLICOSANOL FROM SUGAR CANE ON INFLAMMATORY AND WOUND HEALING
IN EXPERIMENTAL MODELS. / Boffill M, Monteagudo E, Martínez Y, Villanueva G, Quesada D.
EVALUATION OF THE DIURETIC ACTIVITY OF SIX PLANTS USED BY THE CUBAN
POPULATION. / Pérez M, Tamayo M, Boffill M, Lorenzo G, Jiménez E, Betancourt E, Iglesias N,
Verdecía B, Díaz L.
IN VITRO EFFECT OF TROFIN®, AN HYDROLYZED OF PROTEINS AND HEME IRON MIXTURE
ON IONIC IRON REDOX STATUS. / García Y, Martínez D, González R, Pardo-Andreu GL
EFFECTS OF NATURAL ZEOLITE MODIFIED WITH ZINC (ZZ) ON STREPTOZOTOCIN-INDUCED
DIABETES MODEL IN RATS. / Torres A, León O, Casanova M, Rodríguez G.
POLYPHENOL COMPOUNDS & PHARMACOLOGICAL REDOX MECHANISMS INVOLVED IN
WOUND HEALING. RHIZOPHORA MANGLE CASE STUDY. / Janet Sánchez, Roberto Faure,
Gregorio Martínez, Octavio Fernández, Ernesto Vega and Evangelina Marrero.
UPDATE OF EXPERIMENTAL BIOPHYSICAL AND RHEOLOGY PROPERTIES OF NATURALS
LUNG SURFACTANTS. / Odalys B., Cruz A, Morales I, Faure R1 and Pérez-Gil J
IN VITRO AND IN VIVO ANTI-TUMOUR EFFECT OF THE AQUEOUS EXTRACT OF THE
MANGIFERA INDICA L. STEM BARK EXTRACT AND ITS MAIN COMPONENT MANGIFERIN. /
Magariño Y, López O, Coipel L and Delgado R.
EVALUATION IN VITRO OF THE RADIOPROTECTIVE EFFECT OF THE AQUEOUS EXTRACT
OF MANGIFERA INDICA L. (VIMANG). / Rosario L, Rodeiro I, Almeida E, Leyva O, Alonso A,
Delgado R, Rodriguez Y.
PROTECTIVE EFFECT OF THE Phyllanthus orbicularis, HBK AQUEOUS EXTRACT ON HUMAN
CELLS AGAINST THE DAMAGE INDUCED BY UVB RADIATION. / Vernhes M, GonzálezPumariega M, Andrade L, Passaglia A, Moreno de Lima-Bessa K, Menck CF and Sánchez-Lamar
A.
THE PROTECTIVE CAPACITY OF Cymbopogon citratus (DC) Staff EXTRACT AGAINST THE
DAMAGE INDUCED BY ULTRAVIOLET RADIATION / González-Púmariega, M, Vernhes, M,
Menck CF, Sánchez-Lamar, A.
ANTIPROTOZOAL BIOMONITORED FRACTIONATION OF THE BRAZILIAN PLANTS EXTRACTS
HIMATANTHUS SUCUUBA AND CAMPSIANDRA COMOSA. / Duarte-Almeida JM, Tempone AG,
Reimão JQ, Rodrigues E, Carlini EA.
SEARCH FOR NEW SECONDARY METABOLITES WITH BIOLOGICAL ACTIVITY FROM
10
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71
72
72
73
74
74
75
75
76
76
77
77
78
78
79
79
80
80
81
81
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PPP-28
PPP-29
PPP-30
PPP-31
PPP-32
PPP-33
PPP-34
PPP-35
PPP-36
PPP-37
PPP-38
PPP-39
ENDOPHYTIC FUNGI. / Hidalgo PI, Anke T, Meffert A, Medina RP, Peñate L, García MR,
Casanova M, Enríquez V
ANTILEISHMANIAL ACTIVITY OF BIXA ORELLANA. / García M, Monzote L, Montalvo AM, Scull
R.
CHEMISTRY COMPOSITION, CITOTOXICITY AND ANTILEISHMANIAL ACTIVITY OF THE
ESSENTIAL OIL FROM PIPER AURITUM. / Monzote L, García M, Scull R, Miranda M.
EFFECT OF THE SEASONAL VARIABILITY IN THE COMPOSITION AND THE BIOLOGICAL
ACTIVITY OF THE ESSENTIAL OIL OF SIPARUNA BIFIDA. / Saavedra G, Poka D, Ruiz G.
SEARCH FOR ANTIVIRAL ACTIVITY OF THE PLANT AGERATINA HAVANENSIS AGAINST DNA
AND RNA VIRUSES. / Acosta M, Álvarez AL, Sarmiento L, García T, Acosta I, Spengler I, Roque A,
del Barrio G.
AGERATINA HAVANENSIS HBK EXTRACTS INHIBIT RABBIT VESIVIRUS IN VITRO
REPLICATION. / del Barrio G, Álvarez A.L, Acosta M, Spengler I, García, T y F. Parra.
PHYLLANTHUS ORBICULARIS INHIBITORY ACTIVITY AGAINST HERPES SIMPLEX VIRUSES
AND HUMAN CYTOMEGALOVIRUS: AN APPROACH TO ANTIVIRAL MECHANISMS. / Álvarez
AL, Kourí V, Martínez PA, Parra F, del Barrio G.
IN VITRO ANTIVIRAL ACTIVITY OF MEDICINAL PLANTS AGAINST HEPATITIS B VIRUS (HBV). /
Roque A, González R, Morier L, Rodríguez LA, del Barrio G.
THE COEFFICIENT OF SPECIFICITY OF INHIBITION AgsVBH IN SPECIES CUBAN
PHYLLANTHUS. / Chevalier P, León M, Hernández M, Cedeño P, Vidal M.
ANTIBACATERIAL ACTIVITY OF FOUR NATURAL PRODUCTS. / Echemendía O, Almora E,
Fidalgo O, Monteagudo R, Bolaños G, Lago V.
ANTIMICROBIAL EVALUATION OF TOTAL EXTRACTS FROM LEAVES OF PETIVERIA
ALLIACEA L. (ANAMÚ). / Ochoa AP, Marín JM, Mujawimana RJ, Tamayo KRG, González ZG.
CITOTOXICITY OF FRACTIONS FROM OF THE ESPECIE ERYTHROXYLUM MINOTIFOLIUM
ON TWO CELLS LINES. / Almora E, Monteagudo R, Fidalgo O, Echemendía O, Bolaños G,
García M, Lago V, González JA.
INCREASED INSULIN SECRETION IN RAT BETA CELLS TREATED WITH LOW MOLECULAR
WEIGHT FRACTIONS FROM Physalia physalis. / Díaz García CM, Sánchez Soto C, Fuentes D,
Rodríguez A, Pedroso A, Hiriart Urdanivia M, Castañeda Pasarón O.
83
84
84
85
85
86
87
87
88
88
89
89
POSTERS CLINICAL PHARMACOLOGY (PCP-01 – PCP-08)
PCP-01
PCP-02
PCP-03
PCP-04
PCP-05
PCP-06
PCP-07
PCP-08
FERMENTED RICE IN PATIENTS SUFFERING ADVERSE REACTION TO SYNTHETIC HMGCoA REDUCTASE INHIBITORS: TREAMENT OF A SERIES OF 24 PATIENTS WITH
FERMENTED RED RICE. / Gori L, Mascherini V, Firenzuoli F
ANTI-INFLAMMATORY EFFECT OF AN EXTRACT OF MANGIFERA INDICA L. (VIMANG®) IN
PATIENTS WITH CHRONIC DERMATITIS. / Guevara M, Pérez T, Calderón B, Perdomo J, Morales
C, Garrido G.
EFFECTS OF SURFACEN IN LOW AND MAINTAINED DOSE IN THE TREATMENT OF ARDS.
PRELIMINARY RESULTS. / Barrese Y, Avila Y, Díaz E y Fernández-Limia O
LEPIDIUM MEYENI (MACA). IT’S EFFECTS ON SEXUAL DESIRE AND SERUM REPRODUCTIVE
HORMONE LEVELS IN A GROUP OF MALES OVER 50 YEARS OF AGE. / Valdés Carrillo R,
Trasanco Delgado M, Becerra N, Tur Behar R.
EVALUATION OF EFFICACY AND SECURITY OF DERMOFURAL OINTMENT IN PATIENTS
WITH SQUAMOSA AND CORPORIS TINEA PEDIS. / Ruiz Calabuch H, Jiménez J, Silva J, Ramos
L, Leon O, Betancourt J.
USE AND APPLICATION OF GARLIC (ALLIUM SATIVUM L) IN VIRAL AFFECTIONS ON THE
SKIN. / Marin N, López K, Sánchez to, Vargas R.
THE SAFETY AND EFFICENCY OF 20% HERBAL GUACAMAYA FRANCESA IN THE
TREATMENT OF INTERDIGITAL EPIDERMOFOSIS INFECTION. / Galbán M, Medina A, Pérez M,
Moreia A, Liste S, Artiles M, Santos N, Suero B, Zaita Y, Martínez B, Bauta R, Orozco M.
EFFECTIVENESS AND SEGURITY OF THE DYE OF PEDILANTHUS TITHYMALOIDES (L) POIT.
(ITAMO REAL) IN THE TREATMENT OF EDEMATOUS CHRONIC GINGIVITIS. / Ortiz Y,
Martínez H, López T, Cabrera Z.
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90
91
91
92
92
93
93
POSTERS ETHOPHARMACOLOGICALS AND USES STUDIES (PEP-01 – PEP-03)
94
PEP-01
MEDICINAL PLANTS UTILIZED IN RURAL COMMUNITIES OF THE ANDEAN PIEDMONT,
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FAPRONATURA 2009
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PEP-02
PEP-03
VENEZUELA: Traditional uses and relative importance. / Bermúdez A., Oliveira M., Carrillo T.
UTILIZATION OF MEDICINAL PLANTS IN AN URBAN COMMUNITY OF THE TRUJILLO STATE,
VENEZUELA: TRADITIONAL USES, RELATIVE IMPORTANCE AND PATTERN OF
DISTRIBUTION OF THE TRADITIONAL KNOWLEDGE. / Carrillo T., Bermúdez A., Oliveira M.
A PHARMACOECONOMIC STUDY COMPARING A NATIONAL NATURAL TOPIC PRODUCT
WITH A FOREIGN TOPICAL CREAM. / Alfonso K., Collazo M., Fernández M., Ballagas C.
94
95
POSTERS TOXICOLOGICALS STUDIES (PTS-01 – PTS-06)
96
PTS-01
PTS-02
PTS-03
PTS-04
PTS-05
PTS-06
HYPOGLYCEMIC ACTIVITY AND ACUTE TOXICITY OF FOUR PLATS POPULARLY USED IN
CUBA. / Monteagudo E, Boffill M, Betancourt E, Quesada D, Blanco F, Díaz L, Verdecía B, Iglesias
N.
TOXICOLOGICAL EVALUATION OF POWDER OF DE PARTHENIUM HYSTEROPHORUS, L;
USING ALTERNATIVES TWO TECHNIQUES. / Saucedo Y, García D, Tenorio E, Monteagudo E.
TOXICITY STUDY TO A REPEATED DOSE OF THE AZADIRACHTA INDICA A. JUSS PLANT
(NIM´S TREE). / Berenguer CA, Alfonso A, Fong O, Domínguez A, Betancourt JE, Laramendi D,
Salas H, Puentes E, Wawoe N, Cedeño N.
GENOTOXIC EVALUATION IN VITRO OF ERYTHROXYLUM MINUTIFOLIUM, AN ENDEMIC
CUBAN PLANT. / Leyva O, Alonso A, Rodeiro I, Díaz A, Rosario L, Carro S, Gonzalez-Lavaut A
ASSESSMENT OF THE POTENTIAL GENOTOXIC EFFECT OF AQUEOUS EXTRACTS FROM
BRYOTHAMNION TRIQUETRUM, HALIMEDA INCRASSATTA AND HALIMEDA MONILE
SEAWEEDS, USING SOS CHROMOTEST ASSAY. / Sánchez-Lamar A, Cuevas A, Alonso A,
Almeida E, Verhnes M.
TOXICOLOGICAL AND PHARMACOLOGICAL PROPERTIES OF A LOW MOLECULAR WEIGHT
FRACTION FROM ZOANTHUS SOCIATUS (CNIDARIA) ASSESSED IN OF-1 MICE. / Domínguez
Pérez D, Díaz García CM, Pérez Saad H, García Delgado N, Varela C, Sierra Y, Fuentes D, Acela
Pedroso, Rodríguez A, Hiriart M, Castañeda Pasarón O.
96
97
97
98
98
POSTERS ALTERNATIVE MEDICINES (PAM-01 – PAM-07)
PAM-01
PAM-02
PAM-03
PAM-04
PAM-05
PAM-06
PAM-07
USE OF CHELLIDONIUM® (A HOMEOPATHIC MEDICINE) FOR THE TREATMENT OF ICTERUS
IN CATS. / Zafar MA, Mahfooz A, Abbas RZ, Sohail M and Manzoor A
APPLICATION OF THE MAGNETIC INDUCTIVE STABILIZER (EIMA®) AND OZONETHERAPY IN
THE TREATMENT OF PATIENTS WITH DISC HERNIA. / Yepes A, Aguilera E, Gómez R, Martínez
I, Aguilar B, Kindelán L.
ACUPOINT INJECTION WITH MINIMAL DOSES OF STEROIDS FOR JOINT PAIN RESISTANT. /
Perdomo Delgado J, González Pla E, Castro Morillo E, Páez Casona T, Sánchez Hernández T.
HEMOSTATIC EFFECT OF ULTRA HIGH DILUTIONS OF PHOSPHORUS ON SURGICAL
PATIENTS. / Consuegra Leon L, Perdomo Delgado J, González Pla E, Trimiño Galindo L, Otero
Barroso M.
SURGICAL ACUPUNTAURAL ANALGESIA OF CATARACT IN GERIATRICS PATIENT. /
Domínguez González E.J.
EFFICACY OF RESCUE REMEDY IN THE TREATMENT OF INTERMITENT MODERATE
ASTHMA CRISIS. / Ferrada Zapatero D, Rodríguez Gutiérrez M, Pupo Machado M, Vila Rodríguez
I, León Ramírez M, Ávila Pérez Y, Labrada Vila I.
EFFECTIVENESS OF THE USE OF THE SAMBUCUS IN THE TREATMENT OF THE
BRONCHIAL HIPERREACTIVITY IN PEDIATRIC AGE. / Herrero Aguirre H, Álvarez G. RM.
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100
100
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POSTERS PHARMACEUTICALS TECHNOLOGIES (PPT-01 – PPT-02)
PPT-01
PPT-02
SOLAR DRYING CURVES FOR THREE MEDICINAL SPECIES. CURVAS DE SECADO SOLAR
PARA TRES ESPECIES MEDICINALES. / JEREZ A, CHINAPPI I, UZCATEGUI M.
METHOD OF EXTRACTION CARVONE BY DIRECT INDUCTION HEATING – ASSISTED
MAGNETIC FIELD. / L. Lagunez Rivera, G. Vilarem.
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PROGRAMA GENERAL / GENERAL PROGRAM
MIÉRCOLES 3 de Junio / WEDNESDAY, June 3
Salida Delegados extranjeros del Hotel Occidental Miramar, Ciudad de La
Habana hacia Hotel Oasis Varadero, Playa de Varadero, Matanzas, Cuba /
Departure of foreigners delegates from Occidental Miramar Hotel, Havana
to Oasis Varadero Hotel, Varadero Beach, Matanzas, Cuba.
14:00 – 16:00
Recibimiento y Acreditación a Delegados extranjeros. Ubicación en
Habitaciones del Hotel Oasis Varadero / Welcome and Accreditation to
Foreigners Delegates. Location in Rooms at Oasis Varadero Hotel.
19:00-21:00
Cena de Bienvenida para delegados extranjeros hospedados en el Hotel
“Oasis Varadero 1920” / Dinner to Foreigners Delegates housed at “Oasis
Varadero 1920” Hotel.
21:30
Actividad Cultural de Bienvenida / Welcome Cultural Activity.
12:00
Sala de Conferencias /
Room of Lectures
Hotel “Oasis Varadero 1920”
15:00-15:20
Opening
Lecture 01
15:25-16:10
Opening
Lecture 02
16:15-17:00
Opening
Lecture 03
17:05-17:50
Opening
Lecture 04
17:55-18:40
19:00-20:00
20:30-23:30
Inauguración del Simposio y Conferencias de Apertura / Inauguration
of Symposium and Opening Lectures (OL)
PALABRAS DE APERTURA / Opening words
Dr. René Delgado-Hernández. Presidente de la Sociedad Cubana de
Farmacología / President, Cuban Society of Pharmacology
MEDICINA NATURAL Y TRADICIONAL EN CUBA. ANTECEDENTES,
SITUACIÓN ACTUAL Y PERSPECTIVAS FUTURAS. / Natural and
Traditional Medicine in Cuba. Antecedents, current situation and future
perspectives.
Dra. Martha Pérez Viñas. Directora del Centro Nacional de Medicina
Natural y Tradicional de Cuba. / Director, National Center for Natural and
Traditional Medicine, Cuba.
MODULACIÓN DE LA EXPRESIÓN DEL GENES INFLAMATORIOS POR
COMPUESTOS NATURALES. / Modulation of Inflammatory Gene
Expression by Natural Compounds.
Prof. Guy Haegeman. Director, Laboratory of LEGEST, University of Gent,
Belgium. / Bélgica.
FARMACOGENETICA DE MEDICINA HERBARIA: RELEVANCIA
POTENCIAL DE LA VULNERAVILIDAD A LA DEPRESION Y AL EFECTO
DE LOS ANTIDEPRESIVOS / Pharmacogenetics of Herbal Medicine:
Potential Relevance for Vulnerability to Depression and Antidepressant Drug
Effect.
Prof. Adrián Llerena, Director CICAB. Centro de Investigación Clínica.
Hospital Universitario. Universidad de Extremadura, Badajoz, España.
Coordinador de la Red Iberoamericana de Farmacogenética CYTED.
UN CENTRO CLÍNICO DE MEDICINA NATURAL EN UN HOSPITAL
PÚBLICO: 12 AÑOS DE EXPERIENCIA. / A Clinical Center of Natural
Medicine in a Public Hospital: 12 Years of Experience.
Prof. Fabio Firenzuoli. Director, Clinical Center of Natural Medicine S.
Giuseppe Hospital, Az USL 11 Empoli, Italy. / Italia.
CENA / DINNER
COCTEL DE BIENVENIDA Y SECCIÓN DE CARTELES. Inauguración de
Feria Exposición sobre Productos Naturales / WELCOME RECEPTION
AND SECTION OF POSTERS. Inauguration of Exhibition Fair of Natural
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FAPRONATURA 2009
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Products.
Coordinadores /
Coordinators:
Dres. Mariela Guevara, Beatriz Garrido, Roberto Menéndez, Odalys
Blanco, Lianne Pajarin, Doris Perdomo, Hector Ruiz Calabuch, Maria
Bofill, Cuba.
20:30-21:30
DISCUSIÓN DE CARTELES (CARTEL PPP-01 – PPP-39)
FARMACOLOGÍA PRECLINICA E INTERCAMBIO CIENTÍFICO.
PRESENTACIÓN ORAL (5 MIN) DE CARTELES SELECCIONADOS /
Poster discusión (POSTERS PPP-01 – PPP-39) PRECLINICAL
PHARMACOLOGY and Scientific Exchange. Oral Presentation (5 min) of
Selected Posters.
21:30-23:30
Degustación de Rones Cubanos. Música y Karaoke / Tasting of Cuban
Rums. Music and Karaoke.
Room of Lectures
”Hotel Oasis Varadero 1920”
Coordinador / Coordinator:
Lecture 05
09:00-09:25
Lecture 06
09:30-09:55
Lecture 07
10:00-10:25
Lecture 08
10:25-10:50
Lecture 09
10:55-11:20
TALLER, EMPRESA FLORDIS, AUSTRALIA. “EL USO DE LOS
EXTRACTOS HERBARIOS PARA EL PERFECCIONAMIENTO
NEUROCOGNITIVO”. / ''FLORDIS KEENMIND WORKSHOP”. “THE
USE
OF
HERBAL
EXTRACTS
FOR
NEUROCOGNITIVE
ENHANCEMENT”.
Dr. Andrea Zangara
Flordis Natural Medicines - International Business Development Manager,
Australia.
EFECTOS ESTIMULADORES DE LA FUNCION COGNITIVA DEL
GINKGO Y EL GINSENG. / Cognition-Enhancing Effects of Ginkgo and
Ginseng.
Prof. Andrew Scholey. Herbal and Nutritional Medicine Research Unit.
Brain Sciences Institute, Swinburne University, Australia.
ENSAYOS CLINICOS CON LA SUPERMEDICINA INDIA BACOPA
MONNIERA (BRAHMI). / Human Clinical Trials with the Indian Super
Medicine Bacopa Monniera (BRAHMI).
Prof. Con Stough. Herbal and Nutritional Medicine Research Unit. Brain
Sciences Institute, Swinburne University, Australia.
EFECTO ESTIMULADOR DE LA MEMORIA DE BACOPA MONNIERA. /
Memory Enhancing Effect of Bacopa monniera.
Prof. Hemant Singh. Ex-Deputy Director, C.D.R.I, Director, Hygia Institute
of Pharmaceutical Education and Research, Lucknow, India.
PRODUCTOS FITOFARMACÉUTICOS PARA LOS SÍNTOMAS
CONDUCTUALES Y PSICOLÓGICOS ASOCIADOS A LA DEMENCIA. /
Phytotherapeuticals for behavioural and psychological symptoms of
dementia.
Dr. Ed Okello. Executive Director of the Medicinal Plant Research Group,
School of Agriculture, Food and Rural Development. Agriculture Building,
Newcastle University, Newcastle upon Tyne, United Kingdom. / Reino
Unido
PANAX GINSENG (G115) MEJORA ASPECTOS ASOCIADOS AL
DESARROLLO DE LA MEMORIA DE TRABAJO Y EL HUMOR EN
ADULTOS JÓVENES SALUDABLES. / Panax Ginseng (G115) improves
aspects of working memory performance, mood in healthy young adults.
Dr. Jonathon Lee Reay
Brain Performance and Nutrition Research Centre, Northumbria
University, Newcastle upon Tyne, UK.
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FAPRONATURA 2009
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11:25-11:45
Salas / Rooms
1,2,3
SALA 1 /
ROOM 1
11:50-14:30
11:50-14:00
Coordinadores / Coordinators:
RECESO / COFFEE BREAK
COMUNICACIONES ORALES / ORAL COMMUNICATIONS
SIMPOSIOS / SYMPOSIA
COMUNICACIONES ORALES (CO) / ORAL COMMUNICATIONS (OC)
Simposio: INTERACCIONES FARMACOLÓGICAS DE PRODUCTOS
NATURALES / Pharmacological interactions of natural products.
Dra. Idania Rodeiro, Cuba & Dr. Adrian Llerena, España.
14:15-14:25
INTERACCION ALIMENTOS-MEDICAMENTOS
EN
PRODUCTOS
FARMACEUTICOS DE ORIGEN NATURAL. / Foods and Drugs
Interactions in Pharmaceuticals Products of Natural Origin.
Dra. Eliza Aznar (PhD). BIOCEN, Cuba.
ABSORCION Y METABOLISMO DE FLAVONOIDES Y SUS
METABOLITOS. / Absorption and Metabolism of Flavonoids and Their
Metabolites.
MSc. Rodolfo López, Pharmacy and Foods Institute. University of
Havana, Cuba.
INTERACCIONES FARMACOLOGICAS DE PRODUCTOS DE LA
MEDICINA TRADICIONAL CON FARMACOS CONVENCIONALES. /
Pharmacological Interactions of Traditional Medicine Products Used with
Conventional Drugs.
Dra. Idania Rodeiro-Guerra, Departamento de Farmacología, CEBIMAR,
Cuba.
SEGURIDAD DE CURCUMA LONGA L EN PACIENTES QUE
CONSUMEN WALFARINA: UNA ESTUDIO DE 12 PACIENTES. / Safety
of Curcuma longa L in patients assuming warfarin: A serie of 12
patients.
Dr. Luigi Gori. Clinical Center of Natural Medicine, S. Giuseppe Hospital,
Empoli, Italy. / Italia.
MODELACIÓN FARMACOCINÉTICA BASADA EN FISIOLOGIA DE
LAS INTERACCIONES TEOFILINA/CAFÉ EN HUMANOS. /
Physiologically Based Pharmacokinetic Modeling of Theophylline/Coffee
Interaction in Human.
Prof. Francis C. P. Law. Department of Biological Sciences, Simon
Fraser University, Canada.
¿INTERACCION ENTRE HIERBAS Y DROGAS?. ¿HISTERIA DE LAS
COMPANIAS PRODUCTORAS DE DROGAS O PREOCUPACIÓN DE
LA COMUNIDAD? / Herb-Drug Interactions?. Drug Company Hysteria
or Community Concern?
Prof. Con Stough
National Institute of Complementary Medicine (NICM) Collaborative
Research Centre in Neurocognition and Natural Medicines, Brain Sciences
Institute, Swinburne University, Victoria, 3122, Australia
DISCUSIÓN GENERAL / General Discussion
14:30-15:30
ALMUERZO / LUNCH
OC-01
11:50-12:10
OC-02
12:15-12:35
OC-03
12:40-13:00
OC-04
13:05-13:25
OC-05
13:30-13:50
OC-06
13:55-14:15
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FAPRONATURA 2009
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SALA 2 /
ROOM 2
14:10-14:25
Symposio: BIOINFORMATICA Y DISEÑO MOLECULAR DE NUEVOS
FARMACOS A PARTIR DE FUENTES NATURALES. / Symposium:
Bioinformatic and Molecular Design of New Drugs from Natural
Sources.
Dra. Maria Cristina Donnamaria, Argentina y MSc. Ricardo Castro,
Cuba.
MODELACION MOLECULAR DE CARVACROL (CONSTITUYENTE DE
PLANTAS AROMATICAS) POR MEDIO SIMULACIÓN EMPLEANDO
DINÁMICA MOLECULAR. / Molecular Modeling of Carvacrol (Constituent
of Aromatic Plants) by Means of Molecular Dynamics Simulation.
Dra. Maria Cristina Donnamaria (PhD)
Instituto de Física de Líquidos y Sistemas Biológicos, IFLYSIB (CONICETUNLP-CIC), La Plata, Argentina.
DESCUBRIMIENTO COMPUTACIONAL DE NUEVOS COMPUESTOS
ANTIHELMINTICOS DE AGAVE BRITTONIANA TREL. SPP.
BRACHYPUS. / Computational Discovery of New Anthelmintic
Compounds from Agave Brittoniana Trel. Spp. Brachypus.
MSc. Yeniel González-Castañeda
Unit of Computer-Aided Molecular “Biosilico” Discovery and Bioinformatic
Research (CAMD-BIR Unit), Faculty of Chemistry-Pharmacy. Central
University of Las Villas, Santa Clara, Villa Clara, Cuba.
DESCUBRIMIENTO DE NUEVOS INHIBIDORES DE TIROSINASA
INTEGRANDO METODOS TEORICOS Y EXPERIMENTALES COMO
PROMISORIAS
DROGAS
DESPIGMENTADAS
DE
FUENTES
NATURALES. / Discovery of Novel Tyrosinase Inhibitors as Promissory
Depigment Drugs From Natural Sources Integrating Theoretical and
Experimental Methods.
Dr. Huong Le Thi Thu
Unit of Computer-Aided Molecular ‘‘Biosilico’’ Discovery and Bioinformatic
Research (CAMD-BIR Unit), Faculty of Chemistry-Pharmacy, Central
University of Las Villas, Santa Clara, Villa Clara, Cuba.
CENTRO DE INFORMACIÓN FARMACÉUTICA: SU INCIDENCIA
COMO
PROVEEDOR
DE
RECURSOS
DE
INFORMACIÓN
ESPECIALIZADOS. / Pharmaceutical Information Center: Its Incidence as
a Provider of Specialized Information Resources.
MSc. Ricardo Castro Armas, CIDEM, Cuba.
14:30-15:30
ALMUERZO / LUNCH
11:50-14:30
OC-07
11:50-12:20
OC-08
12:25-12:55
OC-09
13:00-13:30
OC-10
13:35-14:05
SALA 3 /
ROOM 3
11:50-14:00
OC-11
11:50-12:10
Taller / Workshop: I International Workshop about Scorpion Venom
as a Natural Medicine.
Dr. José Ramón Rodriguez Alonso and Dr. Sinoel LLorentes Sánchez
(PhD), Medical University of Guantánamo, Cuba.
LOS VENENOS DE ESCORPIONES COMO PRODUCTO NATURAL. SU
USO EN LA MEDICINA. / Scorpion Venom As a Natural Medicine. It’s
Use in Medicine.
Dr. José Ramón Rodríguez Alonso. Universidad Médica de
Guantánamo (UMG), Guantánamo. Cuba
16
FAPRONATURA 2009
of Natural Products
13:55-14:14:25
ESTUDIOS PRECLINICOS EN MODELOS ANIMALES E IN VITRO QUE
DEMUESTRAN LA ACTIVIDAD DEL VENENO DEL ESCORPION
RHOPALURUS JUNCEUS EN ALGUNAS LINEAS CELULARES DE
CANCER. ESTUDIOS ETHNOMEDICOS SOBRE EL USO DEL
ESCOZUL./ Pre-Clinical Studies in Animals and in Vitro to Show the
Activity of the Rhopalurus junceus Scorpion Venom in Some Cell Lines of
Cancer. Ethnomedical Studies About the Use of Escozul.
Dr. Sinoel LLorentes Sánchez (PhD). Grupo Provincial de Proyectos
Sectorial Provincial de Salud Guantánamo, Cuba.
VENENO DE ESCORPIÓN TITYUS SERRULATUS: TOXINAS,
MECANISMOS DE ACCIÓN Y POSIBLES APLICACIONES EN LA
MEDICINA. / Tityus serrulatus scorpion venom: toxins, mechanisms of
action and possible medicine applications.
Dr. Claudio Maurício Vieira de Souza, INSTITUTO VITAL, NITERÓI,
BRAZIL.
EL ESCORPÍN® CNH, UN COMPLEMENTO NATURAL BENEFICIOSO
EN EL TRATAMIENTO DEL CÁNCER DE PRÓSTATA. / The ESCORPIN
CNH, a natural suplement for the treatment of prostate cancer.
Dr. Ana Maria Armendáriz Enrique. Gerente General. A & A CITIC,
Guadalajara, Jalisco. México.
14:00-15:00
ALMUERZO / LUNCH
15:00-18:30
Actividades Recreativas y Libre Intercambio entre Delegados. / Recreational
Activities and Free Exchange Inter Delegates.
18:30-20:00
CENA / DINNER
20:30-21:30
SECCIÓN DE CARTELES / SECTION OF POSTERS
OC-12
12:15-12:35
OC-13
12:40-13:00
OC-14
13:05-13:25
20:30-21:30
20:30-21:30
Lecture 10
Lecture 11
21:30-23:30
Dres. Mariela Guevara, Beatriz Garrido, Roberto Menéndez, Odalys
Blanco, Lianne Pajarin, Doris Perdomo, Hector Ruiz Calabuch, Maria
Bofill, Cuba.
DISCUSIÓN
DE
CARTELES
(CARTELES
RESTANTES)
FARMACOLOGÍA CLINICA Y OTROS. INTERCAMBIO CIENTÍFICO.
PRESENTACIÓN ORAL (5 MIN) DE CARTELES SELECCIONADOS /
Posters discusión (REMAINING POSTERS) CLINICAL PHARMACOLOGY
AND OTHERS. Scientific Exchange. Oral Presentation (5 min) of Selected
Posters.
PRESENTACIÓN Y COMERCIALIZACIÓN DE LIBROS Y COMPENDIOS
INFORMATIVOS SOBRE PRODUCTOS NATURALES / Presentation and
Commercialization books and Compendium of Natural Products
- Presentación del Libro: Los retos de la terapia antioxidante. Colectivo
de Autores. (Colectivo de Autores. (Alberto Núñez-Sellés, René Delgado,
Mariela Guevara, Idania Rodeiro, Dagmar García, Gilberto Pardo, Beatriz
Garrido y otros.)
- Presentacion del Libro: PLANTAS MEDICINALES. Guía para su uso
en la Atención Primaria de Salud.
MsC. Dr. Nelson Melgarejo López, MsC.Dra. Guadalupe Álvarez
Bustamante, Ing. Antonio Alonso Abad.
Clínica de Medicina Natural de La Habana Vieja, Cuba.
Degustación de Rones Cubanos. Música y Karaoke. / Tasting of Cuban Rums.
Music and Karaoke.
17
FAPRONATURA 2009
of Natural Products
Room of Lectures
”Hotel Oasis Varadero 1920”
Coordinadores /
Coordinators:
Lecture 12
09:00-09:35
Lecture 13
09:40-10:15
Lecture 14
10:20-10:55
Lecture15
11:00-11:35
11:40-11:55
Symposio: Evaluación Farmacológica y Potencialidades Terapéuticas de
Productos Naturales / Symposium: Pharmacological Evaluation and
Therapeutics Potentialities of Natural Products.
Dr. Gilberto L Pardo-Andreu (PhD) and Dr. Evangelina Marrero Faz
(PhD, DrSc)
EFECTOS MODULADORES DEL VIMANG (EXTRACTO ACUOSO DE LA
CORTEZA DE MANGIFERA INDICA L), LA MANGIFERINA Y EL ÁCIDO
GÁLICO SOBRE LA PROLIFERACIÓN CELULAR, EXPRESIÓN DE
GENES Y SEÑALES DE TRANSDUCCIÓN EN LA LINEA CELULAR MDAMB-231. / Modulator Effects of Vimang, Mangiferin and Gallic Acid on
Proliferation, Genes Expression and Transduction Signal Pathways in MDAMB 231 Cancer Cell Line.
Dra. Dagmar Garcia-Rivera (PhD). Center of Biomolecular Chemistry,
Cuba.
POLIFENOLES QUE CONTIENEN CATECOLES; MITOTOXICOS O
MITOPROTECTORES?: LA HISTORIA DE LA MANGIFERIN. / Catechol
Containing Polyphenols, Mitotoxics or Mitoprotectors? The Mangiferin Tale.
Dr. Gilberto L Pardo-Andreu (PhD). IFAL, Havana University, Cuba.
C-FICOCIANINA DERIVADA DE SPIRULINA PLATENSIS POTENCIA
INDUCCION DE CELULAS T REGULADORAS POR ALFA INTERFERON
EN ESCLEROSIS MULTIPLE. / C-Phycocyanin Derived from Spirulina
Platensis Potentiates Alpha-IFN Induction of Regulatory T Cells in Multiple
Sclerosis.
Dra. Giselle Pentón Rol (PhD), CIGB, Cuba
PRODUCTOS NATURALES: RETOS PARA EL ACCESO A LA
DIVERSIDAD QUÍMICA Y NUEVOS BLANCOS TERAPÉUTICOS / Natural
Products: Challenges to Access the Chemical Diversity and New
Therapeutic Targets.
Dra. Evangelina Marrero Faz (DrSc), CENSA, Cuba.
Salas / Rooms
1,2,3
12:00-14:30
SALA 1/
ROOM 1
12:00-14:00
COMUNICACIONES ORALES / ORAL COMMUNICATIONS:
Simposio: Evaluación Farmacológica y potencialidades terapéuticas de
Productos Naturales. / Symposium: Pharmacological Evaluation and
Coordinadores /
Coordinators:
Dres. Milagros García Mesa, Cuba & André P. Boulet, Canada.
OC-15
12:00-12:20
OC-16
12:25-12:45
EL USO DE LOS PRODUCTOS NATURALES EN LA ENFERMEDAD
CRONICA HEPATICA Y HEPATOTOXICIDAD HERBARIA. / The Use of
Natural Products in Chronic Liver Disease and Herbal Hepatotoxicity.
Dr. Zeki Yesilova, Gülhane Military Medical Academy, Division of
Gastroenterology and Hepatology, Ankara, Turkey.
PRODUCTOS NATURALES COMO TRATAMIENTOS ALTERNATIVOS
DE LOS DESORDENES METABOLICOS OSEOS. / Natural Products as
Alternative Treatments for Metabolic Bone Disorders.
Dr. Cagatay Oktenli, GATA Haydarpasa Training Hospital, Division of
18
FAPRONATURA 2009
of Natural Products
OC-17
12:50-13:10
OC-18
13:15-13:35
OC-19
13:40-14:00
OC-20
14:05-14:25
SALA 2 /
ROOM 2
Internal Medicine, Istanbul, Turkey.
EL POTENCIAL ANTITROMBÓTICO DE LAS PLANTAS MEDICINALES. /
Antithrombotic Potential of Medicinal Plants.
Dra. Milagros García Mesa (PhD), INACV, Cuba.
DESARROLLO DE DROGAS BOTANICAS ANTI-INFLAMATORIAS
GRADO FARMACEUTICO: EXPERIENCIA CON PRODUCTOS DE
ORIGEN ORGANICO PUR 0110. / Developing Anti-Inflammatory
Pharmaceutical Grade Botanical Drugs: Experience with Organic Sourced
PUR 0110.
Dr André P. Boulet, PhD., President & COO, PurGenesis Technologies
Inc., Montmagny, Quebec, Canada.
ENSAYO CLINICO CONTROLADO Y RANDOMIZADO DE VIUSID EN
COMBINACION CON DIETA Y EJERCICIO EN PACIENTES CON
ESTEATOHEPATITIS NO ALCOHOLICA. / A Randomized Controlled Trial
of VIUSID in Combination to Diet and Exercise in Patients with Non Alcoholic
Steatohepatitis.
Dr. Eduardo Vilar Gómez
Nacional Institute of Gastroenterology, Havana City, Cuba.
INFLUENCIA DEL SUPLEMENTO NUTRICIONAL DIAMEL SOBRE
INDICES HOMA-B y HOMA-IR EN PACIENTES CON DIABETES
MELLITUS TIPO 2. / Influence of the Nutritional Supplement Diamel on
Indexes HOMA - B and HOMA-IR in Patients with Type 2 Diabetes Mellitus.
Dr. José A. Hernández-Yero, National Institute of Endocrinology, Havana
City, Cuba.
14:30-15:30
ALMUERZO / LUNCH
12:00-14:00
Simposio: Biodiversidad Marina Caribeña como Fuente de Nuevos
Compuestos de Interés Biomédico y otras Aplicaciones Industriales. /
Symposium: Caribbean Marine Biodiversity as a Source of New
Compounds of Biomedical Interest and others Industrial Applications.
Coordinadores /
Coordinators:
DrC. Roberto Núñez & MSc. Miguel David Fernández, CEBIMAR, Cuba.
OC-21
12:00-12:20
OC-22
12:25-12:45
OC-23
12:50-13:10
OC-24
13:15-13:35
UTILIZACIÓN DE BACTERIAS MARINAS PARA LA PRODUCCIÓN DE
PRODUCTOS NATURALES. / Utilization of Marine Bacteria for Production
of Natural Products.
Dr. Roberto Núñez, (PhD), CEBIMAR, Cuba
CARACTERIZACION
FARMACOLOGICA
DE
COMPUESTOS
BIOACTIVOS DE LA ANEMONA DE MAR EPICYSTIS CRUCIFER. /
Pharmacological Characterization of Bioactive Compounds from the Sea
Anemone Epicystis crucifer.
Dra. Anoland Garateix, (PhD) CEBIMAR, Cuba.
EFECTOS ANTIOXIDANTS Y CITOPROTECTORES DE BM-21, UN
EXTRACTO ACUOSO ETANOLICO DE THALASSIA TESTUDINUM EN
VARIOS MODELOS EXPERIMENTALES. / Antioxidant and Citoprotective
Effects of BM-21, an Aqueous Ethanol Extract from Thalassia testudinum in
Various Experimental Models.
Dr. Roberto Menéndez Soto del Valle, CEBIMAR, Cuba.
NUEVOS COMPUESTOS OBTENIDOS DE ESPONJAS DE LAS COSTAS
CUBANAS CON ACTIVIDAD FARMACOLOGICA. / New Compounds
Obtained from Sponges of Cuban Coasts with Pharmacological Activity.
Dr. Abilio Laguna, (PhD), CEBIMAR, Cuba.
19
FAPRONATURA 2009
of Natural Products
OC-25
13:40-14:00
OC-26
14:05-14:25
SALA 3 /
ROOM 3
UNA
INVESTIGACIÓN
SOBRE
USOS
POTENCIALES
PARA
AMPHITOXIN, UN PYRIDINIUM ALCALOIDE de LA ESPONJA
JAMAICANA, AMPHIMEDON COMPRESSA. / An Investigation of Potential
Uses for Amphitoxin, A Pyridinium Alkaloid from the Jamaican Sponge,
Amphimedon Compressa.
Dr. Winklet A. Gallimore. (PhD). The University of the West Indies, Mona
Campus, Kingston 7, Jamaica, West Indies.
UTILIDAD DE COMPUESTOS MARINOS ANTITUMORALES. / Useful
Antitumor Marine Compounds.
Dr. Sudeep Bhardwaj, Department of Pharmacology Seth G.L Bihani S.D
College of Technical Education Sriganganagar Rajasthan, India.
14:30-15:30
ALMUERZO / LUNCH
12:00-14:00
Simposio: Investigación y Desarrollo de Algunos Productos Naturales
cubanos. / Symposium: Research & Development of Some Cubans
Natural Products.
Dres. Octavio Fernández-Limia (CENSA) y Dulce María González
Mosquera (Universidad Central, Santa Clara), Cuba.
IMPACTO EN LA MORTALIDAD NEONATAL EN CUBA DEL USO DE UN
SURFACTANTE NATURAL “SURAFEN”. NUEVAS INDICACIONES
FUTURAS. / Impact on Cuba neonatal mortality of use of a natural
Surfactant “Surfacen”. Future new indications.
Dr. Octavio Fernández-Limia (PhD). National Centre for Animal and Plant
Health (CENSA), Cuba.
BIODISPONIBILIDAD DEL HIERRO EN LA LINEA DE PRODUCTOS
NATURALES TROFIN: USO DE DOBLE TECNICA RADIOISOTOPICA. /
Iron Bioavailability in the Product Line of Natural Origin Trofin: Use of the
Radioisotópica Double Technique.
Dr. Raúl González Hernández, PhD, BIOCEN, Cuba.
ACTIVIDAD ANTIVIRAL DE PHYLLANTUS ORBICUYLARIS HBK
CONTRA ENTEROVIRUS. / Antiviral Activity of Phyllanthus orbicularis HBK
Against Enteroviruses.
Lic. Maikel Acosta Zaldívar, Facultad de Biología, Universidad de La
Habana, Cuba.
NUEVO FLAVONOL CON ACTIVIDAD ANTI-INFLAMATORIA DE
BOLDOA PURPURASCENS, CAV. / New Flavonol with Anti-Inflammatory
Activity from Boldoa Purpurascens, CAV.
Dra. Dulce María González Mosquera (PhD), Department of Pharmacy
“Marta Abreu University”, Santa Clara, Cuba.
NUEVOS ENFOQUES ACERCA DE LA EFICACIA DE EXTRACTOS DE
RHIZOPHORA MANGLE L EN ULCERAS PEPTICAS EXPERIMENTALES.
/ New Approach of Rhizophora mangle L Efficacy In Experimental Peptic
Ulcers.
DrC. Luz María Sánchez Perera, CENSA, San José de Las Lajas, La
Habana, Cuba.
EXTRACTO CUBANO DE LA CORTEZA DE MANGIFERA INDICA L
(VIMANG): DE LOS ESTUDIOS PRECLINICOS A LA MEJOR
ESTRATEGIA DE INVESTIGACIÓN CLINICA: EL RETO PERMANENTE
DE LA FARMACOLOGIA DE PRODUCTOS NATURALES.. / Cuban
Extract From Stem Bark Of Mangifera Indica L (Vimang): From Preclinical
Studies To The Best Clinical Investigation Strategy: The Permanent
Challenge For The Pharmacology Of Natural Products.
Dr. René Delgado-Hernández, (PhD), CENCEC, Cuba.
