Protein and lipid cleaning of blue light

EAOO 2012
PROTEIN AND LIPID CLEANING OF BLUE LIGHT-FILTERING CONTACT LENS WITH
CLEANING AND LUBRICANT DROPS
Bonnin-Arias C1 , Chamorro E2, Urbano-Rodríguez R3, Sánchez-Ramos C1
1 Alta Eficacia Tecnología, Spain
2 Factoria I+D, Spain
3 Avizor Laboratories, Spain
4 Neuro-Computing y Neuro-Robotics Research Group, University Complutense of Madrid, Spain
Corresponding author: [email protected]
INTRODUCTION
MATERIALS AND METHODS
Using contact lenses is a more and more frequent, normal practice. One of
the most important problems for the contact lens wearers is the onset of
deposits adhered on the contact lens surface. There are different reasons for
these deposits: the patients’ tear film, the environment, and the contact lens
handling.
The presence of deposits involves a high-risk situation for the onset of
problems because of the modifications induced in the lens, like a decrease in
its optical quality, or the appearance of inflammation or sensitiveness signs,
which can even develop important changes in the ocular surface.
INCUBATION OF THE
CONTACT LENSES
Lenses in 1.5ml solution at
37ºC – 96h - Shake
Fig.1. Working solution Pipetting (lipids or
proteins)
PROTEIN TRIAL
As one of the most important problems in wearing contact lenses is the
deposit of lipids and proteins on their surface, it is necessary to evaluate how
this deposition degree would be in any new contact lens design, as well as
the cleaning effect of the current maintenance solutions on this new lens.
Aim: Determining the efficiency of the moisturizing drops Cleaning
Drops® (Avizor) at cleaning protein and lipid deposits on contact
lenses with optical filters blocking the short-wave length light
radiations.
0.5ml/mg serum bovine albumin
7.2µl/ml 14C-albumin
QUANTIFICATION OF
DEPOSITS
PROTEIN TRIAL
Control lenses
Not treated
RADIACTIVITY COUNTING:
Twinkling In liquid phase
Test lenses
Robbed Immersion with
moisturizing drops Avizor
Cleaning Drops (7ml, 4h)
Fig. 4. Radioactivity counting
LIPID TRIAL
Extraction method Extraction
chloroform:methanol (2:1)
High-resolution liquid
chromatography
LIPID TRIAL
0.17mg/ml free cholesterol
0.23mg/ml cholesterol Oleate
TRIPLICATE TRIALS
Iotrafilcon B Profilcon A
Fig.2. Types and classification
of the CL used
RESULTS
CLEANING
PROCESS
Fig.3. Cleaning process of the contact
lenses with the solution Cleaning Drops
Fig. 5. High-resolution liquid chromatography
Lente
de uv-blue
contactofilters
con filtro
CL with
CL with
uv-blue
Lente
de no
contacto
sinfilters
filtro
Test
CL with uv-blue filters
Fig.6. A) Albumin deposits accumulated on contact lenses with
and without uv-blue filters, treated and non-treated with the
solution Cleaning Drops B) Cleaning efficiency of the solution
Cleaning Drops for albumin deposits
There is a cleaning effect in both lenses,
greater in CL without protecting filters
Control
Test
Control
Free cholesterol
cleaning efficiency (%)
Total cholesterol
cleaning efficiency (%)
Control
CL with no uv-blue filters
CL with uv-blue filters
Fig.7. A) Total cholesterol deposits accumulated on contact
lenses with and without uv-blue filters, treated and non-treated
with the solution Cleaning Drops B) Cleaning efficiency of the
solution Cleaning Drops for total cholesterol deposits
There is a cleaning effect in
both lenses
Test
Esterified cholesterol
cleaning efficiency (%)
Test
Albumin cleaning
efficiency (%)
CL with no uv-blue filters
ESTERIFIED CHOLESTEROL
Freel cholesterol (µg/lente)
Albumin (µg/lente)
Control
FREE CHOLESTEROL
Esterified cholesterol (µg/lente)
TOTAL CHOLESTEROL
Total cholesterol (µg/lente)
ALBUMIN
CL with no uv-blue filters
CL with no uv-blue filters
CL with uv-blue filters
Fig.8. A) Free cholesterol deposits accumulated on contact
lenses with and without uv-blue filters, treated and non-treated
with the solution Cleaning Drops B) Cleaning efficiency of the
solution Cleaning Drops for free cholesterol deposits
Fig.9. A) Esterified cholesterol deposits accumulated on contact
lenses with and without uv-blue filters, treated and non-treated
with the solution Cleaning Drops B) Cleaning efficiency of the
solution Cleaning Drops for esterified cholesterol deposits
There is a cleaning effect in both lenses,
greater in lenses without protecting filters
CONCLUSSIONS
Cleaning Drops® is a cleaning system efficient to eliminate lipid and protein deposits
on contact lenses tested with and without filters blocking the short-wave length
bands.
“Viability study. Contact lenses to prevent blindness: Maintenance
and hygiene products”. AVIZOR in collaboration with Universidad
Complutense and Factoria I+D. Supported by Comunidad de Madrid
and FEDER funds.
CL with uv-blue filters
Cleaning effect only in CL with
protecting filters
REFERENCES
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