Coordinadores /
Coordinators:
OC-27
12:00-12:20
OC-28
12:25-12:45
OC-29
12:50-13:10
OC-30
13:15-13:35
OC-31
13:40-14:00
OC-32
14:05-14:25
20
FAPRONATURA 2009
of Natural Products
SALON 1 /
ROOM 1
Salón Plenario/
Plenary Room
14:30-15:30
ALMUERZO / LUNCH
15:00-17:00
Actividades Recreativas y Libre Intercambio entre Delegados. / Recreational
Activities and Free Exchange Inter Delegates.
17:30-18:55
MINI SIMPOSIO. / Mini Simposium: ASPECTOS REGULATORIOS PARA
EL DESARROLLO DE PRODUCTOS NATURALES. EXPERIENCIA
CUBANA. / Regulatory aspects on natural products development. Cuban
experience.
Lecture 16
17:30-17:55
Lecture 17
18:00-18:25
Lecture 18
18:30-18:55
19:00-20:00
CENA / DINNER
Presentacion Especial “Flordis Natural Medicines”, Australia.
SALON 1 /
ROOM 1
Salón Plenario/
Plenary Room
Lecture 19
Coordinadores / Coordinators: MSc. Julián Rodriguez Alvarez
(CENCEC) and DrC Diadelis Remirez Figueredo, CECMED, Cuba.
MARCO REGULATORIO PARA EL DESARROLLO DE PRODUCTOS
NATURALES. / Regulatory framework for the development of Natural
Products.
MSc. Julián Rodríguez, CENCEC, Cuba.
ACTUALIZACIÓN SOBRE LOS REQUERIMIENTOS REGULATORIOS
PRECLÍNICOS Y CLÍNICOS EN CUBA. / Update in preclinical and clinical
regulatory requirements in Cuba.
Dra. Diadelis Remirez Figueredo (PhD), National Centre of the State
Quality Control of Drugs (CECMED), Cuba.
SISTEMA DE CONTROL DE LA CALIDAD PARA LOS ENSAYOS
CLÍNICOS CON PRODUCTOS NATURALES EN CUBA. / Quality
Management System for Clinical Trials with Natural Products in Cuba.
MSc. Sandra Álvarez Guerra, CENCEC, Cuba.
20:30-21:30
Dr. Andrea Zangara
Flordis Natural Medicines - International Business Development Manager,
Australia
21:30-23:30
ACTIVIDAD DE DESPEDIDA EN LA PLAYA / FAREWELL ACTIVITY IN THE
BEACH.
Música Cubana. Saludos Especiales a Delegados / Cuban Music. Special
Greetings to Delegates.
Salas /
Rooms 1,2,3
SALA 1 /
ROOM 1
09:00-11:10
09:00-11:10
OC-33
09:00-09:20
Simposio: Evaluación Farmacológica y potencialidades terapéuticas de
Productos Naturales / Symposium: Pharmacological Evaluation and
Dra. Milagros García Mesa, Cuba & Julio Cesar Escalona, Stgo de
Cuba.
INDUCCION DE CELULAS T REGULADORAS COMO UN MECANISMO
DE ACCION DE PRODUCTOS NATURALES EN ENFERMEDADES
AUTOINMUNES E INFLAMATORIAS CRONICAS. / Regulatory T Cells
Induction as a Mechanism of Action for Natural Products in Autoimmune
21
FAPRONATURA 2009
of Natural Products
OC-34
09:25-09:45
OC-35
09:50-10:10
OC-36
10:15-10:35
OC-37
10:40-11:10
SALA 2 /
ROOM 2
and Chronic Inflammatory Diseases.
Dra. Majel Cervantes, CIGB, Cuba.
ACTIVACION DE PKC POR ESTERES DE FORBOL AISLADOS DE
PLANTAS. / PKC activation by plant isolated phorbol esters.
Dra. Chryssa Dimaxi, Newcastle University, UK
PAPEL DE FLAVONOIDES BIOACTIVOS EN EL ESTRES OXIDATIVO
ASOCIADO A LA ESOFAGUITIS DE REFLUJO EXPERIMENTAL. / Role
of bioactive flavonoids against oxidative stress in experimental reflux
oesophagitis.
Prof. Ch.V. Rao, Pharmacology Laboratory, National Botanical Research
Institute, Uttar Pradesh, India.
INTERFERENCIA DE FLAVONOIDES SELECCIONADOS CON EL
CRECIMIENTO DE FILAMENTOS EN EL HONGO R NIGRICANS. /
Interference of selected flavonoids with the growth of filamentous fungus
R. nigricans.
Dr. Helena Lenasi, Institute of Biochemistry, Medical Faculty, University of
Ljubljana, Ljubljana, Slovenia.
ACTIVIDAD ANTIMICROBIANA DE EXTRACTOS DE HOJAS DE
TAMARINDUS INDICA L Y SU CORRELACION CON LA
COMPOSICION POLIFENOLICA Y FLAVONOLICA. / Antimicrobial
Activity of Tamarindus Indica L. Leaves Extracts and their Correlation with
Polyphenol and Flavonoid Composition.
Dr. Julio César Escalona Arranz, Universidad de Oriente, Santiago de
Cuba.
11:15-11:30
11:35-14:35
CONFERENCIAS Y CEREMONIA DE CLAUSURA / LECTURES AND
CLOSING CEREMONY
14:40-15:30
ALMUERZO DE CLAUSURA / LUNCH OF CLOSING
09:00-11:10
Simposio: ENSAYOS CLÍNICOS CON PRODUCTOS NATURALES /
Symposium: Clinical Trials with Natural Products.
Dra. Maria Acelia Marrero & Yisel Avila Albuerne, CENCEC, Cuba.
PERFIL ANALGÉSICO DEL EXTRACTO ACUOSO DE LA CORTEZA
DE MANGIFERA INDICA L (VIMANG®) EN PACIENTES CON DOLOR
CRÓNICO. / Analgesic profile of water extract of stem bark of Mangifera
indica L (Vimang®) in patients with chronic pain
Dra. Bárbara Beatriz Garrido Suárez (PhD), CENCEC, Cuba
COMPARACION DE LA TERAPIA REDUCTORA DE LIPIDOS CON
ATORVASTATINA Y POLICOSANOL EN EL ESTATUS REDOX EN
DISLIPEMIAS CON ENFERMEDADES VASCULARES PERISFERICAS.
/ Lipid-Lowering Therapy comparison with Atorvastatina and policosanol
on the redox status in dyslipidemics with peripheral vascular diseases.
Dr. Samuel Fleitas Estévez. Instituto Nacional de Angiología y Cirugía
Vascular (INACV), Ciudad de La Habana, Cuba.
EFICACIA DEL MIGRAPRECOL EN LA PREVENCION DE LA
MIGRANA. RESULTADOS DE UN ENSAYO CLINICO FASE III. /
Efficacy of Migraprecol in the Prevention of Migraine. Results of a Fase III
Clinical Trial.
Lic. Yisel Avila Albuerne, CENCEC, Cuba.
Coordinadores / Coordinators
OC-38
09:00-09:20
OC-39
09:25-09:45
OC-40
09:50-10:10
OC-41
10:15-10:35
VIUSID, UN SUPLEMENTO NUTRICIONAL EN PACIENTES CON
22
FAPRONATURA 2009
of Natural Products
OC-42
SALA 3 /
ROOM 3
10:40-11:10
HEPATITIS C CRONICA Y CIRROSIS. UN ESTUDIO CONTROLADO,
ALEATORIZADO Y A DOBLE CIEGO. / VIUSID, a Nutritional
Supplement, in Patients with Chronic Hepatitis C and Cirrhosis. A
Randomized and Double Blind- Controlled Study.
Dr. Eduardo Vilar Gómez, Nacional Institute of Gastroenterology, Havana
City, Cuba.
CONSIDERACIONES CLINICAS DE PRODUCTOS BOTANICOS. /
Clinical Studies Considerations of all Botanical Products.
Dra. Maria Acelia Marrero Miragaya, CENCEC, Cuba.
11:15-11:30
11:35-14:35
CLOSING CEREMONY
14:40-15:30
09:00-11:10
Simposio: MEDICINA TRADICIONAL Y ETNOFARMACOLOGIA /
Symposium: Traditional Medicine and Ethnopharmacology
OC-43
09:00-09:20
OC-44
09:25-09:45
OC-45
09:50-10:10
OC-46
10:15-10:40
OC-47
10:45-11:010
Dr. Andrew Scholey, Australia & DrC. Maria Boffil, Villa Clara Cuba.
BALAS MÁGICAS Y FLECHAS VENENOSAS: EL PAPEL DE LAS
MEDICINAS NATURALES EN EL TRATAMIENTO DE LOS
TRASTORNOS NEUROCOGNITIVOS Y LA NEURODEGENERA-CIÓN. /
Magic Bullets and Poison Arrows: The Role of Natural Medi-cines in
Treating Neurocognitive Decline and Neurodegeneration.
Prof. Andrew Scholey. Herbal and Nutritional Medicine Research Unit.
Brain Sciences Institute, Swinburne University, Australia.
ACEITE ESENCIAL LINALOE: REINCORPORACIÓN TERAPÉU-TICA. /
Linaloe Essential Oil: Its Therapeutic Reincorporation.
Lic. Eva Obregón Domínguez, Instituto Mexicano de Aromaterapia S.C.,
MEXICO
ACTIVIDAD AFRODISIACA DE CURCULIGO ORCHIODES GAERTN. /
Aphrodisiac Activity Of Curculigo Orchiodes Gaertn.
Dr. Nagendra Singh Chauhan, Department of Pharmaceutical Sciences,
Dr. H.S. Gour University, Sagar (M.P.), India.
EVALUACION PRECLINICA Y SEGURIDAD DE UN PRODUCTO
MEDICINAL
TRADICIONAL
PHELA,
UN
POTENCIAL
INMUNOMODULADOR MEDICINAL. / The Preclinical and Safety
Evaluation of a Traditional Medicinal Product, Phela, A Potential Immune
Modulator.
Dr. Motlalepula G. Matsabisa, Medical Research Council of South
Africa.
EL PAPEL DE LA EVIDENCIA CIENTIFICA EN LA ACTITUD DE LOS
CONSUMIDORES DE MEDICINAS HERBARIAS EN DIADEMA (SAO
PAULO, SUDESTE DE BRASIL. / The Role of Scientific Evidence on the
Attitudes of Herbal Medicine Consumers of Diadema (São Paulo,
Southeastern Brazil).
Dr. Juliana Lanini Mariano, Departamento de Psicobiologia, UNIFESP,
São Paulo, Brazil.
11:15-11:30
11:35-14:35
CLOSING CEREMONY
23
FAPRONATURA 2009
of Natural Products
14:40-15:30
Sala de
Conferencia
s / Room of
Lectures
11:35-14:35
CLOSING CEREMONY
CONFERENCIAS / LECTURES
Lecture 20
11:35-12:05
Lecture 21
12:10-12:40
Lecture 22
12:45-13:15
Lecture 23
13:20-13:50
13:55-14:30
Dr. Francisco Morón, Cuba / MSc. Marlene Porto, Cuba.
RESULTADOS EN LA INVESTIGACION FARMACOLOGICA PARA LA
VALIDACION DE PLANTAS UTILIZADAS EN LA MEDICINA
TRADICIONAL EN EL PROGRAMA TRAMIL, 1988-2008. / Results on
pharmacological research to validate traditional medicinal plant uses in
TRAMIL program: 1988-2008.
Dr. Francisco Morón. (PhD). Director, Laboratorio Central de
Farmacología, FCM Salvador Allende, Ciudad de La Habana, Cuba.
PRODUCTOS NATURALES, DESDE LA PRE A LA POST
COMERCIALIZACION. / Natural Products, Since Pre To Postmarketing.
Dra. Giset Jiménez, CDF, Cuba.
EL
DESARROLLO
DE
ENSAYOS
CLÍNICOS
EN
CUBA.
EXPERIENCIAS CON PRODUCTOS NATURALES. / Development of
Clinical Trials in Cuba. Experiences with Natural Products.
Dra. María Amparo Pascual López, Directora, CENCEC, Cuba.
EL DESARROLLO DE PRODUCTOS NATURALES EN CUBA.
EXPERIENCIAS DEL CENTRO DE INVESTIGACION Y DESARROLLO
DE MEDICAMENTOS. / Development of Natural Products in Cuba.
Experience of the Drug Research and Development Center.
MSc. Marlene Porto Verdecia, Directora General, CIDEM, Cuba.
CEREMONIA DE CLAUSURA / CLOSING CEREMONY
Dr. Emilio Monteagudo, Villa Clara, Cuba.
RELATORÍA
DE
LAS
PRINCIPALES
ACTIVIDADES
DESARROLLADAS DURANTE FAPRONATURA 2009 / Summary of
Principal Activities Developed During Fapronatura 2009.
Dres. Diadelis Remirez, Mario Landys Chovel, Idania Rodeiro and
René Delgado, Cuban Society of Pharmacology, Cuba.
14:30-15:30
16:00
PREMIOS Y RECONOCIMIENTOS DE LA SOCIEDAD CUBANA DE
FARMACOLOGÍA A LOS MEJORES TRABAJOS PRESENTADOS EN
FAPRONATURA 2009 / Awards and Recognitions of the Cuban Society of
Pharmacology to the Best Works Presented in Fapronatura 2009.
ALMUERZO / LUNCH
Salida / Departure
Regreso de delegados a sus países y provincias / Return of delegates to
their countries and provinces.
24
FAPRONATURA 2009
of Natural Products
TRABAJOS EN CARTELES / WORKS IN POSTERS
CARTELES / POSTERS
CARTEL
NO.
/
POSTER
NO.
PPP-01 PPP-39
PPP-01
CIUDAD Y
PAIS / CITY
AND
COUNTRY
AUTORES / AUTHORS
TITULO DEL TRABAJO / TITLE OF WORKS
CARTELES FARMACOLOGÍA PRECLÍNICA /
POSTERS PRECLINICAL PHARMACOLOGY
PISA, ITALIA
Ronca F., Tagliazucchi D., Maltinti
S., Gondres Amaro Y., Ronca G.
PPP-02
REGGIO
EMILIA, ITALIA
Tagliazucchi D., Verzelloni
Gondres Amaro Y., Conte A.
PPP-03
MADRID,
ESPAÑA
PPP-04
MORELOS,
MEXICO
Martín S., Gómez-Serranillos P.,
Ortega T., Palomino O., Carretero E.,
Prodanov M., Vacas V., Cabellos M.,
Estrella I., Hernández T.
Elizalde, J., León, I y GonzálezChristen, J
PPP-05
TINGO MARÍA,
PERÚ
Tello W, Guerrero T, Sandoval M
PPP-06
MÉXICO
MEXICO
D.F
PPP-07
MÉXICO
MEXICO
D.F
Prieto-Gómez B., Santibáñez-Olvera
C., Moreno-López Y. L., ReyesPrieto N. M. y Velázquez-Paniagua
M.
Velázquez-Paniagua
M.,
ParraGámez L., Gutiérrez-Ruiz J., GarcíaHidalgo A., Balderas-López Eduardo
J., and Prieto-Gómez B.
PPP-08
KAVALI, INDIA
DrC. CHANGAMMA
PPP-09
VILLA CLARA,
CUBA
PPP-10
SANTIAGO DE
CUBA, CUBA.
CIUDAD DE LA
HABANA,
CUBA
SANTIAGO DE
CUBA, CUBA.
Rayza Méndez Triana MSc, María
BoffIill Cárdenas PhD, Emilio E.
Monteagudo Jiménez MSc, Maykel
Pérez Machín MSc, Omaida López
Bernal MD.
Pajarín F L, Álvarez G RM.
PPP-11
PPP-12
PPP-13
PPP-14
PPP-15
PPP-16
PPP-17
E.,
Casado C., Gutiérrez Y., García G.,
López R., Miranda M.
CYTOTOXIC
ACTIVITIES
OF
PEROXIDASES
AND
HYPOHALOUS ACIDS. PROTECTION BY POLYPHENOLS AND
OTHER NATURAL COMPOUNDS.
BIOLOGICAL
ACTIVITY
OF
MELANOIDINS
FROM
TRADITIONAL BALSAMIC VINEGAR OF MODENA AND
REGGIO EMILIA.
INFLUENCE OF GRAPE VARIETY ON NEUROPROTECTIVE
AND VASODILATORY EFFECT OF RED WINES.
ESTUDIO DEL POTENCIAL CITOTÓXICO DEL GLICOLIPIDO
TETRASACARIDO STANCINA IV, DE Ipomea stans, SOBRE
LINEAS CELULARES.
CONTENIDO DE POLIFENOLES TOTALES Y CAPACIDAD
ANTIOXIDANTE EN EXTRACTOS ACUOSOS Y ETANÓLICOS
DE HOJAS Y CORTEZA DE Ochroma pyramidale (Cav. ex. Lam).
ANTIAGING ACTION OF MELATONIN
MELATONIN
REGULATES
BEHAVIOR OF MALE RATS.
FERTILITY
AND
SEXUAL
METABOLIC STUDIES ON THE ANTISPERMATOGENIC
EFFECT OF CARICA PAPAYA LINN. SEED EXTRACTION.
PROTECTIVE EFECT OF Bidens pilosa L EXTRACT IN
HEPATOTOXICITY INDUCED BY PARACETAMOL.
PRECLINICAL EVALUATION OF THE ANTI-INFLAMMATORY
ACTIVITY OF WHITE BIDENS (ROMERILLO).
ANTI-INFLAMMATORY, ANTIOXIDANT AND ANALGESIC
EVALUATION ON Murraya paniculata.
Gross Fernández MC.
ANTI-INFLAMMATORY ACTIVITY OF TWO FLAVONOLIC
EXTRACTS OF THE PETIVERIA ALLIACEA L. IN RATS.
CAMAGUEY,
CUBA
CAMAGUEY,
CUBA
CIUDAD DE LA
HABANA,
CUBA
VILLA CLARA,
CUBA
Ferrándiz D., Iglesias E., Valls AR.,
Pérez PL.
Pérez PL, Ferrándiz D, Iglesias E,
Valls AR.
Márquez L, Garrido G, Gómez B,
Garate I, Muñoz JL, Menchén L,
Lesa Cerro JC
Boffill M, Monteagudo E, Martínez
Y, Villanueva G, Quesada D.
ANTI-INFLAMMATORY AND CYTOTOXIC ACTION OF THE
THYME (THYMUS VULGARIS).
EVALUATION IN VIVO OF A FLAVONOID OF PIPER AURITUM
IN A MODEL OF RETARDED HYPERSENSITIVITY.
PROTECTIVE EFFECT OF MANGIFERA INDICA L. EXTRACT
ON DEXTRAN SULFATE SODIUM- INDUCED COLITIS IN RATS.
VILLA CLARA,
CUBA
Pérez M, Tamayo M, Boffill M,
Lorenzo G, Jiménez E, Betancourt E,
25
EFFECT OF POLICOSANOL FROM SUGAR CANE ON
INFLAMMATORY AND WOUND HEALING IN EXPERIMENTAL
MODELS.
EVALUATION OF THE DIURETIC ACTIVITY OF SIX PLANTS
USED BY THE CUBAN POPULATION.
FAPRONATURA 2009
of Natural Products
Iglesias N, Verdecía B, Díaz L.
PPP-18
PPP-19
PPP-20
PPP-21
PPP-22
PPP-23
PPP-24
PPP-25
PPP-26
BEJUCAL, LA
HABANA,
CUBA
CIUDAD DE LA
HABANA,
CUBA
SAN JOSÉ DE
LAS LAJAS, LA
HABANA,
CUBA.
SAN JOSÉ DE
LAS LAJAS, LA
HABANA,
CUBA.
CIUDAD DE LA
HABANA,
CUBA.
CIUDAD DE LA
HABANA,
CUBA.
CIUDAD DE LA
HABANA,
CUBA.
CIUDAD DE LA
HABANA,
CUBA.
SÃO PAULO,
BRASIL
PPP-27
VILLA CLARA,
CUBA
PPP-28
CIUDAD DE LA
HABANA,
CUBA
CIUDAD DE LA
HABANA,
CUBA
LA
PAZ,
BOLIVIA.
PPP-29
PPP-30
PPP-31
PPP-32
PPP-33
PPP-34
PPP-35
PPP-36
PPP-37
PPP-38
García Y, Martínez D, González R,
Pardo-Andreu GL
Torres A, León O, Casanova M,
Rodríguez G.
Janet Sánchez, Roberto Faure,
Gregorio
Martínez,
Octavio
Fernández, Ernesto Vega and
Evangelina Marrero.
Odalys B., Cruz A, Morales I, Faure
1
R and Pérez-Gil J
Magariño Y, López O, Coipel L and
Delgado R.
Rosario L, Rodeiro I, Almeida E,
Leyva O, Alonso A, Delgado R,
Rodriguez Y.
Vernhes M, González-Pumariega M,
Andrade L, Passaglia A, Moreno de
Lima-Bessa K, Menck CF and
Sánchez-Lamar A.
González-Púmariega, M, Vernhes,
M, Menck CF, Sánchez-Lamar, A.
Duarte-Almeida JM, Tempone AG,
Reimão JQ, Rodrigues E, Carlini EA.
Hidalgo PI, Anke T, Meffert A,
Medina RP, Peñate L, García MR,
Casanova M, Enríquez V
García M, Monzote L, Montalvo AM,
Scull R.
Monzote L, García M, Scull R,
Miranda M
Saavedra G, Poka D, Ruiz G.
CIUDAD DE LA
HABANA,
CUBA.
CIUDAD DE LA
HABANA,
CUBA.
CIUDAD DE LA
HABANA,
CUBA.
Acosta M, Álvarez AL, Sarmiento L,
García T, Acosta I, Spengler I,
Roque A, del Barrio G.
del Barrio G, Álvarez A.L, Acosta M,
Spengler I, García, T y F. Parra.
CIUDAD DE LA
HABANA,
CUBA.
Camaguey,
Cuba.
CIUDAD DE LA
HABANA,
CUBA.
SANTIAGO DE
CUBA, CUBA.
CIUDAD DE LA
HABANA,
Roque A, González R, Morier L,
Rodríguez LA, del Barrio G
Álvarez AL, Kourí V, Martínez PA,
Parra F, del Barrio G.
Chevalier P, León M, Hernández M,
Cedeño P, Vidal M
Echemendía O, Almora E, Fidalgo
O, Monteagudo R, Bolaños G, Lago
V.
Ochoa AP, Marín JM, Mujawimana
RJ, Tamayo KRG, González ZG.
Almora E, Monteagudo R, Fidalgo O,
Echemendía O, Bolaños G, García
26
IN VITRO EFFECT OF TROFIN®, AN HYDROLYZED OF
PROTEINS AND HEME IRON MIXTURE ON IONIC IRON
REDOX STATUS.
EFFECTS OF NATURAL ZEOLITE MODIFIED WITH ZINC (ZZ) ON
STREPTOZOTOCIN-INDUCED DIABETES MODEL IN RATS.
POLYPHENOL COMPOUNDS & PHARMACOLOGICAL REDOX
MECHANISMS
INVOLVED
IN
WOUND
HEALING.
RHIZOPHORA MANGLE CASE STUDY.
UPDATE OF EXPERIMENTAL BIOPHYSICAL AND RHEOLOGY
PROPERTIES OF NATURALS LUNG SURFACTANTS.
IN VITRO AND IN VIVO ANTI-TUMOUR EFFECT OF THE
AQUEOUS EXTRACT OF THE MANGIFERA INDICA L. STEM
BARK EXTRACT AND ITS MAIN COMPONENT MANGIFERIN.
EVALUATION IN VITRO OF THE RADIOPROTECTIVE EFFECT
OF THE AQUEOUS EXTRACT OF MANGIFERA INDICA L.
(VIMANG).
PROTECTIVE EFFECT OF THE Phyllanthus orbicularis, HBK
AQUEOUS EXTRACT ON HUMAN CELLS AGAINST THE
DAMAGE INDUCED BY UVB RADIATION.
THE PROTECTIVE CAPACITY OF Cymbopogon citratus (DC)
Staff EXTRACT AGAINST THE DAMAGE INDUCED BY
ULTRAVIOLET RADIATION
ANTIPROTOZOAL BIOMONITORED FRACTIONATION OF THE
BRAZILIAN PLANTS EXTRACTS HIMATANTHUS SUCUUBA
AND CAMPSIANDRA COMOSA.
SEARCH FOR NEW SECONDARY METABOLITES WITH
BIOLOGICAL ACTIVITY FROM ENDOPHYTIC FUNGI.
ANTILEISHMANIAL ACTIVITY OF BIXA ORELLANA.
CHEMISTRY
COMPOSITION,
CITOTOXICITY
AND
ANTILEISHMANIAL ACTIVITY OF THE ESSENTIAL OIL FROM
PIPER AURITUM.
EFFECT OF THE SEASONAL VARIABILITY IN THE
COMPOSITION AND THE BIOLOGICAL ACTIVITY OF THE
ESSENTIAL OIL OF SIPARUNA BIFIDA.
SEARCH FOR ANTIVIRAL ACTIVITY OF THE PLANT
AGERATINA HAVANENSIS AGAINST DNA AND RNA VIRUSES.
AGERATINA HAVANENSIS HBK EXTRACTS INHIBIT RABBIT
VESIVIRUS IN VITRO REPLICATION
PHYLLANTHUS
ORBICULARIS
INHIBITORY
ACTIVITY
AGAINST HERPES SIMPLEX VIRUSES AND HUMAN
CYTOMEGALOVIRUS: AN APPROACH TO ANTIVIRAL
MECHANISMS.
IN VITRO ANTIVIRAL ACTIVITY OF MEDICINAL PLANTS
AGAINST HEPATITIS B VIRUS (HBV).
THE COEFFICIENT OF SPECIFICITY OF INHIBITION AgsVBH
IN SPECIES CUBAN PHYLLANTHUS.
ANTIBACATERIAL ACTIVITY OF FOUR NATURAL PRODUCTS.
ANTIMICROBIAL EVALUATION OF TOTAL EXTRACTS FROM
LEAVES OF PETIVERIA ALLIACEA L. (ANAMÚ).
CITOTOXICITY OF FRACTIONS FROM OF THE ESPECIE
ERYTHROXYLUM MINOTIFOLIUM ON TWO CELLS LINES.
FAPRONATURA 2009
of Natural Products
PPP-39
PCP-01 PCP-08
PCP-01
PCP-02
PCP-03
PCP-04
PCP-05
PCP-06
PCP-07
PCP-08
PEP-01 PEP-03
PEP-01
CUBA.
M, Lago V, González JA.
CIUDAD DE LA
HABANA,
CUBA.
Díaz García CM, Sánchez Soto C,
Fuentes D, Rodríguez A, Pedroso A,
Hiriart Urdanivia M, Castañeda
Pasarón O.
INCREASED INSULIN SECRETION IN RAT BETA CELLS
TREATED WITH LOW MOLECULAR WEIGHT FRACTIONS
FROM Physalia physalis.
CARTELES FARMACOLOGIA CLINICA /
POSTERS CLINICAL PHARMACOLOGY.
EMPOLI,
ITALIA
Gori L, Mascherini V, Firenzuoli F
CIUDAD DE LA
HABANA,
CUBA
CIUDAD DE LA
HABANA,
CUBA
PINAR
DEL
RÍO, CUBA.
Guevara M, Pérez T, Calderón B,
Perdomo J, Morales C, Garrido G.
SANCTISPIRITUS,
CUBA.
CARDENAS,
MATANZAS,
CUBA.
VILLA CLARA.
CUBA
Ruiz Calabuch H, Jiménez J, Silva J,
Ramos L, Leon O, Betancourt J.
Granma, Cuba.
Barrese Y, Avila Y, Díaz E y
Fernández-Limia O
Valdés Carrillo R, Trasanco Delgado
M, Becerra N, Tur Behar R.
Marin N, López K, Sánchez to,
Vargas R.
Galbán M, Medina A, Pérez M,
Moreia A, Liste S, Artiles M, Santos
N, Suero B, Zaita Y, Martínez B,
Bauta R, Orozco M.
Ortiz Y, Martínez H, López T,
Cabrera Z.
FERMENTED RICE IN PATIENTS SUFFERING ADVERSE
REACTION
TO
SYNTHETIC
HMG-CoA
REDUCTASE
INHIBITORS: TREAMENT OF A SERIES OF 24 PATIENTS WITH
FERMENTED RED RICE.
ANTI-INFLAMMATORY EFFECT OF AN EXTRACT OF
MANGIFERA INDICA L. (VIMANG®) IN PATIENTS WITH
CHRONIC DERMATITIS.
EFFECTS OF SURFACEN IN LOW AND MAINTAINED DOSE IN
THE TREATMENT OF ARDS. PRELIMINARY RESULTS.
LEPIDIUM MEYENI (MACA). IT’S EFFECTS ON SEXUAL
DESIRE AND SERUM REPRODUCTIVE HORMONE LEVELS IN
A GROUP OF MALES OVER 50 YEARS OF AGE.
EVALUATION OF
EFFICACY AND SECURITY OF
DERMOFURAL OINTMENT IN PATIENTS WITH SQUAMOSA
AND CORPORIS TINEA PEDIS.
USE AND APPLICATION OF GARLIC (ALLIUM SATIVUM L) IN
VIRAL AFFECTIONS ON THE SKIN.
THE SAFETY AND EFFICENCY OF 20% HERBAL GUACAMAYA
FRANCESA IN THE TREATMENT OF INTERDIGITAL
EPIDERMOFOSIS INFECTION.
EFFECTIVENESS AND SEGURITY OF THE DYE OF
PEDILANTHUS TITHYMALOIDES (L) POIT (ITAMO REAL) IN
THE TREATMENT OF EDEMATOUS CHRONIC GINGIVITIS.
CARTELES ESTUDIOS ETHNOFARMACOLOGICOS Y DE UTILIZACION /
POSTERS ETHOPHARMACOLOGICALS AND USES STUDIES.
TRUJILLO,
VENEZUELA
Bermúdez A., Oliveira M., Carrillo T.
MEDICINAL PLANTS UTILIZED IN RURAL COMMUNITIES OF
THE ANDEAN PIEDMONT, VENEZUELA: Traditional uses and
relative importance.
PEP-02
TRUJILLO,
VENEZUELA
Carrillo T., Bermúdez A., Oliveira M.
PEP-03
CIUDAD DE LA
HABANA,
CUBA
Alfonso K., Collazo M., Fernández
M., Ballagas C.
UTILIZATION OF MEDICINAL PLANTS IN AN URBAN
COMMUNITY OF THE TRUJILLO STATE, VENEZUELA:
TRADITIONAL USES, RELATIVE IMPORTANCE AND PATTERN
OF DISTRIBUTION OF THE TRADITIONAL KNOWLEDGE.
A PHARMACOECONOMIC STUDY COMPARING A NATIONAL
NATURAL TOPIC PRODUCT WITH A FOREIGN TOPICAL
CREAM.
PTS-01 PTS-06
PTS-01
CARTELES ESTUDIOS TOXICOLOGICOS /
POSTERS TOXICOLOGICALS STUDIES.
VILLA CLARA,
CUBA
Monteagudo E, Boffill M, Betancourt
E, Quesada D, Blanco F, Díaz L,
Verdecía B, Iglesias N.
HYPOGLYCEMIC ACTIVITY AND ACUTE TOXICITY OF FOUR
PLATS POPULARLY USED IN CUBA.
PTS-02
VILLA CLARA,
CUBA
Saucedo Y, García D, Tenorio E,
Monteagudo E.
PTS-03
SANTIAGO DE
CUBA, CUBA
PTS-04
CIUDAD DE LA
HABANA,
CUBA.
CIUDAD DE LA
Berenguer CA, Alfonso A, Fong O,
Domínguez A, Betancourt JE,
Laramendi D, Salas H, Puentes E,
Wawoe N, Cedeño N.
Leyva O, Alonso A, Rodeiro I, Díaz
A, Rosario L, Carro S, GonzalezLavaut A
Sánchez-Lamar A, Cuevas A, Alonso
TOXICOLOGICAL EVALUATION OF POWDER OF DE
PARTHENIUM HYSTEROPHORUS, L; USING ALTERNATIVES
TWO TECHNIQUES.
TOXICITY STUDY TO A REPEATED DOSE OF THE
AZADIRACHTA INDICA A. JUSS PLANT (NIM´S TREE).
PTS-05
27
GENOTOXIC EVALUATION IN VITRO OF ERYTHROXYLUM
MINUTIFOLIUM, AN ENDEMIC CUBAN PLANT.
ASSESSMENT OF THE POTENTIAL GENOTOXIC EFFECT OF
FAPRONATURA 2009
of Natural Products
PTS-06
PAM-01 PAM-07
PAM-01
PAM-02
PAM-03
HABANA,
CUBA.
A, Almeida E, Verhnes M.
CIUDAD DE LA
HABANA,
CUBA.
Domínguez Pérez D, Díaz García
CM, Pérez Saad H, García Delgado
N, Varela C, Sierra Y, Fuentes D,
Acela Pedroso, Rodríguez A, Hiriart
M, Castañeda Pasarón O.
CARTELES ESTUDIOS DE MEDICINA ALTERNATIVA /
POSTERS ALTERNATIVE MEDICINES.
FAISALABAD,
PAKISTAN
SANTIAGO DE
CUBA,
CUBA
MATANZAS.
CUBA.
PAM-04
MATANZAS.
CUBA.
PAM-05
SANTIAGO DE
CUBA, CUBA.
LAS
TUNAS,
CUBA.
PAM-06
PAM-07
PPT-01 PPT-02
PPT-01
PPT-02
AQUEOUS
EXTRACTS
FROM
BRYOTHAMNION
TRIQUETRUM, HALIMEDA INCRASSATTA AND HALIMEDA
MONILE SEAWEEDS, USING SOS CHROMOTEST ASSAY.
TOXICOLOGICAL AND PHARMACOLOGICAL PROPERTIES OF
A LOW MOLECULAR WEIGHT FRACTION FROM ZOANTHUS
SOCIATUS (CNIDARIA) ASSESSED IN OF-1 MICE.
SANTIAGO DE
CUBA, CUBA.
Zafar MA, Mahfooz A, Abbas RZ,
Sohail M and Manzoor A
Yepes A, Aguilera E, Gómez R,
Martínez I, Aguilar B, Kindelán L.
Perdomo Delgado J, González Pla E,
Castro Morillo E, Páez Casona T,
Sánchez Hernández T.
Consuegra
Leon
L,
Perdomo
Delgado J, González Pla E, Trimiño
Galindo L, Otero Barroso M.
Domínguez González E.J.
Ferrada Zapatero D, Rodríguez
Gutiérrez M, Pupo Machado M, Vila
Rodríguez I, León Ramírez M, Ávila
Pérez Y, Labrada Vila I.
Herrero Aguirre H, Álvarez G. RM.
®
USE OF CHELLIDONIUM (A HOMEOPATHIC MEDICINE) FOR
THE TREATMENT OF ICTERUS IN CATS.
APPLICATION OF THE MAGNETIC INDUCTIVE STABILIZER
®
(EIMA ) AND OZONETHERAPY IN THE TREATMENT OF
PATIENTS WITH DISC HERNIA.
ACUPOINT INJECTION WITH MINIMAL DOSES OF STEROIDS
FOR JOINT PAIN RESISTANT.
HEMOSTATIC EFFECT OF ULTRA HIGH DILUTIONS OF
PHOSPHORUS ON SURGICAL PATIENTS.
SURGICAL ACUPUNTAURAL ANALGESIA OF CATARACT IN
GERIATRICS PATIENT.
EFFICACY OF RESCUE REMEDY IN THE TREATMENT OF
INTERMITENT MODERATE ASTHMA CRISIS.
EFFECTIVENESS OF THE USE OF THE SAMBUCUS IN THE
TREATMENT OF THE BRONCHIAL HIPERREACTIVITY IN
PEDIATRIC AGE.
CARTELES TECNOLOGIAS FARMACEUTICAS /
POSTERS PHARMACEUTICALS TECHNOLOGIES.
TRUJILLO,
VENEZUELA.
JEREZ A, CHINAPPI I, UZCATEGUI
M.
SOLAR DRYING CURVES FOR THREE MEDICINAL SPECIES.
CURVAS DE SECADO SOLAR PARA TRES ESPECIES
MEDICINALES.
OAXACA,
MEXICO
L. Lagunez Rivera, G. Vilarem
METHOD OF EXTRACTION CARVONE BY DIRECT INDUCTION
HEATING – ASSISTED MAGNETIC FIELD.
28
FAPRONATURA 2009
of Natural Products
RESÚMENES / ABSTRACTS
CONFERENCIAS DE APERTURA / OPENING LECTURES (OL)
OL-01
NATURAL AND TRADITIONAL MEDICINE IN CUBA. ANTECEDENTS, CURRENT SITUATION
AND FUTURE PERSPECTIVES.
Dr. MSc. Martha Pérez Viñas
Director of the National Center of Natural and Traditional Medicine. Email: [email protected]
Delegate of the Cuban Academy of Sciences in the South-South and North-South cooperation.
Origins of natural and traditional medicine in Cuba, native culture and first approvals are exposed. Current rules,
as well as the factors improving and impairing this medicine, and the work about safety and efficacy of natural
products are presented. Perspectives of future development, including the view towards we are directed, are
discussed.
OL-02
MODULATION OF INFLAMMATORY GENE EXPRESSION BY NATURAL COMPOUNDS
Guy Haegeman.
Director, Laboratory of LEGEST, University of Gent, Belgium. E-mail: [email protected]
Inflammatory diseases are wide spread among human mankind. Although these various affections appear
different according to the tissue affected, they have some general aspects in common, such as the production of
inflammatory cytokines, like Interleukin-6 (IL6) and others. Actually, IL6 is a pluri-potent cytokine, of which the
expression needs to be tightly regulated in order to keep the organism in a balanced and homeostatic condition.
The IL6 gene is induced by a dual activation scheme: i.e. 1) the cytoplasmic activation of the general stress
factor NF-kB, which after stimulation migrates to the nucleus and occupies its position on various gene
promoters, and 2) the activation of the nuclear kinase MSK1, that phosphorylates the NF-kB p65 subunit at Ser
276 (to generate a fully transcription-competent enhanceosome), as well as the Histon-3 tails at Ser 10 (that is
the onset of chromatin relaxation).
Glucocorticoid hormones (GCs) remain the mainstay for the treatment of various inflammatory disorders,
because of their great efficacy. The long-term usage of GCs is, however, overshadowed by the occurrence of
debilitating side-effects, like osteoporosis, skin and muscle atrophy, diabetes and neurological disorders.
GCs exert their functions through binding to the glucocorticoid receptor (GR), a transcription factor that regulates
genes in a positive or negative way. Direct binding of activated GR in the promoter of target genes is believed to
be the main pathway leading to metabolic gene expression (causing side-effects), whereas the interference of
GR with the activity of other transcription factors, such as NF-kB or AP-1, greatly contributes to its desired antiinflammatory capacities. Dissociated ligands aim to separate GR-mediated transcriptional activation from
transcriptional repression in order to achieve better side-effect profiles.
Plant extracts have been used for many centuries as a popular remedy against several health disorders. In
recent years, however, a renewed interest in folk medicine and herbal treatments is seen, and at present, the
healing capacities of plant-derived compounds are being analysed at the cellular and molecular level. We have
studied the molecular properties of a compound derived from the Withania species, growing in Palestine and
used by the migrating shepherds in the desert. The major compound of the extract (Withaferin A) is a particular
inhibitor of the activation pathway of NFkB, i.e. the main transcription factor for inflammatory gene expression
and cell survival. This compound was shown to strongly exhibit anti-inflammatory capacities, both in vitro (cell
29
FAPRONATURA 2009
of Natural Products
cultures) and in vivo (mouse). Current efforts for its usage include the specific killing of malignant cells, which
may be superior than chemotherapy or radiation therapy.
A stabilized compound (CpdA), originally derived from a shrub (salt bush) in the Kalahari deserts of South-Africa
and Namibia, has anti-inflammatory properties in vitro (i.e. in cell culture) as well as in vivo (mouse and rat).
Furthermore, CpdA displays ‘dissociated’ activities, thus repressing inflammatory gene transcription but not
stimulating steroid-driven gene expression. Compared to treatment with classical glucocorticoids, CpdA may
avoid the harmful side effects of cortisone and thus give a better ‘benefit-to-side effect’ ratio than the presently
used steroidal drugs. Moreover, CpdA does not lead to GC resistance, after prolonged treatment, like GCs do.
OL-03
PHARMACOGENETICS
OF
HERBAL
MEDICINE:
POTENTIAL
RELEVANCE
VULNERABILITY TO DEPRESSION AND ANTIDEPRESSANT DRUG EFFECT.
FOR
Adrián LLerena*; Pedro Dorado; Eva M. Peñas-LLedó.
Clinical Research Centre. Extremadura University Hospital and Medical School. Hospital Infanta Cristina,
Badajoz, Spain. www.cicab.es [email protected]
Herbal medicines are frequently used in combination with commercially available drugs, therefore interactions
may occur. These potential herb-drug interactions may occur at pharmacokinetics as well as at
pharmacodynamic phases. Drug-metabolising enzymes or transporters are involved in many herb-drug
interactions, mostly due to competitive interaction at cytochrome P450 system. Polymorphisms in cytohrome
P450 and transporters genes are related to the pharmacokinetic interactions. Secondly the potential interactions
at pharmacodynamic level should also be considered for some drugs. Among drug metabolizing enzymes
CYP2D6 is the most studied and clinical relevant for worldwide clinically used drugs (LLerena et al.; 2009). For
example the effects of commonly used herbal products (Ginkgo biloba, kava, garlic, evening primrose oi, and St.
John's Wort) on the catalytic activity of cytochrome P450 isoforms (CYP1A2, CYP2C9, CYP2C19, CYP2D6 and
CYP3A4). It was shown herbal products containing could potentially inhibit the metabolism of co-administered
medications whose primary route of elimination is via cytochrome P450, and thus may produce relevant clinical
drug interactions (Zou et al.; 2002). Moreover the patients perspective might be relevant, since they considered
regular prescription drugs to be more effective and more prone to adverse effects than herbal drugs. In this
respect it has been shown that health professionals often overlook patients' use of herbal drugs (Wallerstedt et
al.; 2005).
Herbal medicine it is associated with certain cultures. Thus, taking into consideration that some genotypes may
present variability across countries and ethic groups, the interethnic variability in herbal medicine should be a
major issue in Clinical Pharmacogenetics. Currently, the pharmacogenetics approach has been used mainly to
study drug-drug interactions, however taking into consideration the increase use of herbal medicine, not only in
undeveloped world but also in so called “industrialized” countries the study of potential herb-drug interactions
might be necessary in clinical pharmacogenetics (Dorado et al.; 2005).
Variability in drug metabolism is mainly determined by genetic factors, however the importance of environmental
(such as dietary, smoking, diseases etc.) and physiological factors has been also increasingly recognized
(LLerena et al.; 1995). Moreover, there are suggestions that drug-metabolizing cytochrome enzymes (CYPs)
may be involved not only in xenobiotic metabolism (drugs and herbs) but also in the biotransformation of
endogenous substances and therefore it has been demonstrated its relationship with personality in Spanish and
well as Cuban healthy volunteers (LLerena et al.; 2003, González et.; 2008). Moreover due to the CYPs
expression in the human brain, it can be hypothesized the potential relationship to normal psychological and/or
abnormal psychopathological processes including neurocognition (Peñas-LLedó et al.; 2009). Therefore, the use
of herbal products might be related not only to drug efficacy but also to the disease pathophisiology. This is due
to the role of pharmacogenetics factors (metabolism, transporters receptors etc) not only in relation to xenobiotic
but also in relation to endobiotic metabolism and effect. For example CYP2D6 and CYP2C9 has been related to
the metabolism of the antidepressant drug fluoxetine but also to depression (LLerena et al.; 2003 and 2004),
therefore the potential interaction of herbal drugs (i.e. St. John's Wort, also metabolised by CYP2D6) may be
clinical relevant at both disease mechanism and antidepressant drugs effects. In conclusion in clinical medicine,
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the potential interaction of genetic and environmental factors including the endogenous metabolism must be
taken into consideration when herbal medicine is used or self-administered.
These studies were supported by Research Grants (SAF2006/13589), CIBERSAM, PI06/1681 and CP/06/0030
(PD). The study was coordinated in Red Iberoamericana de Farmacogenética y Farmacogenómica RIBEF
(CYTED206RT0290), www.ribef.org. *Speaker Cuban Society of Pharmacology Meeting on Herbal Medicines
(Fapronatura2009) Varadero June 03-06 2009.
OL-04
A CLINICAL CENTER OF NATURAL MEDICINE IN A PUBLIC HOSPITAL: 12 YEARS OF
EXPERIENCE
Firenzuoli F, Gori L.
Clinical Center of Natural Medicine (Dir.: F. Firenzuoli, MD), S.Giuseppe Hospital, Az USL 11 Empoli, ITALY
www.naturamedica.net E-mail: [email protected]
The clinical center of Natural Medicine of S. Giuseppe Hospital (Az. USL 11 of Empoli) is the referring regional
center for herbal medicine and is a public multidisciplinary center that offer different types of medical services:
phytotherapy, ear acupuncture, chirotherapy and Chinese acupuncture, both for the adult and pediatric patient.
Patients can access to the services through the regional prescription request as every other kind clinical or
laboratory medical service. The center offer biochemical analysis of natural products and on line counsealing for
adverse reactions, with a special service dedicated to pregnancy and breastfeeding. The center is affiliated to
the Italian National Institute of Health for evaluation of adverse reactions that can be signaled also through our
web site. The center organizes at least 2 national congress every year, and an international congress every 3
years. The clinical center of natural medicine is particularly involved in education at all levels, and has a special
master of clinical phytotherapy in a full joint university program with the university school of medicine of
Florence. We devoid particular attention to international bodys and we have active scientific collaborations.
CONFERENCIAS / LECTURES (L)
L- 05
COGNITION-ENHANCING EFFECTS OF GINKGO AND GINSENG.
Scholey, A & Stough, C.
National Institute of Complementary Medicine (NICM) Collaborative research Centre in Neurocognition and
Natural medicines, Brain Sciences Institute, Swinburne University
PO Box 218 Hawthorn, Victoria, 3122 AUSTRALIA E-mail: [email protected]
There is a wealth of research indicating that ginkgo biloba and ginseng may have cognition-enhancing effects.
This paper will briefly review evidence from double-blind, placebo-controlled human trials of the efficacy of a
Ginkgo biloba and Panax ginseng alone and in combination as cognitive enhancers in various populations.
Studies of the acute neurocognitive effects of standardized extracts of Ginkgo and Ginseng in healthy young
adults revealed that the combination was a more effective memory enhancer than either extract in isolation.
Furthermore a ginkgo-ginseng combination increased performance during heavily-loaded cognitive processing in
a synergistic manner that could not be predicted from the effects of either extract alone. Other studies have
revealed positive neurocognitive effects of a Ginkgo-Ginseng combination following chronic dosing in certain
populations. These findings also illustrate some of the issues and challenges involved in conducting clinical trials
into the neurocognitive effects of herbal extracts.
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L-06
HUMAN CLINICAL TRIALS WITH THE INDIAN SUPER MEDICINE BACOPA MONNIERA
(BRAHMI)
Stough, C., & Scholey, A.
PO Box 218 Hawthorn, Victoria, 3122 AUSTRALIA. e-mail: [email protected]
Bacopa monniera or brahmi has been used for centuries in India to improve cognitive functioning. More recently
there has been an explosion of in vitro and in vivo animal research focusing on its neurochemistry and mode of
action. In this presentation we discuss research on the Indian natural medicine brahmi in both animals and in
human trials assessing neurocognitive functioning. We start off with a brief discussion of the mechanisms of
action of brahmi generating hypotheses about its action on the human brain and on human cognition/
intelligence. A review of available human clinical trials including their samples, outcome measures and
conclusions is then presented. We conclude the presentation with a discussion of future applications and trials
with this interesting natural medicine.
L- 07
MEMORY ENHANCING EFFECT OF BACOPA MONNIERA.
Hemant K. Singh, Ph.D.
(ex-Deputy Director, Central Drug Research Institute, Lucknow).
Lumen Marketing Company 2, First Cross Street, Second Avenue, Ashok Nagar, CHENNAI - 600 083. E-mail:
[email protected]
Bacopa monniera has been used as a nerve tonic since Vedic civilization (c. 1500 B.C.). It has been frequently
mentioned in WHO recognized Ayurvedic treatises as treatment of choice for various brain and specially
memory disorders.
Because of its unique pharmacological properties, the plant attracted attention of several researchers in India. A
systematic chemical, pharmacological and pre-clinical investigations on this plant was undertaken by the Central
Drug Research Institute, Lucknow. Chemical investigations showed that bacosides A and B are the main
constituents of the plant, of which bacoside A is the predominant. Bacoside A was further shown to be having
different moieties of bacogenins. A standardized extract of the plant containing an optimum concentration of
bacosides vis-a-vis memory enhancing effect was developed. The extract was found to be significantly
improving acquisition, consolidation and retention of newly acquired learning behaviors in variety of responses. It
also attenuated pharmacologically induced amnesia in several animal models. The extract was found to be
equipotent to Gingkgo biloba in enhancing memory and had significantly pronounced anti-stress and
adaptogenic effects as compared to ginseng. This standardized extract also showed significant anti-oxidative
and neuroprotective effects.
The extract was found to be safe in acute and sub-acute (90 days) regulatory toxicity studies. Clinically it
showed significant improvement in memory parameters in children (6-13 years), adults (25-55 years) and elderly
(56-75 years) human volunteers in several learning parameters. It was found to be safe, well-tolerated and
devoid of any significant side effect.
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L-08
PHYTOTHERAPEUTICALS FOR BEHAVIOURAL AND PSYCHOLOGICAL SYMPTOMS OF
DEMENTIA
Okello, E & Perry E
Executive Director of the Medicinal Plant Research Group, School of Agriculture, Food and Rural Development.
Agriculture Building, Newcastle University, Newcastle upon tyne NE13GPF, UNITED KINGDOM E-mail:
[email protected]
Alzheimer’s disease (AD), the most common form of dementia is characterised by cognitive dysfunction and in
most cases associated behavioural and psychological symptoms such as depression, agitation, stress and
anxiety, which may lead to institutionalisation. Two of the current prescription drugs for dementia are
acetylcholinesterase (AChE) inhibitors derived from plants. Galantamine was originally from Galanthus and
Narcissus species and rivastigmine is based on the chemical structure of physostigmine from Physostigma
venenosum. There is clinical evidence relating to cognitive enhancement for other plants including extracts from
the European sage (Salvia officinalis), lemon balm (Melissa officinalis), the Indian herb Brahmi (Bacopa
monniera), and for some complex mixtures of traditional Chinese / Japanese medicines, Ginkgo biloba, and the
AChE inhibitor huperzine A from the moss Huperzia serrata. Both M. officinalis and Lavandula angustifolia
alleviate agitation and there is unexplored potential for other plants to alleviate symptoms, such as Hypericum
perforatum for depression and Valeriana officinalis for anxiety and sleep disorders. The potential application of
these species for dementia therapy is examined from the joint perspectives of traditional medical uses, relevant
mechanistic bioactivities and clinical controlled trial evidence. In addition epidemiological and related evidence
for the value of plant based agents such as nicotine and curcumin, red wine or fruit and vegetable juices for
prevention is reviewed.
L-09
PANAX GINSENG (G115) IMPROVES ASPECTS OF WORKING MEMORY PERFORMANCE,
MOOD IN HEALTHY YOUNG ADULTS.
Reay JL1, Kennedy DO1, Scholey AB2
1
Brain Performance and Nutrition Research Centre, Northumbria University, Newcastle upon Tyne, UK, NE1
8ST.
2
Brain Science Institute, Swinburne University, Melbourne, VIC 3122, Australia.
The term ‘Ginseng’ usually refers to the dried root of several species in the plant genus Panax (Araliaceae
family). With regards to human trials, several acute studies have shown that 200mg of the standardised extract
G115 is capable of reducing the subjective increase in mental fatigue associated with effortful cognitive
processing and improving mental arithmetic performance (Reay et al., 2005; 2006). Additionally, a series of
studies have reported improved memory performance following a single dose of G115 in healthy young
volunteers (see Kennedy et al., 2003 for review). Despite growing empirical support for the beneficial effects
following a single dose of G115, there is a lack of empirical research investigating the effects following repeated
dosing. The present placebo controlled, double-blind, balanced- crossover study assessed the effects of 200
mg and 400 mg Panax ginseng (G115) and placebo on mood, secondary memory and aspects of working
memory, following acute and sub-chronic (7 days) ingestion in healthy volunteers. Thirty healthy young adults
(mean age 22.87 years; SD 4.01) received each of the treatments for 8 days, in counterbalanced order and with
a 6 day wash-out between treatments. They completed a battery of tasks on the first (treatment with each of the
three treatment condition Day 1) and last (Day 8) day of taking each treatment, at pre-dose and thereafter at
1hour, 2.5hours and 4hours post-dose. Results revealed acute effect; however, 7 consecutive days of G115 led
to no additional additive effect. Planned comparisons revealed that 200mg was capable of improving subjective
ratings of calmness on Day 1 and Day 8; whereas, the effect was restricted to Day 1 following 400mg. With
regards cognitive performance, planned comparisons revealed an unexpected improvement following 400mg
G115 (given that previous studies have shown 200mg to be the optimal dose) on some aspects of working
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memory. In conclusion, 200mg G115 modulates subjective mood, which may be associated with alleviating
fatigue or stress that volunteers feel throughout the day; whereas, 400 mg appears to modulate cognitive
function. More research is needed.
L-10
- Presentación del Libro: Los retos de la Terapia Antioxidante. Colectivo de Autores. (Alberto Núñez-Sellés,
René Delgado, Mariela Guevara, Idania Rodeiro, Dagmar García, Gilberto Pardo, Beatriz Garrido y otros.)
L-11
- Presentación del Libro: PLANTAS MEDICINALES. Guía para su uso en la Atención Primaria de Salud.
MsC. Dr. Nelson Melgarejo López, MsC.Dra. Guadalupe Álvarez.
PLANTAS MEDICINALES
Guía para su uso en la atención primaria de la salud
Autor: Melgarejo López / Álvarez Bustamante / Abad
Editorial: Corpus
Formato: 16 x 24 cm
Páginas: 112
Sustrato interior: Papel ilustración
1º Edición 2008
Idioma: Español
Encuadernación: Rústica
ISBN: 9789509030640
EAN: 9789509030640
País de impresión: Argentina
Peso: 0,232 kg
Comentario
Esta obra sintetiza la experiencia cubana en el uso de plantas medicinales para la atención primaria de
salud. Sus autores nos brindan generosamente tanto el modo de empleo, la vía de administración y terapéutica
así como también las fórmulas magistrales de preparación farmacéutica. Encontramos también: nombres común
y científico, principios activos que les confieren propiedades medicinales, usos principales por aparatos y
sistemas y tablas que reflejan la forma natural de uso, los productos que se pueden adquirir en la farmacia, cómo
administrarlos, cuándo están contraindicados y qué precauciones se requieren. Además, se incluyen dos
apéndices: en uno se resumen los usos principales por aparatos y sistemas, y en el otro, las propiedades
terapéuticas de las plantas. Finalmente, contiene un breve Glosario para facilitarle al lector no especializado la
comprensión de algunos términos.
La obra ha sido elaborada por especialistas en medicina natural y tradicional, con un lenguaje sencillo,
al alcance no sólo de los médicos de Atención Primaria, farmacéuticos o botánicos, sino de todas
aquellas personas interesadas en los beneficios de las plantas medicinales descritas.
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Contenido
Plantas medicinales: Características y modo de empleo
Ají picante Capsicum annuum L.
Ajo Allium sativum L.
Albahaca blanca Ocimum basilicum L.
Anamú Petiveria alliacea L.
Añil cimarrón Indigofera suffruticosa Mill.
Bija Bixa orellana L.
Caisimón de anís Piper auritum Kunth
Calabaza Cucurbita moschata (Duch. ex Lam.) Duch. ex Poir.
Caléndula Calendula officinalis L.
Caña santa Cymbopogon citratus
(DC.) Stapf
Cañandonga Cassia grandis L. f.
Cebolla Allium cepa L.
Escoba amarga Parthenium hysterophorus L.
Eucalipto Eucalyptus citriodora Hook. Eucalyptus globulus Labill.
Guacamaya francesa Senna alata (L.) Roxb.
Guayaba Psidium guajava L.
Hierba buena Mentha spicata L.
Hinojo Foeniculum vulgare Mill.
Ítamo real Pedilanthus tithymaloides (L.) Poit.
Jengibre Zingiber officinale Rosc.
Limón Citrus aurantifolia (Christm.) Swingle
Llantén mayor Plantago major L.
Llantén menor Plantago lanceolata L.
Maíz Zea mays L.
Majagua Hibiscus elatus Sw.
Mangle rojo Rhizophora mangle L.
Manzanilla Matricaria recutita L.
Mastuerzo Lepidium virginicum L.
Menta japonesa Mentha arvensis L.
Mostaza Brassica juncea (L.) Czern.
Naranja Agria Citrus aurantium L.
Naranja dulce Citrus sinensis (L.) Osbeck
Orégano francés Plectranthus amboinicus (Lour.) Spreng.
Pasiflora Passiflora incarnata L.
Pino macho Pinus caribaea Morelet
Plátano Musa X paradisiaca L.
Quita dolor Lippia alba (Mill.) N.E.Br.
Romerillo Bidens pilosa L.
Romero Rosmarinus officinalis L.
Sábila Aloe vera (L.) Burm. f.
Salvia de Castilla Salvia officinalis L.
Té de riñón Orthosiphon aristatus (Blume) Miq.
Tilo Justicia pectoralis Jacq.
Toronjil Melissa officinalis L.
Toronjil de menta Mentha X piperita L.
Vicaria blanca Catharanthus roseus (L.) G.Don
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Aclaraciones de importancia para el facultativo, el farmacéutico y laboratorios de producción
Apéndice 1. Usos principales de las 46 plantas por aparatos y sistemas
Apéndice 2. Usos terapéuticos de las 46 plantas medicinales
Glosario
Referencias bibliográficas
Índice analítico
PLANTAS MEDICINALES GUÍA PARA SU USO EN LA ATENCIÓN PRIMARIA DE SALUD.
Melgarejo N1, Alvarez G2, Alonso A3
1
Centro Integral para el Desarrollo de la Medicina Natural y Tradicional (CIDMEDNAT-HV) “Jesús Montané
Oropesa de La Habana Vieja, 2Vicerrectoría Docente del Instituto Superior de Ciencias Médicas de La Habana
(ISCM-H) ,3Ministrio de la Agricultura.
Este libro representa la obra de dos médicos y un ingeniero agrónomo, cubanos, interesados en poner al
servicio de los profesionales de la salud, y de los lectores en general, una guía práctica para el uso de plantas
medicinales.
La temática abordada se presenta con un lenguaje claro y sencillo, al alcance de todas aquellas personas
interesadas en los beneficios que reporta su empleo sobre la salud humana.
Gran parte de las plantas incluidas en esta obra, son conocidas y empleadas en el mundo con muy buenos
resultados en el alivio de ciertas dolencias y malestares comunes; usted puede encontrarlas en cualquier latitud
del planeta, solo con la distinción de que en muchas localidades y países, el nombre común cambia por la
influencia de culturas y costumbres, no obstante mantienen sin variación su nombre científico; tampoco sufren
modificación significativa los principios activos y componentes de las mismas, por lo cual se conserva su
carácter terapéutico.
De manera resumida y didáctica, usted encontrará en esta guía;los principales usos por aparatos y sistemas,
las formas naturales o farmacéuticas de presentación, su modo de empleo, la dosificación, las precauciones
que debe tener en cuenta para su elaboración y administración, algunas contraindicaciones; entre otros
aspectos de interés.
Además, podrá consultar un índice de formulas farmacéuticas, un breve glosario para facilitarle al lector no
especializado la comprensión de algunos términos y una galería de imágenes que estamos seguros le serán de
gran utilidad.
Dispondrá no solo de la forma natural de preparación de las plantas, sino también de la formulación para que el
producto solicitado por usted pueda ser elaborado en la farmacia o laboratorio más cercano.
Hasta la actualidad se han realizado diversos estudios científicos en el campo de las plantas medicinales, sin
embargo, no se dispone de una publicación que oriente adecuadamente su manejo, objetivo que nos trazamos
con nuestra guía encaminada a dotar de una herramienta práctica y eficiente a todas aquellas personas
interesadas en el estudio de la fitoterapia.
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L-12
MODULATOR EFFECTS OF VIMANG, MANGIFERIN AND GALLIC ACID ON PROLIFERATION,
GENES EXPRESSION AND TRANSDUCTION SIGNAL PATHWAYS IN MDA-MB 231 CANCER
CELL LINE.
García-Rivera D1, Vanden Berghe W2, Delgado R3, Haegeman G2.
1
Department of Center for Biomolecular Chemistry. Havana. Cuba. [email protected]
2
Laboratory of Eukaryotic Gene Expression and Signal Transduction (LEGEST), Department of Molecular
Biology, Gent University. Belgium.
3
National Center for Clinical Trials. Havana. Cuba.
Vimang is a natural Cuban extract popularly used as complement of cancer treatment; despite the molecular
mechanisms of its possible antitumoural effects were still poorly studied. The aim of this study was evaluated
the molecular mechanisms of action of Vimang, mangiferin and gallic acid (two of the main components) in the
proliferation, gene expression and NFκB and MAPK-kinases pathway in MDA-MB231 breast cancer cell line.
The methodologies used were Northern blot for proteins expression, ELISA for proteins quantification and RTQPCR for gene expression. The first evidence was the antiproliferative response showed after 48 hours of
treatment with Vimang (IC50 259 µg/mL) and gallic acid (IC50 10 µg/mL), but mangiferin didn´t inhibit the
proliferation of these cells. After TNF cellular stimulation, it was demonstrated the modulation of NFκB activation
by Vimang (200 g/mL), mangiferin (100 µg/mL) and gallic acid (10 µg/mL). Three compounds inhibited the
IKK  phosphorylation, IkB degradation and interesting also reduced the IκB resynthesis. Then, p65
translocation into the nucleus and DNA-binding were reduced by the pretreatment with them. The treatment
with Vimang, mangiferin and gallic acid reduced the expression of NFκB driven genes: IL-6, IL-8, COX-2 and
VEGFA, and also reduced the IL-6 and IL-8 proteins levels. Vimang (200 µg/ml) and gallic acid inhibited the
activation of the MAPKinases pathway, reducing phosphorylation in MEK1/2 and MSK-1, ERK1/2, p90RSK and
S6. Mangiferin didn´t interfere with MAPK pathway. In summary, in this study has been identified a number of
target molecules modified for the treatment with Vimang in metastatic breast cancer cells, which could be
important in its application in the treatment of cancer. Also, it was demonstrated the contribution of gallic acid
and mangiferin on these activities.
L-13
CATECHOL CONTAINING POLYPHENOLS, MITOTOXICS OR MITOPROTECTORS? THE
MANGIFERIN TALE.
1
Pardo Andreu GL, 2Delgado Hernández R
Universidad de La Habana, Instituto de Farmacia y Alimento (IFAL), Centro de Investigaciones y Evaluaciones
Biológicas (CIEB). Calle 222 & 23 Av., No 21425 Código Postal 13600 Ciudad de la Habana, Cuba.
[email protected]
2
Centro Nacional Coordinador de Ensayos Clínicos (CENCEC) Calle 200 e/ 19 y 21, CP 11600, Playa, Ciudad
de La Habana, Cuba. Telef: (537) 271-8331 / 271 8342 Telfax: (537)-272 0653.
1
Mangiferin acts as a strong antioxidant on mitochondria (Eur.J.Pharmacol. 513:47-55, 2005). However, when in
the presence of Ca2+, mangiferin elicits mitochondrial permeability transition – MPT (Arch.Biochem.Biophys.
439:184-193, 2005), as evidenced by cyclosporin A-sensitive mitochondrial swelling. We propose that Ca2+
increases levels of mitochondria-generated ROS, which reacts with mangiferin producing quinoid derivatives,
which in turn react with the most accessible mitochondrial thiol groups, thus triggering MPT. It seems probable
that the free radical scavenging activity of mangiferin shifts its antioxidant protection to the thiol arylation. An
interesting proposition is that accumulation of mangiferin quinoid products would take place in cells exposed to
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an overproduction of ROS, such as cancer cells, where the occurrence of MPT-mediated apoptosis may be a
cellular defense mechanism against excessive ROS formation. We also provided evidence, by means of
electrochemical and UV-Visible spectroscopical analysis, that Fe(III) coordinates with mangiferin. The resulting
mangiferin-Fe(III) complex does not elicit MPT, and prevents MPT by scavenging reactive oxygen species.
Indeed, the complex protects mitochondrial membrane protein thiols and glutathione from oxidation. Fe(III) also
significantly increases the ability of mangiferin to scavenge the 2,2-diphenyl-1-picrylhydrazyl radical, as well as
to display antioxidant activity towards antimycin A-induced H2O2 production and t-butyl hydroperoxide-promoted
membrane lipid peroxidation in mitochondria. We postulate that coordination with Fe(III) constitutes a potential
protective mechanism towards the prooxidant action of mangiferin and other catechol-containing antioxidants
regarding MPT induction. We therefore envisage for mangiferin and perhaps for other cathechol-containing
polyphenols, a potential use either in preventing or eliciting cell death, simply in connection with the availability
of free/labile-bound iron in the cell environment. These circumstances should be taken into account during
human polyphenol supplementation, for either the prevention or not of their prooxidant effects, as well as for
optimization of their antioxidant performance.
L-14
C-PHYCOCYANIN DERIVED FROM SPIRULINA PLATENSIS POTENTIATES
INDUCTION OF REGULATORY T CELLS IN MULTIPLE SCLEROSIS.
ALPHA-IFN
Pentón-Rol G1*, Martínez-Sánchez G1Λ, Cervantes-Llanos M1*, Valdivia-Acosta AΛ, Acosta-Medina EΛ,
Lagumersindez-Denis NΛ, Falcón-Cama V*, Alonso-Ramírez Rδ, Cabrera-Gómez JA&, Lara Rodríguez Rµ,
Valenzuela-Silva C*, Rodríguez-Jiménez E*, López-Saura PA*, Guillén-Nieto GE*, Pentón-Arias E*.
*Center for Genetic Engineering and Biotechnology (CIGB). ΛCenter for Research and Biological Evaluations,
Institute of Pharmacy and Food Sciences, University of Havana (IFAL). δCenter of Molecular Immunology (CIM).
&
International Center of Neurological Restoration (CIREN). µ“Calixto García” Hospital, Havana City, Cuba.
Background: c-Phycocyanin (c-Pc), a natural antioxidant derived from the blue-green algae Spirulina platensis,
has been reported to exhibit pharmacological properties such as the capacity to inhibit cyclo-oxigenase 2, the
inflammatory processes and production of prostaglandin, leukotrienes and TNF-alpha, all of which may be
expected to symptomatically improve Experimental Autoimmune Encephalomyelitis (EAE) and Multiple
Sclerosis (MS). However, in this study we reveal a basic underlying mechanism that may provide an in-depth
approach to the rationale of the overall beneficial effect of this natural biliprotein. Materials & Methods: PBMC
from MS patients' were divided into four experimental groups: IFN-alpha treated cells, c-Pc treated cells, IFNalpha/c-Pc treated cells and untreated cells. Genes of regulatory T cells (Treg) markers were amplified and the
CD4+CD25highFoxp3+ subset of different experimental groups was measured by flow cytometry. EAE model
was developed in Lewis rat in order to assayed c-Pc effect. Clinical scores, oxidative stress markers and
microscopic studies were evaluated. Results: We found that in PBMC from MS patients and healthy controls
IFN-alpha/c-Pc provokes a CD4+CD25high+Foxp3+ rTc response. Moreover, we have found that c-Pc is able to
trigger mechanisms preventing EAE expression or downgrades its symptom scale score. Transmission electron
microscopy studies of brain samples also corroborated the improvement induced by c-Pc of the EAE myelin and
axonal damage. Conclusion: These results show that rTc may be the main and the essential contributor to
support the therapeutic potential of IFN-alpha/ c-Pc combination for MS treatment. Other autoimmune and
neurological diseases could also benefit from these findings.
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L-15
NATURAL PRODUCTS: CHALLENGES TO ACCESS THE CHEMICAL DIVERSITY AND NEW
THERAPEUTIC TARGETS.
Evangelina Marrero Faz, DrSc
Grupo de Química, Farmacología y Toxicología Centro Nacional de Sanidad Agropecuaria, CENSA, Apdo. #10,
San José de las Lajas, La Habana, Cuba. [email protected]
Natural products (NPs) represent the most important source of new commercially viable drug leads because the
chemical diversity and novelty associated with natural sources is higher than that of any other source. It is
particularly important when searching for lead molecules against newly discovered targets for which there are no
known small molecule leads. Regardless those mentioned advantages of NPs the industrial drug discoveries not
always are enthusiastic to approach research projects. Despite the believed assumptions, NPs can be a more
economical source of chemical diversity compared with synthesis of equivalent numbers of diverse chemicals. In
addition, less than 10% of the world’s biodiversity have been tested for biological activity, so still there are many
useful lead compounds waiting to be discovered from natural sources. Moreover, NPs that are found to be
biologically active in pharmacological assays are generally small molecules with drug-like properties, it means
capable of being absorbed and metabolised by the body. The challenge is how to access to the great chemical
diversity. Several different strategies are emerging in the post genomic era where a good deal of research have
been approached for a better understanding of the molecular mechanism involved in the signalling pathways of
different diseases oriented to find new promising therapeutics agents, being NPs of major attention.
Pharmacological studies to obtain new molecules that concerns different relevant diseases as cancer, type 2
diabetes, antimicrobials, among other, obtained from natural sources constitute a hopeful research field where
the great biodiversity observed mainly in tropical countries is still waiting to be explored. The present work brings
information and examples on different and valuable strategies that are emerging to explore the pharmacological
activity and chemical structure on NPs in relatively short time comparing with that of classic methods: eg. the
molecular mechanisms involved receptors PTP-1B, PPAR α/γ among other therapeutic targets associated to
type 2 diabetes and related metabolic disorders. The specificity of the mechanism associated to particular
pharmacological targets brings also the opportunity to investigate the pathogenesis of definite disease at cellular
and molecular level and also offers the opportunity to establish the mechanism of action involved in the
traditional use of medical plant. The in vitro evaluation of natural extracts oriented to obtain new active
molecules constitutes a valuable tool for the screening of the biological activity in an appropiate therapeutic
target battery. The bio-guided fractioning will help to find new promising molecules.
L-16
REGULATORY FRAMEWORK FOR THE DEVELOPMENT OF NATURAL PRODUCTS.
Rodríguez J
National Coordinator Center of Clinical Trials. 200 St. between 19 and 21 St., Atabey. Havana City. E-mail:
[email protected], Telephone: 271-83-31.
Background: The use of the natural medicine for the treatment of the different illnesses has increased their use
and importance in the international trade. In spite of this situation the data of security and effectiveness of a
number of plants, their extracts, their active principles and the finished products are really poor. The
requirements of quality, security and effectiveness that should complete these products for being marketed
under their medical use are settled down by Regulatory Agencies of Medicines (RMA). Objective: To obtain an
overview of the main regulatory approaches that governs the development of natural products. Methods: An
exhaustive bibliographical search was carried out using as key words: Regulation - Products - Natural. The
primary sources of information were: Web of the FDA, EMEA and IBERO-AMERICAN COUNTRIES, Web of the
World Organization of the Health (WHO) and Databases (MEDLINE, CENTRAL BIOMED and HINARI).
Results: As a result of the analysis of the information it was seen that the focus of the RMA regarding the
control of these products differs of a country to other, because in some the phytomedicines are very established,
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while in other they are considered as foods and it is not allowed to sustain that they possess therapeutic
qualities. Most of the underdeveloped countries have a great quantity of phytomedicines based on popular
knowledge and with big lacks of legislative approaches to establish these products of traditional use as
medications. Conclusions: Europe takes the leadership in the development of regulatory framework for the
development of the natural products. With some exceptions most of the regulations in existence is directed to
the control of herbal products and it doesn't stop the entirety of products conceived inside the natural medicine.
Cuba has two regulations for the use of its natural products.
L-17
UPDATE IN PRECLINICAL AND CLINICAL REGULATORY REQUIREMENTS IN CUBA.
Dr. Diadelis Remirez Figueredo.
National Centre of the State Quality Control of Drugs (CECMED).
E-mail: [email protected]
In the last decade there has been a global upsurge in the use of traditional medicine and complementary and
alternative medicine in both developed and developing countries. Pre-clinical and clinical assays are essential to
guarantee the efficacy and safety of natural products and are commonly requested by regulatory agencies to
approve marketing of these products.
This work describes important aspects about the art state of the regulatory status of herbal medicines in Cuba.
The objective is to give an updating about preclinical and clinical assessment of herbal medicines in Cuba.
The main toxicological assays and pharmacological activities to be tested in the evaluation of natural products
will be discussed in this presentation as well as the importance of clinical trials in order to guarantee the safety,
quality and efficacy of NHP, the main mistakes in Clinical Trials of natural products are explained.
The market and the main challenges are analysed in the investigation of the phytomedicines as well as the
tendencies in the growth of this attractive sector.
The natural health products are considered a very important source for the health in Cuba.
L-18
QUALITY MANAGEMENT SYSTEM FOR CLINICAL TRIALS WITH NATURAL PRODUCTS IN
CUBA.
Sandra Álvarez Guerra, S.
National Coordinator Center of Clinical Trials (CENCEC). 200th St. / 19 and 21 Atabey. Playa. Havana. E-mail:
[email protected], Telephone: 271-7397 ext. 107
The quality of the process of Clinical Trials carried out in the investigation to new products of natural origin
constitutes a factor that guarantees to promoters safety and reliable results which influences in obtaining the
registration. In Cuba, the National Coordinator Center of Clinical Trials offers services in the design and
conduction of Clinical Trials; among those we can mention researches with products of natural origin. One way
that guarantees process quality, is the implementation of a Quality Management System according to ISO 9001
standards. With this work we intended to describe the design and implementation of Quality Management
System for clinical trials in our institution. This system included stages of Preparation, Planning, Communication
and information, Application, Certification and Maintenance. There were two fundamental strategies: human
capital and documental system, both of supreme importance to guarantee the correct implementation. Some
tasks were executed that evidenced a significant advance in the personnel's formation, being reinforced the
performance of the leaders in the organization, the work in team, the identification with the objectives, the trust
and the mutual support, besides having a well structured documental system that supports all related to the
documentation which is generated during a clinical trial according to what establishes the reference standard.
The correct design and implementation of the quality management System allowed to reach a national and
international certification that brought harnessed multiple benefits contributing to a better development of the
clinical trials with natural products at national level.
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L-19
Presentacion Especial “Flordis Natural Medicines”, Australia.
Dr. Andrea Zangara
Flordis Natural Medicines - International Business Development Manager, Australia.
L-20
RESULTS ON PHARMACOLOGICAL RESEARCH TO VALIDATE TRADITIONAL MEDICINAL
PLANT USES IN TRAMIL PROGRAM: 1988-2008.
Morón F.
Medical Sciences University of Havana. Central Pharmacological Research Unit. Carvajal s/n entre Agua Dulce
y A, Cerro, Ciudad de la Habana, Cuba. [email protected]
Introduction: people use widely medicinal plants to solve primary health problems in Caribbean countries. Our
research unit has kept a close collaboration with TRAMIL (Regional Program for Applied Research and
Education on Medicinal Plant Uses in Caribbean Countries) since II TRAMIL Scientific Workshop was held in
Havana in 1988. We also have coordinated the Medicinal Plant Cuban Research Program (1988-2004) to
incorporate medicinal plants in our National Health System. Pharmacological and toxicological applied research
to validate traditional medicinal uses has some characteristics and challenges. The aim of this lecture is to
expose our experience during 20 years. Materials & Methods: it was reviewed our published results and those
included in TRAMIL´s Caribbean Herbal Pharmacopoeia during 1988-2008. Results: it is shown the results in
pharmacological and toxicological studies in 30 different species. Most of toxicological experiments indicated
that traditional uses were safe and a high number of them were validated in preclinical experimental models.
Conclusions: our data indicate that there is a high percentage of traditional medicinal plant uses which may be
supported with preclinical evaluation.
L-21
NATURAL PRODUCTS, SINCE PRE TO POSTMARKETING.
Jiménez G.
Pharmacoepidemiology Development Centre. 44 Street, No 502 esq. 5ta Ave Miramar Playa CP 11300 E-mail:
[email protected]
Plants have been the primary source of food and medicine for people of every culture throughout the world.
However, practitioners of traditional medicine need to be more aware of the problems of toxicity. Infrequent
adverse drug reactions will not be recognized without the existence of a formal system of reporting negative
experience. Physicians who have patients taking any particular medicinal plant should try to document negative
experiences, in order to gather enough scientific information about the adverse effects of the herb.
The renewed interest in the western world for plants used in traditional medicine, and the rapidly growing
interest in developing countries to start research programs in this area have, unfortunately, not emphasized the
great importance of taxonomic botany and documentation for such research. Often there is uncertainty about the
identity of plants reported to cause an adverse reaction. The World Health Organization (WHO) and the Uppsala
Monitoring Centre has established a project with the aim of attaining global standardization for herbal medicines.
On the other hand Cuba has a National Regulatory Authority with a defined policy about the regulation of
traditional medicine, which includes that information about the interactions between natural products and
conventional drugs should be reported. Moreover, Cuba has a Center in charge of Pharmacosurvelliance for the
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detection, assessment, understanding and prevention of adverse effects or any other possible drug related
problems. This conference shows the steps that Cuba follows in surveillance of natural products, same as the
program of WHO.
Key words: natural products, adverse effects, national surveillance system, natural product surveillance,
herbals products.
L-22
EL DESARROLLO DE ENSAYOS CLÍNICOS EN CUBA. EXPERIENCIAS CON PRODUCTOS
NATURALES. / Development of Clinical Trials in Cuba. Experiences with Natural Products.
Dra. María Amparo Pascual López, Directora, CENCEC, Cuba.
Resumen no disponible al momento de la Edición del Documento / Abstract not available in the moment of the
Edition of the Document.
L- 23
EL DESARROLLO DE PRODUCTOS NATURALES EN CUBA. EXPERIENCIAS DEL CENTRO DE
INVESTIGACION Y DESARROLLO DE MEDICAMENTOS. / Development of Natural Products in Cuba.
Experience of the Drug Research and Development Center.
MSc. Marlene Porto Verdecia, Directora General, CIDEM, Cuba.
Resumen no disponible al momento de la Edición del Documento / Abstract not available in the moment of the
Edition of the Document.
La medicina herbolaria es una de las formas más antiguas de atención médica que ha practicado la humanidad.
A través de la historia, se han utilizado las plantas en todas las culturas del mundo hasta nuestros días. En la
actualidad existe una extensa documentación científica relacionada con el uso de las plantas para la cura de
diversas patologías que van desde la indigestión hasta el cáncer y el VIH.
Se considera que las plantas medicinales son aquellas especies silvestres, cultivadas o manejadas que han
sido utilizadas por el Hombre en el tiempo al descubrir propiedades que ayudan al tratamiento o prevención de
una enfermedad o padecimiento. Su uso puede ser de forma tradicional, fundamentalmente en infusiones y
extracciones sencillas pero para lograr una correcta aplicación de la planta como droga se debe manejar y
conocer adecuadamente su hábitat y la cantidad y calidad de los principios activos que posee.
La Organización Mundial de la Salud hace notar que de los 119 fármacos derivados de las plantas, alrededor
del 74% se usan en la medicina moderna de manera que se correlacionan directamente con los usos
tradicionales que las culturas nativas les daban como hierbas medicinales.
Es interés de mostrar la experiencia alcanzada por el Centro de Investigación y Desarrollo de Medicamentos
(CIDEM) en el tema considerando el interés científico y técnico que se ha puesto en el uso de las plantas
medicinales lo que ha permitido desarrollar recomendaciones apropiadas para su uso a través de productos
fitoterapéuticos de calidad y seguros en su uso y que va desde su cultivo hasta su comercialización.
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COMUNICACIONES ORALES (CO) / ORAL COMMUNICATIONS (OC)
OC-01
FOOD AND DRUG INTERACTION IN PHARMACEUTICAL PRODUCTS OF NATURAL ORIGIN
Aznar E, González . R.
Centro Nacional de Biopreparados, Biocen. Bejucal, La Habana. Cuba.
Many substances of natural origin have been currently used as supplements or medicaments for prevention or
therapeutic purposes, with defined characteristics in terms of its composition, action mechanisms and indication.
These products give some protection against several diseases including micronutrient deficiency, (iron, zinc,
folic acid, and vitamins), endocrine and immune disease. A few. Nature has an arsenal of products with active
ingredients, which have been used to obtain the nutritional supplements, nutraceuticals, pharmaceuticals and
others. Has been little studied the influence of food on the effectiveness of some drugs and even less the
influence of drugs in the proper absorption and use by the body of nutrients that provide food. In this aspect of
particular importance for example prolonged treatments with drugs that damage the gastric mucosa and thus the
absorption of micro and macro nutrients, but are acquired through food or by the action of the drug itself is not
adequately, resulting in either case deficiency states.
Another example is the deficiencies in certain vitamins and other nutrients for abuse of laxatives. That's exactly
where it comes the use of natural products that often diminish the appearance of these interactions. The natural
elements can alter the pharmacokinetics of drugs through changes in absorption, as occurs with Trofin
(antianemic of natural origin) and iron salts, where the hem group is presented in its natural form, potentializing
by the gradient of amino acid that make this product better absorption and efficacy with minimal adverse effects
compared with iron salts. Our conclusion is that natural iron drugs (Trofin) as well as not develop interactions
with other drugs and foods, but, It is therefore important to know and continue to study possible interactions in
the new formulation, between drugs and food in order to obtain the desired therapeutic effect.
OC-02
ABSORPTION AND METABOLISM OF FLAVONOIDS AND THEIR METABOLITES
López R.
Pharmacy and Foods Institute. University of Havana. E-mail: [email protected]
Dietary flavonoids, a class of semi-essential food components, have long been believed to exert protective
effects against many diseases, in particular cardiovascular disease , cancer and neurodegenerative disorders.
The biological effect of these polyphenols and their in vivo circulating metabolites will ultimately depend on the
extent to which they associate with cells, either by interactions at the membrane or more importantly their
uptake. This has been well supported by a myriad of studies examining potential sites and modes of action. This
work summarises the current knowledge on the cellular uptake of flavonoids and their metabolites with particular
relevance to further intracellular metabolism and the generation of potential new bioactive forms. The impact of
membrane transporters as well as metabolic enzymes on the cellular availability of these phytochemicals is
examined.
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OC-03
PHARMACOLOGICAL INTERACTIONS OF TRADITIONAL MEDICINE PRODUCTS USED WITH
CONVENTIONAL DRUGS.
Rodeiro Guerra, I.
Department of Pharmacology, Center of Marine Bioproducts, Loma 37, Vedado, Havana City, Cuba.
Today, several herbals have been screened as phytopharmaceuticals and many patients with different diseases
take herbs in addition to conventional therapy. Between others, these patients are subjected to suffering the
effects derived from the co-administration of the herb and conventional drug, which is very frequently to found in
many cultures, including west world. Pharmacological interactions possibly derive from the modulation in
expression and/or activity of two major pharmacokinetic disposition systems, namely cytochrome P450 (CYP)
and the multidrug transporters where P-glycoprotein is identified as the major. Herbs used in the traditional
medicine and compounds isolated from its have been identified as substrates, inhibitors or inducer of different
cytochromes and/or transporters in humans. However, kknowledge about how natural products modulate its
enzymatic systems, which may lead to lower therapeutic efficacy and/or adverse effects of conventional drugs,
is limited. This works offers an overview of the knowledge about the interactions of these kinds of products and
the drugs. Interactions of ginkgo, saw palmetto, hipericum, kava kava, ginseng, between other are revised.
Effects observed after evaluation of different products used during several years in the traditional medicine in
Cuba (Mangifera indica L., Thalassia thestudium, Erythroxylum species) are presented.The elucidation of these
interactions may be important not only to predict possible undesirable effects deriving from the concomitant
intake of herbal constituents and conventional drugs, but also for further studies of positive uses of these
interactions as a way to increase the bioavailability of drugs that are P-gp substrates, as example. Physicians
must be prepared to discuss traditional medicine use with their patients and to be aware of potential interactions
without prejudices.
OC-04
SAFETY OF CURCUMA LONGA L. IN PATIENTS ASSUMING WARFARIN: A SERIES OF 12
PATIENTS
Gori L, Firenzuoli F, Mascherini V.
Clinical Center of Natural Medicine (Dir.: F. Firenzuoli, MD), S.Giuseppe Hospital, Az USL 11 Empoli, ITALY
Curcuma longa L extracts tritated in curcuminoids is a well known herbal based treatment for inflammatory
diseases particularly of the gastrointestinal tract. We often administer to our patients Curcuma longa extracts for
these types of pathologies and sometimes patients are contemporary assuming warfarin, a drug that has many
interactions not only with other drugs but also many foods. In a consecutive series of 12 patients assuming
different doses of warfarin and a stable INR, we adopted a protocol based on the administration for 2 weeks 500
mg of Curcuma longa extract tritrated at 95% in curcuminoids, and asking patients to underwent 3 controls of
INR, and then in the following 2 weeks assuming a dose of 1000 mg of Curcuma longa extract and asking to
repeat three controls of INR. In all patients INR remaind stable at the dose of 1000 mg too. We conclude
nevertheless a small series, probably Curcuma longa extracts do not interfere with warfarin.
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OC-05
PHYSIOLOGICALLY BASED PHARMACOKINETIC MODELING OF THEOPHYLLINE/ COFFEE
INTERACTION IN HUMAN
Law FCP1, Yao MC2, and Bi HC2.
1
Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, B.C. Canada.
V5A 1S6. E-mail: [email protected]
2
School of Pharmaceutical Sciences, Sun Yat-Sen University, 132 East Circle, University City Guangzhou,
Guangzhou, China, 510006.
Theophylline (TP) is a bronchodilator with a narrow margin of safety. Co-administration of TP and caffeine may
lead to an increased TP bioavailability in human due to competitive inhibition of drug metabolism involving the
hepatic CYP1A isozymes. In the present study, the physiologically based pharmacokinetic (PBPK) model of TP
was used to estimate an in vivo inhibition constant (Ki) of caffeine on TP metabolism and to predict the toxicity of
different TP dosing regimens in human with and without daily intakes of coffee and dietary caffeine. The PBPK
model was adapted from Ginsberg et al., (J. Toxicol. Environ. Health. 67: 297, 2004) and consisted of 5 flowlimited compartments including the liver, fat, richly perfused tissue, slowly perfused tissue, and blood. A modified
Michaelis-Menten equation was used to describe the competitive inhibition of TP metabolism in the liver. The
PBPK model was calibrated using the experimental data of Sato et al. (Eur. J. Clin. Pharmacol. 44: 295, 1993)
and found to simulate closely the kinetic data of caffeine-free adults after administering a single oral TP dose
(5.3 or 7.5 mg/kg). The PBPK model was optimized to obtain the “best-fit” Ki value based on the experimental
data of human drinking 2-7 cups of coffee daily. The model-optimized Ki value, 7 µg/ml was much smaller than
the 134 µg/ml Ki value reported previously using human liver microsomal preparations. The PBPK model also
was used to predict the risk of adverse reactions in adults after receiving multiple TP doses (340 or 450 mg
b.i.d.) with the usual intake of coffee and dietary caffeine. The simulation results showed that only the subjects
treated with the 450 mg dose had blood concentrations higher than the 20 µg/ml toxic TP level. The PBPK
model represents an innovative and useful approach of studying drug/coffee interaction in human.
OC-06
HERB-DRUG INTERACTIONS? DRUG COMPANY HYSTERIA OR COMMUNITY CONCERN?
Stough, C
National Institute of Complementary Medicine (NICM) Collaborative Research Centre in Neurocognition and
PO Box 218 Hawthorn, Victoria, 3122 AUSTRALIA e-mail: [email protected]
The incidence of consumption of herbal medicines throughout the western world is significantly increasing. In
response to this changing incidence has been the identification of potential herbal medicine-drug interactions.
Strong claims have been made in the popular press about the safety of herbal medicines and various
international governments and international health bodies have made statements relevant to this topic. In this
talk I discuss the hysteria behind this phenomenon. I outline the research that has been conducted in this area
and describe the evidence for potential problems and therefore the validity of current claims. I outline some
recent examples and describe a basic framework to evaluate the health risks of herbal medicine-drug
interactions in the future.
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OC-07
MOLECULAR MODELING OF CARVACROL (CONSTITUENT OF AROMATIC PLANTS) BY
MEANS OF MOLECULAR DYNAMICS SIMULATION.
Donnamaria, M.C 1, Xifreda C.C.2 and Grigera J.R 1
1
Instituto de Física de Líquidos y Sistemas Biológicos, IFLYSIB (CONICET- UNLP-CIC), C.C.565, 1900 La
Plata, Argentina, [email protected]
2
Laboratorio de Etnobotanica y Botanica Aplicada, LEBA ( FCE y Museo, UNLP), Argentina.
The use of natural antimicrobial systems for the preservation of foods is increasing. An example of a natural
preservative agent is carvacrol, a monoterpenoid phenol compound present in the essential oil fraction of
oregano and thyme, and in the essential oil of other numerous aromatic plants. Its low toxicity together with its
pleasant taste and smell suggests its use as a food additive to prevent bacterial contamination.The current work
is focused on conformational and hydration properties of carvacrol in aqueous solutions, modeled by molecular
dynamics simulation (MD), which has prove to be a highly reliable technique to predict properties of
biomolecules in solution. The MD simulations (GROMACS package) are performed at constant pressure and
temperature at normal conditions (P= 1bar,T= 300K) in nanoseconds time scale in aqueous solutions. Water
was modeled using the SPC/E model, while charges were optimized using Gaussian98 programme. DM results
show structural and dynamical preferences of carvacrol in water. The dihedral angles trajectories between the
ring and the side group reveal a relative flexibility of the molecule. The hydrogen bonds statistic shows the
existence of some internal hydrogen bonds that could play a role in stabilizing the molecular structure. This work
brings also interesting data regarding the influence of carvacrol on the water structure, disturbing the H bonding
pattern of SPC/E water as well as its dynamics. DMC and XCC are Researchers of Comisión de Investigaciones
Científicas de la Provincia de Buenos Aires-CIC, Argentina, and GJR is Researcher of CONICET, Argentina.
Authors gratefully acknowledge support of CONICET, CIC, UNLP. MCD and XCC also thank research personal
CIC subsidies.
OC-08
COMPUTATIONAL DISCOVERY OF NEW ANTHELMINTIC COMPOUNDS FROM AGAVE
BRITTONIANA TREL. SPP. BRACHYPUS
González-Castañeda Y, 1 Marrero-Ponce Y, 1 O. Guerra J, 2 M. Simonet A, 3 A. Macías F, 3 M. Nogueiras C, 4
Olazabal E5 and Serrano H5
1
Unit of Computer-Aided Molecular “Biosilico” Discovery and Bioinformatic Research (CAMD-BIR Unit), Faculty
of Chemistry-Pharmacy. Central University of Las Villas, Santa Clara, 54830, Villa Clara, Cuba.
[email protected]
2
Departamento de Licenciatura en Química, Facultad de Química y Farmacia, Universidad Central ‘‘Marta
Abreu’’ de Las Villas, Carretera a Camajuaní km 5.5, 54830 Santa Clara, Cuba
3
Grupo de Alelopatía, Departamento de Química Orgánica, Facultad de Ciencias, Universidad de Cádiz,
C/República Saharaui, s/n, 11510 Puerto Real, Cádiz, Spain
4
Departamento de Química Orgánica, Facultad de Química, Universidad de La Habana, C/Zapata s/n entre G y
Carlitos Aguirre, Vedado, Plaza de la Revolución, 10400, Ciudad de La Habana, Cuba
5
Centro de Bioactivos Químicos. Central University of Las Villas, Santa Clara, 54830, Villa Clara, Cuba.
Helminth infections are a medical problem in the world nowadays. In this report, bond-based 2D quadratic
indices, a novel TOMOCOMD-CARDD molecular descriptor and linear discriminant analysis (LDA) were used to
obtain a quantitative linear model -in accordance with the OECD principles for (Q)SAR validation- that
discriminates between anthelmintic and non-anthelmintic drug-like organic-compounds. The classification model
obtained classified correctly 87,46%, in the training set (352 chemicals). Furthermore, 165 compounds already
reported like anthelmintics were screened in a simulated virtual screening experiment (external-test validation).
As a result, it correctly classified 135 out of 165 (81.82%) of the chemicals, which were the most important
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criteria to validate the models. Another LDA model was carried out to outline some conclusions about the
possible modes of action of anthelmintic drugs. It has an accuracy of 83.69 % in the training set, and 87.41% in
the external prediction set. After that, the developed model was used in a virtual screening to predict the
biological activity of all the chemicals (19) that have been obtained by some of our research groups (Dr. Jose O.
Guerra) from Agave brittoniana Trel. spp. Brachypus, where several compounds (12) were identified by models
as anthelmintic. Finally, we have selected a group of 5 of them as theoretical most active chemicals to be tested
in an ¨in vitro¨ assay and one of them were evaluated ¨in vivo¨. As a result, the experimental observations
confirm theoretical predictions to a great extent as well as also show several candidates as fasciolicide drug-like
compounds.
OC-09
DISCOVERY OF NOVEL TYROSINASE INHIBITORS AS PROMISSORY DEPIGMENT DRUGS
FROM NATURAL SOURCES INTEGRATING THEORETICAL AND EXPERIMENTAL METHODS.
Le H1*, Casañola-Martín G1,2, Marrero-Ponce Y1,2,3, Mahmud K4,5
1
Unit of Computer-Aided Molecular ‘‘Biosilico’’ Discovery and Bioinformatic Research (CAMD-BIR Unit), Faculty
of Chemistry-Pharmacy, Central University of Las Villas, Santa Clara, 54830, Villa Clara, Cuba.
2
Institut Universitari de Ciència Molecular, Universitat de València, Edifici d'Instituts de Paterna, Poligon la
Coma s/n (detras de Canal Nou) P. O. Box 22085, E-46071 (València), Spain.
3
Unidad de Investigación de Diseño de Fármacos y Conectividad Molecular, Departamento de Química Física,
Facultad de Farmacia, Universitat de València, Spain.
4
Pharmacology Research Lab., Faculty of Pharmaceutical Sciences, University of Science and Technology,
Chittagong, Bangladesh.
5
Department of Pharmacology, Institute of Medical Biology, University of Tromso, Tromso 9037, Norway.
*
Corresponding author.
E-mail: [email protected]; [email protected]
The development of drugs from natural sources is an expensive process in time and finances terms. The mayor
impact in this process is always the identification of novel lead compounds once isolated. Recently, the
computational methods have become a suitable alternative to reduce the costly failures of drug candidates in
clinical trials by filtering virtual libraries of chemicals. It is known that the enzyme tyrosinase is election
pharmacological target in the treatment of skin hyperpigmentation. So the main purpose of this study is
developing QSAR (Quantitative Structure-Activity Relationship) models that can classify tyrosinase inhibitors
isolated from herbal plants from inactive ones for virtual identification of novel compounds. First a database of
658 organic compounds of great structural variation, 246 of which reported activity anti-tyrosinase is collected.
Every active and inactive molecule in the database is parameterized in atom-based linear descriptors by means
of the TOMOCOMD-CARDD program. K-means cluster analysis algorithms were used to obtain the training and
test series. Linear Discriminant Analysis was used to get classification functions. Twelve QSAR models of
tyrosinase inhibitory activity were achieved. Globally good classification of 93.51% was observed for best model
in the training set and 91.67% for the external prediction set. Finally, these fitted models were used in the
screening of new cycloartane compounds isolated from herbal plants. A good behavior is shown between the
theoretical and experimental results. The current report could help to shed some clues in the identification of
new tyrosinase inhibitors for entering in the pipeline of drug development.
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OC-10
PHARMACEUTICAL INFORMATION CENTER:
SPECIALIZED INFORMACION RESOURCES.
ITS
INCIDENCE
AS
A
PROVIDER
OF
Castro R.
Drug Research and Development Center (CIDEM). Ave 26 No. 1605 e/ Boyeros y Puentes Grandes. Plaza.
Ciudad de La Habana. CUBA. [email protected] / [email protected]
Pharmaceutical Information Center (CINFA) is an organization from the Drug Research and Development
Center (CIDEM) and the National Pharmaceutical Sector, whose mission is directed to the informative guarantee
of drugs research and development process in the country. Its interaction with entities and users of information
takes place through the access to Pharmaceutic Hall and Virtual Library, taking into account the generalization
in the use of new technologies of information and communication, the quantity and quality of available
informative resources and the immediacy of access and use of them.
The Pharmaceutical Information Center has reaffirmed its position as a provider of products and informative
services with a direct incidence on a consolidation of a pharmaceutical industry of excellency. In these terms, we
show details about its performance and about the pharmaceutic virtual library, which constitutes the result of a
systematic process of identification, selection and acquisition of diverse information resources, possibly
negotiable in an efficient and decentralized way. We also describe and characterize databases (digital
collections), which now constitute this virtual library, as well as the prospect in the design and development of
new available up-to-date resources.
We focus on different informative resources regarding medicinal plants and natural products, whose knowledge
and consultation bring about a great help in order to stimulate the investigations developed throughout the
country.
OC-11
SCORPION VENOM AS A NATURAL MEDICINE. IT’S USE IN MEDICINE.
Rodriguez JR, Pereira E.
Universidad Médica de Guantánamo Cuba (UMG) Calle 5 oeste entre 7 y 9 norte. Guantánamo. Cuba
Email: [email protected]
The Venoms have been interesting to the man across the human history, because they can kill us but even if we
use them in adequate doses they can be use as a medicine. Mineral, vegetables and animal venoms have been
used as a traditional medicine by peoples and the heritage about its use become us as a history of our
countries. The Pharmacology as Medical sciences have been studied venoms for two reasons: The venoms
action in the human body can be produce death, and the right use of venoms, could be use by treatment of
different illness. Scorpion venoms are including in the research feels of chemistry, biochemistry, pharmacology
and others medical sciences because they have a considerable number of molecules with biological action over
the cell of the human body and they are considerate one of a principal sources of molecules around the world. A
Historic and logical sequence about the use of scorpion Venom in medicine has been presented. It includes its
use in Cuba and around the world, a brief exposition about chemical composition and characterization of
scorpion venom from Cuba scorpion fauna and others countries has been made. A general overview about
cancer will be showing to understand the different mechanism of action of scorpion venom in cancer. The
theories about the way that scorpion’s venom use to attack malignant cells has been showed according to the
Philosophical point of view of the Natural medicine and Conventional (western) medicine.
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OC-12
PRE-CLINICAL STUDIES IN ANIMALS AND IN VITRO TO SHOW THE ACTIVITY OF THE
RHOPALURUS JUNCEUS SCORPION VENOM IN SOME CELL LINES OF CANCER.
ETHNOMEDICAL STUDIES ABOUT THE USE OF ESCOZUL.
Llorente S1, Poch E2.
1 Grupo
Provincial de Proyectos Sectorial Provincial de Salud Guantánamo. Asesor. Paseo esquina a Luz Caballero.
Guantánamo Cuba
2 Servicio de Proctología. Hospital general Docente Agusthino Neto Guantánamo Cuba.
The studies of the Rhopalurus junceus scorpion’s venom and its possible antitumor action started in the Medical
Sciences University of Guantanamo, Cuba at the 80’S. Chemical and basic toxicological studies have been
made and they showed the high toxicity over insects but the almost not toxicity over mammalians. Preclinical
studies in animal with natural malignant tumor showed the antitumor activity of a water solution of Rhopalurus
junceus scorpion’s venom, others studies made in the National Oncology and Radiotherapy Institute
demonstrated the citotoxicity activity over Erlich tumor, and adenocarcinoma and lymphoma cell lines, recently
studies caring out in the Immunology and Biological Product Center showed in vitro citotoxicity over the P3-X63
Y Dunning from myeloma and prostate cells. A General description of ethnomedical study about the natural
product, “home-made” and its use by the population of Guantanamo called Escozul, and the evidence of some
cases series to show the improving of quality of life and survival time of some patients will be presented.
OC-13
TITYUS SERRULATUS SCORPION VENOM: TOXINS, MECHANISMS OF ACTION AND
POSSIBLE MEDICINE APPLICATIONS
Souza, C.M.V.
Instituto Vital Brazil, Rua Maestro José Botelho No 64, Niterói, Rio de Janeiro, BRASIL.
E-mail: [email protected]; [email protected]
In Brazil human scorpionism sharply increased in the last ten years. The “yellow scorpion” Tityus serrulatus (TS)
is involved in most of severe and lethal cases. The venom of this specie is a complex pool of low molecular
basic proteins which acts mainly on ions channels resulting in transient but important disturbs on neuronal
electric conduction and massive release of neurotransmitters, inflammatory and immune modulators. Despite of
these, studies on TS toxins for medicine uses are not well developed than Cuban or Chinese scorpion species.
Recent proteomic approaches showed the presence of hyaluronidase, hypotensines and other active peptides in
TS venom. Machrophages immunomodulation and Nitric Oxide stimulation also has been described. Fields for
possible uses of this venom and toxins as pharmacological tools are of great interest. In this paper we present a
review of recent knowledge on TS venom this major action mechanisms, physiopathology and discuss the
possibilities of future research on its medicine application.
OC-14
EL ESCORPIN® CNH, UN COMPLEMENTO NATURAL BENEFICIOSO EN EL TRATAMIENTO DEL
CÁNCER DE PRÓSTATA.
Armendariz, AM.
Gerente General. A & A CITIC, Guadalajara, Jalisco. México.
Se muestra un reporte preliminar y básico de los principales beneficios experimentados en el estado de salud
en general de los pacientes atendidos en el A & A Centro de Información para el Tratamiento Integral del
Cáncer (CITIC) localizado en Guadalajara, Jalisco México, a los cuales se les administró el Escorpín® CNH,
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como complemento al tratamiento oncológico que realizaron para el cáncer de próstata. Se muestra la cantidad
de pacientes que han sido tratados, el tiempo de tratamiento y los principales beneficios reportados por los
pacientes. Después de analizar los datos se concluye que la utilización del Escorpín® CNH como complemento
en pacientes con cáncer de próstata puede ser útil para mejorar las condiciones de vida de los pacientes, así
como la tolerancia a los tratamientos oncológicos convencionales. Por lo que se recomienda su utilización
independientemente del estado del paciente, el tratamiento oncológico que el mismo este recibiendo y el tipo de
tumor de próstata que presente el paciente.
OC-15
THE USE OF NATURAL
HEPATOTOXICITY.
PRODUCTS
IN
CHRONIC
LIVER
DISEASE
AND
HERBAL
Zeki Yesilova
Gülhane Military Medical Academy, Division of Gastroenterology and Hepatology, Etlik, TR-06018 and Ankara,
Turkey E-mail: [email protected]
Chronic injury leading to fibrosis in the liver occurs in response to a variety of insults, including viral hepatitis
(especially hepatitis B and C), alcohol abuse, drugs, metabolic diseases due to overload of iron or copper,
autoimmune attack on hepatocytes or congenital abnormalities. Recent evidences have shown an increasing
demand of natural products, with proven hepatoprotective properties when used in the treatment of chronic liver
diseases. In-depth studies have proved Sylibum marianum to be anti-oxidative, anti-lipidperoxidative, antifibrotic,
and liver regenerative. Glycyrrhiza glabra has been shown to be hepatoprotective and capable of inducing an
indigenous interferon. Picrorhiza kurroa is proved to be anti-inflammatory, hepatoprotective and
immunomodulatory. Extensive studies on Phyllanthus amarus have confirmed this plant preparation as being
antiviral against hepatitis B and C viruses, hepatoprotective and immunomodulating. It has also been reported
that Cuban natural products (Mangifera indica L. (MSBE), Erythroxylum minutifolium, Erythroxylum confusum
and Thalassia testudinum) extracts are able to reduce damage of the rat hepatocytes induced by different
toxicants. On the other hand, herbal hepatotoxicity has been recognized for many years. Acute and/or chronic
liver damage occurred after ingestion of some Chinese herbs, herbals that contain pyrrolizidine alkaloids,
germander, greater celandine, kava, atractylis gummifera, callilepsis laureola, senna alkaloids, chaparral and
many others. Because histologic studies in cases of herbal hepatotoxicity are relatively few, the nature of liver
injury is not always known. In conclusion, licensing regulations and pharmacovigilance regarding herbal
products are still incomplete and clearcut proof of their efficacy in liver diseases is sparse.
OC-16
NATURAL PRODUCTS AS ALTERNATIVE TREATMENTS FOR METABOLIC BONE DISORDERS
Cagatay Oktenli
GATA Haydarpasa Training Hostipal, Division of Internal Medicine, Tibbiye Cad., TR-34668 and Istanbul, Turkey
Bone is a multifunctional tissue that consists of a structural framework of mineralized matrix and contains
osteocytes, osteoblasts, osteoclasts, chondrocytes, endothelial cells, immune and hemopoietic cells. Bone
remodeling, a continual process that remove old calcified matrix and regenerate new one synthesizing cells. The
cell responsible for bone resorption is osteoclast and the cell generating new bone is osteoblast. An imbalance
in the activity of osteoclasts and osteoblasts leads to adult skeletal defects including osteoporosis, Paget
disease, and osteolysis associated with periodontal diseases and multiple myeloma. Recently, the use of herbal,
non-mineral and non-vitamin supplements has expanded tremendously. To find natural products having a
stimulatory activity on osteoblast differentiation, several hundreds of plant extracts were screened, but only few
are subjected to the scientific evaluation and have been studied in animals or humans to prove their potency.
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Various traditional herbal formulae in Chinese and Ayurvedic medicine have demonstrable effects in
pharmacological models of osteoporosis. Potential usefulness of S. miltiorrhiza and the identified tanshinones
has been suggested in treatment of diseases involving bone loss. A methanol extract of the aerial parts of
Eclipta prostrata L. (Compositae) was found significantly to increase osteoblast differentiation. Increasing
observational epidemiological studies have also examined the linkage between dietary intake of phytoestrogens
and bone mass in humans and found soy protein or soy phytoestrogen intake beneficial in maintaining or
modestly improving bone mass. In conclusion, although our understanding of nutrients and other components
affecting bone health continues to grow, the process of acquiring knowledge is not over.
OC-17
ANTITHROMBOTIC POTENTIAL OF MEDICINAL PLANTS
García Ma, Armenteros DMa, Méndez BGb, González Rb, Dupeyrón Dc, Sanchez D Mc, Rodriguez. Dc,
Ramirez Rd, Alfonso MAa, Hernández Ja, Fleitas Aa, Díaz Aa, Mahia Ma, Piccinelli Ae, Rastrelli Le
a
National Institute of Angiology and Vascular Surgery .. 1551 Calzada del Cerro, Cerro, Havana City, Cuba. Email: [email protected]
b
Pharmaceutical Laboratory “Saul Delgado” , Havana City., Cuba.
c
Pharmaceutical Laboratory from Cerro, Havana City., Cuba.
d
Pharmaceutical Enterprise from Marianao, Havana City, Cuba
e
University of Salerno, Salerno, Italy.
The atherothrombotic diseases (myocardial infarction, stroke, lower limb thrombosis) are among the first causes
of death and disability around the World., therefore there is a need for preventive strategies Antiplatelet drugs
are prescribed for this purpose, however, the inter-individual differences with respect to the efficacy of the
available drugs makes the search for new therapeutic options a current matter of investigation. Some phenolyc
compounds isolated from plants inhibit platelet aggregation Therefore, the aim of the Group for Research on
Natural Products and Traditional Medicine of the National Institute of Angiology and Vascular Surgery of Cuba is
to characterize the potential antiplatelet agents among polyphenol-rich plants with traditional use. The plant
extracts were prepared from Citrus aurantifolia Ch., Morinda citrifolia L and Wendita calysina leaves, Citrus
sinensis L fruit peels and Zingiber officimale Rosc. rhizhome in 50 % ethanol. Ethanol was removed by rotoevaporation. Their antiplatelet effects were assessed by the turbidimetric method of platelet aggregation in
platelet-rich plasma (PRP). For “In vitro assays 285 µL of human PRP were incubated with 10 µl of an extract
by two minutes before the addition of ADP , collagen, epinephrine or arachidonic acid) as platelet agonists. The
“ex vivo “effects of plant extracts on platelet aggregation were evaluated by adding ADP to 400 µL of PRP
obtained from pretreated rats. The “in vitro” assays demonstrated a concentration –dependent inhibition of
platelet aggregation and differences among the extracts with respect to their effects against different platelet
agonist used...The “ex vivo” experiments have demonstrated that the “in vitro results are not “experimental
artifacts” and suggest that this effect may be clinically relevant. This is a confirmation that this research strategy
is useful for discovering of new antithrombotic agents from the Nature.
OC-18
DEVELOPING ANTI-INFLAMMATORY PHARMACEUTICAL
EXPERIENCE WITH ORGANIC SOURCED PUR 0110.
GRADE
BOTANICAL
DRUGS:
Dr André P. Boulet, PhD.
President & COO, PurGenesis Technologies Inc., Montmagny, Quebec, Canada.
Plants have been used as a source of medicine throughout history and even today serve as the basis for many
pharmaceuticals. As the pharmaceutical industry was maturing, it adopted the synthetic approach to drug
discovery with the vision that it could fulfill its needs for new drugs. This modem approach did not succeed in
providing new drugs to the pharmaceutical industry resulting in a growing market for botanical drugs. Indeed,
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Botanical drugs were worth $130,000 in 2008, but this segment is expected to reach more than $2 billion in
2013.
The FDA's guidelines, established in 2004, for the approval of prescription botanical drugs focuses on botanical
drug safety, efficacy and product consistency. Well designed clinical trials can address the FDA`s safety and
efficacy concerns. Product consistency of the complex collection of phytochemicals inherent to botanical
extracts remains a major challenge.
While FDA`s guidelines remain the backbone of any botanical drug development within the Botanical Drug
Industry, regulatory requirements difference between USA, Canada, Europe and the rest of the world are also
challenging when developing such new drugs.
PurGenesis Technologies Inc. is a Canadian privately owned company that develops and manufactures active
complex ingredients from natural sources. The core platform of PurGenesis’ research and development efforts is
a uniform and well-characterized molecular complex derived from green plants. This molecular complex, which
is characterized by a specific three-dimensional composition of pigments, proteins and lipids, is extracted from
certified organic plants. Traceability from the seed to the pills is under extensive QA & QC methods.
Characterization utilizing marker compounds, DNA fingerprint and bioresponse profile focusing on the integrated
biological response of cells to the entire collections of related compounds having multiple activities interacting for
a greater total activity.
Studies conducted by PurGenesis indicate that PUR0110 (an extract derived from this complex), prevents the
formation and noxious activity of Reactive Oxygen Species (ROS). ROS are a natural by-product of cellular
metabolism, but various disease processes as well as exposure to environmental stressor (UV radiation, heat
and pollution), can cause a dramatic increase in ROS levels, which can result in significant damage to cell
structures.
The efficacy of PUR0110 is based on its ability to capture noxious energy from all types of Reactive Oxygen
Species (ROS), including singlet oxygen, through its trapping system. While other antioxidants have the capacity
to capture noxious energy, their limited ability to dissipate this energy restricts the duration of effect. In contrast,
PUR0110 has the capacity to continue to dissipate this energy through its molecular complex providing an
unprecedented regenerating or turnover mode of action, which confers long-lasting antioxidant activity.
PUR0110 has also been demonstrated to have a strong anti-inflammatory effect. PurGenesis has conducted a
number of in vitro and in vivo studies illustrating specific actions of PUR0110 on the inflammatory process which
include Balancing inflammatory cytokines and Neutralizing harmful energy produced during inflammation.
These studies led to the exploration of PUR0110 in disease states such as psoriasis and ulcerative colitis, where
inflammation is thought to play a key role in the disease process. Indeed, the company is initiating its first in
human study with PUR 0110 as an enema formulation which will be following by a study in psoriasis patients in
the falls 2009.
Plants continue to serve as a valuable source of therapeutic compounds because of their vast biosynthetic
capacity and PurGenesis is dedicated to the extraction and development of such products.
OC-19
A RANDOMIZED CONTROLLED TRIAL OF VIUSID IN COMBINATION TO DIET AND EXERCISE
IN PATIENTS WITH NONALCOHOLIC STEATOHEPATITIS.
Dr. Eduardo Vilar*, Adelaida Rodríguez**, Bienvenido Gra**, Enrique Arús*, Raimundo Llanio****, Luis
Calzadilla*, Ali Yasells****, María del Rosario Abreu*****.
*Departamento de Hepatología, **Departamento de Nutrición *** Departamento de Patología, ****Departamento
de Gastroenterología, *****Departamento de Bioestadística.
Instituto Nacional de Gastroenterología, Habana, Cuba.
Nonalcoholic steatohepatitis is a significant health problem for which there is no universally accepted
pharmacologic treatment. Nowadays, lifestyle modification remains the gold standard to treat nonalcoholic
steatohepatitis. The combination of weight loss and antioxidant drugs to ameliorate insulin resistance and
improve steatosis, inflammation, and fibrosis, provide the rational for therapeutic trials. Aim: Our study
evaluated the efficacy and safety of viusid in association with diet and exercise for nonalcoholic steatohepatitis.
Methods: A randomized, controlled, and parallel-group trial was conduced in a tertiary care academic center
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(National Institute of Gastroenterology, Havana, Cuba). We randomly assigned 60 patients with liver biopsyproven steatohepatitis to 6 months of treatment with a hypocaloric diet plus aerobic exercise daily and 3 viusid
sachets daily or a hypocaloric diet and exercise. The clinical research network score with minor modifications
was used to compare the histologic changes as the primary end point. All biopsies were scored by a single
pathologist unaware to the patient clinical and biochemical data, treatment assigned and liver biopsy sequence.
Biochemical, metabolic, and anthropometric tests were secondary end points. Results: A significant
improvement in steatosis, necroinflammation, and fibrosis was seen in each group of treatment (P<0.01 for each
feature). The viusid group, as compared with control group, significantly reduced the mean total score (from 4.78
to 0.89 points in the viusid group versus 4.95 to 2.64 points in the control group [P=0.004]). On between groups
comparison, viusid was associated with a significantly greater improvement in steatosis (P<0.001), ballooning
(P=0.002), and lobular inflammation (P=0.025), but not fibrosis (P=0.25). Viusid was well tolerated.
Conclusions: Our results indicate that treatment with diet and exercise leads to a notable improvement in
histological features of nonalcoholic steatohepatitis; however, the administration of viusid intensifies the
improvements of histological findings, especially of steatosis and inflammation.
OC-20
INFLUENCE OF THE NUTRITIONAL SUPPLEMENT DIAMEL ON INDEXES HOMA - B AND
HOMA-IR IN PATIENTS WITH TYPE 2 DIABETES MELLITUS.
José A. Hernández Yero, David Vargas González, Raquel Jorge González
National Institute of Endocrinology, Hospital Pediátrico del Cerro.
Introduction: The nutricional supplement Diamel, can have a favourable influence in the diabetes type 2. This
supplement contains amino acids, vitamins, oligoelements and extracts of the lettuce and the bilberry, activated
by a process of the molecular magnetization, and acts at level of the pancreatic beta cells and the
gastrointestinal tractus. Objectives: To evaluate sensitivity to the insulin and the function of the beta cells with
the determination of the Homa-B and Homa-IR indexes, in patients with type 2 diabetes that received treatment
with glibenclamide more Diamel. Design and methods of the investigation: A total 60 patients with type 2
diabetes was selected at random to administer Diamel plus glibenclamide (group A, n=30) and glibenclamide
alone, group control, (group B, n=30). Two capsules of Diamel were administered 30 minutes before the
breakfast, lunch and the supper. The patients including were between 40 and 65 years of the age and had 0 to
10 years of history of the diabetes. Those patients with the greater HbA1c from 10% or with moderate or severe
chronic complications were excluded. The patients were followed during 6 months with basal determinations of
insulinemia and glucemia, as well as glucemia post-prandiales of two hours and HbA1ç and determinations of
the Homa-B indixes and Homa-IR to the beginning, to the 3 and 6 months. Results: The patients of the group A
and group B presented to similar characteristic clinicals at the beginning and received identical
recommendations about nutrition and physical activity. The levels of the glicemic control, measured by fast blood
glucose and post-prandial blood glucose and the HbA1c displayed a significant diminution in the group that used
the Diamel. The correlation between fast blood glucose and cells beta function expressed at HOMA - B was
positive in both groups at the beginning of the study but it was more significant positive mind in the group A
To that in group B, to the 6 months of the pursuit. The relation with the HbA1c was a weak positive
correlation to the 6 months in group B whereas the group with the Diamel these results were significant. The
correlations between the HOMA - IR with the BMI and the abdominal circumference and waist/hip index
remained positives in both groups, but was more consistent statistically and significative in group A.
Conclusions: The nutricional suplement was useful in to increase the function of the beta cells in patients with
type 2 diabetes during the 6 months of the pursuit. The anthropometric variables BMI, waist circumference and
index waist / hip presented a correlation significant with the index Homa-IR with the use of the Diamel.
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OC-21
UTILIZATION OF MARINE BACTERIA FOR PRODUCTION OF NATURAL PRODUCTS.
Núñez RR, Ortiz E, Oramas JA, Fonseca EL, Paneque K, Martínez C, Caballero V, Barbán O, Díaz Y,
Morales M.
Centro de Bioproductos Marinos (CEBIMAR), AMA, CITMA, Loma y 37, Alturas del Vedado, C. Habana, CP
10600, CUBA, Tlf. 881 1298 y 881 9300.
E-mail: [email protected] y [email protected]
Since 1985, the Microbiology Department of CEBIMAR have isolated marine microorganisms from sea water,
the objective of this, was to create a Marine Bacteria Collection (CBM) with biotechnology potentialities. These
microorganisms had been classified on basis of capacity of heterotrophic bacteria to producing different
substances from simple media during their metabolism. The CBM contain more than 400 strains with capacity to
produce important metabolites like as antitumor activities, antimicrobial activities, surfactants, etc. In this
collection more than 120 strains are capable to use hydrocarbons as unique carbon and energy source, with this
potentialities were obtained two bioproducts at industrial scale, called IDO-503 and BIOIL-FC. The first one was
used in microbial enhance oil recovery in the Boca de Jaruco oil field and was capable to recover more than 10
000 ton of crude oil. BIOIL-FC was developed against oil pollution and was used since 1992 with excellent
results at different ecosystems like mangroves, beaches, bays, rivers and soil. The oxidation of all fractions of oil
was significative in all of cases more than 85 %.
We develop, at pilot scale, some antimicrobial substances used against different pathogenic agents, in order to
obtain standardized products that will be used for increasing the quality of live.
OC-22
PHARMACOLOGICAL CHARACTERIZATION OF BIOACTIVE COMPOUNDS FROM THE SEA
ANEMONE EPICYSTIS CRUCIFER.
Garateix A1, Salceda E2, López O2, Rodríguez A1, Standker L3, Zaharenko AJ4, Richardson M5, de Freitas
JC4 and E. Soto2
1
Centro de Bioproductos Marinos, CITMA, La Habana, Cuba. 2Instituto de Fisiología, Universidad Autónoma de
Puebla, México. 3IPF PharmaCeuticals GmbH, Hannover, Germany. 4Instituto de Biociências, Universidade de
São Paulo, Brasil. 5FUNED, Belo Horizonte-MG, Brasil.
Sea anemones represent a valuable source of different biologically active compounds, being the Na+ and K+
channel toxins the most known. In this work we studied chromatographic fractions of high and intermediate
purity, obtained from the sea anemone Epicystis crucifer, on voltage- and ligand-activated currents. The
biological evaluations were done in dorsal root ganglion (DRGs) cells in culture and in fresh isolated cells of the
snail Helix aspersa, using whole cell patch clamp techniques. The evaluation of Sephadex G-50 fractions
showed Na+, K+ and acid-sensing ionic channels (ASICs) channel blocking actions in fraction III and
antiglutamatergic action in fraction II, respectively. Fraction II and III were submitted to further purification steps
comprising ion exchange and reversed-phase chromatography. A peptidic compound (MW 5297.88 Da
according to MALDI-TOF measurement) was obtained from Fraction II, which blocked glutamate (1 mM)
currents in isolated snails neurons (IC50=7.36 ± 3.9 µM, n=15 ). Also, another new peptidic compound was
obtained from fraction III (MW 3475 Da according to MALDI-TOF measurement). In preliminary experiments, it is
shown that this peptide inhibited ASICs currents on DRG cells in the micromolar range (n=5). The
characterization of these peptides on other currents is now in progress. These findings support the presence of
Na+ and K+ channel toxins as well as novel glutamate and ASICs inhibitors in this sea anemone that should be
further characterized because of their potential therapeutic interest.
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OC-23
ANTIOXIDANT AND CITOPROTECTIVE EFFECTS OF BM-21, AN AQUEOUS ETHANOL
EXTRACT FROM THALASSIA TESTUDINUM IN VARIOUS EXPERIMENTAL MODELS.
Menéndez R, Garateix A, García T, Morales R, Marrero G, Rodeiro I, Laguna A, Regalado E, Fernández
MD.
Centro de Bioproductos Marinos. Agencia de Medio Ambiente. La Habana. Cuba.
BM-21, the standardized extract obtained from the sea grass T. testudinum, a marine plant highly abundant in
Cuban coasts, was evaluated for its antioxidant and citoprotective effects in experimental models. The plant
extracts showed free radical scavenger effects on OH*, ABTS+, O*- and peroxilic radicals, as well as inhibitory
effects on “in vitro” spontaneous and metal-induced lipid peroxidation in brain and liver membranes,
respectively. Also, pretreatment with BM-21 (24h, 5 - 100µg.ml-1) prevented the tert-butyl hydroperoxide-induced
cell damage (IC50 190 µg.mL-1) the rise in malondialdehide (MDA) and the significant depletion of reduced
glutathione (GSH) of freshly isolated rat hepatocytes. Accordingly, BM-21 exhibited remarkable hepatoprotective
activity “in vivo” against acetaminophen-induced hepatotoxicity (1.5 g.kg-1) in mice after single and repeated oral
administration (100 mg.kg-1). Besides, repeated oral administration (100 and 400 mg.kg-1) prevented death rate
and neurological symptoms after unilateral carotid occlusion in Mongolian gerbils and decreased
malondialdehide content in whole brain at the same doses. Oral administration of BM-21 (400 mg.kg-1)
prevented toxicity induced by the subcutaneous administration of achrylamide (70 mg.kg-1). At the same doses,
the extract restored stress oxidative parameters in sciatic nerve, liver and whole brain homogenates. In
conclusion, this study is the first to demonstrated the citoproof T. testudinum extract in experimental models.
The key finding of the present study in that BM-21 has central and peripheral neurorpotective effects. Although
further investigations ate necessary to identify the effective citoprotective compounds our present results
suggest that BM-21 contain selective antioxidants that could be useful in preventing cell damage caused by
oxidative stress.
OC-24
NEW COMPOUNDS OBTAINED
PHARMACOLOGICAL ACTIVITY
FROM
SPONGES
FROM
CUBAN
COASTS
WITH
Erik Regalado, Abilio Laguna, Olivie Thomas*
Centre of Marine Bioproducts, Loma & 37, Vedado, PC 10600, Havana City, Cuba; e-mail:
[email protected]
*Laboratory of Chemistry of Bioactive Molecules and Fragrances, University of Nice-Sophia Antipolis, Faculty of
Sciences, Nice, France.
The exploration of the marine environment as a potential source of bioactive natural products with original
structures is in full expansion. Nowadays, there are only four marine-derived products currently available:
vidarabine (antiviral), cytarabine (antitumor), cephalosporine (antibiotic) and (Yondelis®, antitumor).
Furthermore, more than 15 new compounds isolated from ascidians, seaweeds, bryozoans, or sponges are now
under clinical trials for drug development, mainly for anticancer therapy. Prialt™, an omega conotoxin isolated
from Conus magus, is the first drug of marine origin to reach the FDA approvable status, and is the most
powerful painkiller known to date.
In recent years, several compounds has been obtained from sponges from all over the world that shows
promissory antitumor activity and our group decided to search for new bioactive substances with
pharmacological activity from sponges that grows in Cuban coasts. These studies were aimed at developing a
standardized approach to characterize the marine chemo-diversity in a model community. Several sponges were
studied and those that it extracts shows antitumor activity were studied in order to isolate its main components.
From Agelas sp. were obtained 5 bromopyrrole alkaloids and from Niphates sp. were obtained 6 nucleosides
that never have been previously described from a marine organism.
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OC-25
AN INVESTIGATION OF POTENTIAL USES FOR AMPHITOXIN, A PYRIDINIUM ALKALOID FROM
THE JAMAICAN SPONGE, AMPHIMEDON COMPRESSA.
Thompson M.1, Gallimore W.1*, Townsend M.2, Chambers N.2, Williams L.3
1
The University of the West Indies, Mona Campus, Kingston 7, Jamaica, West Indies, (phone: 876 927 1910;
fax: 876 977 1835).
2
The Rodent Biology and Control Unit, Food Storage and Prevention of Infestation Division, Ministry of Industry,
Technology, Energy and Commerce, 15 Gordon Town Road, Kingston 6, Jamaica
3
The Scientific Research Council, P.O. Box 350, Hope Gardens, Kingston 6, Jamaica
*Author to whom correspondence should be addressed
Tel: (876) 927 1910 Fax: (876) 977 1835 E-mail: [email protected]
The fierce competition for space on the coral reef has been thought to be one of the reasons why marine plants
and animals produce secondary metabolites which can deter, debunk or even destroy potential predators.
In our continuing investigations to determine if some of the compounds produced by these marine organisms
can possess industrial applications, the common reef sponge Amphimedon compressa was collected by SCUBA
in Port Royal, Jamaica, lyophilized and extracted with MeOH:CH2Cl2 (1:1) at room temperature. The crude
extract, when concentrated under reduced pressure, yielded a red-brown pasty residue which, on
chromatography with Sephadex LH-20, afforded the pyridinium alkaloid amphitoxin as its major constituent, the
structure of which was elucidated by interpretation of spectroscopic data from 1H and 13C NMR experiments. The
crude extract and the fractions containing high percentages of the metabolite were assessed for ichthyotoxic
activity over a three hour period using the Xiphophorus variatus (moon fish) in which note was taken of the rate
and manner of swimming activity as well as mortality in the organism. Insecticidal activity with the agriculturally
significant pest Cylas formicarius elegantulus (Sweet potato weevil) was examined to determine the potential of
the extract for an agricultural application. The extracts were dissolved in acetone and topically applied to the
dorsal part of the insects. The ability of the extract to cause mortality in the rodent, Rattus norvegicus (Norway
rat), was examined with fifteen randomly selected rats who were given varying concentrations of aqueous
solutions of the fractions and the crude extract.
From the experiments conducted, moderate insecticidal activity was observed while toxicity of the fish was
evidenced by the high mortality rates in all the fractions tested. The species of rodent examined did not appear
to be adversely affected by the metabolite under study.
OC-26
USEFUL ANTITUMOR MARINE COMPOUNDS
Bhardwaj S1, Geol R2
1
Department of Pharmacology Seth G.L Bihani S.D College of Technical Education Sriganganagar Rajasthan,
India 335001. E- mail-: [email protected]
2
Department of Pharmacology Pharmaceutical Science and Drug Research, Punjabi University Patiala.
It has become obvious that the marine environment produces chemical compounds that have significant
activities against mammalian cell. Perhaps the first notable discovery of biologically active compounds from
marine source was reported from 1950 to 1956 by Bergman et al on two compounds Spogouridine,
spongothymidine.
Introduction: There are significant number of compounds available from marine source presently on clinical
/preclinical trials as anticancer agents. Although currently there are no approved agent that have come directly
from the marine environment. It was realized that marine environment should produce a very large number of
exquisitely potent compounds, a probable reason for the presence of extremely active chemo types that the
dilution effect of see water means that any toxins must be very potent , as it usually has to cross a finite
distance to deter any predator.
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Name
Didemnin B
Bryostatin I
Dolastatin 10
cematodin
Ecteinascidin 743
Source
Trididemnum Solidum
Bugulo Neritina
Dolabella (marine microbe)
Synthatic derivative of Dolastatin 15
(marine microbe)
Ecteinascidia turbinata
status
Phase II CANCER
Phase II CANCER
Phase II CANCER
Phase II CANCER
Phase IIICANCER
Materials and Method: Didemnin B was isolated by Rinehart’s group from extract made of the tunicate
Trididemnum solidum that demonstrated a potent antiviral and cytotoxic activity against p388 and 1210 murine
Lukemia cell line. Another agent dolastatins are a series of cytotoxic peptides that were originally isolated by
pettit’s group from Dolabella auricularia .as a result of synthetic processes many derivatives of Dolastatins have
been synthesized. Conclusion: Marine environment produces chemical compounds having significant anti
tumor activity. These compounds are present on clinical trials and need to be further exploration.
OC-27
IMPACT ON CUBA NEONATAL MORTALITY WITH THE USE OF A NATURAL SURFACTANT.
FUTURE NEW INDICATIONS
Fernández-Limia O, Díaz E.
National Centre for Animal and Plant Health (CENSA). Carretera de Jamaica y Autopista Nacional, San José de
las Lajas, La Habana, Cuba. E-mail: [email protected]
Since the beginning of the 1980s, the application of natural first and synthetic surfactants after, either
therapeutic or prophylactic has had a major impact in reducing infant mortality by Newborn Respiratory Distress
Syndrome (NRDS) formerly called Hyaline Membrane Syndrome which is observed in premature infants and is
caused by lung immaturity with a deficit of endogenous surfactant. Due to difficulties in acquiring these drugs, in
part because of its high price and mostly by the blockade that is subject the country and at neonatologist’s
request, for over 10 years there has been a natural surfactant Surfacen of porcine origin produced by our center.
Production has been increased so that now fully meets the needs of the country. This, together with the artificial
ventilatory facilities, has been determined a reduction in infant mortality by SDRN of 0.8 to 0.2 per 1000 live
births. Currently under clinical efficacy evaluation in other conditions such as the Acute Respiratory Distress
Syndrome (ARDS) that occurs at any age after birth for many reasons that cause lung inflammation and
transudate of protein and other elements of the blood which inactivate the surfactant system causing alveolar
collapse and reduced gas exchange due to the, anti-inflammatory, antimicrobial and antioxidant properties that
Surfacen has. In addition there a number of questions as the effect of long-term complications, the method of
administration, combined with new maneuvers, its prophylactic use in SDRN and continue the search for new
ventilation surfactants with greater efficacy and lower cost.
OC-28
IRON BIOAVAILABILITY IN THE PRODUCT LINE OF NATURAL ORIGIN TROFIN: USE OF THE
RADIOISOTÓPICA DOUBLE TECHNIQUE.
González H. R.1, Pizarro F.* y Aznar E.
1
National Center for Biopreparados, Biocen, Havana, Cuba.
*Institute of Nutrition and Food Technology (INTA), Chile University.
[email protected]; [email protected] & [email protected]
Introduction: Iron deficiency in one of the most prevalent nutritional deficiencies in the world. Infants,
preschoolers, schoolchildren, women of childbearing age and pregnant women are more vulnerable to develop
this shortcoming. This justifies the development of new products of natural origin, such as the production line
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Trofin based on iron hemin, developed by the National Center for Biopreparados. Objectives: The purpose of
this study was to determine the bioavailability of iron in three of these products: Trofin liquid, Trofin dust
(dried) and Trofin tablets, by using the double radioisotope technique with Fe55 and Fe59. Materials and
Methods: The study was conducted at the Institute of Nutrition and Food Technology from Chile University and
included 15 people who had indicators of iron metabolism within normal limits. The product Trofin was given
orally and in single-dose in the presentations: liquid, dehydrated and tablets marked with isotopes Fe55 and Fe59
in accordance with established international standards. For 15 days the radioactivity was determined for each of
the isotopes in the blood of individual subjects and the bioavailability was determined for each of the present
study. Results: The results of the bioavailability were adjusted to different concentrations of serum ferritin found
that for patients with 20 ug/L of serum ferritin the bioavailability was 28.9% for patients receiving Trofin liquid,
24.8% for those receiving Trofin dust and 25.4% for those receiving Trofin tablets. Conclusions: We concluded
that the three preparations tested have a high bioavailability.
OC-29
ANTIVIRAL ACTIVITY OF PHYLLANTHUS ORBICULARIS HBK AGAINST ENTEROVIRUSES
Acosta M1, Álvarez AL1, Sarmientos L2, Fonseca M2, Resik S2, del Barrio G1*.
1
Grupo de Antivirales Naturales. Facultad de Biología. Universidad de La Habana. Calle 25 No.455 e/ J e I,
Vedado, Plaza de la Revolución, Ciudad de La Habana, Cuba.
2
Laboratorio de Enterovirus. Instituto de Medicina Tropical Pedro Kourí. Autopista Novia del Mediodía, Km 6 ½,
La Lisa, Ciudad de La Habana, Cuba. *[email protected]
The antiviral activity of Phyllanthus orbicularis against herpesviruses and hepatotropic viruses has extensively
been investigated. In this work, the inhibitory activity of the aqueous extract from this Cuban endemic plant was
evaluated against Poliovirus 1, Coxsackievirus B5 and Echovirus 9. Citotoxicity of the test extract was assessed
by means of the MTT colorimetric assay and antiviral activity was determined as a function of viral-induced
cytopathic effect (CPE) reduction under a light microscope. The time of inhibition was studied using a time-ofaddition experiment and virucidal assays were carried out in order to assess the extracellular virus-inactivating
capacity of the plant extract. P. orbicularis inhibited the in vitro propagation of all tested viruses. CE50 values
ranged from 115 to 130 µg/mL. Selectivity indexes (SI) recorded for this extract was greater than 9. An
extracellular virucidal activity was found for this plant since viral suspensions titres decreased by 1 log10 or more
after treatment with the extract. P. orbicularis was effective against all studied enteroviruses. No previously
works are available in literature describing anti enteroviral activity from this species.
OC-30
NEW FLAVONOL WITH ANTI-INFLAMMATORY ACTIVITY FROM BOLDOA PURPURASCENS,
CAV
Dulce María González Mosquera1, Amuri Kilonda2, Suzanne Toppet2, Frans Compernolle2, Wim Dehaen2,
Sandra Apers3, Luc Pieters3, Armando Cuéllar Cuéllar4 , Liliana Vicet Muro1 and Yannarys Hernández
Ortega1
1
Department of Pharmacy at Marta Abreu University, Road to Camajuani Km 5, Santa Clara, Cuba.
2
Molecular Design and Synthesis, K.U.Leuven, Celestijnenlaan 200F, 3001 Leuven-Heverlee, Belgium.
3
Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium.
4
Institute for Pharmacy and Food (IFAL), University of Havana, Ave. 23, 21425, Lisa, C. Habana, Cuba.
Boldoa purpurascens, Cav. it plants belonging to the family Nyctaginaceae, it has shown a powerful diuretic
activity in tests preclinical, with similar effect to the furosemide.
Phytochemical analysis of the leaves of Boldoa purpurascens Cav[1]. led to the isolation of one flavonol
glycoside [2,3]. This product is new compound. This structure have been determined by mass spectrometry and
by 1D and 2D NMR analysis, i.e., 4’,5-dihydroxy-6,7-methylenedioxyflavonol-3-O-α-L-rhamnopyranosyl-(1→2)β-D-xylopyranoside[4]. The aglycone 3,4’,5-dihydroxy-6,7-methylenedioxyflavonol is known as gomphrenol[5]. The
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new flavonoid was evaluated to know its effects in the acute and chronicle phases of the inflammation. For that
reason, two experimental techniques were developed: edema to plant induced by dextrano and granulomas
induced by cotton disks. In the experimental test doses of 2.5, 5.0 and 10 mg/Kg of weight were used , the
volume of administration of 10 mL/Kg; as positive control a solution of Indometacine was used (7 and 5 mg/Kg)
and negative control water distilled. The gonfrenol-3-O - [ α- L-rhamnosil-(1→2) - β- D-xilósido] presented antiinflammatory activity in the acute phase to superior dose to 2.5 mg/Kg in the experimental pattern of edema to
plant induced by dextrano; also, this activity increased when increasing the dose. In the granulomas pattern
induced by cotton disks, the compound presented similar anti-inflammatory activity to the Indometacine to all the
evaluated doses, being bigger the effect to the 10 mg/Kg dose. The statistical analysis was carried out by the
test of Kruskal-Wallis with an interval of trust of 99% and a level of significance of 0.816. The flavonol; showed
significant dose-dependent inhibition of both acute and chronic inflammation. The activity was comparable to
that of the standard reference drug, indometacine The results of the present investigation indicated that the
flavonol isolated of B purpurascens, Cav. shows profound antiinflammatory activity
Keywords: Boldoa purpurascens, Nyctaginaceae, flavonol glycosides, gomphrenol glycosides.
Reference:
1-Roig, J. T, 1988. Dictionary of Cuban Common Yams, 3rd. Editorial Science and Technic, pp 225-226. 2Niassy, B.; Um, B. - H, et al to the, 2004. C. R. Chimie 7, 993-996. 3-Jpn. Kokai Tokkyo Koho, 1988,
JP63203682 A2. 4-Magalhães AF, Tozzi AM, et al to the, 2007 Acad Bras Cienc; 79:351-675-Bouillant ML,
Redolfi P, et at the 1978. Phytochemistry; 17: 2138-40.
OC-31
NEW APPROACH OF R. MANGLE EFFICACY IN EXPERIMENTAL PEPTIC ULCERS.
Luz María Sánchez Pereraa, Dulcer María Solerb; Betty Manceboa; Ivis Fragaa; Roberto Faurea.
a
Chemistry, Pharmacology and Toxicology Group. National Center of Animal and Plant Health, CENSA. Adpo.
10, CP 32 700, San José de las Lajas, Cuba.
b
Development Group. Production’s Direction. National Center of Animal and Plant Health, CENSA. Adpo. 10, CP
32 700, San José de las Lajas, Cuba.
Introduction: Rhizophora mangle L. has a potential activity against experimental models of acute and chronic
gastric ulcers (Sánchez, 2001; 2004; Beringuer, 2006). We were studied another pharmacological test as part of
pharmacological II. The not toxic effect was shown in acute and genotoxicological trials. In the present work we
described other pharmacological evaluation and chemistry-pharmacology test in the development from a new
drug by the treatment of gastroduodenal ulcers. We were proceeding to formulate tablets since laboratory to
industrial scale with dried aqueous extract from barks of this plant. We studied the stability of intermediate
product and final formulation. We validate the analytical method by CLAR to determinate a mark in tablets as
quality indicator in self. Some tests were made to evaluate the gut absorption of active extract and tablets and
we determinate the digestibility of protein in presence of the active extract. It was possible to formulate tablet by
directed compression in lab and industrial scales. Intermediate product and tablets were stable by 1 year in
environmental condition with good quality parameters (technology, chemistry and biological activity). Dried
aqueous extract has high gut absorption and this extract not affect the proteins digestibility. The dissolution of
tablets was 30-70 % in different dissolution medium.
References: 1. Sánchez LM et al (2001). Journal of Ethnopharmacology 77, 1-3. 2. Sánchez LM et al. (2004).
Pharmaceutical Biology 42 (3). 3. Sánchez LM et. Al. (2006) Journal of Herbal Pharmacotherapy 6(5). 4.
Beringuer Bettina, Sánchez LM et al. (2006) Journal of Ethnopharmacology 103(2) pp. 194-200.
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OC-32
CUBAN EXTRACT FROM STEM BARK OF MANGIFERA INDICA L (VIMANG):
FROM
PRECLINICAL STUDIES TO THE BEST CLINICAL INVESTIGATION STRATEGY: THE
PERMANENT CHALLENGE FOR THE PHARMACOLOGY OF NATURAL PRODUCTS.
Delgado R
Scientific Director and Clinical Pharmacology Department, National Centre of Clinical Trials, Havana, Cuba.
Since the origin of the man’s existence, plants and other natural sources have been used with medicinal
purposes.
In Cuba, during several years, aqueous extract from stem bark of Mangifera indica L. was obtained and used
during a lot of time by empiric practitioners as nutritional supplement for the treatment of diverse affections.
The extract was studied by Centre of Pharmaceutical Chemistry in order to development and standardize
pharmaceuticals production and for these purposes were carried out different projects and investigations with
the objective to get a complete phytochemical, analytical, toxicological and pharmacological characterization
according to international and Cuban regulatory requirements for using phytomedicine products.
Few times, in the investigation of medicinal plants in our country, the preclinical evaluation of a natural product is
studied in details. These studies have been published in scientific journals with high impact factor. Approximately
15 years, we have been working with this extract, demonstrating antioxidant, anti-inflammatory, analgesic and
immunomodulatory activities. The institution began the development of a strategy of clinical evaluation, in order
to demonstrate the therapeutic effectiveness of the extract in diverse pharmaceutical forms through controlled
clinical trials in diseases with an important inflammatory or/and oxidative stress component that guarantee the
introduction of Vimang® like phytomedicine.
Up to now, the clinical trials carried out have not shown conclusive results and this is the main challenge of the
natural products: To demonstrate their true clinical effectiveness, in spite of they show excellent preclinical
activity. In order to confirm it, in the clinical trials, is necessary to dose adjustments, to identify the best time of
administration, chronopharmacological determinations, and other pharmacological strategies with the objective
to find the best way for using these products in the clinical practice. After the conclusion of preclinical phase,
there are many aspects for clarifying in the near future, until to reach the true clinical effectiveness of the
products like a standardized phytomedicine. The biggest challenge, therefore, we have not even reached and
this is to introduce this phytomedicine in the clinical practice for specific therapeutic indications.
REFERENCES: Int Immunopharmacol 2 (6): 797-806, 2002. / Phytother Res 17 (10): 1182-1187, 2003. /
Phytother Res 17 (10): 1203-1208, 2003. / Pharmacol Res 50(2):143-149, 2004. / Pharmacol Res 50(2): 165172, 2004. / Int Immunopharmacol 4(8): 991-1003, 2004. / Pharmacol Res 51: 427-435, 2005. / Arch Biochem
Biophys. 439(2):184-93, 2005. / Drug Metabol Drug Interact. 21(1): 19-29, 2005. / Phytother Res 19(3): 211-5,
2005. / Eur J Pharmacol 513(1-2): 47-55, 2005. / Arch Med Res 37: 158-164, 2006. / Phytother Res 20: 120124, 2006. / Pharmacol Res 53(3): 253-260, 2006. / Chemico-Biol Interact 159: 141-148, 2006. / J Pharm
Pharmacol 58(3): 382-92, 2006. / Int Immunopharmacol 6(9): 1515-1523, 2006. / Int Immunopharmacol 6(9):
1496-505, 2006. / Phytomedicine 13(6): 412-8, 2006. / Food Chem Toxicol. 44(10): 1707-13, 2006. / Biochim
Biophys Acta 1760(9):1333-42, 2006. / Eur J Pharmacol 547(1-3): 31-36, 2006. / Pharmacol Res 54(5):389-395,
2006. / J Pharmacol Exp Ther 320(2):646-53, 2006. / Pharmacol Res 55(2):167-73, 2007. / Pharmacol Res.
55(5):351-8, 2007. / Food Chemistry 107(3): 1008-1014, 2008. / Pharmacol Res. 57(1):79-86, 2008. / Chem
Biol Interact. 172 (1): 1-10, 2008.
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OC-33
REGULATORY T CELLS INDUCTION AS A MECHANISM OF ACTION FOR NATURAL
PRODUCTS IN AUTOIMMUNE AND CHRONIC INFLAMMATORY DISEASES.
Cervantes M., Penton Roll G.
Center for Genetic Engineering and Biotechnology. Havana, Cuba.
Organ specific or systemic autoimmune (AID) and chronic inflammatory diseases (CID), have been largely
associated to the lack or failure of immune system regulatory an tolerance induction mechanisms. Mostly as a
result of combined genetic, immune regulatory and environmental factors. The genetic predisposition conferred
by the HLA have been described but probably not enough to the self-development of AID which are complex
diseases that require the confluence of many genes and other factors. Tolerance to self is achieved through
multiple and diverse mechanisms. Heading these processes is suppression of immune responses by regulatory
T cells. Regulatory T cells control immune responses to self-antigens thereby preventing autoimmunity, and limit
responses to foreign antigens thereby minimizing T cell-mediated immunopathology. Natural products have
been, and continue to be, a major source of pharmacologically active substances from which drugs can be
developed. Natural products capable of modulating the immune system as well as its mechanisms of cytokine
production and activation e.g : c-Phycocyanin, Resveratrol, Royal jelly, Boswellic acid, Gum resin of Boswellia
serrata tree, N-acetylcysteine, Fish oil, Curcumin (diferuloylmethane), Bromelain, Vitamin-C and vitamin-E, are
known. Treg cell's essential role in the management of immune response to specific auto, allo and tumor
antigens has also been detailed. Balance between regulatory and effector arms of the immune system is a
necessity for immune homeostasis. The importance of direct manipulation of Treg cells using “disease modifying
therapies” from natural origin to attenuate hypersensitivity, cancer, infection, autoimmunity and alloimmunity and
their further related benefits will be discussed.
OC-34
PKC ACTIVATION BY PHORBOL ESTERS ISOLATED FROM JATROPHA CURCAS
Li N, Wilkins R, Rauf A, Sim C, Dimaki C*
School of Biology, Newcastle University, KGVI building, Newcastle upon Tyne, NE1 7RU, United Kingdom.
*E-mail: [email protected]
Phorbol esters are plant diterpenes based on the tetracyclic tigliane and mainly occur in the family
Euphorbiaceae. Phorbol esters show toxic effects in animals such as inflammation, cell proliferation and
differentiation and promote tumour formation. They are also activators of protein kinase C (PKC), by mimicking
the normal activator, diacylglycerol (DAG). The toxicity of the seeds and oil of Jatropha curcas (Euphorbiaceae)
a plant promoted for biodiesel and animal feed production is partly attributed to the presence of phorbol esters,
unique to this species. The bioactivity therefore of the phorbol esters needs to be understood. We report here
the fractionation of individual phorbol esters from the seed, oil and defatted seedcake of J. curcas and
determination of their PKC activity.
The phorbol esters were extracted from the plant material using methanol and their concentration was
determined using RP-HPLC with internal standard the phorbol myristate acetate (PMA), a synthetic analogue
with high PKC activity. The mixture of phorbol esters was fractionated by analytical HPLC to high purity as
determined by diode array detection. The isolated fractions were assayed for activity using TruLight TM PKC-α
assay kit (Merck Chemicals Ltd.) which is based on quenching of fluorescence by the phosphorylated peptide.
The activity of the J. curcas phorbol esters was evaluated along with a range of synthetic phorbol esters.
Phorbol esters profile differed among jatropha extracts of different origin (seed, oil and seedcake). Fractions of
J. curcas extracts at the concentrations run showed considerable activation of PKC in comparison to the control.
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OC-35
ROLE OF BIOACTIVE FLAVONOIDS AGAINST OXIDATIVE STRESS IN EXPERIMENTAL
REFLUX OESOPHAGITIS
Ch.V. Rao, M. Vijayakumar, S.K.Ojha and A.K.S. Rawat
Pharmacology Laboratory, National Botanical Research Institute, Lucknow – 226 001, Uttar Pradesh, India. Email: [email protected]
Introduction: The oesophageal reflux disease has gained clinical and surgical importance over the past decade
and it is a common disease that affects children and adults. Oxygen-derived free radicals and superoxide anions
are the main cause of free radical damage in reflux oesophagitis.The preventive effect of bioflavonoids rutin,
quercetin, and kaempferol, on experimental reflux oesophagitis in rats was investigated. Materials and
Methods: Rats separately received rutin quercetin, and kaempferol (100mg/kg x 3days; p.o x o.d), omeprazole
(30 mg/kg; i.p x o.d) and given last dose at 1 h prior to surgery. Oesophagitis was induced by ligating between
the fore stomach and glandular portion followed by pylorus ligation. Results: Rutin, quercetin and kaempferol
significantly inhibited the oesophagitis index to 1.12 ± 0.15 (P<0.001), 1.42 ± 0.12 (P<0.001) and 2.48 ± 0.12
(P<0.001) respectively, as compare to control group 3.2 ± 0.23. Further, acid and pepsin out put of gastric
contents were significantly decreased in treated groups. Rutin significantly inhibited the lipid peroxidation (from
0.68 ± 0.024 to 0.34 ± 0.04 nmoles of malonyldialdehyde (MDA) / mg protein) (P <0.001) and increased in levels
of catalase to 28.5 ± 1.6 units of catalase activity/ mg protein and superoxide dismutase (SOD) to 88.4 ± 7.5
units/ mg protein (P <0.001). Quercetin and kaempferol also showed significant inhibition in lipid peroxidation
(0.38 ± 0.02, and 0.44 ± 0.02) (P <0.01) and enhanced the activities of catalase (24.3 ± 2.6 and 21.5 ± 3.4) (P
<0.01) and SOD (85.3 ± 7.2 and 78.60 ± 6.5) activity. Rutin, quercetin and kaempferol treated group significantly
increased the glutathione level to 38.5 ± 2.42 (P <0.01), 35.4 ± 3.28 (P <0.01) and 30.5 ± 3.32 (P <0.05)
respectively. Rutin, Quercetin and kaempferol significantly attenuated the elevated level of collagen in
oesophageal tissue as of the omeprazole. Conclusion: The results suggest bioflavonoids could attenuate the
severity of reflux oesophagitis and prevent the oesophageal mucosal damage.
OC-36
INTERFERENCE OF SELECTED FLAVONOIDS WITH THE GROWTH OF FILAMENTOUS
FUNGUS R. nigricans
Lenasi H1, Kužnik M1, Abram V.2
1
Institute of Biochemistry, Medical Faculty, University of Ljubljana, Vrazov trg 1, SI-1000 Ljubljana, Slovenia,
[email protected]
2
Chair of Chemistry, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana,
Slovenia, [email protected]
A saprophytic fungus Rhizopus nigricans (R. nigricans) is a potential pathogen; it may cause some allergic
reactions in immunocompromised persons because it interferes with human respiratory tract. Many of generally
known fungicides are noxious to human health, therefore it is desirable to find some natural products with
inhibitory action on fungal growth, and possibly positive influence on individual healthiness. Natural plant
compounds, such as polyphenolics flavonoids, might meet these requirements. Flavonoids are produced by the
host plant during fungal infection and interfere with signaling involved in fungal growth and virulence.
R. nigricans can be easily grown in vitro. Our previous studies revealed that some steroids, especially
progesterone, were fungitoxic; in addition, progesterone activated in the fungus signaling pathway containing
cytosolic progesterone receptors. In the present report we investigated the interaction of R. nigricans with
flavonoids in comparison to fungitoxic progesterone. Fungal growth inhibition by selected compounds (100 µM)
was examined in agar, results expressed in percent of inhibition of hypheal radial spreading: biocanin a (100%
inhibition), progesterone (85%), flavone (82%), α-naphthoflavone (49%), chrysin-dimethylether (48%), 6methoxy-flavone (33%), 6-hydroxyflavone (26%), chrysin (20%), kaempferol-trimethylether (14%), genistein
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(4%). These results indicated the important role of the hydrophobicity of compounds in their fungitoxic effect. All
the above listed flavonoids were also tested as ligands of progesterone receptors in fungal cytosol. Results were
obtained by competitive analyses using (3H)progesterone and different excesses of nonlabeled flavonoids.
Competition curves and EC50 values revealed the binding of flavonoids to a putative allosteric binding sites on
the receptor. So far, we were not able to elucidate the biological role of flavonoid binding to progesterone
receptors in R. nigricans, thus further investigation is needed.
In conclusion, some natural flavonoids exert toxic effects on R. nigricans and may be used as antifungal
compounds. However, the mode of their action as inhibitors of fungal growth is not known yet.
OC-37
ANTIMICROBIAL ACTIVITY OF TAMARINDUS INDICA L. LEAVES EXTRACTS AND THEIR
CORRELATION WITH POLYPHENOL AND FLAVONOID COMPOSITION.
Escalona JC1, Urdaneta I1, Camacho M2, Rodríguez J1.
1
Pharmacy Department Universidad de Oriente, Ave Patricio Lumumba S/N CP 90500, Santiago de Cuba,
Cuba. E-mail [email protected]
2
Centre for Studies in Industrial Biotechnology. Universidad de Oriente, Santiago de Cuba.
Tamarindus indica L., or tamarind as is usually known, is a medicinal tree with ethnopharmacological uses in
microbial diseases. In the present work, the antimicrobial activity of four primary extracts from Tamarindus indica
L. leaves, and other five fractions obtained by successive liquid-liquid separation of the most active primary
extract, were evaluated against Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis, Escherichia
coli, Pseudomonas aeruginosa, Salmonellas typhimurium and Candida albicans, employing both disk diffusion
and the macrodilution tests. Totals phenols as tannic acid and flavonoids as quercetin were also calculated to all
extracts by calibration curve, computing the relationships between these chemicals variables and the
antimicrobial activity against each specific bacteria. A disclosure was the not appreciable relationship between
these variable, with the exception in Bacillus subtilis in which good correlation with total phenols were
recognized. Primary extracts showed acceptable levels of activities against Gram positive bacteria, larger than
for Gram negative, and inactivity against Candida albicans. Fluid extract at ethanol 70% exhibit a broad
spectrum and the high antimicrobial activity in comparison to the others primary extracts, having too the lofty
capacity for phenols and flavonoids extraction with 18.494 y 0.369 mg/mL. Aqueous extracts and in particular
the obtained from fresh leaves is the less active. The evaluation of the five fractions obtained from the fluid
extract in ethanol 70% show the impossibility to find activity for a single fraction while no one was active in lesser
doses than the primary fluid extract.
OC-38
ANALGESIC PROFILE OF MANGIFERA INDICA L. EXTRACT (VIMANG®) IN PATIENTS WITH
CHRONIC PAIN
Garrido B1*, Garrido G**, Delgado R1, Valverde S2, Ducangé D3, Duarte EM, Rabí MC, Bosh F, Porro J,
Hernández C4
1
Department of Clinical Pharmacology, National Center Coordinating of Clinical Trials, 200 St at 21 Ave, Playa,
Havana City, Cuba.
2
Ancient's Home Santovenia. Calzada del Cerro # 1424. Cerro, Havana City
3
Salvador Allende Hospital. Calzada del Cerro # 1551. Cerro, Havana City
4
Pain Clinic 10 de Octubre Hospital. Calzada de Díez de Octubre # 130 entre Alejandro Ramírez y Agua Dulce.
Cerro, Havana City, Cuba.
*Corresponding author: e-mail: [email protected]
It has been accepted that neuroinflammation, oxidative stress and glial activation play a roll in the central
sensitization underlying in the neuropathic and inflammatory chronic pain. Vimang is an aqueous extract of
Mangifera indica L. traditionally used in Cuba for its anti-inflammatory, analgesic, antioxidant and
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immunomodulatoy properties. In the present study, we determined the effects of Vimang's tablets and other
formulations in persons with chronic pain. The Complex Regional Pain Syndrome (CRPS) (n=15) and Pain
Associate with Zoster patients (n=12) received a daily dose of 1800 mg of extract (three coated Vimang tablets,
300 mg each, before meals, and cream 1,2 % as topical agent for 120 and 90 days respectively ). Compress
containing the disolution 2 % was utilized on skin lesions in 6 Herpes Zoster (HZ) patients for 30 days. In knee
osteoarthritis (OA) patients (n=10), 3 groups were design, group 1 n=3 received a daily dose of 1800 mg
(tablets), group 2 n=3 900 mg (tablets) and group 3 received a combination of tablets 900 mg and cream for 90
days. The change in average daily pain daily score through a Likert scale, the area and rate of dynamic
allodynia, rate of thermal allodynia, and burning spontaneous pain frequency were evaluated. The deep somatic
allodynia and joint's function in CRPS patients were evaluated too. The WOMAC Index (Western Ontario and
McMaster Universities) was utilized in knee OA and evaluated the presence of synovitis and effusion for
ultrasonography. From week 2 pain scores and sensory abnormalities showed a significant greater
improvement. The joint's function increased in CRPS patients. In acute Herpes Zoster patients, the analgesic
effect was observed from week 1 and none developed postherpetic neuralgia (PHN). In patients with persistent
pain less than 3 months post HZ and PHN, pain scores and sensory abnormalities showed a significant
improvement from week 4. The analgesic effect and improved joint's function were observed from week 2
associated with the decreased of the synovial effusion in knee OA patients. Booth effects, the increase and
decrease in synovial thickness (mm) were independent of the analgesia. The inhibition of the proliferation was
significant only in the group 2.
Our results suggest that Vimang's formulations might be appropriate to prevent and treat chronic pain.
1
This works was development the last year in the Department of Biomedical Research, Center of
Pharmaceutical Chemistry, actually, Center of Biomolecular Chemistry.
** Garrido G working in this moment at University of Chile.
OC-39
LIPID-LOWERING THERAPY COMPARISON WITH ATORVASTATINA AND POLICOSANOL ON
THE REDOX STATUS IN DYSLIPIDEMICS WITH PERIPHERAL VASCULAR DISEASES.
Fleitas Estévez AS*, Martínez G**, Seuc A*, Ameneiro S*, Fernández Montequin JI*, Delgado Roche L**,
Zapata J*, Morejon O*
*Instituto Nacional de Angiología y Cirugía Vascular (INACV). **Centro de Estudios para las Investigaciones y
Evaluaciones Biológicas. Instituto de Faramacia y Alimentos (IFAL), Universidad de La Habana. Laboratorios
López (Guatemala-Salvador).
It has been shown that a decrease in the concentrations of total cholesterol and LDL-col by diets and drugs
reduces the risk of peripheral vascular disease. Policosanol and atorvastatin have shown they are capable of
reducing both parameters in type II hypercholesterolemia. Some of the steps in the mechanism of action of
these drugs are differents and could show results sums, when we used in combination. They also show a
pleiotropic effect given by its general antioxidant action and antioxidant effect on the peroxidation. Objectives:
To compare the effect of treatment with the combination with Atorvastatin and Atorvastatin-Policosanol on
cholesterol concentrations of atherogenic lipoproteins, and the possible antioxidant action. Patients and
Methods: We studied 104 patients of both sexes in a range of ages between 40 and 60 years. It was taken as a
criterion for inclusion Col-LDL levels above 3.3 mmol / L. Patients were randomly distributed into two groups
(one treated with atorvastatin and the other with Atorvastatin + Policosanol). Measurements were made of
serum lipoproteins, and hemodynamic studies and oxidative stress before and after three months of treatment.
Results: Was found when comparing the groups among themselves, a decrease (p <0.05) in the values of T
Col, Col-LDL, TG, VLDL and RAC. Average values were lower (p <0.05) from Col-LDL in the group treated with
the combination of both drugs, as well as variations in the parameters which measure oxidative stress (GSH (p
<0.05) increased and MDA decreased). Conclusion: The combination of Policosanol and Atorvastatin in the
treatment of hypercholesterolemic patients offers better results than using each separately.
Keywords: Atorvastatin, Policosanol, hipocholesterlomic effect, oxidative stress.
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OC-40
EFFICACY OF MIGRAPRECOL IN THE PREVENTION OF MIGRAINE. RESULTS OF A FASE III
CLINICAL TRIAL.
Avila Y1, Reyes R2, Marrero MA1, Gil G1, Barrese Y1, Uranga R1, Olivera I3, Machado N2,3, Pando A4,
Cordero A4, Eirez M1, Enrique L3, Brito I1, Ballagas CM1, Ramos VG1, Barrera ML1, Pérez A3, Fundora Y2,
Lovaina NM4 and Clinical Sites Investigators Group.
1
National Centre Co-ordinator of Clinical Trials, 200 and 21, Atabey, Playa, Ciudad Habana, Cuba. Telephone:
271-8935, 271-7345, 271-7397, 271-8308 ext. 120-121.
E-mail: [email protected]; [email protected]; [email protected]; [email protected];
2
“La Pradera” International Health Centre. 230 and 15 avenue, Siboney, Playa, Ciudad Habana, Cuba.
3
“Hermanos Ameijeiras” Hospital. San Lázaro # 701, Centro Habana, Ciudad Habana, Cuba.
4
Neurology National Institute. 29th # 739, Vedado, Plaza de la Revolución, Ciudad Habana, Cuba.
Introduction: Migraine is one of the most frequent neurologic disorders and affects near 13% of the adult
population Objective: To evaluate the efficacy and safety of Migraprecol, phytofarm composed by a mixture of
natural active principles in the prophylaxis of migraine. Matherials and Methods: Double blind, placebo controlled
trial. Setting: Neurological outpatient clinic. Participants: One hundred forty eight patients aged, 18-65 years
with migraine with three to six episodes a month. Interventions: There where conformed two treatment groups
(74 patients each). Treatment: 12 weeks with Migraprecol (tablet 45 mg) or placebo tablet once daily. 12 weeks
follow period. Main outcome measures: Primary end points: frequency, intensity and duration of headache.
Secondary end points: quality of life and number of days taken as sick leave and acceptability of treatment.
Results: 88 involved patients that completed data; the frequency, intensity and duration of the crisis were
significantly reduced in Migraprecol group compared with placebo. Intention to treat analysis of data from
148 patients supported the differences in favour of Migraprecol for the primary end points. The Quality of life
during the treatment period and the following was better in the Migraprecol group a 97.7% of the patients treated
with Migraprecol where controlled Conclusion: Migraprecol has a clinical important prophylactic effect in
migraine. No adverse events were found.
OC-41
VIUSID, A NUTRITIONAL SUPPLEMENT, IN PATIENTS WITH CHRONIC HEPATITIS C AND
CIRRHOSIS. A RANDOMIZED AND DOUBLE BLIND-CONTROLLED STUDY.
Dr. Eduardo Vilar*, Yoan Sánchez*, Bienvenido Gra**, Enrique Arús*, Raimundo Llanio***, Luis
Calzadilla*, Ali Yasells***, María del Rosario Abreu****.
*Departamento de Hepatología, **Departamento de Patología, ***Departamento de Gastroenterología,
****Departamento de Bioestadística.
Nacional Institute of Gastroenterology, Havana City, Cuba.
Introduction: Recent studies have validated the feasibilities of IFN-based therapies for decompensated cirrhotic
patients but have provided no data on benefit of therapy in disease progression and survival rates. Aims: The
efficacy and safety of antioxidant therapy in preventing disease progression in patients with chronic hepatitis C
with compensated and decompensated cirrhosis is unknown. Methods: One hundred patients with chronic
hepatitis C who had clinically suspected or histologically confirmed cirrhosis were randomly assigned in a 1:1
ratio to receive 3 sachets of Viusid or placebo daily for a maximum of 96 weeks. The primary end point was the
survival rate and the secondary end points were time to disease progression, defined as clinical hepatic
decompensation (ascites, encephalopathy, bleeding gastroesophageal varices and spontaneous bacterial
peritonitis) or MELD score impairments of at least 4 points as compared with the pretreatment score, and
hepatocellular carcinoma. An independent data and safety monitoring board monitored the progress of the study
and performed interim analyses of the data. The study was terminated after a median duration of treatment of 64
weeks (ranges, 24-96) owing to a significant difference between each group of treatment and number of end
points reached. Results: Primary end point was reached by 92 percent (46 of 50) of patients receiving Viusid
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and 72 percent (36 of 50) of those receiving placebo (hazard ratio, 3 [95% CI. 1.09-9.2]; P=0.035). The
secondary end point was reached by 50 percent (25 of 50) of patients receiving placebo in comparison with 25
percent (9 of 50) of those receiving Viusid (hazard ratio for time to disease progression, 3 [95% CI. 1.4-6.6];
P=0.002). Hepatocellular carcinoma occurred in 5 patients assigned to placebo as compared with 1 patient
assigned to Viusid (hazard ratio, 3.9 [95% CI. 0.5-33]; P=0.17). Viusid tolerability was excellent and none severe
adverse event was reported. Conclusions: Continuous treatment with Viusid delays clinical progression in
patients with chronic hepatitis C and cirrhosis by significantly reducing the time to disease progression and
increasing the survival rates.
OC-42
CLINICAL STUDIES CONSIDERATIONS OF ALL BOTANICAL PRODUCTS.
Marrero Miragaya MA
National Centre of Clinical Trial, Calle 200 esquina 21, Reparto Atabey, Playa. Telephone: 2718308, 2718935.
[email protected]
Background Botanical products are finished, labeled products that contain vegetable matter as ingredients. A
botanical product may be a food (including a dietary supplement), a drug (including a biological drug), a medical
device (e.g., gutta-percha), or a cosmetic under the Act. Methods: In this review, the authors will summarize
research on many different clinical studies design in different phases. Results: The initial clinical trial for a
botanical product currently marketed under the Dietary Supplement Health and Education Act (DSHEA) will
ordinarily be a well-controlled study capable of demonstrating effectiveness. In general, initial clinical
investigations of nonmarketed botanical preparations should be similar to those of marketed products. Because
of the lack of current marketing experience, however, greater concerns could exist about toxicity. Expanded
studies of botanicals have the same purpose as expanded studies of synthetic drugs, including further
evaluation of dose-response for favorable and unfavorable effects and evaluation of long-term safety and
effectiveness, different populations, different stages/severity of disease, and drug-drug interactions.
Conclusion: The results show that the sponsor were to eventually identify the active constituents in the new
drug application, the active constituents might not be identified during the investigational new drug applications
(IND) stage.
OC-43
MAGIC BULLETS AND POISON ARROWS: THE ROLE OF NATURAL MEDICINES IN TREATING
NEUROCOGNITIVE DECLINE AND NEURODEGENERATION
Scholey, A
Natural medicines, Brain Sciences Institute, Swinburne University, PO Box 218 Hawthorn, Victoria, 3122
AUSTRALIA. [email protected]
Like other degenerative diseases, dementia can be considered to be product of a pathological cascade involving
progressively accelerating neurotoxic interactions between oxidative stress, inflammatory responses,
neurohormonal pathology and compromised cerebral metabolism. These lead to pathological endpoints which,
in the case of Alzheimer disease, include neurofibrillary tangle generation and -amyloid deposition, damage to
the cholinergic system. Thus neurodegeneration can best be viewed as a “multiple systems breakdown“. Given
the multifaceted nature of AD, it is perhaps unsurprising that monopharmacological treatments have had little
impact. It may be that systemic polypharmacology inherent in herbal medicines offers a more promising
approach to treating neurocognitive health. Herbal medicines often posses a complement of multiple
components with different pharmacological targets and some of these are extremely pertinent to processes
underlying neurocognitive decline. This talk will present specific empirical examples which make a case for the
development and use of herbal medicines in the treatment of neurodegenerative disease.
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OC-44
LINALOE ESSENTIAL OIL: ITS THERAPEUTIC REINCORPORATION
Lic. Eva Obregón Dominguez
Instituto Mexicano de Aromaterapia S.C.
Introduction: The Linaloe tree (Bursera linaloe, Burseraceae family) native from Mexico, growing in the area
known as Alto Balsas in Guerrero. Since the XVII century this essential oil has been obtained through
hydrodistillation. Materials and Methods: This Essential Oil was obtained from the fruits of the tree, keeping in
mind not to damage the species (ecologically), which was already deteriorated due to the over-exploitation. The
sample comes from the 2007 August and September harvest obtained from steam distillation. The chemical
analysis was obtained testing the CG gases chromatography with hexano. Results: The Essential Oil has the
therpens chemical characterization. The main components are Beta Mirceno, Limoneno, Geraniol, Linalilo and
acetate of linalilo. These 5 main components give the aromatic and therapeutic characters to the oil.
Bibliography: Hersch, P. and Glass, R. Linaloe: an aromatic challenge. Different dimentions of a Mexican
species, Bursera linaloe, Instituto Nacional de Antropología e Historia, Serie Científica. México D.F. 2006 ISBN:
968-03-0193-1.
OC-45
APHRODISIAC ACTIVITY OF CURCULIGO ORCHIODES GAERTN
Nagendra Singh Chauhan and V.K.Dixit
Department of Pharmaceutical Sciences, Dr. H.S. Gour University, Sagar (M.P.), 470003 India.
Curculigo orchiodes Gaertn, commonly referred to as ‘Kali Musli’, is widely recognized in Ayurvedic system of
Indian medicine as tonic and rejuvenator. The rhizomes are also considered aphrodisiac and sexual stimulant.
Ethyl acetate fraction of ethanolic extract of the rhizomes was studied for its effect on sexual behavior,
spermatogenesis, sperm count and hormone level. Fructose levels in seminal vesicles of albino rats were also
recorded. Two doses i.e. 5 and 10 mg/kg of ethyl acetate fraction of ethanol extract on administration in albino
rats showed pronounced anabolic and spermatogenic effect in animals of respective groups. The sperm count
and fructose levels in seminal vesicle were markedly increased. Improvement in sexual behavior of male rats
was characterized by increased mount and intromission frequency and reduced mount and intromission latency.
The extract had a dose dependent influence on sperm count and seminal fructose concentration which
increased significantly. The extract also increases testosterone level. These may be due to flavanoides present
in the extracts. These findings support the folk use of this plant as aphrodisiac.
Acknowledgements: Author N. S. Chauhan thankful to AICTE, New Delhi for providing fellowship.
References: 1. Chauhan, NS et al., (2007), Fitoterapia, 78,: 530-534.
2. Thakur, M., Dixit V.K., (2008), Sexuality and Disability, 26(1), 37-46.
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OC-46
THE PRECLINICAL AND SAFETY EVALUATION OF A TRADITIONAL MEDICINAL PRODUCT,
PHELA, A POTENTIAL IMMUNE MODULATOR.
Motlalepula G. Matsabisa1₣, Osman Ebrahim2, Ebrahim Yusoof2, Mohammed Dhansay1a, Jurgen Seier1b,
Monghezi Mdhluli1b Amaboo Dhai4 and Andrew Walubo3
1
Indigenous Knowledge Systems, Medical Research Council (MRC), 1aNutrition Intervention, Medical Research
Council (MRC), 1bPrimate Unit, Medical Research Council (MRC), 2Brenthurst Clinic, 3Department of
Pharmacology, University of the Free State, and 4Steve Biko Centre for Bioethics, University of the
Witwatersrand.
The use of traditional medicines in the management of HIV and AIDS is steadily increasing. There are several
reasons for this, including the fact that, there is no cure for HIV and AIDS, and there are no simple and
satisfactory programmes for managing this pandemic. On the other hand, because most traditional medicines
have been used for decades, they are often assumed to be safe and efficacious. Their concomitant use with
prescription drugs has not been well-studied, and in addition their efficacy has not been evaluated in controlled
clinical trials. Methods: Peer-reviewed and ethical methodologies have been developed to test the possible
toxicity of traditional medicines. The methodology, developed is a sub-chronic repeat dose toxicology study
using vervet monkeys (Cercopithecus aethiops), will be presented in respect of PHELA. The safety evaluation of
PHELA, using a parallel group, dose escalation and placebo controlled Phase I clinical trials will be presented. In
vitro and in vivo studies using cytochrome P-450 metabolizing enzymes have been developed to evaluate which
enzymes are involved in metabolizing traditional medicinal products. Results: The toxicological effects of
traditional medicines tested using sub-chronic non-human primate models have been conducted and will be
reported on. Most of the products have shown no toxicity at dose higher than would be consumed by humans
and also that some products have exhibited toxicological effects which are mild and reversible on withdrawal of
the product. The Phase I controlled clinical trial has shown that the toxicity exhibited in the vervet monkeys was
not reproduced in healthy human participants. The prescribed doses in the latter, however, were not at the same
levels. The herbal extract had no effect on the activity of CYP2D6, CYP3A4, CYP1A2, CYP2B6 and CYP2C9.
Conclusion: The results show that reliable controlled methodologies have been developed to evaluate the
preclinical toxicology, using non-human primate model, and the safety of traditional medicines. The study has
also shown how an in vitro system can be used to evaluate the effect of traditional medicines on P450
cytochrome enzymes and how traditional medicines are metabolised. Although in a Phase I study of PHELA, no
harmful side effects could be found, a preclinical study with vervets demonstrated toxicity. However, this was
due to the very high doses used in vervets. The results will further enable us to develop larger controlled clinical
methodologies to evaluate the efficacy of traditional medicines in multi centre studies.
OC-47
THE ROLE OF SCIENTIFIC EVIDENCE ON THE ATITUDES OF HERBAL MEDICINE
CONSUMERS OF DIADEMA (SÃO PAULO, SOUTHEASTERN BRAZIL)
Lanini J, Duarte-Almeida JM, Nappo S, Carlini EA
Departamento de Psicobiologia/ UNIFESP Rua Botucatu 862, 04023-062, São Paulo/SP, Brazil.
[email protected]
Medicinal plants (MP) have been used world-wide for treatment or prevention of health conditions, and due to
their natural origin, they have been considered harmless and are commonly used for self-medication. The aim of
the study was to evaluate the role of scientific evidence in the decision of making use of MP. Qualitative study
carried out with an intentional sample of users of MP. The participants were recruited by means of the chain
sampling method and they were inquired to answer a semi-structured interview, that was designed to provide
more reliable and complete information about the safety of MP as well as socio-demographic characteristics of
the sample. We also asked about the influence of scientific evidence versus TV information on the believes and
atitudes in MP of the volunteers. The participants included 13 women and 5 men (average age = 54,4). All the
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participants declared to believe that MP are completely harmless. The majority (n=11) of them reported that they
would continue their treatment besides a negative scientific report. Moreover, 16 volunteers declared that
information from TV programs were more significant for their decision than scientific reports. Users tend to rely
on personal types of evidence to make decisions regarding MP use. The same observations have also been
reported by other authors (Vanderheyden et al., 2004). Our study strengthens the call for more research and
educational efforts into this complex area.
Financial Support: AFIP/CNPq/FAPESP
CARTELES (P) / POSTERS (P)
JUEVES 4 de Junio / THURSDAY, June 4 - VIERNES, 5 de junio / FRIDAY, June 5
CARTELES FARMACOLOGIA PRECLINICA / POSTERS PRECLINICAL PHARMACOLOGY.
PPP-01
CYTOTOXIC ACTIVITIES OF PEROXIDASES AND
POLYPHENOLS AND OTHER NATURAL COMPOUNDS
HYPOHALOUS
ACIDS.
PROTECTION
BY
Ronca F.°*, Tagliazucchi D.^, Maltinti S.°, Gondres Amaro Y., Ronca G.°
°Department of Human and Environmental Science, via Roma 55, 56121 Pisa Italy
^ Department of Agricultural Science, via Amendola 2, 42100 Reggio Emilia, Italy
*[email protected]
Mammalian peroxidases (XPO) have a role in both disease prevention and some pathologies. The oxidants
(hipohalous acids, halamines, reactive oxygen species…) generated by myeloperoxidase and eosinophil
peroxidase defend the organism from bacteria, parasites and other invading pathogens. Peroxidases closely
related to or identical to lactoperoxidase have a role in oral hygen and airway defence. The initiation and
accumulation of oxidative damages mediated by XPO play a role in progression of atherosclerosis and
cardiovascular diseases, cystic fibrosis, Alzheimer disease. The protective effects of natural compounds such as
polyphenols present in foods and beverages against oxygen reactive species (ROS) has been extensively
investigated. Few data are available on their effects toward the cytotoxic activity of peroxidases and hypohalous
acids.
The cytolytic activity of XPO-H2O2-halide ion system and hypohalous acids on human erythrocytes has been
used to evaluate the protective effects of wines, vinegars, teas, polyphenols and hydrophilic extracts of some
vegetable and fruits.
After a lag of several minutes in the presence of XPO-H2O2-halide ion or hypohalous acids erythrocyte lysis is
observed. Wines, vinagres and teas increase the lag period and the time of 50% lysis. The protective effect
depends from the total polyphenols content, type of polyphenols and it is concentration dependent. The
protective effect is not simply related to their capacity to scavenge ROS. Hydrophilic extracts of artichoke, white
cabbage, melon and apricot are very effective in protection of erythrocytes from lysis. The protection efficacy is
not related to ascorbic acid content. The results obtained show that polyphenols and other natural compounds
present in foods and beverages protect not only from ROS damages but also from toxic effects of XPO due to
the formation of hypohalous acids.
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PPP-02
BIOLOGICAL ACTIVITY OF MELANOIDINS FROM TRADITIONAL BALSAMIC VINEGAR OF MODENA AND
REGGIO EMILIA
Tagliazucchi D., Verzelloni E., Gondres Amaro Y., Conte A.*
Department of Agricultural and Food Sciences, via Amendola 2, 42100 Reggio Emilia, Italy
*[email protected]
Scientific interest for high molecular weight melanoidins (HMWM) has increased because of their biological
activities and functional properties. Traditional balsamic vinegar (TBV) is produced by the alcoholic fermentation
and acetic biossidation of cooked and concentrated grape must and subjected to at least 12 year of ageing with
sequential transfer in 5 wooden casks of different volume. The final product is drawn from the smallest cask.
TBV is a dark, thick and aromatic product containing high sugar concentration, organic acids, polyphenols and
melanoidins. The aim of this work is to verify the formation of melanoidins and their contribution to the total
antioxidant activity of TBV during ageing process.
Samples came from two sets of barrels (A and B) composed of 5 wooden casks at different ageing. HMWM (>
10KDa) were separated from low molecular weight compounds. The TBV and HMWM samples were analyzed
for their reducing ability (ferric reducing/antioxidant power FRAP assay ) and radical scavenging activity (ABTS
assay). The amount of HMWM was determined by measuring the browning index at 420 nm and by weighing
after drying of the samples.
During TBV ageing there is an increase of the antioxidant activity and a gradual synthesis and accumulation of
HMWM. The increase in antioxidant activity is highly correlated to the increase in the amount of melanoidins
measured in the fractions (r= 0.954; P= 0.012 and r= 0.923; P= 0.026 for the set of barrels A in the ABTS and
FRAP assays, respectively, and r= 0.991; P= 0.001 and r= 0.990; P= 0.001 for the set of barrels B in the ABTS
and FRAP assays, respectively). We found that HMWM may contribute up to 50 % of the total antioxidant
activity of aged TBV and can play an important role in protecting the gastrointestinal tract from oxidative
damage.
PPP-03
INFLUENCE OF GRAPE VARIETY ON NEUROPROTECTIVE AND VASODILATORY EFFECT OF RED
WINES
Martín1 S., Gómez-Serranillos1 P., Ortega1 T., Palomino1 O., Carretero1 E., Prodanov2 M., Vacas2 V.,
Cabellos2 M., Estrella3 I., Hernández3 T.
1
Dpt. Farmacología, Universidad Complutense de Madrid, 28040 Madrid, Spain. 2Dpt. Food Chemistry, IMIDRA,
Ctra. A-2, km 38.200, 28800 Alcalá de Henares, Madrid, Spain. 3Instituto de Fermentaciones Industriales
(CSIC), C/ Juan de la Cierva, 3, 28006 Madrid, Spain. E-mail: [email protected]
Introduction: - Numerous studies have shown that moderate red wine consumption can evoke beneficial effects
to human health as cardioprotection and/or neuroprotection. This effect has been attributed, in part, to the
phenolic compounds present in wines, which shown qualitative and quantitative differences, depending on
factors such as grape variety and winemaking technique. The aim of this work was to study the vascular smooth
muscle relaxation and the neuroprotective effects of Spanish red wines and to relate them to their polyphenolic
composition.
Materials and Methods.-Monovarietal red wines were obtained from Vitis vinifera L. grapes: Merlot,
Tempranillo, Garnacha and Cabernet-Sauvignon, cultivated at one and same agricultural and climatic
conditions, at similar conditions of winemaking.
For Neuroprotection study, survival rate of human astrocytoma U-373 MG cell culture was assessed against
three toxins, FeSO4, H2O2 and FeSO4 + 1 H2O2 with respect to the studied wines. In the quantitative assessment
of cellular viability and proliferation, MTT reduction assay was used. Cellular damage was quantitatively
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evaluated by the amount of released lactate dehydrogenase (LDH), which activity was measured by the
reduction of NAD+ to NADH.
Vascular reactivity experiments were carried out on thoracic rats aorta rings (Sprage-Dawley). Vasodilatory
effects of different doses of wines were studied on arteries pre-contracted with norepinephrine 10-6 M. Isometric
contractions were recorded by a Grass model FTO3D force-displacement transducer/79E polygraph.
Phenolic compounds were identified and quantified by HPLC-DAD-MS.
Results and Conclusions.-The polyphenolics identified in wines were hydroxybenzoics, hydroxycinnamics,
flavanols, flavonols, anthocyanins and stilbenes
Every wine showed dose-dependent vasodilatory response and neuroprotective effect. Wine from Merlot variety
induced the maximum effect for both assays, this wine showing the major content of proanthocyanidins,
flavonols and resveratrol.
PPP-04
ESTUDIO DEL POTENCIAL CITOTÓXICO DEL GLICOLIPIDO TETRASACARIDO STANCINA
Ipomea stans, SOBRE LINEAS CELULARES
IV, DE
Elizalde, J.(1), León, I(2) y González-Christen, J(1)
(1) Facultad de Farmacia, (2) Facultad de Ciencias. Universidad Autónoma del Estado de Morelos. Av.
Universidad 1001, col Chamilpa. México. E-mail: [email protected]
INTRODUCCION: Ipomoea stans es una planta que se emplea en la medicina tradicional mexicana para tratar
enfermedades renales y biliares, entre otros usos. De esta planta se aisló un glicolípido tetrasacárido,
denominado Stancina IV, para el que se mostró un efecto citotóxico sobre las líneas KB, HCT-15, UISIO-SQC1 y OVCAR-5. En este trabajo nosotros proponemos continuar el estudio de este potencial, analizando nuevas
líneas celulares, particularmente de origen linfoide, y ampliando los estudios para determinar las
concentraciones letales medias (CL50).
MATERIALES Y METODOS: Se utilizaron las líneas celulares THP-1, Jurkat y MDCK de la compañía ATCC. El
efecto citotóxico del compuesto se determinó a las 24, 48 y 72Hrs de incubación mediante la técnica de MTT.
Los resultados se analizaron en un lector de placas marca Labsystem a 570nm. La CL50 se determinó mediante
el ajuste de la curva concentración vs viabilidad.
RESULTADOS: Nuestros resultados muestran que el compuesto indujo la muerte de las tres líneas celulares
analizadas, teniendo un efecto máximo a las 48 horas. Las CL50 fueron para MDCK de 12.3µg/ml, para THP-1
<4µg/ml y para Jurkat de 5.9µg/ml.
CONCLUSIONES: El glicolípido tetrasacárido Stancina IV de Ipomoaea stans es capaz de inducir la muerte de
un amplio espectro de células de diferente origen, a concentraciones letales medias de 12 a >4 g/ml, por lo
que de acuerdo a las guías del NCI ouede ser una molécula adecuada para estudiar como anticancerígeno.
Aun faltan realizar pruebas de toxicidad y mecanismos de acción.
PPP-05
CONTENIDO DE POLIFENOLES TOTALES Y CAPACIDAD ANTIOXIDANTE EN EXTRACTOS ACUOSOS Y
ETANÓLICOS DE HOJAS Y CORTEZA DE Ochroma pyramidale (Cav. ex. Lam)
Tello W1,2*, Guerrero T2, Sandoval M1
1
Universidad Nacional Agraria de la Selva, Centro de Investigación de Productos Naturales de la Amazonia,
Tingo María, Perú, Av. Universitaria s/n. Km 1.5,*[email protected]
2
Universidad Nacional Agraria de la Selva, Facultad de Recursos Naturales Renovables, Tingo María, Perú, Av.
Universitaria s/n. Km 1.5
Ochroma pyramidale (Cav. ex. Lam), es una planta medicinal de la Amazonia usada en el tratamiento de la tos
seca, diarrea, cólicos, reumatismo y dolores de las articulaciones, así como también para combatir infecciones
del pecho, bronquitis y gripe; los extractos de esta planta mostraron actividad en vacas para asistir la expulsión
de la placenta después de parto. Sin embargo, varios de estas acciones no han sido demostrados
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científicamente; por tal razón nuestro laboratorio durante los últimos 5 años ha venido investigando el valor
biológico de plantas de la Amazonia que poseen información etnobotánica y son utilizados en medicina
complementaria. El propósito del presente estudio fue evaluar el contenido de polifenoles totales, capacidad
antioxidante en los extractos acuosos y etanólicos de hojas y corteza. Los polifenoles totales fueron
determinados por método de Folin-Ciocalteu y la capacidad antioxidante fueron determinados por la inhibición
de 1,1 diphenil-2-picrilhidrazil (DPPH*) y peroxilos. El contenido de polifenoles totales fue 181,77±1,52 mg
CATE/g de muestra seca en extracto etanólico de corteza (P<0,01). El IC50 en la capacidad de secuestro de
radicales DPPH* y peroxilos fue 20,81±0,08 y 4,13±0,003 µg/ml respectivamente (P<0,01), en extracto etanólico
de corteza. En conclusión nuestros resultados indican que Ochroma pyramidale (Cav. ex. Lam), posee un alto
contenido de polifenoles totales, así como también buena capacidad antioxidante. Investigaciones adicionales
están siendo conducidas para determinar la mejor aplicación tecnológica de procesamiento para la elaboración
de nutracéuticos.
PPP-06
ANTIAGING ACTION OF MELATONIN
Prieto-Gómez B., Santibáñez-Olvera C., Moreno-López Y. L., Reyes-Prieto N. M. y Velázquez-Paniagua M.
Laboratorio de Neurofisiología Integrativa Depto. de Fisiología, Facultad de Medicina,
D.F.04510 ([email protected])
UNAM, México
Melatonin is considered the best endogenous antioxidant due to its capacity of crossing every cellular
membrane. Over the years, the concentration of melatonin is reduced in the organism and its antioxidant action
also falls. Diverse neurodegenerative diseases are associated with this decrease of melatonin and the increase
of free radicals. In a previous work, we showed that the chronic application of melatonin reduces the free
radicals in the CA1 and CA3 areas of the rat’s hippocampus. Furthermore, an increase of the expression of
MAP2 was described. Having demonstrated that there is a better cellular stability in the hippocampus with
melatonin, we hypothesized that these cells should also maintain their function. Two groups of Wistar rats were
employed for these analyses. Group 1 was treated with melatonin (M) and group two was the control (C) In
animals treated for 2, 4 and 8 months with melatonin, the unitary extracellular activity from CA1 hippocampal
area was recorded. The spontaneous activity in both M and C groups at 2 months of treatment showed regular
firing in the form of bursts, being greater the frequency in the rats dealt with melatonin. In the four months
treated rats, the frequency of neuronal firing was reduced in both groups compared with the youngest rats, but
the difference between C and M was conserved. After 8 months, the activity of melatonin rats was very similar
to the activity of the 2 months old control rats. The results indicate that, even though the animals are not “old”,
both groups show changes in the cell firing rate as time goes by and treating them with melatonin “preserves” a
greater frequency of firing.
PPP-07
MELATONIN REGULATES FERTILITY AND SEXUAL BEHAVIOR OF MALE RATS
1
Velázquez-Paniagua M., 2Parra-Gámez L., 1Gutiérrez-Ruiz J., 2García-Hidalgo A., 1Balderas-López
Eduardo J., and 1Prieto-Gómez B.
1
Laboratorio de Neurofisiología Integrativa Departamento de Fisiología, 2Laboratorio de Neuroanatomía
Funcional Departamento de Anatomía, Facultad de Medicina UNAM, México D.F. 04510
([email protected])
A decrease of testosterone in rodents and humans treated with melatonin was demonstrated. In previous
experiments, we showed that melatonin reduces the production of spermatozoa and modifies the
citoarchitecture of the seminiferous tubules. In this work, we determined the effects of the chronic application of
melatonin on fertility and sexual behavior of male Wistar rats and the reversion of these effects. Two months old
rats were utilized. They were divided in 2 groups: melatonin (M) with an i.p. daily injection of 1mg/kg of body
weight for 3 months; and control (C), with vehicle application. Phase 1, when the pharmacological treatment on
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both groups was concluded, we tested fertility and sexual behavior and analyzed the number of spermatozoa.
For the fertility analysis, the rats were left to mate and the number of pregnant females was evaluated. For the
sexual behavior test, the male rats were left to mate with ovariectomized and hormonally stimulated females. We
determined the number of copulatory rats, the accumulated number of copulatory series, the latency in
ejaculation and we also counted the spermatozoa. Phase 2, we kept both groups (this time without treatment)
for another two months and evaluated them with the previously described analysis. In the first phase, the M
group had a significant decrease in the number of spermatozoa and in fertility, and an increased sexual behavior
compared to the C group. On the contrary, in the second phase, both the number of spermatozoa and fertility
increased and the sexual behavior was reduced.
PPP-08
METABOLIC STUDIES ON THE ANTISPERMATOGENIC EFFECT OF CARICA PAPAYA LINN. SEED
EXTRACTION
Dr C. CHANGAMMA
Assistant Professor, Department of Zoology, S.V.University PG centre KAVALI – 524 201 (A.P.) INDIA. E mail:
[email protected]
In recent years more emphasis is being laid on male rather than female antifertility agents. A number of
synthetic compounds have been reported to arrest spermatogenesis or induced functional sterility, but were
unsuitable for human use due to toxic manifestations (Waites 1993). Now a days more emphasis is being laid
on plant materials which are ecofriendly in approach and interfere with the natural patterns of reproduction
(Dixit1992). It was therefore of interest to investigate the effect of biologically active botanical ecofriendly plants
such as carica papaya seed extract (Udoh & Kehinde 1999). In the present study healthy adult male Wistar
strain albino rats of 90 days age group weighing 120±10g have been selected for experimentation. The
experimental rats were administered with 5mg/kg body wt. /day of alcoholic extraction of papaya seed for 7days
intramuscularly. The extract was prepared according to WHO protocol CG-04 for the preparation of an alcoholic
extract (WHO 1983). After 24hrs from the last dosing the animals were autopsized and the testes were
isolated, chilled immediately and used for biochemical analysis. The glycogen metabolism of the testis was
evaluated. The decreased glucose and glycogen content was noted through accelerated glycogenolysis. This
might reveal its possible mobilization towards either hexose monophosphate or diphosphate pathway. The
concentration of the enzymes aldolase and Phosphorylases were elevated slightly. The elevated FDP-aldolase
suggest the stepped-up operation of hexose diphosphate pathway. The higher content of pyruvic acid showed
higher rate of glycolysis. The decreased content of lactic acid in spite of elevated glycolysis suggests the
possible stepped up mobilization of lactic acid into citric acid cycle. Since carbohydrate reserves were
decreased in the treated testes and the more mature germinal cell population depends on testicular
carbohydrate reserves, impaired germ cell structure and function can be suggested. The degenerative change
of germ cells like spermatocytes, spermatids and spermatozoa (Lohia et al 1999&Lohiya et al 2005) supports
such a possibility. The decrease in ATPase activity could be attributed to androgen dependent parameters
(Mohammad Hossein Dehghan et al 2005). The depleted energy reserves may be responsible for the impaired
spermatogenesis in papaya seed extraction treated rat testes.
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PPP-09
PROTECTIVE EFECT OF Bidens pilosa L. EXTRACT IN HEPATOTOXICITY INDUCED BY PARACETAMOL.
Rayza Méndez Triana MSc, María BoffIill Cárdenas PhD, Emilio E. Monteagudo Jiménez MSc, Maykel
Pérez Machín MSc1, Omaida López Bernal MD.
Medical College of Villa Clara. Clinical Trials Center. Acueduct Rd. and Circunvalation. Santa Clara, Villa Clara,
Cuba. E-mail: [email protected]
Introduction: Bidens pilosa L, known as romerillo blanco, is widely used by the cuban population. It is chemically
composed by flavonoids (quercetin) with proved antioxidant proprieties. Objective: A preclinical study was
carried out to evaluate the hepatoprotective effect of Bidens pilosa L in the toxicity induced by paracetamol.
Materials and Methods: Male adults MNRI mice were used to evaluate the effect of oral administration of threelevel dose (150, 300 y 600 mg/Kg weight) of Bidens pilosa L extract, 30 minutes before and 2 hours after the
hepatotoxicity induced by paracetamol (600 mg/Kg, oral, unique dose) establishing as markers the evaluation of:
clinical signals, enzymatic activity of alkaline fosfatase, alanine aminotransferase (ALT) and aspartate
aminotransferase (AST); and histological changes produced by hepatocites. Results: Abnormalities in the
behavior of animal, were not evidenced during the trials. The analysis of plasmatic concentrations of the tree
enzymes in the groups treated with the plant showed high significant statistic in respect to control group treated
with paracetamol. Values within the reference values range for the specimen were obtained.
Anatomopathological results confirm the cellular protection, either macroscopic or microscopically, in the livers of
the animals treated with the plant. Conclusion: To sum up, the administration of Bidens pilosa L extract showed
a protective effect on the liver against the toxicity of paracetamol, with no relation between dose and
pharmacologic action.
PPP-10
PRECLINICAL EVALUATION OF THE ANTI-INFLAMMATORY ACTIVITY OF WHITE BIDENS (ROMERILLO).
Pajarín F L, Álvarez G RM.
Instituto Superior de Ciencias Médicas Santiago de Cuba. Facultad Medicina I
E mail: [email protected]
Introduction: The use of the medicinal plants goes back to the oldest origins of the humanity. The past century
arose an alive interest by the study and consumption from the medicinal plants which opened a wide field to
them to application. Well-known white Bidens in our country like “romerillo” has been used in the treatment of
diverse anti-inflammatory affections,and it has inquired into its properties that motivated the development of this
investigation. Materials and Methods: An experimental study with the objective was made to determine the
effect anti-inflammatory. In these experiment were used: 30 Sprague Dawley male rats, 30 Wistar male rats and
30 Swiss mice. The determination of the anti-inflammatory activity using different pharmacological procedures
were used: the technique of granulomas induced by cotton discs, edema to plant by auricular carragenin and
edema induced by oil of croton according standardized protocols. The extract was used by oral way in three
different doses and its effect was compared with alcohol 50 % (vehicle of the extract), indometacin and
bicarbonate to 4 %. Results: The extract was administered to white Bidens in dose of 200mg/kg showing antiinflammatory action comparable with the obtained one with indometacin, using the technique of granulomas
induced by cotton discs. This extract is as effective as indometacin in the protection of plant edema induced by
carragenin. The extract (200 mg/kg) also inhibits the inflammatory response caused by the croton oil
administration. Conclusions: Preclinical evaluation of white bidens (Romerillo) showed anti-inflammatory
activity using different models of inflammation. The anti-inflammatory activity of the extract result similar to antiinflammatory drugs indometacin. These results permit to consider the employment of extracts of this plant as
phytopharmaceuticals products with anti-inflammatory activity, which could substitute anti-inflammatory drugs as
indometacin (or others AINEs), characterized these for their high gastrointestinal toxicity.
Key words: anti-inflammatory , Bidens (Romerillo)
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PPP-11
ANTI-INFLAMMATORY, ANTIOXIDANT AND ANALGESIC EVALUATION ON Murraya paniculata.
Casado C., Gutierrez Y., Garcia G., López R., Miranda M.
Pharmacy and Foods Institute. University of Havana. 23 Ave. # 21425 b/ 214 and 222. La Coronela. La Lisa.
Havana. Cuba. E-mail: [email protected]
Safety on the external use of Murraya paniculata or pain and inflammation relief in arthritis, arthrosis and other
rheumatic diseases has been settled by means of a study of Dermal Acute Toxicity, using the methodology
described in EODC # 402 Guides. This medicinal plant shows a wide spectrum of biological activities due to an
important number of biologically active components reported around the world.
In this sense, the anti-inflammatory effect of Murraya paniculata was evaluated, using the Induced Edema
Rehearsal, on 15 albino mice (OF1 - males) in 3 work groups: Negative Control, Positive Control (Bencidamina)
and Treated Group (Murraya paniculata). The antioxidant and analgesic potential were also determined by
means of FRAP (Ferric Reduction Antioxidant Power) and Hot Plate methodologies, respectively. Additionally, a
non probabilistic sample (60 patients) was interviewed, in order to evaluate the acceptance of the
phytopharmaceutical product produced from Murraya paniculata and the level of trust toward its attributed
therapeutic activity, as well as the security of its use.
The inflammation percentage produced in the ear was lower, compared to the value reported for the
inflammation produced with Croton Oil. The inflammation inhibition was similar for Bencidamina and Murraya
paniculata. There were found: a reduction power above 200µΜ, around a 90% of effectiveness of the treatment
with the phytopharmaceutical product, and no evidence of adverse reactions in patients.
The obtained results let us conclude that this phytopharmaceutical posses high potential for extensive use in
traditional medicine, and also for more phytochemical studies.
PPP-12
ANTI-INFLAMMATORY ACTIVITY OF TWO FLAVONOLIC EXTRACTS OF THE PETIVERIA ALLIACEA L. IN
RATS.
Gross Fernández MC.
Medical University of Santiago de Cuba. E-mail: mferná[email protected]
Introduction:The vegetable specie Petiveria alliacea L. (anamú), is one of the medicinal plants approved by
the Ministry of Public Health of Cuba for its integral study; it has been used for various generations for different
therapeutic uses; among these are the anti-inflammatory action. Materials and Methods: The Critical Route of
Investigation in Medicinal Plants with the elaboration of a herbalist medication for the treatment of Psoriasis;
however, the active substances for the anti-inflammatory activity are unknown; therefore assuming that
flavonoids are responsible for this action, without having carried out any scientific validation. Due to this, this
study is a pharmacological preclinical evaluation I of two flavonolic extracts of this plant to prove antiinflammatory action. These two soft extracts: one of the leaf and another of the root, elaborated according to the
procedure described in the Ramal Norm of Public Health 311 and using as menses, hydroalcoholic solutions at
30 and 50%, respectively; according to farmacognostic studies carried out. These extracts underwent liquid –
liquid fractioning, using solvents in ascending order of polarity: n-hexane, chloroform, ethyl acetate and nbutanol in a consecutive form, for the obtention of a flavonolic extract of the leaf and another of the root; both
from the n-butanol fraction and with a concentration of 10 mg/mL. With these two samples, the preclinic
evaluation was carried out using an internationally accepted biological valuation model for the search of new
non- steroidal anti-inflammatory drugs: "Subplantar Edema for Carragenin" in rats Sprague Dowley of the
masculine sex. The doses tested were: 100, 150 and 180 mg/kg of corporal weight for both extracts, taking into
account bibliographical records; a suspension of indomethacine 10 mg/Kg of weight as the positive control and
hydroalcoholic mixtures at 30 and 50% as negative controls. The administration route was intraperitoneal.
Results: The results demonstrated that both extracts at the three tested doses possess activity and antiinflammatory efficacy; therefore confirming that the flavonoids present in the soft extract of the leaf and the root
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are responsible one for the anti-inflammatory action attributed to this plant; although the action of other
metabolites cannot be discarded. Conclusions: It is also verified that the activity and efficacy increase as the
dose increases; without the observation of external signs of toxicity.
Key words: anamú, pharmacological preclinic, Petiveria alliacea L., anti-inflammatory preclinic, flavonolics
extracts.
PPP-13
ANTI-INFLAMMATORY AND CYTOTOXIC ACTION OF THE THYME (THYMUS VULGARIS).
Ferrándiz D1., Iglesias E1., Valls AR2., Pérez PL1.
University of Camagüey1. Circunvalación Norte Km. 51/2. [email protected]
Superior Institute of Medical Sciences of Camagüey2. Carretera Central Oeste s/n.
One of the fields that awake bigger interest in the evaluation pharmacognostic is the study of vegetable species
with anti-inflammatory properties. The flavonoids presence confers to some plants anti-inflammatory and antioxidant activity, among others. Presently work intended to evaluate “in vivo” the extracted flavonoid of the
Thymus vulgaris (thyme), starting from experimental models of inflammation, as well as to prove its cytotoxic
activity. The anti-inflammatory study was carried out by means of the inflammation method in mouse-ear,
caused by the administration of 13-acetate of 12-or-tetradecanonoiforbol (TPA) that acts as irritating agent to a
dose of 2 µg/ear. The mouse separated in group control, group study treaties with the flavonoid and a group of
reference treaty with dexametasone. The typical deviation is determined and the error type gives each one of
the obtained stockings, as well as the statistical significance of the differences using the test of Dunnett (P
<0,05). For the rehearsal of the cytotoxicity the method was used in vitro of the tetrazolio 3-(4,5-dimetiltiazol-2il)-2,5-difenil-bromide (MTT) that is based in the conversion of the MTT from yellow colour to its formazan of dark
blue colour. The viability of the cells is calculated by means of the expression: % viability = Ām/Āc x 100. He was
proven that the rehearsed compound reduces the inflammation induced by the TPA in 61%. According to the
results obtained in the cytotoxicity rehearsal, the compound doesn't present this activity in front of peritoneal
neutrophils to the concentration of 10 µM. Therefore, it could demonstrate himself the anti-inflammatory
effectiveness of the flavonoid and their null cytotoxic activity.
PPP-14
EVALUATION IN VIVO OF A FLAVONOID OF PIPER AURITUM IN A MODEL OF RETARDED
HYPERSENSITIVITY
Pérez PL, Ferrándiz D, Iglesias E, Valls AR.
Universidad de Camagüey. Circunvalación Norte Km 51/2 Email: [email protected]
Plant world brings at principles, with act through out different mechanisms or to different levels of the
inflammatory process, with the resulting additive or stimulating effect of their therapeutic propieties and scarce
tonicity. Flavonoids are phenolic compounds derived from 2-fenilcroman, that has been reconized since a long
time for having anti-inflammatory activity. Therefore, we focused on the evaluation of reactions with retarded
hypersensitiveness on an experimental model of the flavonoid taken from the Piper auritum. Swiss, female,
albino rats of 3 months old and about 25-30g weight werw used. The retarded hypersensitiveness reaction is
induced by external use, with a dosage of 2,4 dinitro-1-flourobencen. Two different procedures are followed.
One pre-treatment, were the products are given before the inflammatory reaction triggering, and another posttreatment, where the products are given after the reaction triggering. For the first procedure, the products are
applied at a term of 24, 48 and 72 hours, while for the second the same application is done within a period of 2,
24, 48 and 72 hrs. In both test ears thickness is measured with a micrometer at 24 and 72 hrs after the
inflammatory process. Hypersensitiveness reaction demostred a reduced inhibition percentage in 24 hrs for the
studied product and the regarded drug (dexametasona). It was proved that in 72 hrs the flavonoid had a 73
percent inhibition for the reaction which is considered efficient while comparing it to 83 percent obtained with the
dexamentasona.
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PPP-15
PROTECTIVE EFFECT OF MANGIFERA INDICA L. EXTRACT ON DEXTRAN SULFATE SODIUM- INDUCED
COLITIS IN RATS.
Márquez L1,2, Garrido G1, Gómez B2, Garate I2, Muñoz JL2, Menchén L3, Lesa Cerro JC2
Laboratorio de Farmacología, Centro de Química Farmacéutica, Playa, Ciudad de La Habana.
2
Departamento de Farmacología, Facultad de Medicina, Universidad Complutense de Madrid, España
3
Departamento de Gastroenterología, Hospital “Gregorio Marañón”, Madrid, España.
1
We investigated the effect of aqueous stem bark extract from Mangifera indica L. (MIE) on colitis in 4 % dextran
sulfate sodium-induced rats which were supplemented orally for 7 days. MIE (150 mg/Kg) was administrated in
two different treatment ways: one group was co-treated with MIE, rectal via during 7 days at the same time of
DSS administration (group B), the other group was co-treated with MIE, rectal via like group B, but moreover
received MIE (150 mg/Kg) orally during 14 days once a day prior DSS exposition (group C). General
observations of clinical signs were performed. Anti-inflammatory activity of MIE was assessed by neutrophil
infiltration and colonic lipid peroxidation, and determined by myeloperoxidase (MPO) activity and levels of
thiobarbituric acid reactive substances (TBARs), respectively in DSS system. Reduced glutathione (GSH) levels,
expression of inflammation related mediators (iNOS, COX-2, TNF-α and TNF receptors 1 and 2), IL-6 and TNFα serum levels were also measured. The results of experiment demonstrated that MIE have anti-inflammatory
properties by improvement of clinical signs and inhibition of MPO activity. In addition, administration of MIE 14
days previously to DSS exposition resulted in a decrease of GSH and TBARs levels, reduction of iNOS, COX-2,
TNF-α and TNF R-2 expression and depletion of IL-6 and TNF-α serum levels. In conclusion, MIE have antiinflammatory activity and the prior administration become it in a more effective agent. Therefore, we suggest this
product for the prevention and treatment of ulcerative colitis.
PPP-16
EFFECT OF POLICOSANOL FROM SUGAR CANE ON INFLAMMATORY AND WOUND HEALING IN
EXPERIMENTAL MODELS.
Boffill M1, Monteagudo E1, Martínez Y1, Villanueva G2, Quesada D1.
Experimental Toxicology Unit, Medical College of Villa Clara. Circumvallation Rd. and Aqueducts Rd.. Santa
Clara, Villa Clara, Cuba.
2
Faculty of Chemical and Pharmacy. Central University of Las Villas. Camajuani Rd, km 10. Santa Clara, Villa
Clara, Cuba. E-mail: [email protected]
1
Introduction: Policosanols are a mixture of high molecular weight alcohols, produced in the Chemical and
Pharmacy Faculty of Central University of Las Villas and other institutions. Those policosanols are attributed
anti-inflammatory and wound healing properties which are not experimentally evaluated yet, so the objective of
our work is to evaluate those properties in biological models. Material and Methods: wound healing effect was
evaluated using Balb/C mice administered with two preparations one of policosanol 1% in hydrophylic cream
and another one of policosanol 0,5% and 1% in sunflower oil. The mice were made a 2 cm diameter wound in
the escapular region. Anti-infammatory effects were evaluated in SD rats who were made plantar oedema by
using carragenin (acute inflammation) and subcutaneous granuloma (chronic inflammation) by cotton discs and
administered with policosanol in sunflower oil at doses of 200, 400 and 800 mg/kg BW. The effect on ear
oedema was done in Balb/C mice using PMA as inflammatory agent. Results: there is only significative antiinflammatory effect in the model of plantar oedema at the dose of 800 mg/kg BW and this preparation has no
effect on chronic inflammation so its action is very limited. There was a significative decrease of ear oedema
when policosanols in acetone were topically applied. Conclusions: This policosanols mixture has a discrete
wound healing action and its anti-inflammatory effect is very limited.
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PPP-17
EVALUATION OF THE DIURETIC ACTIVITY OF SIX PLANTS USED BY THE CUBAN POPULATION.
Pérez M1, Tamayo M2, Boffill M2, Lorenzo G3, Jiménez E2, Betancourt E2, Iglesias N2, Verdecía B2, Díaz L2.
Vice-Rectored research. University of Medical Sciences Dr. “Serafín Ruiz de Zarate Ruiz”. Aqueduct Road and
Circumvallation. Santa Clara, Villa Clara. CUBA.
2
Experimental Toxicology Unit (UTEX from its spanish equivalent). Dr. “Serafín Ruiz de Zárate Ruiz” University
of Medical Sciences. Aqueduct Road and Circumvallation. Santa Clara, Villa Clara. CUBA . Email:
[email protected]
3
ENCOMED (from its spanish equivalent). Santa Clara, Villa Clara. CUBA
1
Introduction: In Cuba exists about 179 medicinal plants which are known because of their diuretic properties
according to their traditional use, although in just some cases their effects have been proved experimentally.
Objective: To value the diuretic activity attributed to six medicinal plants used by the Cuban population: Ocimum
basilicum, Parthenium hysterophorus L, Justicia pectorales, Oreganum bulgharis L, Allium cepa and Citrus
aurantium L. Materials and Methods: There were prepared hydroalcoholic extracts from the dry drug of the first
four plants mentioned previously, and in the case of the Allium cepa and Citrus aurantium L from their natural
juice. There were distributed at random eight Sprague-Dawley male rats in each of the eight homogeneous
groups formed: group 1, received 20 mg/kg of furosemide (reference drug); group 2, received NaCl (0.9%), and
groups from 3 to 8 received doses of 400 mg/kg, using in all of the cases an 18G intragastric cannula. All the
treatments were administrated at a volume of 40 ml/kg. Rats were put in metabolic cages, where it was
measured the urinary excretion during the ½, 1, 2, 3, 4, 6 and 24 hours. Results: There was observed that all
treated groups increase their urine volume in relation with the negative control group. Urinary excretion, action
and diuretic activity were superior in the experimental group Allium cepa, similar to the action of the reference
diuretic. Conclusion: This research allowed us to confirm experimentally the popular use of six plants used by
the Cuban population.
PPP-18
IN VITRO EFFECT OF TROFIN®, AN HYDROLYZED OF PROTEINS AND HEME IRON MIXTURE ON IONIC
IRON REDOX STATUS
García Y a, Martínez Da, González Ra, Pardo-Andreu GLb
National Center of Biological Products (BIOCEN) Beltrán Mainroad, 1 ½ Km, Bejucal, Havana. Cuba. E-mail:
[email protected]
b
Centro de Estudios para las Investigaciones y Evaluaciones Biológicas. Instituto de Farmacia y Alimentos
.Universidad de La Habana. Ave. 23 # 21425 e/ 214 y 222, La Coronela, La Lisa. CP 13600, Ciudad Habana,
Cuba.
a
Iron salts as source of ionic iron are broadly used for the prevention and treatment of iron deficiencies, but they
cause adverse reactions in 25% of the patients. There are two different receptors in the duodenum for the
absorption of the ionic and the heme form of iron and that ferrous state is necessary for ionic iron absorption.
Therefore, a formulation containing the two type of iron at the same time could favor total iron absorption if it is
capable to preserve the ionic iron in its reduced state. Trofin® is an antianemic natural formulation containing
blood caw as heme iron source and proteins. The addition of a ferrous salt to the Trofin formulation may
increase its efficacy. Thus, the aim of this work was to evaluate the influence of Trofin® on ionic iron redox
status in order to propose a rationale for the inclusion of ionic iron into the Trofin formulation. It were prepared
four different combinations of Trofin® +Fe+2 in a w/w relation of: 500:1, 2 500: 1, 5000:1 and 10 000:1, each
containing 20 µM Fe+2 from Ferrous Sulphate salt in 50 mM HEPES buffer pH=6,5. We also studied the Trofin®
effect on Ferric iron and the influence of pH. The remaining Fe+2 concentration was spectrophotometrically
determined at 0, 5, 10, 15, 20, 25 and 30 minutes of Trofin®-iron incubation using 1 mM 1,10 Phenantroline at
510 nm. The oxidative damage of different Trofin® +Fe+2 mixture on brain homogenate of weaned rat was also
studied. Trofin® dose dependently caused a diminution of Fe2+ concentration, an effect that was prevented by
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Ascorbic acid. Trofin® also showed a slight reducing ability on ferric iron. The increased in Trofin® concentration
significantly decreased the lipid peroxidation in brain homogenate exposed to ferrous iron. These results
suggest that the development of new antianemic formulation containing Trofin®+ Fe+2 should be observed
cautiously and should comprise amounts of Trofin® below the proportion 500:1 w/w in relation to iron and should
contain ascorbic acid. The results also suggest for the first time an antioxidant effect for the Trofin formulation.
PPP-19
EFFECTS OF NATURAL ZEOLITE MODIFIED WITH ZINC (ZZ) ON STREPTOZOTOCIN-INDUCED DIABETES
MODEL IN RATS.
Torres A, 1 León O, 2 Casanova M, 2 Rodríguez G 3.
Instituto de Farmacia y Alimentos, Universidad de la Habana. San Lázaro y L, Ciudad Habana, Cuba. Email:
[email protected]
2
Centro de Estudios para las Investigaciones y Evaluaciones Biológicas. Instituto de Farmacia y Alimentos
.Universidad de La Habana, Cuba
3
Instituto de Ciencia y Tecnología de Materiales y Reactivos. Universidad de la Habana. San Lázaro y L,
Ciudad Habana
1
Diabetes is a serious problem of health at world for its high prevalence and its complications. Its development
and complications on various organs have been attributed to increased oxidative stress. Recently several
studies have been reported a relationship between the zinc functions and the oxidative stress associated to
diabetes. This has been associated among other factors to changes in the zinc metabolism that modify the
insulin answer and metallothionein antioxidative properties, which is very rich in cysteine and is an excellent
scavenger of reactive oxidative species. The homeostasis of Zinc is proposed as a key factor in the onset and/or
progression of the DM, observed by numerous examples in animal models and in clinical studies. In Cuba have
been designed and developed several modified natural zeolites. One of them is the e zeolitic natural product
activated with zinc ions named ZZ. ZZ performs as a control releaser of zinc. Taking into account we studied ZZ
antihyperglyceamia effect and its effects on oxidative stress markers levels.Five groups of rats were classified as
follow: (1) positive control using as a diabetes inductor, (2) non-diabetic control treated with vehicle solution (3)
ZZ group receiving 21 treatments (500 mg/kg), (4) ZZ group receiving 21 treatments (1000 mg/kg), (5) ZZ group
receiving 21 treatments (1500 mg/kg). The ZZ treatment improved glycemic control and prevented oxidative
stress. The results of this study show that repeated administration of ZZ might play a role in the control of
diabetes and its complications.
PPP-20
POLYPHENOL COMPOUNDS & PHARMACOLOGICAL REDOX MECHANISMS INVOLVED IN WOUND
HEALING. RHIZOPHORA MANGLE CASE STUDY.
Janet Sánchez1, Roberto Faure1, Gregorio Martínez2, Octavio Fernández1, Ernesto Vega1 and Evangelina
Marrero1.
1
Dep. Chemistry, Pharmacology and Toxicology, National Centre for Animal and Plant Health, CENSA,
Aptdo.#10, San José de Las Lajas, La Habana, Cuba. 2 Pharmacy and Food Sciences Institute, University of
Havana, Cuba. E-mail: [email protected]
Polyphenols are a large number of family compounds which are well represented in many plants all over the
world. Their pharmacological properties of many of them have been studied and it is well recognized the antiinflammatory, antioxidant, antimicrobial, among other biological activities. In particular the wound healing
process starts with an inflammatory phase which is accomplished of reactive oxygen species (ROS)
increasement; so to study the effect of polyphenolic compound on the REDOX mechanism involved in this
process was the goal of the present research. Rhizophora mangle (L.) bark aqueous extract and its major
component, high molecular weight polyphenols fraction, were evaluated for antioxidant and wound healing
properties on a rat (SD) model of aseptic wounds. The wound area (mm2), used as a parameter of wound
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healing was measured with Imagen Digital Processing System MADIP 3.0 Lab Lapdis, at 7 days after he
wounds were made. Different markers representing REDOX balance during the wound healing process were
also measured in the new formed tissue at 0, 3, 7 and 14 days comparing the evolution in treated and non
treated wounds: Dismutase Superoxide (SOD), Catalase (CAT), reduced Glutahione (GSH) and
malonildialdehyde (MDA). Results showed that the wound area was reduced in that treated with both the extract
and R. mangle fraction comparing with control groups (p< 0.05). Also the activity of antioxidants enzymes SOD
and CAT, and GSH concentrations were increased in presence of the extract, its fraction and positive control
(Ascorbic acid) compared to non treated control group (p< 0.05); while the MDA concentrations was decreased
in these groups (p< 0.05). This is the 1rst report showing the pharmacological activity of Rhizophora mangle
(L.), modifying the REDOX balance of the wounds evolution with benefit for the healing process due to the
increasing of the antioxidant biomarkers and the decreasing pro-oxidant indicators in the wound.
PPP-21
UPDATE OF EXPERIMENTAL BIOPHYSICAL AND RHEOLOGY PROPERTIES OF NATURALS LUNG
SURFACTANTS.
Odalys B.1,2, Cruz A2, Morales I3, Faure R1 and Pérez-Gil J2
1
Group of Chemistry, Pharmacology and Toxicology, National Centre for Animal and Plant Helath Cuba, CENSA.
Apdo. 10, San José de las Lajas, La Habana, Cuba.
2
Group of Chemistry, Pharmacology and Toxicology, National Centre for Animal and Plant Health, CENSA, La
Habana, Cuba
3
Centro de Investigaciones y Desarrollo de Medicamentos, CIDEM, Ciudad de la Habana, CP 10400, Cuba.
Introduction: Regardless of the broad clinical use of natural surfactant preparations available today in
Respiratory Distress Syndrome in newborn, the basic biophysical research reveals important differences in
composition, surface activity and viscosity between these one and therefore in clinical effects. Recently has
been suggesting the relation between rheology and biophysical behavior and therapeutic effect of commercial
lung surfactant. The objective of this work was to carry out biophysical and rheology characterization of
SURFACEN®, a natural lung surfactant obtained at CENSA, Cuba, in comparison with other commercial
preparation. Material and Methods: Biophysical characterization of SURFACEN® was carried on by the
Wilhelmy Balance; rheology properties included viscosity and particle size by rheometer and particle counter,
respectively. Results: Current results showed an updating according to the models used bringing a better
knowledge on the role of the main components of pulmonary surfactants as a further reference evaluating the
biochemical composition of different preparations of exogenous natural pulmonary surfactant currently in use
and their apparent pharmacological effect.
PPP-22
IN VITRO AND IN VIVO ANTI-TUMOUR EFFECT OF THE AQUEOUS EXTRACT OF THE MANGIFERA
INDICA L. STEM BARK EXTRACT AND ITS MAIN COMPONENT MANGIFERIN.”
Magariño Y1, López O2, Coipel L1 and Delgado R3.
Center for Pharmaceutical Chemistry. 200 at 21 Ave, Atabey, Playa, Havana City.
2
Center for Research of Biological Evaluations. 222 at 25 Ave, La Coronela, Lisa, Havana City
3
National Coordinator Center for Clinical Trial, 200 at 21 Ave, Atabey, Play, Havana City.
1
Lung cancer is the leading cause of cancer death in the world. As a result the search for new treatment
products from natural sources constitute an important task of modern pharmacology. The present study
evaluated the anti-tumour effect of the aqueous extract of the Mangifera indica L. stem bark extract (MISBE) and
of its main component mangiferin, on the tumor cell lines 3LLD/122 and U1906. In anti-proliferative studies,
MISBE showed little toxicity on the 3LLD/122 cell line, with a IC50 of ≈ 216.7 µg/ml, and the mangiferin did not
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exhibit cytotoxic activity; whereas on the U1906 cell line MISBE, as mangiferina, did not exhibit significant
cytotoxic activity.
It was determined, using flow cytometry 24 hours after treatment, that MISBE, as mangiferina, did not induce
apoptotic mechanisms of death; nevertheless, both products did induce necrotic mechanisms. 48 hours after
treatment, MISBE was able to induce apoptotic mechanisms. It was obtained in in vivo studies that MISBE (25,
50 and 100mg/Kg) and mangiferin (5, 10 and 20mg/Kg) inhibited significantly the tumor growth in the dorsal line
of mice C57BL/6, 24 days after the challenge. In the treatment with both products there was a higher percentage
survival rate 45 days after the challenge. These results show that both products could be considered for use
concurrently with conventional cancer treatments.
PPP-23
EVALUATION IN VITRO OF THE RADIOPROTECTIVE EFFECT OF THE AQUEOUS EXTRACT OF
MANGIFERA INDICA L. (VIMANG).
Rosario L1, Rodeiro I1, Almeida E2, Leyva O1, Alonso A2, Delgado R3, Rodriguez Y1.
1-Centro de Química-Biomolecular (CQB)
2-Centro de Aplicación y Desarrollo de la Energía Nuclear (CEADEN).
3-Centro Nacional Coordinador de Ensayos Clínicos (CENCEC)
The ionizing radiations is used with success in the treatment against the cancer and other illnesses. For it, a
growing interest exists in developing new products with radioprotective character that diminish the adverse
effects of the radiotherapy. The aminotioles (as the amifostina) are the agents that more they have been used
with this end. However, the continuous employment of this type of compounds causes severe secondary effects.
For this reason, the current tendency is focused to obtaining radioprotective agents from natural sources.
The natural product, Vimang, that one obtains from the bark of Mangifera indica L. constitutes an interesting
candidate for the evaluation as radioprotective if one keeps in mind that the pharmacological studies carried out
until the moment, corroborate its effectiveness like antinflamatorio, analgesic, inmunomodulador and
characteristic antioxidante all described for an ideal radioprotector.
The execution of the SOS Chromotest indicated that the extract is not genotoxic in the analyzed range of
concentrations (50, 250, 500 and 1000 µg/mL); in presence or not of metabolic activation. Additionally, it was
determined that the Vimang presents character radioprotector on the genetic material, when it is used to a same
concentration or superior to 250 µg/mL. Evaluations carried out in human red blood cells indicated that the
evaluated product inhibits the lipid peroxidation, when it is used between 500 and 1000 µg/mL and it diminishes
the damages caused to the mitochondrion and its enzymes, when being used in a similar range of
concentrations. These results demonstrate that the extract is able to protect the cellular structure at several
levels: nuclear material, cellular membranes and mitochondrial enzymatic systems, of the damages induced by
the gamma radiation.
PPP 24
PROTECTIVE EFFECT OF THE Phyllanthus orbicularis, HBK AQUEOUS EXTRACT ON HUMAN CELLS
AGAINST THE DAMAGE INDUCED BY UVB RADIATION.
Vernhes M 1, González-Pumariega M 2, Andrade L 3, Passaglia A 3, Moreno de Lima-Bessa K 3, Menck C F
and Sánchez-Lamar A 2 .
1
Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear (CEADEN), Apartado postal 6122, Calle 30. #502, e/
5ta y 7ma, Miramar, Playa, Ciudad de la Habana, Cuba. E-mail: [email protected]
2
Facultad de Biología de la Universidad de la Habana. Calle 25 e/ I y J, Vedado, Ciudad de la Habana, Cuba.
3
Department of Microbiology, Institute of Biomedical Science, University of Sao Paulo, Av. Prof Lineu Prestes,
1374, Sao Paulo, SP, 05508-900, Brazil.
3
Over recent years, the incidence of various diseases and disorders related to solar ultraviolet (UV) radiation has
increased alarmingly. UVB radiation represents one of the most important environmental factors affecting
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humans. The main DNA lesions induced by UVB light, cyclobutane pyrimidine dimers (CPDs) and 6-4
photoproducts (6-4PPs), are considered the major causes of apoptosis and mutagenesis induction. Also this
mutagen increases cellular reactive oxygen species levels (ROS).
One approach to protect human skin against UVB harmful effects is the use of natural products as
photoprotectors. In this sense, the aim of the present investigation was to evaluate Phyllanthus orbicularis HBK
extract properties in the protection of human cells against UVB light-induced damage. DNA repair proficient and
deficient fibroblasts (MRC5-SV and XP4PA) were used as experimental models. The damaging effects of UV
were evaluated through clonogenic cell survival and apoptosis induction, while DNA repair was determined by
the removal of cyclobutane pyrimidine dimers. The results indicated that an extract of P.orbicularis protects
human cells against UVB light-induced damage in concentrations where no light absorption is observed; this
protection being mainly related to positive modulation of cell ability to repair this type of DNA damage.
PPP-25
THE PROTECTIVE CAPACITY OF Cymbopogon citratus (DC) Staff EXTRACT AGAINST THE DAMAGE
INDUCED BY ULTRAVIOLET RADIATION
González-Púmariega, M1, Vernhes, M2, Menck C F3, Sánchez-Lamar, A1.
Laboratorio de Genética Toxicológica, Departamento de Biología Vegetal, Facultad de Biología, Universidad de
la Habana, Apartado Postal 10 400, Calle 25, # 455, e/ I y J, Vedado , Plaza de la Revolución, C. Habana,
Cuba. Fax: 537 8321321.
2
Departamento de Radiobiología, (CEADEN) Apartado Postal 6122, Calle 30, # 502, e/5ta y 7ma, Miramar,
Playa, C. Habana, Cuba. Fax: 537 2041188.
3
Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu
Prestes, 1374 Ed. Biomédicas 2, São Paulo, SP, Brazil. Fax: 05508-900.
1
Cellular DNA has been identified as a target for most of the biological effects of UV radiation, and the induction
of photodamage is considered as the initiating step of disorders, including sunburn, photoaging and
photocarcinogenisis. Adequate chemoprotection by means of vegetable extracts is a new strategie essential to
control harmful overexposure to solar UV. In this study we evaluated the photoprotector capacity of aqueous
extract of Cymbopogon citratus (DC) Staff against cyclobutane pyrimidine dimers (CPD), the most frequent
photolesions induced by UV radiation. We use supercoiled circular plasmid DNA model to evaluate the
photoprotection by the extracts before, during, and after having irradiated with UVC 200J/m2. These studies
showed that C. citratus presents photoprotector properties against genotoxicity of UV that could be sustained by
different action mechanisms.
PPP-26
ANTIPROTOZOAL BIOMONITORED FRACTIONATION OF THE BRAZILIAN PLANTS EXTRACTS
HIMATANTHUS SUCUUBA AND CAMPSIANDRA COMOSA
Duarte-Almeida JM1, Tempone AG2, Reimão, JQ2, Rodrigues E1, Carlini EA1.
Psicobiology Department UNIFESP, São Paulo, Brazil.
2
Applied Toxinology Laboratory, Instituto Adolfo Lutz, São Paulo, Brazil. E-mail. [email protected].
1
Leishmania (L.) chagasi is the causative agent of Visceral Leishmaniasis, one of the major problems of public
health in several tropical countries. Plants have been a promising source of secondary metabolites with potent
antiparasitic activity. In this work we report the antiprotozoal activities of Himatanthus sucuuba (HS) and
Campsiandra comosa (CC), as well as determination of main class secondary metabolites. HS and CC bark
were collected from Amazonian forest in Brazil, dried and extracted with methanol 80%. Extracts were assayed
against Leishmania (L.) chagasi according to Tempone et al. (2008). The phytochemical screening by thin layer
chromatography showed that HS presents phenolic compounds (tannin, phenolic acids and flavonoids),
triterpenoids and iridoids, and CC showed only phenolic compounds (tannin and phenolic acids). CC extract
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showed significant antileishmanial activity against the promastigote form of Leishmania (L.) chagasi at 500
µg/mL. This extract was fractioned and water, butanol, ethyl acetate and hexane fractions were tested for
bioactivity. The ethyl acetate and water fractions presented a considerable antileishmanial activity, killing 100%
of promastigotes at 300 µg/mL. These two fractions were active and will be fractionated in high performance
liquid chromatography for isolation of the active compounds.
Acknowledgements: PRODOC/CAPES/CNPq.
References: Tempone AG, Sartorelli P, Teixeira D, Prado FO, Calixto IA, Lorenzi H, Melhem MS. Brazilian flora
extracts as source of novel antileishmanial and antifungal compounds. Mem Inst Oswaldo Cruz. 2008
Aug;103(5):443-9.
PPP-27
SEARCH FOR NEW SECONDARY METABOLITES WITH BIOLOGICAL ACTIVITY FROM ENDOPHYTIC
FUNGI.
Hidalgo PI1, Anke T2, Meffert A2, Medina RP1, Peñate L3, García MR1, Casanova M1, Enríquez V1
Centro de Bioactivos Químicos, Universidad Central de Las Villas, Carretera a Camajuaní Km 5 1/2, Santa
Clara, Cuba.
2
Department of Biotechnology, Technical University of Kaiserslautern, Paul-Ehrlich-Str. 23, 67663
Kaiserslautern, Germany.
3
Departamento de Biología, Universidad Central de Las Villas, Carretera a Camajuaní Km 5 1/2, Santa Clara,
Cuba.
1
Endophytic microorganisms reside in the tissues of the host plant and do so in a variety of relationships ranging
from mutualism to pathogenic. Endophytes may contribute to their host plant by producing a plethora of
substances that provide protection and survival value to the plant. Recently, these compounds, once isolated
and characterized, may also have potential for use in modern medicine, agriculture, and industry. Endophytic
fungi from 10 plant species were isolated. Identification was based on colony morphology, hyphal morphology
and reproductive structures when present. Liquid medium were inoculated with fungi culture and were allowed to
grow at 25°C until the glucose level was 0. The filtered broth and the mycelium were extracted with organic
solvents. Chromatography methods were used to obtain fractions and purify the antimicrobial compounds.
Antimicrobial activity of extracts was evaluated again bacteria and fungi using diffusion agar method. 42
isolations of fungi were obtained. The predominant endophyte genera found in all plants were Alternaria,
Pestalotia and Phomopsis. Alternaria and Phompsis produced a variety of antimicrobial compound. Extracts
from the filtered broth showed greater antimicrobial activity than extracts made from the mycelium. Endophytic
fungi studied showed a varied production of secondary metabolites with antimicrobial activity.
PPP-28
ANTILEISHMANIAL ACTIVITY OF BIXA ORELLANA.
García M1, Monzote L1, Montalvo AM1, Scull R2.
Instituto de Medicina Tropical “Pedro Kourí” (IPK).
2
Instituto de Farmacia y Alimentos (Universidad de La Habana).
1
Leishmaniasis comprises a group of infectious diseases caused by organisms hemoflagellates of the genus
Leishmania. Actually, leishmaniasis is prevalent in 88 tropical countries throughout the world and it estimated
that 1.5 million to 2 million reports of new cases occurs annually. Control of leishmaniasis relies on
chemotherapy, but the availability and effect of conventional drugs are limited. Although exist some drugs, these
are toxic, expensive and require long-term course of therapy. The Program of Tropical Diseases of WHO
considered the investigation of plant as essential priority for the treatment of Leishmania. In the present study
the antileishmanial activity of alcoholic extract of Bixa orellana against Leishmania amazonensis was evaluated.
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The activity against promastigote was assessment by the alkaline phospathase test, the citotoxicity was
determined by a tetrazolium salt, and the amastigotes test was performed by counting. In the in vivo assays
female of BALB/c mice was used. In the in vitro assays the extract showed CI50 values of 22.05 y 9.1 µg/mL
against promatigotes and amastigotes respectively, while a moderate toxicity on macrophages from BALB/c
mice was observed. At a dose of 100 mg/Kg/day the extract was effective when administered during 15 days by
intraperitoneal route to BALB/c mice experimentally infected. Este estudio evidencia la importancia de los
productos naturales como fuentes potenciales para el desarrollo de nuevas drogas antileishmaniales. This study
supports the importance of natural products as potential sources in the searching for new antileishmanial drugs.
PPP-29
CHEMISTRY COMPOSITION, CITOTOXICITY AND ANTILEISHMANIAL ACTIVITY OF THE ESSENTIAL OIL
FROM PIPER AURITUM.
Monzote L1, García M1, Scull R2, Miranda M2
Departamento de Parasitología, Instituto de Medicina Tropical “Pedro Kourí”, Cuba.
2
Instituto de Farmacia y Alimentos, Universidad de La Habana, Cuba.
1
Leishmaniasis defines a group of endemic diseases caused by species of the genus Leishmania. Due to the
high death and morbidity rates the World Health Organisation has considered it as a major tropical disease. New
anti-leishmanial drug discovery is a need as currently available therapy includes only parenterally administrable
drugs, requires hospitalisation for long treatment periods, is expensive, is potentially toxic and parasite
resistance has emerged as a further problem. About 60% of patients use traditional medicine and that have
served as source to search for new therapy choices. In the present work we tested the efficacy of essential oils
extracted from Piper auritum (commonly known as “caisimón”) on Leishmania. The oil extract was characterised
by gas chromatography – mass spectrometry. Major component was safrole (87%). The oil of Piper was active
against promastigotes of L. major, L. mexicana, L. braziliensis y L. donovani, with an 50% inhibitory
concentration (IC50) below to 100 µg/ml. The IC50 against amastigotes of L. donovani was of 40 µg/ml and
against peritoneal macrophages from BALB/c was of 106 µg/ml, showing the better selectivity index. These
results show the potential of essential oils from Piper auritum as anti-leishmanial therapy.
PPP-30
EFFECT OF THE SEASONAL VARIABILITY IN THE COMPOSITION AND THE BIOLOGICAL ACTIVITY OF
THE ESSENTIAL OIL OF SIPARUNA BIFIDA.
Saavedra G1, Poka D1, Ruiz G2.
Centro de Tecnología Agroindustrial, UMSS, Cochabamba - Bolivia. [email protected]
2
Instituto de Investigaciones Fármaco - Bioquímicas, UMSA, La Paz - Bolivia.
1
Between the natural resources that the Bolivian flora has, there are poorly known and/or exploited vegetal
species, such as aromatic, medicinal and insecticide; those that could be potential sources of raw materials for
the useful product preparation for the humanity, contributing to the knowledge of native flora and its valuation.
In previous research we studied the antibacterial, antifungal and antiparasitic activity of plant species belonging
to the SIPARUNACEAE family and whereas Siparuna bífida is a potentially exploitable specie since its essential
oil showed an interesting antiparasitic activity, in this work it was considered to study the effect caused by
seasonal variability in the composition and biological activity that presents the essence of the Siparuna bifida
from the Valley of the Sacta-Carrasco (Cochabamba-Bolivia).
Random samples were collected from the aerial parts of plants in the months of October (full flowering) and
December (fruiting). Essential oils were obtained from fresh material by the water steam extraction method
(Hidrodiffusion), reaching yields between 0.1 and 0.3% on wet basis.
GC and GC-MS analysis allowed the identification of the majority components of essential oils, those that were
put under an evaluation “in Vitro” to determine their antiparasitic activity in Chagas (Tripanosoma cruzi), Malaria
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(Plasmodium falciparum sensible and resistant to the cloroquine) and Leishmaniasis (Leishmania amazonensis,
Leishmania brasiliensis and Leishmania donovani).
The chromatographic profiles of the essences obtained in both months are similar, being basically represented
by terpénics hydrocarbons (56%) and oxygenated terpenes (44%). Quantitative variations in single some
components are observed such as: biciclogermacrene (15.0 to 7.3%) and -bisabolene (19.2 to 15.8%).
Although the essential oils obtained at the time of flowering and fruiting, present activity before the 3 studied
parasites, the essential oil obtained from flowering stage exhibits a greater activity in front of the parasites that
produce Malaria and Chagas. The essence of better quality would be obtained with plants in full bloom; due to
these variations it would be interesting for future works, the analysis of the ontogenic effect on the composition
of the essential oil and therefore in the biological activity of the vegetal specie.
PPP-31
SEARCH FOR ANTIVIRAL ACTIVITY OF THE PLANT AGERATINA HAVANENSIS AGAINST DNA AND RNA
VIRUSES
Acosta M1, Álvarez AL1, Sarmiento L2, García T3, Acosta I4, Spengler I3, Roque A1, del Barrio G1*.
Grupo de Antivirales Naturales. Facultad de Biología. Universidad de La Habana. Calle 25 Nro.455 e/ J e I,
Vedado, Plaza de la Revolución, Ciudad de La Habana, Cuba.
2
Laboratorio de Enterovirus. Instituto de Medicina Tropical Pedro Kourí. Autopista Novia del Mediodía, Km 6 ½ ,
La Lisa, Ciudad de La Habana, Cuba
3
Grupo de Estudio de Productos Naturales. Facultad de Química. Universidad de La Habana.
4
Instituto de Investigaciones Forestales. Ciudad de La Habana.
* [email protected]
1
The high toxicity of synthetic antiviral compounds, the continuous spread of resistant viruses mutants and the
lack of effective vaccines against many viruses highlight the need for new inhibitors. In this scenario, the search
for antiviral metabolites from natural sources represents a promising strategy. In this work, the chemical
composition and antiviral activity of extracts from Ageratina havanenis (Family Asteraceae) stems and leaves
were investigated.
Citotoxicity of the test extracts were assessed by means of the MTT colorimetric assay and antiviral activity was
determined as a function of viral-induced cytopathic effect (CPE) reduction under a light microscope. Viruses
used in this study included Poliovirus 1, Coxsackievirus A16, Coxsackievirus B5, Echovirus 9, Rabbit vesivirus
(RNA viruses) and Herpes Simplex Virus type 2 (DNA virus).
Ethanol extract from stems and ethanol extract from leaves were effective against Poliovirus 1 (selectivity
indexes 3.2 and 2.1, respectively) and HSV-2 (SI 2.6 and 2.2 respectively), while ethanol extract from leaves
was further active against Coxsackievirus B5 and Coxsackievirus A16 (SI 3.2 and 3.3 respectively). The higher
inhibitory activity was recorded for the ethanol extract from stems against Rabbit vesivirus (SI= 4.7).
Triterpenoids, steroids, flavonoids and glycosides were the major constituents detected in A. havanensis
extracts.
PPP-32
AGERATINA HAVANENSIS HBK EXTRACTS INHIBIT RABBIT VESIVIRUS IN VITRO REPLICATION
del Barrio G1*, Álvarez A.L1, Acosta M1, Spengler I2, García, T2 y F. Parra3.
Departamento de Microbiología y Virología. Facultad de Biología. Universidad de La Habana. Calle 25 #455
Vedado, Plaza de La Revolución. 10400. Cuba;
3
Departamento de Productos Naturales. Facultad de Quimica. Universidad de La Habana. Plaza de La
Revolución. 10400. Cuba;
3
Departamento de Bioquímica y Biología Molecular. Universidad de Oviedo. Edificio Santiago Gascón.
Campus El Cristo. Oviedo. Asturias. 33006. España. * [email protected]
1
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Ageratina havanensis (Asteraceae) inhibitory activity against DNA and RNA viruses has previously been studied
by our group. In this work, extracts obtained from stem and leaves from this plant, with different solvents
(ethanol: EtOH, n-butanol: nBtOH, ethyl acetate: AcOEt), were screened for inhibitory activity against Rabbit
Vesivirus (RaV) (Family Caliciviridae). 1
Citotoxicity of the test extracts were assessed by means of the MTT colorimetric assay and antiviral activity was
determined as a function of viral-induced cytopathic effect (CPE) reduction under a light microscope. The time of
inhibition was studied using a time-of-addition experiment. Virucidal assays were carried out in order to assess
the extracellular virus-inactivating capacity of the plant extracts.
Selectivity indexes (SI) ranged from 2.3 to 4.7. The strongest inhibition was recorded for extracts obtained from
stems. A. havanensis affected intracellular viral replication processes rather than early events, as it could be
demonstrated by time-of-addition assay. No virucidal effect was found for the plant against this virus, since viral
suspensions remained infective after treatment with 8-fold EC50 extract concentration, no matter the time of
exposure employed. The mechanisms underlying intracellular viral inhibition merit further research.
References:
1
Martin-Alonso,J.M, Skilling, D.E., Gonzalez Molleda,L., del Barrio, G., Machi, A., Matson D.O., Iversen P.L.,
Smith,A.W., Parra,F. 2005. Isolation and characterization of a new Vesivirus from rabbits. Virology 337, 373383.
PPP-33
PHYLLANTHUS ORBICULARIS INHIBITORY ACTIVITY AGAINST HERPES SIMPLEX VIRUSES AND
HUMAN CYTOMEGALOVIRUS: AN APPROACH TO ANTIVIRAL MECHANISMS.
Álvarez AL1, Kourí V 2, Martínez PA 2, Parra F 3, del Barrio G1 *
Grupo de Antivirales Naturales. Facultad de Biologia. Universidad de La Habana. Calle 25# 455. Vedado. Plaza
de La Revolución. Ciudad de La Habana, Cuba.
2
Laboratorio de Infecciones de Transmisión Sexual. Departamento de Virología. Instituto de Medicina Tropical
Pedro Kourí. Autopista Novia del Mediodía Km 6½. La Lisa. Ciudad de La Habana, Cuba.
3
Instituto Universitario de Biotecnología de Asturias. Departamento de Bioquímica y Biología Molecular.
Universidad de Oviedo. Asturias, España.
* [email protected]
1
The family Herpesviridae comprises several viral species pathogens to humans, responsible for a wide spectrum
of clinical symptoms and high morbidity rates. The high toxicity of synthetic anti herpes compounds and the lack
of an efficacious vaccine have emphasized the search for new inhibitors among natural products, particularly
from plants.
In this work, the antiviral activity of Phyllanthus orbicularis aqueous extract was evaluated in vitro against a virus
panel including acyclovir (ACV)-sensible and resistant HSV-1 and HSV-2 references strains and clinical isolates,
as well as against HCMV reference strain AD169. Citotoxicity of the test extract was assessed by means of the
MTT colorimetric assay and antiviral activity was determined as a function of viral-induced cytopathic effect
(CPE) reduction under a light microscope. The time of inhibition was studied using a time-of-addition experiment
and virucidal assays were carried out in order to assess the extracellular virus-inactivating capacity of the plant
extract. Anti HCMV activity was further confirmed by a real time PCR assay.
P. orbicularis was effective against all studied viruses and selectivity indexes (SI) ranged from 8.7 and 40.8. The
product was effective from the very early events of viral infection and a strong virucidal effect was achieved
since viral suspensions titres decreased by 1 log10 or more after treatment with the extract. In the presence of P.
orbicularis, the concentrations of HCMV viral DNA in infected-cultures supernatants were at least 5-fold lower
when compared to that of untreated infected cells.
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PPP-34
IN VITRO ANTIVIRAL ACTIVITY OF MEDICINAL PLANTS AGAINST HEPATITIS B VIRUS (HBV).
Roque A, González R, Morier L, Rodríguez LA, del Barrio, G
Department of Microbiology and Virology. Faculty of Biology. Calle 25 #455 e/ J e I. Vedado. Havana. Cuba. Email: [email protected]
Medicinal plants have been widely used for the treatment of infectious diseases. In Cuba several plants are
commercially available. In this work the antiviral properties of plants extracts derived from three medicinal plants
used in Cuba: Calendula officinalis L., Psidium guajava L. and Eucaliptus spp., against the hepatitis B virus were
evaluated at subtoxic concentrations in the in vitro PLC/PRF/5 system or Alexander cells, a cell line expressing
constitutively the virus surface antigen (HBsAg). The cell viability was measured by the MTT method and the
mean cytotoxic concentration values (CC50) were calculated. The antiviral activity was considered as the ability
to inhibit the HBsAg production during 48 hours of treatment and expressed as IC50. The relation between CC50
and IC50 represented the Selective Index (SI). Eucalyptus spp. showed a lower toxicity in the cells, followed by
Psidium guajava L. and finally Calendula officinalis that had a much higher toxicity than the previous extracts.
The data obtained showed an inhibitory activity in the case of the Eucalyptus extract (IS=25.21) higher than the
guava extract (IS=6.70), whereas Calendula did not show any inhibition in the assayed concentrations, which
proves the absence of the searched activity in this extract. The behaviour of the HBsAg intracellular produced
after the treatment of the cells with Eucalyptus and guava is similar to those obtained for the extracellular
antigen. These results suggest new possibilities to explore in the search of therapeutic alternatives against
hepatitis B virus infection.
PPP-35
THE COEFFICIENT OF SPECIFICITY OF INHIBITION AgsVBH IN SPECIES CUBAN PHYLLANTHUS.
Chevalier P1, León M1, Hernández M1, Cedeño P2, Vidal M3
Cathedra of Natural and Traditional Medicine of the ISCM "Carlos J Finlay Camagüey"
2
Laboratory of Clinical Trials of Blood Bank Provincial Camagüey.
3
Faculty of Health Technology, Municipio 10 de Octubre, Havana City.
1
Introduction. The antiviral properties of the species of Phyllanthus are attributed to the presence of phenol type
compounds. These inhibit the DNA polymerase and reverse transcriptase. The study examines the correlation
between the concentration of phenolic compounds and the reduction of surface antigen of hepatitis B viral
(AgsVBH) cuban species with reported antiviral action. Materials and Methods. Aqueous extractions were made
of plant material. Were measured concentrations of phenolic compounds c(PhC) by colorimetric method and
were standardized extracts concentrations (0.1 to 0.7) mg / ml. To each extract was determined the activity by
reducing of AgsVBH in serums from seropositive patients (ΛAgsVBH) for the interval (1/800 to 1/51 200) using
the ELISA technique. Correlated pairs by the method of least squares (R). The correlation coefficients obtained
were subjected to analysis of variance. Results. The increase in c(PhC) is inversely and linearly proportional to
the reduction of antigen (ΛAgsVBH), because (R) 2 are in ranges from 67.73 to 51.12 with an analysis of
variance results P < 0.05. This proves the metabolite-bioactivity relationship and define the coefficient of
inhibition (Cinh) as the slope of the straight line correlation (ΛAgsVBH) vs c (CPhC). Conclusions The coefficient
of specificity of inhibition (Cinh) AgsVHB lets compare the magnitude of the effectiveness of antiviral action in
vitro of species of Phyllanthus. The highest value corresponds to the root of P. chamaecristiodes.
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PPP-36
ANTIBACTERIAL ACTIVITY OF FOUR NATURAL PRODUCTS.
Echemendía O, Almora E,Fidalgo O, Monteagudo R,Bolaños G,Lago V.
Instituto Finlay. Centro de Investigación-Desarrollo-Producción de Vacunas, Ave 27 No.19805, La Lisa,
Ap.16017. Cod. 11600. La Habana, Cuba E mail: [email protected] Fax: 53 (7) 208-60-75
Many natural products possess antibacterial activity that which they can be used as therapeutic alternative
because of the appearance of antibiotic-resistance to antibiotics mutant bacteria The aim of the present study
was evaluate the antibacterial activity of 2 oils essentials, one coming from Albahaca and another of Citronela
and alcoholic and aqueous extractions (decoction and infusion leaves) of Guava (Psidium Guajaba) and Anamú
(Petiveria Alliacea) against of the references strains Escherichia coli (ATCC25922), Staphylococcus aureus
(ATCC 29213), Cándida albicans (ATCC 10323). The microorganisms were adjusted 0.5 Mc Farland. The
diffusion method was used according the National Committee for Clinical Laboratory Standar Guideliness
(NCCLS), using medium Triptona Soya Agar. The inoculated plates were incubated 24 hours, to 37 OC and
humid atmosphere with 5% of CO2. The essential oils were diluted from pure to 10-6. The watery and alcoholic
extracts of Anamú and Guava were carried out double dilutions from 1:2 until 1:64. The aqueous and alcoholic
extracts of Anamú did not present antibacterial activity. The aqueous and alcoholic extracts of the Guava
presented activity antibacterial front E. coli and S. aureus. The essential oils presented antibacterial activity in
front of S. aureus. These products can be used in formulations antibacterial for the treatment against these
infections related with bacteria.
PPP-37
ANTIMICROBIAL EVALUATION OF TOTAL EXTRACTS FROM LEAVES OF PETIVERIA ALLIACEA L.
(ANAMÚ).
Ochoa AP1, Marín JM1, Mujawimana RJ1, Tamayo KRG1, González ZG2.
Pharmacy Department1 and Center of Study of Industrial Biotechnology2: Faculty of Naturals Sciences.
Universidad de Oriente.
Patricio Lumumba s/n. Santiago de Cuba. Cuba. E-mail: [email protected]
Currently investigations in the world look for new and effective proposals for the management of bacterial
infections. In studies already carried out with Petiveria alliacea L., it was observed that plant extracts do not
undergo microbial contamination when they are exposed to uncontrolled storage conditions. This fact led to the
antimicrobial evaluation of 8 total extracts of the leaf: two of them in the ratio of 1:4 were prepared from the dried
leaf and ethanol 30% as the solvent; the remaining 6 were prepared using fresh leaves and ethanol 30%, 80%
and isopropylic alchohol as solvents, in the ratio of 1:4 and 1:6 respectively. For the antimicrobial evaluation, the
Kirby-Bauer disk diffusion method was opted using the following different strains of reference: Staphylococcus
aureus, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa and Candida albicans. The results
showed that only antibacterial activity was obtained with a zone of inhibition of more than 6mm. in 3 of the soft
extracts: 2 with isopropylic alchohol (1:4 and 1:6) and 1 with ethanol 80%(1:6), against Staphylococcus aureus
and Escherichia coli; thereby corroborating that heat and drying of the plant destroys the components with
antimicrobial activity in anamú1 and that non-polar solvents are more adecuate for the extraction process of the
components with the mentioned activity. The soft extracts with isopropylic alchohol possess higher antibacterial
activity. Antifungal activity was not obtained on the assayed extracts.
1. Seokwon K., Roman K., Rabi A. M. Antibacterial and antifungal activity of sulfur-containing compounds from
Petiveria alliacea L. Journal of Ethnopharmacology 2006, (104): 188–192.
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PPP-38
CITOTOXICITY OF FRACTIONS FROM OF THE ESPECIE ERYTHROXYLUM MINOTIFOLIUM ON TWO
CELLS LINES.
Almora E1, Monteagudo R1, Fidalgo O1, Echemendía O1, Bolaños G1, García M1, Lago V1, González J A2.
Instituto Finlay. Centro de Investigación-Desarrollo-Producción de Vacunas. Ave 27 No. 19805, La Lisa, A. P.
16017. Cod. 11600. La Habana, Cuba.
Correo electrónico: [email protected] Teléfonos: 271-8221, 271-8177
2
Centro de Química Farmacéutica (CQF), La Habana, Cuba
1
The genus Erythroxylum contains species with potent antiviral, antibacterial and fungical activity. Among the
chemical groups have been isolated alkaloids, flavonoids, triterpenes. The first stage in the study of any product
as a potential antiviral is a quantitative assay to assess their effects on cells or cytotoxicity, expressed as the
concentration value that causes cell damage evident at the 50% of the treated cultures cytotoxic concentration
median(CC50). Knowing this value allows subsequent use or lower concentration for testing antiviral activity. In
our work, we evaluated the cytotoxicity of 11 chemical fractions from of the specie Erythroxylum minutifoluim.
The experimental design consisted of determining the Cytotoxicity Concentration Median (CC50) on Vero and
FPH cell lines. The effect was determined by the calorimetric method of MTT. The fractions EM-C and EM-CM at
concentrations of 778.05 µg/mL and 792.63 µg/mL, respectively, showed a cellular viability higher than 90% in
the Vero cell line and the concentrations of 863.72 µg/mL and 898.06 µg/mL in the FPH cell line. While for the
Fractions P-FA, FA, FA-1, FA-2, FP, EX1, EX2, EX3 and P remained above 90% until a concentration of 1000
µg/mL. The behavior of the variables in the 11 fractions, showed no statistically significant differences between
the two cell lines for p> 0.05, for that reason it possibility use both cell lines for antiviral assay . Fractions P-FA,
FA, FA-1, FA-2, FP, P, EX1, EX2 and EX3 were not toxic up to a concentration of 1000 µg/mL in the cell lines
used.
Keywords: citotoxicity, specie Erythroxylum minutifolium
PPP-39
INCREASED INSULIN SECRETION IN RAT BETA CELLS TREATED WITH LOW MOLECULAR WEIGHT
FRACTIONS FROM Physalia physalis.
Carlos Manlio Díaz García1, Carmen Sánchez Soto2, Deyanira Fuentes3, Adela Rodríguez3, Acela
Pedroso4, Marcia Hiriart Urdanivia2, Olga Castañeda Pasarón1.
1
Dpto. Biología Animal y Humana, Facultad de Biología, Universidad de La Habana
2
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México
3
Instituto de Química, Universidad Nacional Autónoma de México
4
Instituto de Endocrinología, MINSAP
Introduction: The Phylum Cnidaria has a great biodiversity of poisonous organisms, whose mechanisms include
the modulation of ion channels. Its study is a wide field of research since those toxins are commonly very
specific and potent, and therefore usefull tools in electrophysiology and promising drugs for therapeutics.
Subjects: The aims of this work were to purify the Physalia physalis crude extract and to determine the effects of
the low molecular weight fractions on a model of insulin secretion of beta cells from Wistar rats pancreas.
Materials and Methods: The Physalia physalis crude extract were purified by a gel filtration using a Superdex
200 HiLoad 60/30 column in an AKTA-FPLC system (Amersham Pharmacia Biotech). Mass spectrometry
MALDI-TOF (Bruker Daltonics) and RP-HPLC (Ultrasphere-DABS C18 column, Agilent 1100 system) were
assessed to each fraction collected from gel filtration. The biological activity of low molecular weight fractions
was assessed in a reverse haemolitic plaque assay on primary cultures of pancreatic beta cells from male
Wistar rats. Results: Fractions IV and V from gel filtration increased the secretion index at both glucose levels
(5.6 mM and 16.5 mM) whereas at low glucose they exert their effects by increasing the inmunoplaque area but
also recruiting a population of non secretory beta cells. Conclusions: The venom of Physalia physalis contains
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bioactive peptides capable to enhance the secretory behavior of pancreatic beta cells from male Wistar rats, and
this effect occurs at basal and stimulating levels of glucose in the culture media.
CARTELES FARMACOLOGIA CLINICA / POSTERS CLINICAL PHARMACOLOGY.
PCP-01
FERMENTED RICE IN PATIENTS SUFFERING ADVERSE REACTION TO SYNTHETIC HMG-CoA
REDUCTASE INHIBITORS: TREAMENT OF A SERIES OF 24 PATIENTS WITH FERMENTED RED RICE
Gori L, Mascherini V, Firenzuoli F
Clinical Center of Natural Medicine (Dir.: F. Firenzuoli, MD), S.Giuseppe Hospital, . Az USL 11 Empoli, I TALY
A frequent adverse reaction of HMG-CoA reductase inhibitors is significant muscle toxicity and elevations of liver
transaminases (1). In our database we have some patients that asked an alternative treatment for high
cholesterolemia after suspending HMG-CoA reductase inhibitors for adverse reactions to this type of synthetic
drug. We treated these patients with extracts of fermented red rice, whose main active components are
policosanols and monacolin, a natural analogue of lovastatin that can give rise to the same type of adverse
reactions (2,3), nevertheless in some patients it can be a safe and effective treatment. We administered to our
series of patients extracts of fermented red rice tritrated in monacolin for at least 12 months; they underwent
every three months a biochemical and clinical control.We observed a reduction of cholesterolemia and no
adverse event.
Bibliography: 1. BMC Clinical Pharmacology 2009; 9:6. 2. Br J Clin Pharmacol. 2008;66(4):572-4. 3. Firenzuoli
F. Fitoterapia. Elsevier ed, Milan, 2008.
PCP-02
ANTI-INFLAMMATORY EFFECT OF AN EXTRACT OF MANGIFERA INDICA L. (VIMANG®) IN PATIENTS
WITH CHRONIC DERMATITIS.
Guevara M*1, Pérez T2, Calderón B2, Perdomo J2, Morales C1, Garrido G1.
1
Clinical Pharmacology Department. National Coordinating Centre of Clinical Trials. 200 street and 21 avenue,
Atabey, Postal Code 11600. Havana City. Cuba.
2
Dermatology Service. Carlos J. Finlay Hospital. 114 street and 31 avenue. CP 11500. Havana City. Cuba. E
mail: [email protected]
Introduction: In today context we see an increasing interest to obtain therapeutics proceeding of natural
sources for treatment of chronic dermatitis because of it´s high incidence and complex treatment. Objective: To
evaluate the anti-Inflammatory effect of an extract of Mangifera indica L. (Vimang cream 1.2 %) in patients with
chronic dermatitis. Material and Methods: This was a phase II, double-blind controlled trial in 30 outpatient
dermatology service with adult chronic dermatitis who were diagnosed by biopsy, personal history, pattern,
evolution, and skin lesions and who were randomized to apply an extract of Mangifera indica L. (Vimang®
cream) or placebo cream twice daily. Clinical evolution, photography, and dermatitis assay Score index (DASI)
scoring were done at baseline and after 6 weeks. Results: Fifteen subjects each received Vimang or placebo.
Post intervention, there was a difference of 30 % of improvement of Vimang group, it was significantly superior
(10:1, p = .0028; 95% CI, 0.01-0.73). 51 % of Vimang subject remained positive at the end of study.
Conclusions: The treatment with an extract of Mangifera indica L. (Vimang® cream) may be useful in the
treatment of chronic dermatitis, because of the high proportion of patients with satisfactory and partially
satisfactory response; besides it was an innocuous product for topic treatment.
This works was development the last year in the Department of Biomedical Research, Center of Pharmaceutical
Chemistry.
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PCP-03
EFFECTS OF SURFACEN IN LOW AND MAINTAINED DOSE IN THE TREATMENT OF ARDS.
PRELIMINARY RESULTS.
Barrese Y1, Avila Y1, Díaz E2 y Fernández-Limia O2
Centro Nacional Coordinador de Ensayos Clínicos (CENCEC), Calle 200 Esq 21, Atabey. Playa, Ciudad de La
Habana, Cuba. E-mail: [email protected]
2
National Centre for Animal and Plant Health (CENSA) Carretera de Jamaica y Autopista Nacional, San José de
las Lajas, La Habana, Cuba
1
The acute respiratory distress syndrome (ARDS) is caused by a pulmonary inflammation with plasma transudate
and destruction of pulmonary surfactant, causing a disruption in the exchange of gases with high mortality, the
application of exogenous surfactant improves oxygenation but has not yet been able to demonstrate
convincingly reduced mortality or other variables such as length of stay in ICU or mechanical ventilation. Our
objective was to evaluate the effect of Surfacen in low dose and sustained for three days on the oxygenation of
patients with ARDS. To this end we conducted a controlled randomized, multicentre clinical trial, in 39 adult
patients of whom 21 were treated with 100 mg Surfacen 3 times for 3 days and 18 remained only with
mechanical ventilation. The response was assessed as positive when the ratio PaO2/FiO2 ≥ 200 at the end of
treatment, we also considered the followings evaluations: gasometry, Clinic and X ray, safety, mortality, days of
stay in ICU, days of intubation and days with mechanical ventilation. The results showed a better response
indicator PaO2/FiO2 in the treated group (P = 0.0560) and if included 3 patients who did not reach the end of the
evaluation period this difference was maintained (P = 0.0411). There were no differences in mortality (P = 0.296)
among groups, 11 patients died in the Surfacen group and 6 in the control group, however the causes of death
were not by ARDS but because the basic pathology. The treated group showed a decrease (P = 0.0341) of 7.23
in the days of endotracheal intubation, there was not difference in the days of mechanical ventilation (P = 0.132)
or stay in the ICU (P = 0.108) although there was a tendency to be better in the group treated with Surfacen. We
conclude that the use of surfactant in ARDS improved oxygenation in patients with lower FiO2 requirements,
reduced the time of endotracheal intubation and had a good safety.
PCP-04
LEPIDIUM MEYENI (MACA). IT’S EFFECTS ON SEXUAL DESIRE AND SERUM REPRODUCTIVE
HORMONE LEVELS IN A GROUP OF MALES OVER 50 YEARS OF AGE.
Valdés Carrillo R*, Trasanco Delgado M*, Becerra N*, Tur Behar R**
*“Abel Santamaría Cuadrado”, Provincial Hospital. Pinar del Río. **Servier Laboratories. Medicaribe SA. Email:
[email protected]
Introduction: Lepidium Meyeni (MACA) is used from ancient times due to its supposed aphrodisiac properties.
The present study is aimed at determining if the administration of MACA during 12 weeks improves the sexual
desire in males over 50 years of age being treated with this product or placebo and the blood reproductive
hormone levels. Method: A randomized - double blind controlled by placebo design. Forty males between 50
and 59 years old who were given 1500 mg of gelatinous MACA or placebo over 12 weeks were studied. They
were valuated at 0, 2, 4, 8 and 12 weeks of treatment, valuating the sexual desire using a questionnaire and the
levels of LH, FSH, prolactin (using the Irma method), testosterone and estradiol (E2) by RIA. Results: The
analysis of logistic regression shows that MACA has an independent effect on sexual desire at 8 and 12 weeks
after treatment. The levels of hypophyseal reproductive hormone (LH, FSH) after and before treatment were not
changed during the 12 weeks using this product. Prolactin dosage showed a light and a non significant increase
(536, 5 ± 212 ng / ml) with any anxiety or depression symptoms as a result of hyperprolactinemia. The levels of
testosterone had a non significant increase. (5, 33 ± 4, 37 mU / ml). Estradiol (E2) showed a decreased in the
values which are considered in males. (1, 09± 0, 74 ng /ml). Conclusions: It is proved that in males supposedly
healthy, MACA improves the sexual desire not affecting the hypophyseal reproductive hormone levels,
testosterone showed high levels (a transitory hyperprolactinemia was observed using this product). Further
studies for validating which was found in the present study are needed.
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PCP-05
EVALUATION OF EFFICACY AND SECURITY OF DERMOFURAL OINTMENT IN PATIENTS WITH
SQUAMOSA AND CORPORIS TINEA PEDIS.
Ruiz Calabuch H1, Jiménez J2, Silva J2, Ramos L1, Leon O1, Betancourt J2.
1
Dr Faustino Pérez Hdez" Faculty of Medical Sciences. Department of Clinical Trials. Circunvalante Norte s/n.
Olivos 3. Sancti Spíritus. CP 60100. 2¨Camilo Cienfuegos¨General Hospital, Sancti-Spiritus. E-mail:
[email protected]
Dermofural is a derivative of sugar cane with antimycotic activity demonstrated in vitro. An open, parallel,
controlled and randomized clinical trial was made in 60 patients with a diagnosis of squamosa and corporis tinea
pedis, coming from the consultation of Dermatology of the "Camilo Cienfuegos" Hospital of Sancti Spíritus, with
the objectives of evaluating the clinical effectiveness, as well as the security of the product. Two groups of 30
patients were made up. Dermofural 0,15% was applied topically in ointment to the first one and ketoconazol
2% to the control, in both for a period of 6 weeks of treatment. The clinical and mycological evaluation variables
were used at the beginning and the end of the treatment, establishing the results in cured and not cured. We
observe that of a total of 39 patients that finished the final evaluation in both groups. 21,1% of cure was obtained
with dermofural and 15% with ketoconazol; of these 72,2% had negative mycotic cultures with the drug in study
and a 85,7% with the control. A percent of cure of 11,1% was found with dermofural and 19% with ketoconazol
with a signficant difference. A total of 10 adverse events occurred in 5 patients treated with dermofural (27,7%)
and none with the control product. The most common reactions were pruritus and erythema, classified as severe
and related with the product in the first case, and as slight and not related in the second. It is concluded that
dermofural is a product with an antimycotic effect and with a low toxicity, useful for the treatment of these
affections.
PCP-06
USE AND APPLICATION OF GARLIC (ALLIUM SATIVUM L) IN VIRAL AFFECTIONS ON THE SKIN.
Marin N, López K, Sánchez to, Vargas R.
Hospital: "Julio M. Aristegui Villamil". Autopista Cárdenas-Varadero, Km. 2. Cárdenas, Matanzas
We have selected the properties anti-viral of garlic (Allium Sativum L) to prove them in patients that visit to the
dermatology consultation for different affections in skin and where they are diagnosed that they are infected with
a viral etiology, specifically the flat warts, the condilomas acuminate and the simple herpes I and II. Our
objective are demonstrate the utility of the garlic in this affections, identify the signs and symptoms of the
affected patients', decide the beginning of the improvement of the patients, as well as compare the effectiveness
of the treatment of the 20% garlic’s dye with the 33% acid tricloroacético in the flat warts, with the 25% podofilina
in the condilomas acuminate and with the cream aciclovir in the simple herpes I and II. Took place a longitudinal
and prospective study using two groups for each affection, where in all the cases the group A corresponds with
20% hydro alcoholic solution of garlic and the group B whit the patients treaties with the conventional
medications for each affection. It is evidenced an improvement as a result around the 10mo day in the
condilomas acuminate and the simple herpes I and II and to the 5to day in the patients with warts flat treaties
with dye of garlic to 20%. We have given ourselves the task of proving this formulation like alternative for The
Natural and Traditional Medicine Program since we don't count with enough anti-viral to combat these affections
in the current moments.
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PCP-07
THE SAFETY AND EFFICENCY OF 20% HERBAL GUACAMAYA FRANCESA IN THE TREATMENT OF
INTERDIGITAL EPIDERMOFOSIS INFECTION.
Galbán M, Medina A, Pérez M, Moreia A, Liste S, Artiles M, Santos N, Suero B, Zaita Y, Martínez B,
Bauta R, Orozco M.
Medicine University “Dr.Serafin Ruiz de Zárate Ruiz”, Faculty of Health Technology “Julio Trigo López”.
Carretera de Báez and Circunvalacion. Santa Clara. Villa Clara. Cuba. Telef: 0142-274211 / 0142-271567. Email: [email protected]
One of the most common pathogens in our midst are the infections produced by fungus, within them includes
interdigital macerated epidermofosis (EMI) which frequently is resistant to conventional treatment Imidazole
results to a very expensive and undesireable side-effects, for this is necessary to discover a new alternative
traditional medicine called The Guacamaya Francesa that is reported like a very efficient 20% antifungal herb.
This investigation evaluates the safety and efficiency, of 20% Herbal Guacamaya Francesa in the treatment of
IME with this, it determines the mycology and clinical results or reports of patients, duration of treatment, and
possible sides-effects that develops during a pilot studies that compares 20% of herbal Guacamaya Francesa
and 2% ketoconazole cream in IME of this, opens a heighten, monocentric experimental designs which
included 2 study groups out of a total of 15 patients diagnosis, which the consultant Podology of the Faculty of
Health Technology in Villa Clara in the period of September-December 2008.The results demonstrated that a
controlled group, the efficiency was 88% while the study-group of the 20% herbal Guacamaya Francesa
obtained 75%.They did not observed any adverse reactions for all the previous were passed, the safety in
efficiency of 20% herbal Guacamaya Francesa as an alternative treatment for the IME.
PCP-08
EFFECTIVENESS AND SEGURITY OF THE DYE OF PEDILANTHUS TITHYMALOIDES (L) POIT (ITAMO
REAL) IN THE TREATMENT OF EDEMATOUS CHRONIC GINGIVITIS.
Ortiz Y, 1 Martínez H, López T, Cabrera Z
1
Universidad Médica de Granma Filial de Ciencias Médicas “Dr. Efraín Benítez Popa”.
Carretera Central Vía Santiago de Cuba Km 3 ½. Bayamo, Granma. E-mail: [email protected]
Introduction: Pedilanthus tithymaloides (L.) Poit is widely known as "itamo real" and numerous therapeutical
applications have been attributed to it. The objective of this work is to validate effectiveness and segurity of the
dye of Pedilanthus tithymaloides (L) poit (itamo real) in the treatment of edematous chronic gingivitis. Methods:
A therapeutic clinical test was made in IIb fhase, randomized, to blind person by third, unicentral; using in the
experimental design patient carriers of the disease and that decided to cooperate with the test; which were
distributed in two groups in parallel: the Group A, that received treatment with the product studied, and Group
B, that used watery clorhexidine-0.2% treatment; at the prosthodontic service of “General Manuel de Jesús
Cedeño Infante” of Bayamo. The evaluation of the clinical evolution was made a specialist in prosthodontics
using the macrocospic method, at 15 days and the month of treatment. Adverse events were identify by physical
examination and interview. Results: Female sex in both groups prevailed, in the ages between 15 and 19 years,
with moderate degree injury, which needed about 4 to 5 applications of the dye obtained to diminish or
disappear the signs and clinical symptoms. 83.63% of patients cured in group A, 73.63% with clorhexidine. In 5
patients who received itamo real-20% presented slight adverse events, such as burning and fleeting diminution
of the perception of the taste, none case entailed the interruption of the treatment. Conclusions: the analysis of
the data of the patiens that completed both period of treatment, Itamo real-20% dye and Clorhexidine -0.2%,
showed that the treatments were equivalent in the clinical healing. Both treatments turned out to be safe to take
shelter a minimum number of adverse events to the doses and according to the applied scheme of
administration in the study.
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CARTELES
ESTUDIOS
ETHNOFARMACOLOGICOS
ETHOPHARMACOLOGICALS AND USES STUDIES.
Y
DE
UTILIZACION
/
POSTERS
PEP-01
MEDICINAL PLANTS UTILIZED IN RURAL COMMUNITIES OF THE ANDEAN PIEDMONT, VENEZUELA:
TRADITIONAL USES AND RELATIVE IMPORTANCE.
Bermúdez A.1, Oliveira M.2, Carrillo T.1
1
Universidad de Los Andes. Núcleo Universitario Rafael Rangel. Departamento de Biología y Química. Grupo
de Investigación en Productos Naturales. Trujillo. Venezuela. [email protected], [email protected]
2
Universidad Simón Bolívar. Laboratorio de Biología Evolutiva y Biodiversidad. Caracas. Venezuela.
[email protected]
The medicinal plants constitute important resources in rural communities, where the traditional medicine
occupies a central role as a strategy of primary attention of health (Tabuti et al., 2003; Bermúdez, 2007). This
study aims to register the traditional uses and estimating the relative importance of the medicinal plants in rural
communities, of the Andean piedmont, Venezuela. The ethnobotanic inventory was conducted in three
communities of Monay's Plains, Trujillo state. The ethnopharmacologic information was registered utilizing the
TRAMIL survey (Robineau, 1995), while the relative importance was evaluated utilizing index of level of
significant use (Robineau, 1995) and pharmacologic versatility (Bennett and Prance, 2000). 118 species, which
belong to 106 genders and 54 families were registered, those were utilized to treat 72 local problems of health,
within 16 categories of diseases. A significant correlation between the local incidence of the categories of
diseases and the average of utilized plants to treat them was found. The majority of the traditional treatments
are prepared with the aerial parts of the plants by means of decoction, to be administered orally. Only 05
species evidenced significant uses, while 10 presented high pharmacologic versatility. The results suggest than
at this region, the use of plants still constitutes an alternative in the primary attention of health, as it becomes
evident in the number of registered species and the existent relation between the riches of the local
pharmacopoeia and the incidence of diseases.
References:
Bennett B.; and G. Prance. 2000. Introduced plants in the indigenous pharmacopoeia of northern South
America. Economic Botany. 54(1): 90-102.
Bermúdez, A. 2007. Plantas medicinales del estado Trujillo, Venezuela: Usos, ecología y estrategias de
conservación. Tesis Doctoral. Universidad Simón Bolívar. Venezuela. 234 p.
Robineau, L. 1995. Hacia una farmacopea caribeña. Enda-Caribe, UAG y Universidad de Antioquia. Santo
Domingo. 430 p.
Tabuti J. R.; Lye K. A. and S. Dhillion. 2003. Traditional herbal drugs of Bulamogi, Uganda. Plants, uses and
administration. Journal of Ethnopharmacology. 88: 19-44.
PEP-02
UTILIZATION OF MEDICINAL PLANTS IN AN URBAN COMMUNITY OF THE TRUJILLO STATE,
VENEZUELA: TRADITIONAL USES, RELATIVE IMPORTANCE AND PATTERN OF DISTRIBUTION OF THE
TRADITIONAL KNOWLEDGE.
Carrillo T.1, Bermúdez A.1, Oliveira M.2
1
Universidad de Los Andes. Núcleo Universitario Rafael Rangel. Departamento de Biología y Química. Grupo
de Investigación en Productos Naturales. Trujillo. Venezuela. [email protected], [email protected]
2
Universidad Simón Bolívar. Laboratorio de Biología Evolutiva y Biodiversidad. Caracas. Venezuela.
[email protected]
The medicinal plants are local resources largely utilized in rural communities of the Andean region of Venezuela
(Bermúdez, 2007; Carrillo and Moreno, 2006), but little is known about his use among the urban population. In
this study the traditional uses, the relative importance and the pattern of distribution of the knowledge of
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medicinal plants, were evaluated, in an urban community of the Trujillo state, Venezuela. The
ethnopharmacological information was registered utilizing the TRAMIL survey (Robineau, 1995), the relative
importance was estimated by means of index levels of significant use (Robineau, 1995 ) and the pattern of
distribution of the traditional knowledge was evaluated using Chi-square test ( p 0.05 ). 37 species, which belong
to 28 botanical families, were utilized to treat 22 local problems of health, within 08 categories of diseases. The
majority of traditional treatments is prepared with the aerial parts of the plants by means of decoction, and is to
be administered orally. Only 04 sorts evidenced significant uses. No differences were found between the
averages of the number of plants quoted by sex, but older people, above 50, possess a higher level of traditional
knowledge. These results suggest than in urban communities of the Trujillo state, the utilization of medicinal
plants for primary attention of health has died off, as compared with rural communities, and that the traditional
knowledge is distributed preferentially among elderly population.
References
Bermúdez, A. 2007. Plantas Medicinales del Estado Trujillo, Venezuela: Usos, ecología y estrategias de
conservación. Tesis Doctoral. Universidad Simón Bolívar. Venezuela. 234 p.
Carillo, T. y Moreno, G. 2006. Importancia de las plantas medicinales en el autocuidado de la salud, en tres
caseríos de Santa Ana, Trujillo, Venezuela. Revista de la Facultad de Farmacia. 48(2): 21-28.
Robineau, L. 1995. Hacia una farmacopea caribeña. Enda-Caribe, UAG y Universidad de Antioquia. Santo
Domingo. 430 p.
PEP-03
A PHARMACOECONOMIC STUDY COMPARING A NATIONAL NATURAL TOPIC PRODUCT WITH A
FOREIGN TOPICAL CREAM
Alfonso K.1, Collazo M.2, Fernández M.1, Ballagas C1
National Center Coordinator of Clinical Trials (CENCEC). 200 St, e/ 19 and 21, Atabey, Playa. Havana, Cuba
2
Center for the State Control of the Quality of Drugs (CECMED). 200 St, No. 1706, e/ 17 and 19, Siboney, Playa,
Havana, Cuba.
1
A pharmacoeconomic analysis of two topical treatments was carried out in a national clinical research phase III,
with two study groups, one treaty with ozonized oil (OLEOZON®, a national product whose source materials are
of natural origin) and another with mupirocin cream (BACTROBAN®, imported product). A total of 136 patients
were enrolled (88 patients elegible). A clinical and bacteriological diagnosis was made as a method of
confirming the disease. In the infected areas of the skin a topical ambulatory treatment with mupirocine (control
group) or ozonized oil was applied for 10 days. An initial, intermediary (after 5 days), and a final (after the
treatment) assessment was completed. A clinical and bacteriological assessment was made for determinate the
efficacy of each treatment. There were analyzed the costs of each treatment data outlining as objective the
determination of the most efficient alternative for the therapy of impetigo. Efficacy and the most direct costs in
both therapies were evaluated and it was carried out a pharmacoeconomic analysis type cost-efficacy. As result,
the treatment with the national drug was effective as an adequate therapy in the management of patients with
impetigo, and the other hand, it turned out to be the alternative of more efficiency in this therapy with a
significant difference in cost reduction (about 30 % than mupirocin cost). With the use of national product is
significativaly reduced the cost of this pharmacotherapy.
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CARTELES ESTUDIOS TOXICOLOGICOS / POSTERS TOXICOLOGICALS STUDIES
PTS-01
HYPOGLYCEMIC ACTIVITY AND ACUTE TOXICITY OF FOUR PLATS POPULARLY USED IN CUBA.
Monteagudo E1, Boffill M1, Betancourt E1, Quesada D1, Blanco F1, Díaz L1, Verdecía B1, Iglesias N1.
Experimental Toxicology Unit, Medical College of Villa Clara. Circunvalation Rd. and Acueduct Rd.. Santa
Clara, Villa Clara, Cuba. P.O.: 50200 E-mail: [email protected]
Introduction: it is well known that is feasible the support of natural drugs to synthetic and semisynthetic ones
and there is a universe of medicinal plants used traditionally in Cuba which are attributed hypoglycemic activity
based only in informations of empical use which means whitout any scietific experimental evidence, some of
those plants are: Catharantus roseus, Rhoeo spathacea, Parthenium hysterophorus and Orthosifon aristatus.
Materials and Methods: Plants were cultivated, colected and the extracts were made according to the
regulations of Agriculture Ministery and later the extracts were determined their phytochemical composition.
Hypoglicemic effect was studied in two groups (Treated and negative control: SSF 2 ml/animal of 10 animals
each one) of normoglycemic male SD rats, glycemia levels were measured before and after the three days
administration of the aequous extracts (400 mg/kg BW based on total solids). Acute oral toxicity was carried our
according to the alternative CTA limit dose method (2000 mg/kg BW) as stated in OECD Test guideline 423.
Results: the extracts showed no hypoglycemic effect a the dose essaied when compared to non treated
animals, coinciding with the existing ethnomedical data and showed no symptoms or signs of toxicity.
Conclusions: this four plants have no hypoglycemic activity and can be considered as non toxic under the
experimental conditions applied.
PTS -02
TOXICOLOGICAL EVALUATION OF POWDER OF DE PARTHENIUM HYSTEROPHORUS, L; USING
ALTERNATIVES TWO TECHNIQUES.
Saucedo Y1, García D1, Tenorio E2, Monteagudo E3.
1
Pharmacy Department. Chemistry Pharmacy Faculty. Central University. Santa Clara. Villa Clara. Cuba.
2
Chemical Bioactive Center. Santa Clara. Villa Clara. Cuba.
3
Experimental Toxicology Center. Santa Clara. Villa Clara. Cuba.
Introduction: Were carried out toxicological acute studies of the powder of Parthenium hysterophorus, L, used
plant in el treatment of the parasites intestinal. In the literature is described that continuous exposition which the
powder plant can cause toxic effects, such as contact dermatitis, photo dermatitis and rhinitis. Materials: Were
developed two alternatives assays, the oral acute oral by the class method (T.C.A)1 and were used Sprague
Dawley rats dosing unique doses of (50 mg/Kg, 300 mg/Kg, 2000 mg/Kg.p.v.) of powder plant aqueous
suspension. Was evaluated the ocular irritability of extract in concentration interval of 0.3833 mg/mL to 1 mg/mL
by the hemolytic assay which human red cells (RBC). Results: The oral (TCA) showed a DL 50 higher to 2000
mg/Kg and was classified the assay sample as non toxic. The ocular evaluations allowed classify the extract as
ocular irritant. Conclusions: The unique oral doses of powder plant, demonstrated that the DL 50 is higher to
2000 mg/Kg.p.v, and was classified as non oral toxic and was classified as ocular irritant according the
alternative assay. Bibliographic references: 1. OECD. Guidelines for testing of chemical. Acute oral toxicity,
No.401, October 2000, in http://www.oecd.org.
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PTS-03
TOXICITY STUDY TO A REPEATED DOSE OF THE AZADIRACHTA INDICA A. JUSS PLANT (NIM´S TREE).
Berenguer CA, Alfonso A, Fong O, Domínguez A, Betancourt JE, Laramendi D, Salas H, Puentes E,
Wawoe N, Cedeño N.
Centro de Toxicología y Biomedicina (TOXIMED). Autopista Nacional Km 1 ½. Teléfono: 53-22-643796, Email:
[email protected]
Azadirachta indica A. Juss plant, know as Nim´s Tree has multiple applications for agriculture, veterinary
medicine and health, is a vegetable specie with a relevant importance in the use as antimicrobial, anthelmintic,
and immunostimulating drug and others, is introduced in our country in the first decade of the XX Century and
generalized in 1990. For such motive it was realized a toxicity assay to a repeated doses using a decoction of
this plant, administering 1000 mg/kg's dose orally to Sprague Dawley rats during 28 days to evaluate the toxicity
and to establish the possible organic and functional injuries caused in the animal model referred, according to
guide 407 of the OCDE's Guides. Clinical signs and corporal weight was evaluated in the animals under
consideration, in the same way the anatomopathology, histopathology haematology and blood biochemistry
studies. The haematology and biochemists exams did not show significance alterations in the biological
parameters, keeping within the normal ranges of specie, corroborating the results obtained in the
histopathological analysis.
PTS-04
GENOTOXIC EVALUATION IN VITRO OF ERYTHROXYLUM MINUTIFOLIUM, AN ENDEMIC CUBAN
PLANT.
Leyva O1, Alonso A2, Rodeiro I3, Díaz A2, Rosario L4, Carro S2, Gonzalez-Lavaut A1
Laboratorios Biológicos Farmacéuticos, Avenida Independencia, Km. 16½, Boyeros, Ciudad Habana, Cuba.
2
Centro de aplicaciones Tecnológicas y Desarrollo Nuclear, Apartado Postal 6122. Calle 30, # 502, e/ 5ta y 7ma,
Miramar, Playa, Ciudad Habana, Cuba.
3
CEBIMAR, Loma 37, Vedado, Ciudad Habana, Cuba.
4
Centro de Química Biomolecular, ave 200, esquina 21, Atabey, Playa. Apdo. Postal 6990, Ciudad Habana,
Cuba.
1
Plants have been used since remotes times for the treatment of different diseases. Under the name of
Erythroxylum or Erythroxylon like genus, in Cuba are described 22 species. Some of these plants have been
used in the traditional medicine like anti-inflammatory in the cure of respiratory diseases. In this work the
cytotoxic and genotoxic potential of an etanolic extract of Erythroxylum minutifolium var Griseb (ExM) was
analized using a modified protocol of the SOS Chromotest and the Comet assay. Results showed that E.
minutifolium extract was cytotoxic using E. coli strain PQ37 at the concentration of 200ug/ml in presence and
absence of S9 mix activation system.The extract no induced primary DNA damage in the cells of E. coli PQ37 at
neither of the tested concentration using the SOS Chromotest assay. However, at the concentration of 100ug/ml
in presence of metabolic fraction and 200ug/ml without and with addition of metabolic activation system, the
extract was genotoxic in the Comet assay. Results obtained can be influenced by the extract composition.
Further investigations regarding dose-response-relationships and other aspects relevant for extrapolation to
human exposure seem necessary.
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PTS-05
ASSESSMENT OF THE POTENTIAL GENOTOXIC EFFECT OF AQUEOUS EXTRACTS FROM
BRYOTHAMNION TRIQUETRUM, HALIMEDA INCRASSATTA AND HALIMEDA MONILE SEAWEEDS,
USING SOS CHROMOTEST ASSAY.
Sánchez-Lamar A1, Cuevas A1, Alonso A2, Almeida E2, Verhnes M2.
1
Depto. de Biología Vegetal, Facultad de Biología, Universidad de La Habana. E-mail: [email protected]
Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear (CEADEN).
The seaweeds have attracted much attention as a source of natural preparations, which could find applications
in the cosmetic industry, food technology and biomedicine. Bryothamnion triquetrum, Halimeda incrassatta and
Halimeda monile are seaweed species whose aqueous extracts possess antioxidant properties. Nevertheless,
as preclinical requirement, the employment of these natural sources should be studied from the genotoxic point
of view. Because the knowledge about genotoxic properties of this seaweeds is limited, in the present work were
analyzed the genotoxic effects of the aqueous crude extracts of the referred seaweed species. SOS chromotest
assay, in its fluorescent variant, was used as genotoxic test. E. coli PQ37 cells were treated with different
concentrations (0.1-2 mg/mL) of the extracts, during 30’. Then, the activity levels of β-galactosidase and alkaline
phosphatase were taken as indicative of genotoxicity and citotoxicity, respectively. The results evidenced that
the extracts were not genotoxic in this experimental conditions. Moreover, in E.coli cells treated with Halimeda
monile extract, were observed SOS-induction factor levels significantly smaller that the obtained in not treated
cells.
2
PTS-06
TOXICOLOGICAL AND PHARMACOLOGICAL PROPERTIES OF A LOW MOLECULAR WEIGHT
FRACTION FROM ZOANTHUS SOCIATUS (CNIDARIA) ASSESSED IN OF-1 MICE.
Dany Domínguez Pérez1, Carlos Manlio Díaz García1, Héctor Pérez Saad2, Neivys García Delgado1, Carlos
Varela3, Yusvel Sierra4, Deyanira Fuentes5, Acela Pedroso6, Adela Rodríguez5, Marcia Hiriart7, Olga
Castañeda Pasarón1
1
Dpto. Biología Animal y Humana, Facultad de Biología, Universidad de La Habana
2
Instituto de Neurología y Neurocirugía, MINSAP
3
Acuario Nacional de Cuba
4
CEP, Facultad de Biología, Universidad de La Habana
5
Instituto de Química, Universidad Nacional Autónoma de México
6
Instituto de Endocrinología, MINSAP
7
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México
Introduction: In the group Zoantharia, belonging to the phylum Coelenterata, the presence of toxin producing
organisms have been described. The high affinity and specificity of such toxins make them interesting in basic
and applied research. Objective: To purify Zoanthus sociatus crude extract by gel filtration (Sephadex G-50), to
assess the effects of the low molecular weight fraction by a behavioral test in OF-1 mice and to determine the
mortality-dose curve of acute toxicity. Materials and Methods: The specimens of Zoanthus sociatus were
collected in the pools of the Nacional Acuarium of Cuba in September of 2008. The total body was homogenized
in a Waring-Blender by autolysis in distilled water. The crude extract was filtered and then centrifuged, the
supernatant was then lyophilized. After the purification by gel filtration (Sephadex G-50), a RP-HPLC
(Ultrasphere-DABS C18 column, Agilent 1100 system) was assessed to the low molecular weight fraction, which
effects were assayed by an Irwin test in OF-1 mice and its LD50 was calculated. Results: The low molecular
weight fraction contains peptidic compounds mostly, and it also represent the major component of the crude
extract. The pharmacological effects depend on the dose and the inoculation pathway; by the systemic way, the
alterations are mostly autonomic. The LD50 was of 132 mg/Kg when it was inoculated intraperitoneally, and it
reaches 100 % of lethality at a dose of 150 mg/Kg. Conclusions: Low molecular weight peptides in Zoanthus
sociatus crude extract have neuroactive an autonomic effects depending on the dose and administration
pathway and it is a moderate toxic fraction when it is administered intraperitoneally in mice.
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CARTELES ESTUDIOS DE MEDICINA ALTERNATIVA / POSTERS ALTERNATIVE MEDICINES
PAM-01
USE OF CHELLIDONIUM® (A HOMEOPATHIC MEDICINE) FOR THE TREATMENT OF ICTERUS IN CATS
Zafar1 MA, Mahfooz1 A, Abbas2 RZ, Sohail1 M and Manzoor1 A
1
Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad-38040-Pakistan E-mail:
[email protected]
2
Ethnoveterinary Research and Development Centre, Department of Parasitology, University of Agriculture,
Faisalabad-38040-Pakistan.
This case report is about the successful treatment of cats suffering from jaundice. Icterus (jaundice) is meant for
the yellow pigmentation in the blood and tissues. It mostly occurs due to high levels of chemical bilirubin in
blood. The presence of fleas, lice or ticks is the predisposing factor for jaundice in animals. In cats, in addition to
these factors, other risk factors include infection with feline leukemia virus or feline infectious peritonitis virus.
Two cats (with a history of jaundice) were brought to the clinic of medicine and surgery, University of agriculture,
Faisalabad-Pakistan. Firstly, the diagnosis was confirmed by physical examination and by performing laboratory
examinations for complete blood count and liver function test. After diagnosing, these cats were treated with
Chellidonium® drops (a homeopathic medicine) 30 potency through oral route, two drops three times a day for
10 days. During this duration the supportive therapy was also continued. After 10 days, complete blood count
and liver function tests were again performed which showed positive results towards recovery, the mucous
membranes and sclerae became normal and animal showed good recovery in its health status as well. This lead
us to the conclusion that icterus in animals could be overcome with the help of Chellidonium® drops orally along
with supportive therapy parentally.
PAM-02
APPLICATION OF THE MAGNETIC INDUCTIVE STABILIZER (EIMA®) AND OZONETHERAPY
TREATMENT OF PATIENTS WITH DISC HERNIA
IN THE
Yepes A1, Aguilera E2, Gómez R1, Martínez I1, Aguilar B1, Kindelán L2.
Centro de Terapias Metabólicas. Guerrero No.4. Banderilla. Veracruz. México.
2
Departamento de Farmacia. Universidad de Oriente. Patricio Lumumba s/n.
CP: 90 500 Santiago de Cuba. Cuba. E-mail: [email protected], [email protected]
1
Introduction: At present there isn’t an evaluation of treatment with magnetic inductive stabilizer (EIMA®) and
ozone therapy in patients with disc hernia. Objective: To evaluate the effect of the therapy with EIMA® and
ozone in patients with disc hernia. Materials and methods: It was carried out a descriptive , retrospective study,
not randomized in 33 patients assisted in the Center of Metabolic Therapies of Banderilla, Veracruz, Mexico, in
the period of January to July 2008. An inductive electromagnetic field was applied during 21 sessions with ozone
therapy. Results: The biggest number of patients included in the study corresponded to the masculine sex (17
patients) representing 53.1% of the total of the sample, prevailing the group of 46-60 year-old age. Among the
most frequent symptoms is stood out the pain in the lower back and legs in 20 and 10 patients respectively, as
well as the difficulty for walking (7 patients) and paresthesia in the legs and pain in the gluteal region, both in 5
patients. 71 symptoms were reported at the beginning of the study to diminish up to 20 after the treatments with
EIMA® and ozone. About 85 % of the total of patients was reported as improved, 15 % continued equally and
none increased during this period. Conclusions: The treatment with EIMA® and ozone was useful to achieve
the reduction of the symptoms of patients affected with DH, diminishing significantly starting from the 10th
treatment session.
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PAM- 03
ACUPOINT INJECTION WITH MINIMAL DOSES OF STEROIDS FOR JOINT PAIN RESISTANT.
Perdomo Delgado J1, González Pla E2, Castro Morillo E3, Páez Casona T3, Sánchez Hernández T2.
Provincial Department of Traditional and Natural Medicine. Santa Teresa 7905. Matanzas. Cuba.
2
Juan Guiteras Gener Medical Faculty. Carretera Central Km. 100. Matanzas. Cuba.
3
Provincial Center of Traditional and Natural Medicine “Dr. Mario E. Dihigo”. Navia esquina Isabel Primera.
Versalles. Matanzas. Email: [email protected]
1
A prospective, descriptive study, with inferential analysis was carried out to evaluate the therapeutic effect of
acupoint injections with minimal doses of steroids in the treatment of joint pain resistant to other therapeutic
procedures. A total of 74 patients from the Provincial Center of Traditional and Natural Medicine “Dr. Mario E.
Dihigo” of Matanzas were included, all of them having received previous allopathic and CAM treatments,
including Acupuncture. Visual Analogical Scale (VAS) was applied to evaluate pain intensity. Traditional and
Clinical diagnoses were determined for each patient and allopathic drugs consumption was also evaluated.
Based on pain topography local acupoits were punctured with minimal doses of steroids. Initial pain score was
7.9±0.53 for 74 patients while at the end it was 1,74±0,6 for only 9 patients. Only these 9 patients ended with
allopathic drugs consumption but at lower doses. According to these findings acupoint injections with minimal
doses of steroids is a recommended procedure for treating joint pain resistant to other treatments.
PAM-04
HEMOSTATIC EFFECT OF ULTRA HIGH DILUTIONS OF PHOSPHORUS ON SURGICAL PATIENTS.
Consuegra Leon L1, Perdomo Delgado J2, González Pla E3, Trimiño Galindo L1, Otero Barroso M1.
1
”Mario Muñoz” Hospital. 154 Final. Reparto Reynold García. Matanzas. Cuba.
2
Department of Traditional and Natural Medicine. Santa Teresa 7905. Matanzas. Cuba.
3
Juan Guiteras Gener Medical Faculty. Carretera Central Km. 100. Matanzas. Cuba.
An on-going randomized-triple blinded clinical trial was carried out to evaluate the haemostatic effect of ultra
high dilutions of Phosphorus in the surgical patient. Prostatectomy, hysterectomy and knee surgery were the
three surgical procedure included. Only 30 patients were evaluated as a preliminary sample, divided into three
groups which received Phosphorus 200 CH, Phosphorus 1000 CH and a placebo. Haematocrite, Coagulation
and Bleeding time were performed to every patient, previous and after medication, except Haematocrite which
was performed after surgery. Statistical tests to compare average of non-independent samples and KruskalWallis test were applied to the clinical and laboratory data obtained. Even when the haemostatic effect was not
statistically demonstrated, a lower Coagulation time with statistical significance was observed for the group
treated with Phosphorus 1000 CH and both Phosphorus groups had a lower Bleeding time but without statistical
significance. No differences on blood loss were observed and it is recommended to keep on increasing the
number of patients for this trial.
PAM-05
SURGICAL ACUPUNTAURAL ANALGESIA OF CATARACT IN GERIATRICS PATIENT
Domínguez González EJ
Medicine intern. Not graduated instructor of General Surgery. Superior Institute of Medical Sciences Santiago de
Cuba. Faculty #1. San German # 526, between Calvario and Moncada. Santiago de Cuba. E-mail:
[email protected], [email protected]
Introduction: Acupuncture is one of the oldest, most commonly used medical procedures in the world.
Nowadays this type of technique is used in eyes surgery and we want to show ours results of a new procedure
in cataract surgery. Materials and Methods : Was carried out a clinical, randomized, controlled test, phase III to
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evaluate the effectiveness of the surgical acupunctural analgesia, in geriatric patient with diagnosis of cataract
attended in the Anesthesiology service of the General Hospital "Orlando Pantoja Tamayo" in Contramaestre,
Santiago de Cuba, in the period of February to August of 2008. The Universe was conformed with 104 patients
that were assigned aleatorily to two groups, one study that was applied surgical acupunctural Analgesia, and a
second group control that was carried out with retroocular blockade. Results: The quality of the analgesia,
Arterial tension, heart frequency, breathing frequency were evaluated, complications and time of postoperative
analgesia for both groups. In most of the patients of the group I (Study) a good analgesia was achieved, with
stability of parameters and the postoperative very satisfactory with minimum complications in comparison with
the group control. Conclusions: The Surgical Acupunctural Analgesia was useful as an analgesic method in the
operations of cataract in geriatrics patient. Key words: Cataract, Analgesia, Acupuncture.
PAM-06
EFFICACY OF RESCUE REMEDY IN THE TREATMENT OF INTERMITENT MODERATE ASTHMA CRISIS.
Ferrada Zapatero D, Rodríguez Gutiérrez M, Pupo Machado M, Vila Rodríguez I, León Ramírez M, Ávila
Pérez Y, Labrada Vila I.
Las Tunas Medical University. E-mail: [email protected]
Floral extracts constitute a therapeutic system which is obtained from some particular energetic elements of
certain flowers from different plants mixed in a hydro-alcoholic solution. Edward Bach, an English doctor
discovered this kind of therapy which includes 39 floral extracts, being the Rescue remedy known as number 38,
one of the most widely used in the treatment of emergency conditions, stress, agony etc. Because of the utility in
the use of natural drugs in the treatment of the multiple conditions and necessity to validate the therapeutic
system of Bach Flowers which was aim at determining the value of this therapy to relieve moderate bronchial
asthma crisis. The study was done in patients who were assisted at the Emergency Room of Gustavo
Aldereguía Policlinics in the third trimester of the year 2008. A total sample of 60 patients was evaluated
grouping than into two groups of 30 patients each. One of the group (the control one) was treated in the
conventional way using the established treatment and the other (the study group) was treated using the Rescue
Floral Remedy, giving them four drops every five minutes till completing four doses. Fifteen minutes after
receiving the treatment 80.7% of the patients from the studied group significantly improved symptoms in contrast
with 63.3% in the control group. 93.3% of the patients from the study group did not develop any adverse
reactions contrasted with 96.7% of the control group patients who did have adverse reactions. The 39 Rescue
Remedy proved to be effective as an emergency therapy in the studied patients.
PAM-07
EFFECTIVENESS OF THE USE OF THE SAMBUCUS IN THE TREATMENT OF THE BRONCHIAL
HIPERREACTIVITY IN PEDIATRIC AGE.
Herrero Aguirre H, Álvarez G. RMª.
Policlínico Docente Municipal. Municipio Santiago de Cuba. E-mail: [email protected]
Introduction: The treatment homeopathic is used by millions of people of different continents for more than 200
years, It have great acceptance when providing an integral reestablishment in a soft way and with scarce
adverse reactions, the sambucus is a remedy of vegetable origin that allows to treat the simple bronchial
hyperactivity in a infants. Materials and Methods: A study on the evaluation of Sambucus therapeutic
effectiveness was carried out, in 1 to 5 year-old children who were assisted at the Paediatrics consult of the
Work Basic Group No.1 of the Teaching Municipal Policlinic from Santiago de Cuba, in the period of time from
January to December, 2008. A series of 46 carriers of primary bronchial hyperactivity, aimed at evaluating the
usage of the homeopathic therapy in the paediatric carrier of a bronchial hyper reactivity. Results: The results
were statistically validated by SPSS version 11.5, with 95% confidence, and within the main results we find that
87.5% of the patients solved their chesty cough with the use of this remedy and improved the symptoms, the
treatment was also cheaper and without reported adverse reactions, with the consequent economic, social and
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environmental impact that provokes this type of homeopathic therapy. Conclusions: The treatment was also
cheaper and without reported adverse reactions, with the consequent economic, social and environmental
impact that provokes this type of homeopathic therapy. Key words: Sambucus, primary bronchial hyperactivity.
CARTELES TECNOLOGIAS FARMACEUTICAS / POSTERS PHARMACEUTICALS TECHNOLOGIES
PPT-01
SOLAR DRYING CURVES FOR THREE MEDICINAL SPECIES. CURVAS DE SECADO SOLAR PARA TRES
ESPECIES MEDICINALES.
JEREZ A, CHINAPPI I, UZCATEGUI M.
Alonso Jerez Carrizo: Profesor Agregado del Núcleo Universitario Rafael Rangel. Universidad de los Andes.
Departamento de Ingeniería. Laboratorio de Energía y Mecanización Agrícola. Trujillo-República Bolivariana de
Venezuela. Teléfono: 0058-0272.4149301. Correo electrónico: [email protected]
Generate solar drying curves in medicinal plants is a valuable tool for producers and researchers and to
establish parameters for comparison with other drying methods. In this investigation, using a solar dryer, the
solar drying curves were built in actual hours and in equivalent hours of sunshine to the leaf of three medicinal
species: the white Basil (Ocimum basilicum L.), is employed as antispasmodic, Melissa (Melissa officinalis L.)
used as a sedative and Malojillo or Caña santa (Cymbopogon citratus D.C.), consumed mainly as
antihypertensive and anti-inflammatory. These species are commonly used, produced and marketed in the state
of Trujillo, andean region of the Bolivarian Republic of Venezuela. The kinetics of moisture loss as a function of
time is plotted, in three periods, until the material reached a humidity below 10%, with a temperature no higher
than 45 Celsius degrees in the working fluid. The temperature in the drying chamber was regulated by a curtain
down on the collector. Each test was replicated three times. The drying time was set as the time required to
reach the recommended moisture content of 10% and was obtained by interpolation in the drying curves.Some
parameters were determined to assess the energy converter. To generate the drying curves and compared
those with the conventional drying outdoors reported in the literature cited, was found a significant reduction in
drying time, in addition, it has the advantage of obtaining a better quality product because the material is not
exposed to direct sunlight. In assessing the solar dryer was found a good control of the drying temperature for all
cases tested.
Keywords: solar dryer, solar energy, medicinal plants, drying time, drying curves, flat solar collector.
PPT-02
METHOD OF EXTRACTION CARVONE BY DIRECT INDUCTION HEATING – ASSISTED MAGNETIC FIELD
L. Lagunez Rivera, G. Vilarem
Laboratoire de Chimie Agro-Industrielle, Ecole Nationale Supérieure des Ingénieurs en Arts Chimiques et
Technologiques, Institut National Polytechnique de Toulouse, Unité Mixte de Recherche 1010 INRA/INPENSIACET, 118 route de Narbonne, 31077 Toulouse Cedex 4, France.
Instituto Politécnico Nacional, CIIDIR-Oaxaca, México*.
E-mail: [email protected] Telephone: +33(0)562885725 Fax: +33(0)562885730
This works presents the development of a new method of hydrodistillation of essential oil of caraway (Carum
carvi L.) by Direct Induction Heating - assisted by Magnetic Field (DIHMF), as well as the development of the
required apparatus. Water and food salt (99.8% sodium chloride) were used as electrolyte solutions and at the
same time as an extractant solution. The method was tested on caraway fruits as a source of carvone and
limonene as the main components. The carvone also has a medical relevance, various works in the medical field
were recently reviewed and discussed by Carvalho et al., 2005. These authors described how carvone may lead
to better drugs and to an effective treatment for candidal infections. It was also used in the stereoselective
synthesis of the marine antitumor agent eleutherobin, this was proved a class of potential chemopreventive
anticarcinogenic agents and it was found to be helpful in the prevention of cancer.
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The essential oil of this fruits was also isolated by conventional hydrodistillation as a standard method in
laboratory and pilot scale. The isolated volatile products obtained by both methods were analysed using gas
chromatography (GC). The obtained results were then compared. Even when the apparatus and hydrodistillation
method ensures the isolation of the essential oils, as well as conventional hydrodistillation, the time required to
obtain the isolated product is remarkably shorter and not any toxicity of the solvent extractant.
KEY WORDS: hydrodistillation method; essential oil; caraway; limonene; carvone; direct induction heating;
magnetic field.
_________________________________________________________________________________________
REVISTA CUBANA DE PLANTAS MEDICINALES
RNPS 1827
ISSN 1028-4796
ACERCA DE ESTA REVISTA
Organismos patrocinadores: Comisión Nacional Asesora en Investigaciones de Plantas Medicinales del MINSAP y la
Universidad de Ciencias Médicas de La Habana.
Copyright: Todos los derechos reservados.
Frecuencia: Cuatrimestral y trimestral desde 2005.
Año de fundación: 1996
Lectores y autores: Profesionales y técnicos en el campo de la investigación de las plantas medicinales.
Misión: Una publicación científica periódica, de reflexión y debate crítico, sobre aspectos teóricos y prácticos del uso de las
plantas medicinales y los medicamentos herbarios en la salud pública, así como, un vehículo informativo del desarrollo del
pensamiento y la investigaciones en su temática.
Visión: Constituir un órgano de consulta, con enfoque científico y crítico del uso de las plantas medicinales y un referente
obligado para conocer el pensamiento, la investigación científica y el uso de las plantas medicinales.
Index: Latindex, LILACS, Imbiomed, SciELO.
Formato electrónico: HTML y PDF.
Información General:
La RCPM le ofrece a los profesionales, del campo de la salud y a sus afines, la posibilidad de publicar los resultados de sus
investigaciones; reflexiones acerca de tendencias del uso de las plantas medicinales en la salud pública y revisiones de
temas acorde con el perfil de la revista. Expertos reconocidos median el proceso de arbitraje. Autores cubanos y de otros
países poseen en nuestras páginas una tribuna abierta a todo esfuerzo por la defensa y mejoramiento de la salud de los
pueblos con el empleo científico de las plantas medicinales y los medicamentos herbarios.
Es propósito también de la RCPM, promover la salud, contribuir a la prevención de enfermedades y al mejor manejo en la
atención de enfermos, a la prolongación y al mejoramiento de la calidad de vida de individuos y colectivos humanos.
RCPM divulga información sobre actividades relacionadas con la utilización de las plantas medicinales en salud pública y
medicina social, del Sistema Nacional de Salud de Cuba y de sus programas de cooperación. También sobre lo que ocurre
en sistemas de otros países.
El contenido de RCPM se refiere a todos los temas relacionados con las plantas medicinales y los medicamentos herbarios
(botánica, etnomedicina, conservación, cultivo, fitoquímica, farmacia, farmacología, toxicología y otras).
Secciones fijas:
Editorial: refleja las opiniones y proposiciones de la Revista sobre un aspecto o problema de las plantas medicinales
relacionado con la salud pública. Será firmado por el/la autor/a que lo haya redactado, o del Comité Editorial, cuando sea
resultado de una reflexión colectiva.
Artículos originales: Informes de investigaciones originales sobre un problema de las plantas medicinales, en su acepción
más amplia. No se aceptan relatos de carácter anecdótico, ni artículos en serie de diversos aspectos de una misma
investigación.
Secciones eventuales:
Revisión: Revisiones bibliográficas sobre un tema de plantas medicinales de actualidad, por lo que las citas, generalmente
numerosas, serán mayoritariamente recientes (5 años). Tendrán un mínimo de 20 referencias.
Debate: Documentos donde el/los autor/es expresan sus posiciones o criterios, los argumentos en que se sustentan y las
conclusiones a que llegan, pero que no están en función de una investigación o revisión específica, y sí de resultados de
estos tipos de trabajos propios o de otros autores. El contenido polémico es lo característico. Esta sección puede incluir
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conferencias, entrevistas, mesas redondas, paneles, foros y simposios. Incluimos informes sobre iniciativas, proyectos,
intervenciones y reuniones nacionales o internacionales sobre temas de plantas medicinales.
Comunicaciones: Artículo breve, generalmente para informar sobre un resultado preliminar de investigación, un método o
técnica novedosa. No excederá de 2 500 palabras.
Tesis: Reseña breve sobre tesis de especialización, maestría o doctorado en materia de salud pública, recientemente
defendida y su localización.
Eventos: Anuncia u ofrece breve cuenta de congresos u otro tipo de reunión científica, de próxima o pasada realización,
afines a la Revista.
Obituario: Informa sobre el deceso reciente de alguna personalidad relevante de la salud pública y especialmente
relacionada con el tema de las plantas medicinales.
Efemérides: Recuerda alguna fecha de significación en la historia de las plantas medicinales.
Noticias: Divulga algún hecho de importancia relativo al contenido y perfil de la Revista.
Lecturas: Recomienda artículos de revistas, u otras publicaciones recientes sobre temas de las plantas medicinales o
afines.
Libros: Presenta una síntesis y comentarios de libros de aparición reciente. No excederán estas presentaciones de 1 500
palabras.
Gaceta: Reproduce documentos oficiales del sistema de salud cubano, de organismos o acuerdos internacionales,
generalmente de carácter regulatorio.
Cartas: Publica cartas de los lectores, de contenido científico, sobre los temas propios de la Revista. Incluye discrepancias,
objeciones, críticas adversas o laudatorias de lo publicado en la Revista, advertencia de errores o datos complementarios.
No rebasarán las 500 palabras. Serán firmadas por su/s autor/a/es/as, que informarán su lugar de trabajo y dirección postal
y/o electrónica.
Anuncios: estarán sujetos a la aprobación del Comité Editorial, que cuidará del aspecto ético de su contenido.
CUERPO EDITORIAL
Director: DrC Francisco Morón Rodríguez
Secretaria: Dra. Ana Ibis García Hernández
Editora: Lic. Lisel Bidart Vidal
Comité editorial: DraC. Lérida L. Acosta de la Luz, DraC. Irma Castro Méndez, DrC. Víctor R. Fuentes Fiallo, Lic. Marisol
López Barreiro, Lic. Rosa Menéndez Castillo, Dra. Ileana Quiñones La Rosa, DraC. Mireida Rodríguez Acosta, DraC. Juana
Tillán Capó, MSc. María del Carmen Victoria Amador, DraC. Teresita Zambrana Alvarez, MSc. Juan D. Arbayza Fructuoso
(Perú), DrC. Ricardo O. Guerrero Jácome (Puerto Rico), Dr. José Luis Martínez Salinas (Chile), MSc. Yraida Rodríguez Luis
(Cuba)
Arbitros: DraC. María de los Angeles Boffill Cárdenas, MSc. Aida Corral Salvadó, MSc. María de los Angeles Díaz
Castellón, DraC. Milagros García Mesa, MSc. Gastón García Simón, Lic. Humberto López Pellón, MSc. Gladys Menéndez
Jorrín, MSc. Blanca del Rosario Peña Núñez, DrC. Héctor Pérez Saad y MSc. Marlene Porto Verdecia.
Traducción: Edgardo Fundora
Diseño: Luciano O. Sánchez Núñez
La revista Cubana de Plantas Medicinales es una revista arbitrada. Su arbitraje es a doble ciego, lo que hace anónimo el
importante trabajo del que depende en gran medida la calidad del contenido de la publicación. Si bien el anonimato se aplica
a que no se conoce quién revisa un trabajo determinado, sí puede informarse quiénes realizan esta tarea. Dar a conocer
nuestros árbitros es la única forma de retribución, en este caso moral, que la revista puede ofrecer.
INSTRUCCIONES A LOS AUTORES
Normas editoriales de la Revista Cubana de Plantas Medicinales (RCPM)
· Solicitamos a los autores que consulten las INSTRUCCIONES A LOS AUTORES que son comunes a
todas las revistas médicas cubanas de nuestra Editorial y que aparecen en URL siguiente:
http://bvs.sld.cu/revistas/instrucciones/instrucciones.htm
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